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A feasible,economical,and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways

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Chinese Journal of Natural Medicines 2017年第4期15卷 301-309页

作者： ZHANG Yi-Bei DA Juan ZHANG Jing-Xian li shang-rong CHEN Xin LONG Hua-li WANG Qiu-rong CAI Lu-Ying YAO Shuai HOU Jin-Jun WU Wan-Ying GUO De-An shanghai Research Center for Traditional Chinese Medicine and National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Aconiti Lateralis Radix Praeparata(Fuzi) is a commonly used traditional Chinese medicine in clinic for its potency in restoring yang and rescuing from collapse. Aconiti alkaloids, mainly including monoester-diterpenoidaconitines(MDAs) and diester-diterpenoidaconitines(DDAs), are considered to act as both bioactive and toxic constituents. In the present study, a feasible, economical, and accurate HPLC method for simultaneous determination of six alkaloid markers using the Single Standard for Determination of Multi-Components(SSDMC) method was developed and fully validated. Benzoylmesaconine was used as the unique reference standard. This method was proven as accurate(recovery varying between 97.5%-101.8%, RSD linear(R > 0.999 9) over the concentration ranges, and subsequently applied to quantitative evaluation of 62 batches of samples, among which 45 batches were from good manufacturing practice(GMP) facilities and 17 batches from the drug market. The contents were then analyzed by principal component analysis(PCA) and homogeneity test. The present study provided valuable information for improving the quality standard of Aconiti Lateralis Radix Praeparata. The developed method also has the potential in analysis of other Aconitum species, such as Aconitum carmichaelii(prepared parent root) and Aconitum kusnezoffii(prepared root).

关键词： Aconiti Lateralis Radix Praeparata Single Standard for Determination of Multi-Components (SSDMC) Monoester-literpenoidaconitines (MDAs) Diester-diterpenoidaconitines (DDAs)

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Acupoint Injection Decreases Anesthetic Cosumption during Combined Spinal-Epidural and Patient-Controlled Epidural Labor Analgesia

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Chinese Journal of Integrative Medicine 2022年第3期28卷 257-262页

作者： HUANG Min-li FANG Chang-ping ZHAO Hai-yan ZHANG Zi-jing WU Shu-zhen YI Wei li shang-rong WU ling-ling Department of Obstetrics and Gyn ecology The Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou(510630)China Medical College of Acupuncture-Moxibustion and Rehabilitation Guangzhou University of Chinese MedicineGuangzhou(510006)China Department of Anesthesiology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou(510630)China

Objective:To explore if acupoint injection can improve analgesic effects or delivery outcomes in parturients who received combined spinal-epidural analgesia(CSEA)and patient-controlled epidural analgesia(PCEA)for labor ***:A total of 307 participants were prospectively collected from July 2017 to December *** participants were randomized into the combined acupoint injection with CSEA plus PCEA group(AICP group,n=168)and CSEA plus PCEA group(CP group,n=139)for labor analgesia using a random number *** groups received CSEA plus PCEA at cervical dilation 3 cm during labor process,and parturients of the AICP group were implemented acupoint injection for which bilateral acupoint of Zusanli(ST 36)and Sanyinjiao(SP 6)were selected in *** primary outcome was Visual Analogue Scale(VAS)score,and the secondary outcomes were obstetric outcomes and requirement of anesthetics *** evaluations were performed after ***:The VAS scores were significantly lower in the AICP group than in the CP group at 10,30,60,and 120 min after labor analgesia(all Plity,and reduce adverse reactions in the parturients.(Registration ***-2000003120)

关键词： acupoint injection combined spinal-epidural analgesia labor analgesia anesthetic consumption multimodal analgesia

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Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro

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World Journal of Gastroenterology 2003年第10期9卷 2174-2177页

作者： Yu li Peng shang Ai-rong Qian li Wang Yong Yang Zhi-Nan Chen, Department of Cell Biology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Department of Cell Biology Fourth Military Medical University Xi'an 710032 Shaanxi Province China.

AIM: To study the inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma (HCC) cells in ***: Antisense RNA of HAb18G/CD147 vector PCIasHAb18G was constructed by reversely inserting HAb18G/CD147 cDNA to eukaryotic expression vector PCI-neo. The HCC cell line HHCC was transfected by PCI-asHAb18G via cation liposome. Expression of HAb18G/CD147 of transfected cells selected by G418 (geneticin) was observed by immunohistochemical SP staining and FACS (fluorescence activated cell sorting). Gelatin zymography was used to determine the effect of PCI-asHAb18G on reducing secretions of MMP2 and MMP-9 of the transfected cells. Boyden chamber was employed to test the invasion of HCC cells in ***: The construction of antisense RNA vector PCIasHAb18G was verified correct by partial nucleotide sequencing and restricted endonuclease digestion. The expression of HAb18G/CD147 in transfected HHCC was inhibited by PCI-asHAb18G. Secretions of MMP-2 and MMP9 of transfected HHCC were reduced and the invasion of transfected HHCC was inhibited compared to HHCC,***: Invasion of HCC cells can be inhibited by antisense RNA of HAb18G/CD147. HAb18G/CD147 may be used as a potential target of drugs for anti-invasion and metastasis of HCC.

关键词：肝细胞癌肿瘤侵袭抑制效应 HAb18G/CD147 体外试验

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Effect of Astragalus membranaceus injection on the activity of the intestinal mucosal mast cells after hemorrhagic shock-reperfusion in rats

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Chinese Medical Journal 2006年第22期119卷 1892-1898页

作者： GAN Xiao-liang HEI Zi-qing Huang He-qing CHEN li-xin li shang-rong CAI Jun Department of Anesthesiology Third Affiliated Hospital SunYat-sen University Guangzhou 510630 China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510080 China

Background The mechanism of mucosal damage induced by ischemia-reperfusion （IR） after hemorrhagic shock is complex; mast cells （MC） degranulation is associated with the mucosal damage. Astragalus membranaceus can protect intestinal mucosa against intestinal oxidative damage after hemorrhagic shock, and some antioxidant agents could prevent MC against degranulation. This study aimed to observe the effects of astragalus membranaceus injection on the activity of intestinal mucosal mast cells （IMMC） after hemorrhage shock-reperfusion in rats Methods Thirty-two Wistar rats were randomly divided into the normal group, model group, low dosage group, （treated with Astragalus membranacaus injection, 10 g crude medication/kg） and high dosage group （treated with Astragalus membranacaus injection, 20 g crude medication/kg）. The rat model of hemorrhagic shock-reperfusion was induced by hemorrhage for 60 minutes followed by 90 minutes of reperfusion. The animals were administrated with 3 ml of the test drug solution before reperfusion. At the end of study, intestinal pathology, ultrastructure of IMMC, and expression of tryptase were assayed. The levels of malondisldehyde （MDA）, TNF-α, histamine, and superoxide dismutase （SOD） activity in intestine were detected, and the number of IMMC was counted. Results The Chiu＇s score of the rats in the model group was higher than in other groups （P〈0.01）. The Chiu＇s score in the high dosage group was higher than that in the low dosage group （P〈0.05）. Hemorrhage-reperfusion induced IMMC degranulation： Astragalus membranaceus injection attenuated this degranulation. Expression of tryptase and the number of IMMC in the model group increased compared with the other groups （P〈0.01） and was significantly reduced by the treatments of Astragalus membranaceus injection at both doses. There was no significant difference between the two treatment groups （P〉0.05）. MDA content and concentration of TNF-α in the model group were higher than that in the oth

关键词： Astragalus membranaceus injection hemorrhage shock reperfusion intestinal mucosal mast cells histamine tumor necrosis factor-α

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Relationship between Toll-like receptor 2 on polymorphonuclear neutrophil and postoperative systemic inflammatory response syndrome in patients undergoing orthotopic liver transplantation

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中华麻醉学杂志 2010年第B2期30卷 15-18页

作者： CHI Xin-jin li shang-rong CHENG Nan HEI Zi-qing LUO Gang-jian GUAN Jian-qiang ZHANG Rui ZHANG Qi Department of Anesthesiology Third Affiliated Hospital SUN Yat-Sen University Guangzhou 510630 China

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Perioperative changes of ventricular function and three indicators of myocardial injury during orthotopic liver transplantation

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Chinese Medical Journal 2006年第11期119卷 939-943页

作者： HEI Zi-qing liU De-zhao LUO Chen-fang li shang-rong MA Wu-hua LUO Gang-jian Department of Anesthesiology Third Affiliated Hospital SunYat-sen University Guangzhou 510630 China

Patients undergoing orthotopic liver transplantation may develop significant haemodynamic instability, especially during anhepatic phase and immediately after reperfusion of the graft. The haemodynamic instability may be caused directly by myocardial depression due to pathogenic substances released from the liver, or by acute blood loss.^1 Creatine kinase（CK） and its MB fraction （CK-MB） are sensitive and specific indicators to reflect myocardial damage.^2 Cardiac troponin I （cTnl） is a specific and sensitive marker of myocardial necrosis.^3 This study assessed perioperative cardiac function using three indicators （CK,CK-MB,and CTnl） to evaluate perioperative myocardial damage.

关键词： ventricular function orthotopic liver transplantation creatine kinase cardiac troponin I

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Relationship between the expression of Toll-like receptor 2 and 4 in mononuclear cells and postoperative acute lung injury in orthotopic liver transplantation

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Chinese Medical Journal 2009年第8期122卷 895-899页

作者： CHI Xin-jin CAI Jun LUO Chen-fang CHENG Nan HEI Zi-qing li shang-rong LUO Gang-jian Department of Anesthesiology Third Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong 510630 China

Background The aim of this study was to investigate the potential relationship between the dynamic expression of Toll-like receptor 2 and 4 （TLR2/4） in peripheral blood mononuclear cells as well as changes in serum concentration of inflammatory factors and acute lung injury （ALl） in patients after orthotopic liver transplantation （OLT）. Methods The peripheral blood samples of 27 patients （23 men and 4 women with ASA Ⅲ to Ⅳ） who received OLT were collected for measurement of TLR2/4 at T1 （after induction of anesthesia）, T2 （25 minutes after anhepatic phase）, T3 （3 hours after graft reperfusion） and T4 （24 hours after graft reperfusion）. The expression of TLR2/4 in mononuclear cells was measured by flow cytometry. The serum concentrations of tumor necrosis factor （TNF）-α, interleukin （IL）-1β and IL-8 were measured by enzyme-linked immunosorbent assay （EliSA）. Twenty-seven patients were assigned to ALl group （n=-9） and non-ALl group （n=-18） according to the diagnostic criteria of ALl. The expression of TLR2/4 in the ALl group or non-ALl group was analyzed. Results Compared to the non-ALl group, the volumes of blood loss, ascites, total output and transfused red blood cells were higher in the ALl group, and the anhepatic phase lasted longer （P 〈0.05, P 〈0.01）. The expression of TLR2/4 in mononuclear cells increased significantly at T3 and T4, and serum concentrations of TNF-α, IL-1β and IL-8 increased significantly too. There was no significant difference in Child-Turcotte-Pugh （CTP） scores between the ALl group and non-ALl group （P 〉0.05）. The expression of TLR2/4 in mononuclear cells increased significantly at T3 and T4 in the ALl group （P 〈0.05, P〈0.01）. A positive correlation was noted between the expression of TLR4 in mononuclear cells and the serum concentrations of TNF-α, IL-1β （P=0.041, P=0.046） in the ALl group. In the non-ALl group, statistical results showed that the expression level of TLR2/4 in mononuclear cells was not significantly different during the pe

关键词： orthotopic liver transplantation Toll-like receptor 2 Toll-like receptor 4 acute lung injury

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黄姜皂素提取新工艺研究

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高校化学工程学报 2011年第2期25卷 296-301页

作者：潘鹤林陈晨商利容华东理工大学化工学院上海200237

以酒精为溶剂提取黄姜干粉中的皂苷,皂苷经水解、过滤、中和、干燥、石油醚抽提、结晶、重结晶得到纯度较高的黄姜皂素。实验中采用热提取和高压均质提取两工艺。研究了热提取工艺中溶剂用量、料液比、温度、时间和次数等对皂素收率的影... 详细信息

以酒精为溶剂提取黄姜干粉中的皂苷,皂苷经水解、过滤、中和、干燥、石油醚抽提、结晶、重结晶得到纯度较高的黄姜皂素。实验中采用热提取和高压均质提取两工艺。研究了热提取工艺中溶剂用量、料液比、温度、时间和次数等对皂素收率的影响:溶剂为95%(v)的酒精,料液比为5:1,提取温度为60℃,提取3次,提取时间分别为2 h、1 h、0.5 h时,皂素收率可达2.45%。在此基础上,为提高提取效率,也考察了高压均质提取工艺过程,60 MPa压力下均质提取可达到与热提取同等效果。热提取和高压均质提取工艺因预先分离了皂苷、淀粉和纤维素等,因此大大降低了酸解工艺的处理量,使得皂苷酸解耗酸量和废水量较传统工艺大幅下降,且皂素收率有所提高。

关键词：黄姜皂素溶剂热提取高压均质提取

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异丙酚-芬太尼静脉全麻下人工流产术后恶心呕吐危险因素分析

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实用医学杂志 2013年第20期29卷 3309-3311页

作者：黎尚荣王韧沈宁中山大学附属第三医院麻醉科广州市510630

目的:观察异丙酚-芬太尼静脉全麻下人工流产术后恶心呕吐(PONV)的发生情况,分析其相关的危险因素。方法:采用成组设计的病例-对照研究方法。141例接受人工流产术的患者,术前填写相关因素登记表,术中观察并记录麻醉时间、手术时间、麻醉... 详细信息

目的:观察异丙酚-芬太尼静脉全麻下人工流产术后恶心呕吐(PONV)的发生情况,分析其相关的危险因素。方法:采用成组设计的病例-对照研究方法。141例接受人工流产术的患者,术前填写相关因素登记表,术中观察并记录麻醉时间、手术时间、麻醉药用量,麻醉苏醒期及术后24 h追踪记录PONV发生情况。PONV定义为苏醒期至术后24 h内发生恶心、干呕或呕吐。对各种因素进行单因素及多因素分析,多因素分析采用Logistic(Forward法)逐步回归。结果:54例患者发生了PONV,发病率为38.3%。单因素分析显示麻醉时间、手术时间、丙泊酚用量是PONV的危险因素(均P<0.05),Logistic逐步回归分析并未能进一步筛选出独立的危险因素。结论:异丙酚-芬太尼静脉全麻下人工流产术后PONV的发病率为38.3%,麻醉时间、手术时间、丙泊酚用量可能是PONV发生的危险因素。

关键词：人工流产术全凭静脉麻醉术后恶心呕吐危险因素

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重型肝炎和肝癌肝硬化患者肝移植围手术期肾功能的变化

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中国危重病急救医学 2007年第7期19卷 386-389页

作者：李晓芸黑子清黎尚荣沈宁中山大学附属第三医院麻醉科广东广州510630

目的观察重型肝炎及肝癌肝硬化患者原位肝移植围手术期肾功能变化,评价肝移植术对该类患者肾功能的影响。方法选择30例术前血肌酐（SCr）、尿素氮（BUN）正常的终末期肝病、行背驮式原位肝移植术患者,手术过程中均未采用体外静-静脉... 详细信息

目的观察重型肝炎及肝癌肝硬化患者原位肝移植围手术期肾功能变化,评价肝移植术对该类患者肾功能的影响。方法选择30例术前血肌酐（SCr）、尿素氮（BUN）正常的终末期肝病、行背驮式原位肝移植术患者,手术过程中均未采用体外静-静脉转流。按原发病不同分为重型肝炎组（15例）和肝癌肝硬化组（肝癌组15例）,分别于术前（麻醉后）、无肝前20min、无肝30min、新肝60min、术毕不同时间点取桡动脉血,测定β2-微球蛋白（β2-MG）值,并记录血流动力学变化;分别于术前、新肝60min、术毕留取新鲜尿液,测定尿β2-MG及尿N-乙酰-β-D-葡萄糖苷酶（NAG）值。记录两组患者术前、术后24h、术后1周的SCr、BUN值以及术后肝移植相关性肾功能衰竭（肾衰）的发生情况。结果1术前重型肝炎组有7例血β2-MG、12例尿β2-MG、14例尿NAG值均高于正常参考值;而肝癌组仅有3例血β2-MG、3例尿β2-MG、7例尿NAG值高于正常参考值;两组各时间点血β2-MG异常率比较差异均无显著性（P均〉0.05）;重型肝炎组术前尿β2-MG及NAG异常率明显高于肝癌组（P〈0.01和P〈0.05）。2术中两组血β2-MG值与术前比较变化不大,变化趋势两时间点间比较差异均无显著性;两组新肝期60min及术毕尿β2-MG及NAG均较术前呈增高趋势,但差异无显著性（P均〉0.05）,而重型肝炎组各时间点尿NAG值明显高于肝癌组（P〈0.05或P〈0.01）。3重型肝炎组肝移植相关性肾衰发生率为46.7%,而肝癌组无一例发生（P〈0.01）。结论重型肝炎患者较肝癌肝硬化患者肝移植术后早期易发生急性肾衰,围手术期应注意肾功能保护。

关键词：肝移植肝炎重型肝癌肾功能衰竭,急性围手术期肾功能

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