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Chemical Research in Chinese Universities 2011年第6期27卷 944-948页

作者： GAO Qian WANG Zhi-zeng WANG Qian li shan-shan li yu Research Academy of Energy and Environmental Studies North China Electric Power University Beijing 102206 P. R. China

Artificial neural network（ANN） and full factorial design assisted atrazine（AT） multiple regression adsorption model（AT-MRAM） were developed to analyze the adsorption capability of the main components in the surficial sediments（SSs）. Artificial neural network was used to build a model（the determination coefficient square r2 is 0.9977） to describe the process of atrazine adsorption onto SSs, and then to predict responses of the full factorial design. Based on the results of the full factorial design, the interactions of the main components in SSs on AT adsorption were investigated through the analysis of variance（ANOVA）, F-test and t-test. The adsorption capability of the main components in SSs for AT was calculated via a multiple regression adsorption model（MRAM）. The results show that the greatest contribution to the adsorption of AT on a molar basis was attributed to Fe/Mn（–1.993 μmol/mol）. Organic materials（OMs） and Fe oxides in SSs are the important adsorption sites for AT, and the adsorption capabilities are 1.944 and 0.418 μmol/mol, respectively. The interaction among the non-residual components（Fe, Mn oxides and OMs） in SSs interferes in the adsorption of AT that shouldn’t be neglected, revealing the significant contribution of the interaction among non-residual components to controlling the behavior of AT in aquatic environments.

关键词： Back propagation(BP) artificial neural network Full factorial design Fe/Mn oxide Organic material Atrazine Interaction

Easy-to-use Method to Display Arabidopsis Rubisco Interacting Proteins

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Chemical Research in Chinese Universities 2010年第3期26卷 427-430页

作者： SUN li-wen li Jian-hua li shan-yu XI Jing-hui Key Lab for Molecular Enzymology and Engineering Ministry of Education Jilin University Changchun 130021 P. R. China Department of Engineering Chemistry Mudanjiang University Mudanjiang 157011 P. R. China Department of Pediatrics The First Hospital of Jilin University Changehun 130021 P. R. China College of Plant Science Jilin University Changehun 130062 P. R. China

Affinity-mediated protein separation is an integral part of proteomics, the most outstanding of which is immunoproteomics. However, in the immunoprecipitate system overwhelming Ab and Ag conceal Ag interacting proteins as the research targets, which is the rate-limiting step in the progress of comparative proteomic analyses. We presented a convenient and accurate method to tackle this problem. 1 mol/L NaCl elution buffer was applied to the complex Rubisco immunoprecipitate of Arabidopsis, the weakest force involved in the system was selectively broken up, resulting in the enrichment of Rubisco interacting proteins accessible for further comparative protein gel profile. The easy-to-use method sheds light on a narrow-down strategy supplement for comparative immunoproteomics.

关键词： Comparative proteomics Arabidopsis Rubisco Immunoprecipitation

Chemical Modification of Tryptophan Residues in Superoxide Dismutase from Camellia Pollen and Its Fluorescence Spectrum

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Chemical Research in Chinese Universities 2005年第5期21卷 562-565页

作者： HE Xiao-hong WU Min li shan-yu CHU yu-zhuo CHEN Jia liU Lan-ying College of life Science Jilin University Changchun 130023 P. R. China

The amino acid composition of the superoxide dismutase（SOD） from camellia pollen was measured and the tryptophan（Trp） residues were modified by using N-bromosuccinimide（NBS）. The results show that there are 21 Trp residues in an SOD molecule and seven of which are located on the surface of the enzyme. By researching the fluorescence spectra of the native SOD and the modified SOD, we have found that the emission wavelength of Trp is at 335 nm and the fluorescence intensity will decrease when the enzyme is modified. The results also show that potassium iodide（KI） can significantly quench the fluorescence of the native SOD, but it has a less pronounced effect on the modified enzyme. Glycerin as a surface activation reagent can stabilize the fluorescence of the modified enzyme.

关键词： Superoxide dismutase Tryptophan residue Chemical modification Fluorescence spectrum

Purification and Characterization of Superoxide Dismutase(SOD) from Camellia Pollen

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Chemical Research in Chinese Universities 2005年第5期21卷 558-561页

作者： HE Xiao-hong WU Min li shan-yu FAN Hao CHU yu-zhuo liU Lan-ying College of life Science Jilin University Changchun 130023 P. R. China

A superoxide dismutase（ SOD ） was purified to homogeneity from fresh camellia pollen by means of ammonium sulfate precipitation and column chromatography with DEAE-cellulose（ DE52 ）, Sephadex G-100 and phenyl sepharose^TM 6 Fast Flow columns. Its specific activity could reach to 4034 U/mg protein and it was determined to be Cu/ Zn-SOD according to its different sensitivities to different inhibitors. The molecular weight of the SOD and its subunit were 69500 and 34700, respectively, based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS- PAGE）, which implicates that the SOD in camellia pollen is a dimmer composed of two identical subunits. The isoelectric point of the enzyme was determined to be 4. 1 by isoelectric focusing electrophoresis and the N-terminal amino acid was identified to be Gly by the DNS-Cl method. Its α-Helix was also calculated to be approximately 21.8% according to the circular dichroism（CD） spectra.

关键词： Camellia pollen Superoxide dismutase Purification Characterization

Proteomic Analysis of Differentially Expressed Proteins of Arabidopsis thaliana Response to Specialist Herbivore Plutella xylostella

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Chemical Research in Chinese Universities 2010年第6期26卷 958-963页

作者： liU lin-lin ZHANG Jing ZHANG yun-feng li Yan-chun XI Jing-hui li shan-yu College of Plant Science Jilin University Changchun 130062 P. R. China Department of Pediatrics The First Hospital of Jilin University Changchun 130021 P. R. China Wine Food and Molecular Biosciences Lincoln University Canterbury New Zealand

The changes of the proteome in Arabidopsis thaliana leaves were examined by specialist Plutella *** of about 1100 protein spots on each 2DE gel revealed 38 differentially expressed protein spots in abun-dance of which 34 proteins were identified by MALDI-TOF/TOF *** the insect feeding responsive proteins,a few proteins involved in carbon metabolism were identified including proteins associated with the Calvin cycle in the chloroplast and TCA cycle in the mitochondria,indicating carbon metabolism related proteins may play crucial roles in induced defense response in plants under insect *** analysis elucidates the subcellular location of proteins demonstrates that about 50% of proteins are in the chloroplast,which shows the chloroplast has a key role in the insect feeding response for *** expression analysis of 10 different proteins by quantitative real-time PCR shows that four proteins of the mRNA level were correlated well with the protein *** study further dissected the nature of insect infestation as a stress signal and some novel insect feeding responsive proteins identified may play an important role in induced defence machanism for plant.

关键词： Arabidopsis thaliana Plutella xylostella Proteome Plant-insect interaction

On Network Security Situation Prediction Based on RBF Neural Network 36

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On Network Security Situation Prediction Based on RBF Neural...

A Gene-Pool Based Genetic Algorithm for TSP

第36届中国控制会议

作者： Yang Jiang Cheng-hai li li-shan yu Bo Bao College of Air and Missile Defense Air Force Engineering University

ISBN: (纸本)9781538629185

Aiming at the problem of network security situation prediction,this paper studies the prediction method based on RBF neural *** training the RBF neural network,find out the nonlinear mapping relationship between the front N data and the subsequent Mdata,and then adjust the value of N to explore the different prediction *** simulation result shows that the proposed method can accurately predict the results of the *** with the prediction based on BP neural network,the proposed method has a more accurate prediction and a faster convergence speed,a better prediction effect achieved.

关键词： Network security Situation prediction RBF

来源： cnki会议评论

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Wuhan University Journal of Natural Sciences 2003年第S1期8卷 217-223页

作者： Yang Hui, Kang li-shan, Chen yu-pingState Key Laboratory of Software Engineering, Wuhan University, Wuhan 430072, Hubei, China State Key Laboratory of Software Engineering Wuhan University Wuhan Hubei China

Based on the analysis of previous genetic algorithms (GAs) for TSP, a novel method called Ge- GA is proposed. It combines gene pool and GA so as to direct the evolution of the whole population. The core of Ge- GA is the construction of gene pool and how to apply it to GA. Different from standard GAs, Ge- GA aims to enhance the ability of exploration and exploitation by incorporating global search with local search. On one hand a local search called Ge- Lo-calSearch operator is proposed to improve the solution quality, on the other hand the modified Inver-Over operator called Ge InverOver is considered as a global search mechanism to expand solution space of local minimal. Both of these operators are based on the gene pool. Our algorithm is applied to 11 well-known traveling salesman problems whose numbers of cities are from 70 to 1577 cities. The experiments results indicate that Ge- GA has great robustness for TSP. For each test instance, the average value of solution quality, found in accepted time, stays within 0. 001% from the optimum.

关键词： Genetic Algorithm Gene Pool minimal spanning tree combinatorial optimization TSP

Some Massively Parallel Algorithms from Nature

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Wuhan University Journal of Natural Sciences 2002年第1期7卷 37-46页

作者： li Yan, Kang li-shan, Chen yu-ping, liu Pu, Cao Hong-qing, Pan Zheng-jun The State Key Laboratory of Software Engineering, Wuhan University, Wuhan 430072, Hubei, China The State Key Laboratory of Software Engineering Wuhan University Wuhan Hubei China

We introduced the work on parallel problem solvers from physics and biology being developed by the research team at the State Key Laboratory of Software Engineering, Wuhan University. Results on parallel solvers include the following areas: Evolutionary algorithms based on imitating the evolution processes of nature for parallel problem solving, especially for parallel optimization and model-building; Asynchronous parallel algorithms based on domain decomposition which are inspired by physical analogies such as elastic relaxation process and annealing process, for scientific computations, especially for solving nonlinear mathematical physics problems. All these algorithms have the following common characteristics: inherent parallelism, self-adaptation and self-organization, because the basic ideas of these solvers are from imitating the natural evolutionary processes.

关键词： evolutionary computation parallel algorithm imitating nature domain decomposition knowledge discovery in databases

Quantum interference of multi-orbital effects in high-harmonic spectra from aligned carbon dioxide and nitrous oxide

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Chinese Physics B 2019年第9期28卷 27-31页

作者： Hong-Jing liang Xin Fan Shuang Feng li-yu shan Qing-Hua Gao Bo Yan Ri Ma Hai-Feng Xu Institute of Atomic and Molecular Physics Jilin University

We investigate experimentally multi-orbital effects in high-order harmonic generation(HHG) from aligned CO2 and N2O molecules by intense femtosecond laser fields with linear and elliptical *** either of the aligned molecules, a minimum in the harmonic spectrum is observed, the position of which shifts to lower-order harmonics when decreasing the intensity or increasing the ellipticity of the driving *** indicates that the minimum originates from the dynamic interference of different channels, of which the tunneling ionization and recombination are contributed via different molecular *** results show that both the highest occupied molecular orbital(HOMO) and low-lying HOMO-2 in CO2(or HOMO-1 in N2O) contribute to the molecular HHG in both linearly and elliptically polarized strong laser *** study would pave a way for understanding multi-electron dynamics from polyatomic molecules irradiated by strong laser fields.

关键词： high-order harmonic generation aligned molecule multi-orbital effect dynamic interference

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二维时空熵运动检测中的自适应门限技术

西安电子科技大学学报 2007年第4期34卷 647-653页

作者：刘洋李玉山西安电子科技大学电路CAD研究所陕西西安710071

针对图像序列中运动检测门限的选取问题,提出一种基于二维时空熵准则自适应确定检测门限的新方法.将运动检测归结为两个二值划分问题,无需已知背景分布的具体形式和参数,利用二维熵准则自适应确定门限矢量(S,T).并提出一种二维熵门限快... 详细信息

针对图像序列中运动检测门限的选取问题,提出一种基于二维时空熵准则自适应确定检测门限的新方法.将运动检测归结为两个二值划分问题,无需已知背景分布的具体形式和参数,利用二维熵准则自适应确定门限矢量(S,T).并提出一种二维熵门限快速计算方法,利用积分和迭代操作避免二维熵门限求解过程中点的重复计算,将灰度级为N的图像的运算量从O(N4)降低到O(N2).实验结果表明,该方法在目标和背景对比度较低的情况下也可提取出完整的运动目标信息,可实现实时处理.

关键词：二维时空熵准则运动检测门限运动目标提取积分操作