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[Recent progress in mass spectrometry imaging using nanospray desorption electrospray ionization]

Se pu = Chinese journal of chromatography 2025年第1期43卷 43-49页

作者： Jing-Bo Wang Xiao-Lan li rui-Xia Fan Ping Lü rui-Chuan Yin West China School of Pharmacy Sichuan University Chengdu 610041 China.

Ambient mass spectrometry imaging (MSI) enables hundreds of analytes in tissue sections to be directly mapped at atmospheric pressure with minimal sample preparation. This field is currently experiencing rapid growth, with numerous reported ambient ionization techniques resulting in a "hundred flowers bloom" situation. Nanospray desorption electrospray ionization (nano-DESI), developed by the Laskin group in 2010, is a widely used liquid-extraction-based ambient ionization technique that was first used for mass spectrometry imaging of tissue in 2012. The nano-DESI probe comprises a primary capillary and a nanospray capillary, with the latter efficiently transferring analyte-containing droplets via a tiny liquid bridge formed between the probe and sample surface, thereby enabling nanoelectrospray ionization (nano-ESI) in front of the inlet of a mass spectrometer. The advantages of nano-DESI MSI include minimal sample preparation, high spatial resolution, and high sensitivity. These features are well-suited for imaging various sample types, including frozen tissue sections, microbial communities, and environmental samples. A PubMed-database search using the "nano-DESI" keyword revealed 72 related articles in the 2010-2024 period, with 34 of them published between 2021 and 2024, which indicates that nano-DESI has rapidly developed as an ambient ionization technique over recent years. Herein, we briefly introduce key nano-DESI-MSI research progress reported in the past three years with the aim of better understanding and facilitating the use of this technology. We first discuss advances in ion-source development. Since no commercial nano-DESI source exists, designing and constructing ion sources remain technical challenges that limit its development. Nano-DESI has been successfully coupled with various types of mass spectrometer, including LTQ Orbitrap, quadrupole-Orbitrap (Q Exactive), 6560 IM QTOF, timsTOF Pro2, triple quadrupole, and FTICR. These couplings have signi

关键词： ambient mass spectrometry imaging nanospray desorption electrospray ionization spatial proteomics structural characterization of lipid

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Experimental Study on Diesel Spray and Flame Asymmetry Characteristics of Dual-Orifice Nozzles for a Medium-Speed Engine

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Journal of Thermal Science 2025年第1期 303-322页

作者： JU Dehao liU rui zheng liang DENG Jiahui ZHANG Wenzheng HUO Jinlu HUANG li Shanghai Marine Diesel Engine Research Institute National Key Laboratory of Marine Engine Science and Technology Key Laboratory for Power Machinery and Engineering Ministry of EducationShanghai Jiao Tong University

The asymmetry of the multi-orifice spray will cause uneven heat load of the marine diesel engine,thereby affecting its working performance and service ***,an in-depth understanding of the spray and flame characteristics of multi-orifice nozzles will guide the optimization of the nozzle structure,needle design and diesel atomization and combustion *** this reason,four groups of dual-orifice nozzles with different hole diameters(0.1-0.55 mm) and mass flow rates covering the typical marine medium-speed diesel injections are designed and customized,and the constant volume chamber(CVC) with high temperature and pressure is used to simulate the actual in-cylinder working conditions of the diesel engine for the spray visualization *** study the asymmetry of the fuel sprays discharged from a diesel injector,the multi-orifice nozzle is simplified as a dual-orifice nozzle in this *** with X-ray Computed Tomography(CT) imaging technology,the influences of the nozzle internal structure on the spray and flame asymmetry are studied in the constructed supercritical *** is found that the asymmetry of the inlet angle and the equivalent length-diameter ratio is positively correlated with the inconsistency of the dual *** an increase in the injection pressure and nozzle diameter,the asymmetry of the dual spray becomes more pronounced,resulting in greater disparities in the ignition delay times and ignition positions of the two ***,the increase in nozzle diameter also leads to combustion instability,resulting in a flame with a serrated *** the increase of ambient temperature,the proportion of liquid phase in the jet decreases and the relative density of spray front decreases.

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山区公路类土质深路堑高边坡处治

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山西建筑 2010年第21期36卷 296-298页

作者：赖增成黎锐峥中国公路工程咨询集团有限公司

结合福建东部沿海山区高速公路类土质深路堑高边坡特点,分别介绍了类土质深挖路堑高边坡的处治办法,对工程实践中出现的滑坡现象进行了分析,论述了设计施工中注意的重要方面,对今后相关深路堑高边坡设计与施工有一定的借鉴意义。

关键词：高边坡深路堑滑坡类土质公路

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基于遥感影像不同亮度的CART方法估算不透水率研究

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水电能源科学 2010年第12期28卷 45-48页

作者：李倩李彩丽芮菡艺郑大鹏都金康南京大学地理信息科学系江苏南京210093

以QuickBird影像为辅助数据,基于中巴资源卫星影像研究了不透水率估算方法,改进了传统的基于CART的估算方法,并将中巴资源卫星影像不透水面像元分类,对不同亮度分别使用CART方法估算不透水率。试验结果表明,该方法构建的模型简单合理,... 详细信息

以QuickBird影像为辅助数据,基于中巴资源卫星影像研究了不透水率估算方法,改进了传统的基于CART的估算方法,并将中巴资源卫星影像不透水面像元分类,对不同亮度分别使用CART方法估算不透水率。试验结果表明,该方法构建的模型简单合理,运算效率高、精度高,可供借鉴。

关键词：遥感影像不同亮度分类与回归树估算不透水率

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Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu Xiangbing Wu Wei Wu Ying Wang Yan Sun lingxiao Deng Melissa Walker Chen Chen Heqiao Dai Qi Han Ying Ding Yongzhi Xia George Smith rui li Nai-Kui liu Xiao-Ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

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有为政府与有效市场下的我国职业教育治理:理论逻辑与实践路径

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高校教育管理 2025年第1期19卷 62-73页

作者：李政邱懿华东师范大学职业教育与成人教育研究所上海200062 中华人民共和国教育部职业教育与成人教育司北京100816 北京大学教育学院北京100871

政府与市场的关系是职业教育内外部治理关系的核心,先后经历了政府主导论、市场基础论、有为政府与有效市场论的演变。在有为政府与有效市场的关系形塑下,职业教育治理改革应实现三大转变,即政府治理方式应从事后响应转变为事前布局,职... 详细信息

政府与市场的关系是职业教育内外部治理关系的核心,先后经历了政府主导论、市场基础论、有为政府与有效市场论的演变。在有为政府与有效市场的关系形塑下,职业教育治理改革应实现三大转变,即政府治理方式应从事后响应转变为事前布局,职业院校治理角色应从代理主体转变为改革先锋,市场主体参与治理的动机应从短期利益转变为战略投资。政府、职业院校和市场的关系应由单向的“影响回应”关系转变为双向的“并行互动”关系。当前,我国政府参与职业教育治理的权责边界不清,职业院校办学自主权无法满足发展需求,市场主体参与职业教育缺乏战略自觉。为此,我国应加强各级政府的政策协作,提升政府政策的精准度和前瞻性;优化职业院校组织定位和内部治理模式,提升学校治理的灵活性和有效性;深度分析企业发展的外部条件和内部需求,提升企业发展的积极性和适应性。

关键词：职业教育治理有为政府有效市场全面深化改革办学自主权

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基于Icepak的航天电子产品散热优化仿真分析

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电子机械工程 2019年第2期35卷 38-42页

作者：李正睿杨欣悦吴静湖北三江航天红峰控制有限公司湖北孝感432000

随着航天电子产品朝着小型化和集成化方向发展,电子产品内部电子元器件的集成度和功率日益提高。传统的热设计主要基于实物试验采集机箱内部印制板核心元器件的温度,再根据实际情况进行印制板布局调整或结构件改进设计,该过程周期长且... 详细信息

随着航天电子产品朝着小型化和集成化方向发展,电子产品内部电子元器件的集成度和功率日益提高。传统的热设计主要基于实物试验采集机箱内部印制板核心元器件的温度,再根据实际情况进行印制板布局调整或结构件改进设计,该过程周期长且需要投入大量的实物试验成本和时间成本,严重制约了航天型号的研制周期。因此,文中以一种航天电子产品为例,通过运用Icepak软件对机箱内部元器件散热问题进行仿真,并通过优化改进,实现了对元器件温度降低达10℃以上,提升了产品可靠性。这种仿真手段相比传统模式大大缩短了航天产品的研发周期,降低了研发成本。

关键词： Icepak 散热优化航天电子产品

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Unlocking the future:Mitochondrial genes and neural networks in predicting ovarian cancer prognosis and immunotherapy response

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World Journal of Clinical Oncology 2025年第1期16卷 43-52页

作者： Zhi-Jian Tang Yuan-Ming Pan Wei li rui-Qiong Ma Jian-liu Wang Department of Obstetrics and Gynecology Peking University People’s HospitalBeijing 100044China Cancer Research Center Beijing Chest HospitalBeijing 101149China Department of Thoracic Surgery Sichuan Provincial People's HospitalChengdu 610072Sichuan ProvinceChina

BACKGROUND Mitochondrial genes are involved in tumor metabolism in ovarian cancer(OC)and affect immune cell infiltration and treatment *** To predict prognosis and immunotherapy response in patients diagnosed with OC using mitochondrial genes and neural *** Prognosis,immunotherapy efficacy,and next-generation sequencing data of patients with OC were downloaded from The Cancer Genome Atlas and Gene Expression *** genes were sourced from the MitoCarta3.0 *** discovery cohort for model construction was created from 70% of the patients,whereas the remaining 30% constituted the validation *** the expression of mitochondrial genes as the predictor variable and based on neural network algorithm,the overall survival time and immunotherapy efficacy(complete or partial response)of patients were *** In total,375 patients with OC were included to construct the prognostic model,and 26 patients were included to construct the immune efficacy *** average area under the receiver operating characteristic curve of the prognostic model was 0.7268[95% confidence interval(CI):0.7258-0.7278]in the discovery cohort and 0.6475(95%CI:0.6466-0.6484)in the validation *** average area under the receiver operating characteristic curve of the immunotherapy efficacy model was 0.9444(95%CI:0.8333-1.0000)in the discovery cohort and 0.9167(95%CI:0.6667-1.0000)in the validation *** The application of mitochondrial genes and neural networks has the potential to predict prognosis and immunotherapy response in patients with OC,providing valuable insights into personalized treatment strategies.

关键词： Ovarian cancer Mitochondria Prognosis Immunotherapy Neural network

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离子色谱法测定酸雨中阳离子的含量

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陇东学院学报 2016年第5期27卷 36-39页

作者：张小伟李正瑞魏晓霞陇东学院化学化工学院甘肃庆阳745000 兰州市环境监测站甘肃兰州730000

采用离子色谱法测定不同时段酸雨中阳离子的含量,用5mmol/L的甲基磺酸溶液作为淋洗液,分别对酸雨中的Na^+、K^+、Ca^(2+)、Mg^(2+)进行检测,实验结果表明:酸雨样品中的这些阳离子浓度均在一定范围内与其峰面积成线性关系,方法的检出限在... 详细信息

采用离子色谱法测定不同时段酸雨中阳离子的含量,用5mmol/L的甲基磺酸溶液作为淋洗液,分别对酸雨中的Na^+、K^+、Ca^(2+)、Mg^(2+)进行检测,实验结果表明:酸雨样品中的这些阳离子浓度均在一定范围内与其峰面积成线性关系,方法的检出限在0.5~10.2μg/L之间。

关键词：离子色谱法酸雨阳离子测定

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压力性尿失禁诊疗135例临床分析(英文)

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中华临床医师杂志（电子版） 2011年第21期5卷 6336-6341页

作者：李环吴瑞芳李瑞珍张礼婕王苏梅张巍颖 Department of Obstetrics and Gynecology Beijing University shenzhen Hospital

Objective The purpose of this study is to evaluate and compare the two different treatments by researching 135 cases with stress urinary *** From June 2006 to June 2010,135 patients with stress urinary incontinence were treated in Department of Gynecology and Obstetrics of Peking University Shenzhen Hospital.91 cases of them were treated with low frequency electric stimulation and biofeedback therapy (Group A),and the other 44 cases were treated by improved transobturaor tension-free vaginal tape(TVT-O) and TVT-O (Group B).Then,we compared these two groups by whole recovery rate,recovery rate of patients with urge incontinence,charge of treatment and complication,*** Group B's recovery rate for stress urinary incontinence was 100%.Ⅰ degree stress urinary incontinence in Group A's recovery rate was higher;Ⅱ degree patients' recovery rate for stress urinary incontinence was lower;Ⅲ degree patients' recovery rate was 0%.Only 1 case showed abnormal urine stream;2 cases showed urinary retention,but the symptoms disappeared after the *** significant complication in Group *** were huge differences of the expense between the two *** effect in Group A for patients with urge incontinence was good,but there was no effect shown in Group *** The two treatments are safe and effective for stress urinary incontinence,but there are existed prominent differences of the therapeutic efficacy between the two groups.

关键词： Urinary incontinence,stress Urge incontinence Electric stimulation therapy Biofeedback Transobturaor tension-free vaginal tape

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