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安氏伪镖水蚤精氨酸激酶cDNA的克隆及镍胁迫下的表达特性

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厦门大学学报（自然科学版） 2013年第4期52卷 553-559页

作者：蒋洁兰王桂忠毛明光李少菁厦门大学海洋与地球学院近海海洋环境科学国家重点实验室福建厦门361102

为探讨镍胁迫对桡足类影响的分子机制,以安氏伪镖水蚤(Pseudodiaptomus annandalei)为研究对象,采用cD-NA末端快速扩增技术(RACE)方法获得了安氏伪镖水蚤精氨酸激酶(Arginine kinase,AK)cDNA全序列,全长1 180bp,开放阅读框1 068bp,编码... 详细信息

为探讨镍胁迫对桡足类影响的分子机制,以安氏伪镖水蚤(Pseudodiaptomus annandalei)为研究对象,采用cD-NA末端快速扩增技术(RACE)方法获得了安氏伪镖水蚤精氨酸激酶(Arginine kinase,AK)cDNA全序列,全长1 180bp,开放阅读框1 068bp,编码355个氨基酸残基,预测蛋白质分子质量为***分析结果显示,安氏伪镖水蚤AKcDNA与罗氏沼虾(Macrobrachium rosenbergii)、多齿新米虾(Neocaridina denticulata)和光滑双脐螺(Bi-omphalaria glabrata)AK基因具有较高的同源性(77%～82%).荧光定量PCR结果显示:低质量浓度镍(10μg/L)对AK转录水平呈现峰值效应,高质量浓度(40和500μg/L)镍则对AK呈现抑制效应,表明AK与安氏伪镖水蚤响应重金属胁迫密切相关.本研究为揭示镍胁迫对桡足类的影响机制提供理论依据.

关键词：安氏伪镖水蚤精氨酸激酶镍胁迫表达特性

锯缘青蟹脑内5-HT和NPY的免疫细胞化学定位(英文)

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水产学报 2005年第4期29卷 441-446页

作者：黄辉洋叶海辉李少菁王桂忠厦门大学海洋学系福建厦门361005 厦门大学海洋学系

为探讨5-羟色胺和神经肽Y在锯缘青蟹脑内的存在与否和分布状况,应用免疫细胞化学技术,在光学显微镜下观察5-羟色胺和神经肽Y阳性细胞和神经髓质的形态和分布。结果表明:在锯缘青蟹脑中共12个胞体群和11个神经髓质中,前脑和中脑中有4个... 详细信息

为探讨5-羟色胺和神经肽Y在锯缘青蟹脑内的存在与否和分布状况,应用免疫细胞化学技术,在光学显微镜下观察5-羟色胺和神经肽Y阳性细胞和神经髓质的形态和分布。结果表明:在锯缘青蟹脑中共12个胞体群和11个神经髓质中,前脑和中脑中有4个细胞群和6个神经髓质检出5-HT免疫阳性反应;中脑和后脑中有7个细胞群和3个神经髓质具有NPY免疫阳性反应。5-羟色胺和神经肽Y在锯缘青蟹脑内的特异性分布,为其参与神经生理活动提供了形态学证据。

关键词：锯缘青蟹脑 5-羟色胺神经肽Y 免疫细胞化学

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谷胱甘肽转移酶抑制剂的高通量筛选

药学学报 2008年第1期43卷 108-112页

作者：张丹参张天泰李韶菁杜冠华中国医学科学院中国协和医科大学药物研究所

To identify the inhibitor of glutathione S-transferase（GST）,a high-throughput screening method was established in a 384-well microplate with total 35 μL volume,and the absorbance at 340 nm is *** concentrations of substrates,CDNB and GST were determined by *** optimal enzyme kinetics reaction time and temperature are 2 h and 30 ℃,*** established model was evaluated by NaOCl,a known GST inhibitor,and the parameter Z′ was 0.77,which showed a high feasibility and stability of the assay.A total of 31 098 compounds were screened,of which 4 compounds were shown to inhibit GST activity,high inhibiting activity for their IC50 of GST inhibition was 3.94,4.05,74.85,and 77.41 mg·L-1,*** results indicated that the colorimetric method by using CDNB and GSH as substrate is stable,sensitive,reproducible and also suitable for high throughput screening.

关键词：谷胱苷肽转移酶谷胱苷肽转移酶抑制剂高通量筛选

刀额新对虾周期蛋白B基因的分子克隆及序列分析

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厦门大学学报（自然科学版） 2012年第4期51卷 753-758页

作者：杨亚男叶海辉尚丽丽黄辉洋李少菁厦门大学海洋与地球学院福建厦门361005

周期蛋白B(cyclin B)是真核生物细胞周期的一种重要调控原件,通过调节周期蛋白依赖性蛋白激酶(cyclin-dependent kinase,CKD)的活性可以控制细胞周期.运用RACE(rapid-amplification of cDNA ends)技术克隆了刀额新对虾(Metapenaeus ensis)cyclin B基因.刀额新对虾cyclin B的cDNA全长为1 681bp,5′端非编码区为111bp,3′端非编码区为355bp,开放阅读框为1 215bp,编码404个氨基酸,平均分子质量为45 767.9u,pI为***比对后发现,其氨基酸序列与斑节对虾(Penaeus monodon)的同源性达约90%.基于cyclin B序列所绘制的进化树基本上能反映出各物种间的进化关系.半定量RT-PCR结果显示,刀额新对虾的cyclin B基因在不同组织表达中具有明显的组织特异性,在鳃、卵巢和心脏中有较高的表达量,在眼柄神经节和胸神经节中表达量较低.推测该差异性主要与cyclinB在细胞周期中调控细胞分裂的功能有关.

关键词：周期蛋白B 刀额新对虾基因克隆

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长毛对虾卵子皮层反应的研究

动物学报 2001年第1期47卷 112-115页

作者：王艺磊张子平常林李少菁集美大学水产生物技术研究所福建厦门361021 香港城市大学生物与化学系厦门大学海洋学系福建厦门361005

Morphology of the cortical reaction in the eggs of Penaeus penicillatus was studied with the light microscopy,scanning electron microscopy and transmission electron microscopy. The cortical reaction is divided into four stages. These stages are unreacted stages, early stages, corona stages and dissipation stage. The cortical rods were released and formed a jelly coating around the surface of the egg. The jelly coating remained until the first cleavage had finished. In the end, the hatching membrane appeared around the egg. It is believed that these cortical reaction are responsible for the prevention of polyspermy by both a chemical and physical block and that also may establish a microenvironment inside a touch chorionic membrane for the developing embryo.

关键词：长毛对虾卵子皮层反应形态学

白内障超声乳化吸除术后角膜内皮细胞变化的研究

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国际眼科杂志 2014年第12期14卷 2247-2249页

作者：刘婕赵娴邵丽静左建霞李小磊赵欣中国河北省石家庄市第一眼科医院白内障科 050000

目的:观察不同类型白内障患者超声乳化吸除术后角膜内皮细胞的变化。方法:随机选取我院老年性白内障、糖尿病性白内障及高度近视并发性白内障患者各30例30眼,均行白内障超声乳化吸除联合人工晶状体植入手术,于术前及术后1wk采用非接触... 详细信息

目的:观察不同类型白内障患者超声乳化吸除术后角膜内皮细胞的变化。方法:随机选取我院老年性白内障、糖尿病性白内障及高度近视并发性白内障患者各30例30眼,均行白内障超声乳化吸除联合人工晶状体植入手术,于术前及术后1wk采用非接触型角膜内皮显微镜行角膜内皮细胞密度及六角形细胞比例检查。结果:三组术前角膜内皮细胞密度及六角形细胞比例比较,差异无统计学意义(P>0.05)。三组术后1wk角膜内皮细胞密度分别为2 496.86±298.96/mm2,2 379.51±375.13/mm2,2 425.38±312.68/mm2,六角形细胞比例分别为(46.20±12.03)%,(43.44±13.99)%,(44.35±8.13)%。三组术后1wk角膜内皮细胞密度及六角形细胞比例均较术前减少,其差异均有统计学意义(P<0.05)。术后各组间比较,糖尿病性白内障组和高度近视并发性白内障组术后均较老年性白内障组术后降低,其中糖尿病性白内障组术后角膜内皮细胞密度和六角形细胞比例降低较明显,与老年性白内障组比较,其差异有统计学意义(P<0.05)。结论:糖尿病性白内障及高度近视并发性白内障患者角膜内皮对超声乳化手术的耐受性降低,对角膜内皮细胞应行准确术前评估及术中保护。

关键词：白内障超声乳化手术角膜内皮细胞糖尿病高度近视

An approach to the study of copepod egg banks based on efficient DNA extraction from individual copepod eggs

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An approach to the study of copepod egg banks based on effic...

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

中国甲壳动物学会第十一届年会暨学术研讨会

作者： Zhi-Huan Xu~*,Gui-Zhong Wang,Qian Mu,li-Sheng Wu,shao-jing li College of Oceanography and Environmental Science,State Key Laboratory of Marine Environmental Science,Xiamen University,Xiamen 361005,China

＜正＞A method of extracting DNA from individual copepod eggs was introduced for this study which included a modified proteinase K procedure and an efficient DNA sedimentation *** was extracted from egg samples including freshly spawned eggs from the three copepod species Apocyclops borneoensis,Centropages tenuiremis and Calanus sinicus,together with eighteen resting eggs separated

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong jing-jing Hu Yan Yu Xiao-li li Xiao-Ting Sun li-Ting Wang Xia Wu ling-Jie Zhu Xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

Highly conductive and stable iodine doped argyrodite electrolyte for all-solid-state lithium batteries

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Journal of Energy Chemistry 2025年第1期100卷 50-58页

作者： Gaozhan liu jing Zhang jing Yang jinghui Chen Xingyue Xiao Xiayin Yao Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences

lithium argyrodites with high ionic conductivity and low cost are considered as one of the most prospective solid electrolytes for all-solid-state lithium ***,the poor chemical stability and compatibility with lithium metal limit their ***,li5.4PS4.4Cl1.4I0.2solid electrolyte with high ionic conductivity of 11.49 m S cm-1and improved chemical stability is synthesized by iodine *** ultra-thin li5.4PS4.4Cl1.4I0.2membrane with thickness of 10μm can be obtained by wet coating process,exhibiting a high ionic conductivity of 2.09 mS cm-1and low areal resistance of 1.17Ω***,iodine doping could in-situ form liI at the lithium/electrolyte interface and improve the critical current density of li5.4PS4.4Cl1.6from 0.8 to 1.35 mA *** resultant liCoO2/li5.4PS4.4Cl1.4I0.2/li battery shows excellent cycling stability at 1 C,with a reversible specific capacity of 110.1 mA h g-1and a retention of 80.5% after 1000 *** addition,the assembled liCoO2/li5.4PS4.4Cl1.4I0.2membrane/li pouch cell delivers an initial discharge capacity of 110.4 mA h g-1and 80.5% capacity retention after 100 cycles.

关键词： Argyrodite electrolytes Iodine doping Ionic conductivity Stability All-solid-state lithium battery