检索结果-南通市图书馆

微生态活菌制品杂菌检查方法适用性研究及决策方法的建立

在线全文

学校读者我要写书评

暂无评论

药物分析杂志 2024年第8期44卷 1415-1429页

作者：冯丹阳孟晓丽沈振任丽宏丁勃山东省食品药品检验研究院山东省食品药品安全检测工程技术研究中心济南250101 中国药科大学生物药物学院南京211112 山东大学药学院济南250012

目的:根据方法适用性试验结果,对微生态活菌制品的杂菌检查方法进行分类,并建立微生态活菌制品杂菌检查决策方法。方法:按《中华人民共和国药典》(简称《中国药典》)四部(简称要求),以大肠埃希菌、金黄色葡萄球菌、枯草芽孢杆菌、铜绿... 详细信息

目的:根据方法适用性试验结果,对微生态活菌制品的杂菌检查方法进行分类,并建立微生态活菌制品杂菌检查决策方法。方法:按《中华人民共和国药典》(简称《中国药典》)四部(简称要求),以大肠埃希菌、金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌、白色念珠菌和黑曲霉为试验菌,采用胰酪大豆胨琼脂培养基、沙氏葡萄糖琼脂培养基、孟加拉红琼脂培养基、玫瑰红钠琼脂培养基,对已上市微生态活菌制品进行杂菌检查方法适用性试验。结果:成分为厌氧菌或生长特性与试验菌差别较大微生物的制品,可参照《中国药典》四部进行方法适用性试验;成分中含有芽孢杆菌和肠球菌的制品,真菌计数可参照《中国药典》四部进行方法适用性试验;受成分菌的影响,非致病性杂菌无法通过方法适用性试验的产品,可采用选择性培养基对革兰阴性杆菌进行控制;无法通过计数方法适用性试验的制品,可根据给药途径、用药人群等,基于风险评估后,控制部分有潜在危害的微生物。结论:本研究为微生态活菌制品的杂菌检查方法提供了选择依据,进一步补充完善了微生态活菌制品的质量标准体系。

关键词：生物制品微生态活菌制品微生物污染控制杂菌检查方法适用性试验

药品中污染微生物的鉴定和溯源能力验证结果与分析

在线全文

学校读者我要写书评

暂无评论

药物分析杂志 2024年第3期44卷 427-433页

作者：徐晓洁冯丹阳任丽宏孟晓丽沈振邢晟丁勃山东省食品药品检验研究院济南250101 山东省食品药品安全检测工程技术研究中心济南250101 产业技术基础公共服务平台济南250101 山东大学药学院济南250012

目的:通过药品中污染微生物鉴定和溯源分析能力验证,考察山东省药品生产企业对污染微生物的鉴定、溯源以及对微生物异常检测结果的处理分析能力。方法:本次能力验证模拟一起药品微生物污染事件,设计产品污染组(共5个样品,模拟5个产品,其... 详细信息

目的:通过药品中污染微生物鉴定和溯源分析能力验证,考察山东省药品生产企业对污染微生物的鉴定、溯源以及对微生物异常检测结果的处理分析能力。方法:本次能力验证模拟一起药品微生物污染事件,设计产品污染组(共5个样品,模拟5个产品,其中1个加入2种目标菌混合物,其余4个为阴性)和生产环节污染组(共5个样品,模拟5个生产环节,其中4个分别加入了单一目标菌或干扰菌,其余1个为阴性)的能力验证样品,264家药品生产企业参与了能力验证活动,从样品检验,微生物种属鉴定,微生物系统进化分析,污染溯源等方面,评价参加单位的微生物鉴定分析的综合能力。结果:259家参与企业报告了结果。3.5%的企业未能正确判断阴性或阳性样品,判为不合格;49.8%的企业虽正确检测了样品,但未能正确鉴定出微生物种属或未进行微生物鉴定操作,判为合格;46.7%的企业正确鉴定出微生物种属,但未能进行有效的溯源分析,判为良好;仅有4家企业基于16S rRNA基因构建了系统发育树,但未能在菌株水平上进行近缘关系比对和溯源。结论:多数参与企业基本具备检出污染微生物的能力。但是,污染微生物的鉴定、溯源分析能力以及处理异常检测结果的能力还需要进一步加强。

关键词：能力验证微生物鉴定微生物污染溯源亲缘关系比对微生物检验

litterfall,litter Decomposition,and Nutrient Dynamics in Two Subtropical Bamboo Plantations of China

在线全文

学校读者我要写书评

暂无评论

Pedosphere 2014年第1期24卷 84-97页

作者： TU li-Hua HU hong-ling HU Ting-Xing ZHANG Jian li Xian-Wei liU li XIAO Yin-Long CHEN Gang li ren-hong College of Forestry Sichuan Agricultural University Institute of Ecological Forestry College of ForestrySichuan Agricultural UniversityChengdu CampusWenjiang District Personnel Department Sichuan Agricultural University Sichuan Forestry Inventory and Plan Institute

Bamboos are one of the fast-growing and multiple use species in the world, and thus bamboo forests/plantations play an important role in C sequestration at regional and global levels. We studied aboveground litterfall, litter decomposition and nutrient dynamics for two years in two subtropical bamboo ecosystems in Southwest China so as to test the hypothesis that litter quality determine the rate and nutrient dynamics during decomposition of different litter fractions. Mean annual total aboveground litter production ranged from 494 to 434 g m-2 in two bamboo stands （P stand, dominated by Pleioblastus amarus and H stand, hybrid bamboo dominated by Bambusa pervariabilis x Dendrocalamopsis daii）. Bulk （-80%） of litter production was contributed by leaf litter in two stands followed by twigs and sheathes. Different litter fractions represented considerable variations in the rates of mass loss and nutrient release. Variation of the mass remaining after 2 years of decomposition was significantly explained by initial C/N ratio and initial P concentration. Initial concentrations of N, P, Ca, and Mg explained 57.9%, 95.0%, 99.8% and 98.1%, respectively, of the variations of these elements mass remaining after 2 years of decomposition. The patterns of nutrient dynamics and the final amount remaining were mainly determined by their initial litter substrate quality in tl^ese two subtropical bamboo plantations.

关键词： C/N ratio litter fraction litter substrate mass loss Pleioblastus amarus

CdTe quantum dots as fluorescence sensor for the determination of vitamin B6 in aqueous solution

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2008年第7期19卷 855-859页

作者： Jie Fang Sun Cui ling ren li hong liu Xing Guo Chen Department of Chemistry Lanzhou University Lanzhou 730000 China

A novel, rapid and simple CdTe quantum dots （QDs） based technology platform was established for selective and sensitive determination of vitamin B6 in aqueous solution. It can perform accurate and reproducible quant... 详细信息

关键词： CdTe QDs Determination Fluorescence sensor Vitamin B6

Effect of bifidobacterium on defensin-5 expression in intestinal injury of preweaning rats

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第9期21卷 2638-2644页

作者： Wei Wang Shu-Fen Yang li-hong ren Xiu-Xiu Zhang Shui-lian Yu Department of Pediatrics the Second Affiliated Hospital of Harbin Medical University

AIM:To investigate the protective effect of bifidobacterium in endotoxin-induced intestinal injury in preweaning ***:Preweaning rats were randomly divided into three groups(n =40 for each):a control group(groupC),a model group(group E) and a treatment group(group T).Both groups E and T were intraperitoneally injected with lipopolysaccharide(LPS) at a dose of 5 mg/kg(5 mg/L in normal saline),and group T was intragastrically administrated with bifidobacterium suspension(2.0 × 109 CFU/m L,0.5 m L each time,twice a day,until the end of the experiment) 7 d before LPS *** C was intraperitoneally injected with normal *** intraperitoneal injection and intragastric administration,the rats were placed back to the initial cage to receive breast *** rats were killed at 2,6,12,24 or 72 h,respectively,after endotoxin or physiological saline injection to collect serum and ileal tissue ***(MPO) contents in serum and ileum were detected at different times,and expression of ileal defensin-5 m RNA was evaluated by reverse transcription-polymerase chain ***:Serum and ileal MPO contents in group E were significantly higher than those in group C(serum contents:107.50 ± 17.70 vs 157.14 ± 24.67,P rently in 24 *** expression of defensin-5 m RNA at 2 h in group T was significantly higher than that in group C(0.824 ± 0.158 vs

关键词： Bifidobacterium lipopolysaccharide Ileum Myelopero

Decomposition of Different litter Fractions in a Subtropical Bamboo Ecosystem as Affected by Experimental Nitrogen Deposition

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2011年第6期21卷 685-695页

作者： TU li-Hua HU hong-ling HU Ting-Xing ZHANG Jian liU li li ren-hong DAI hong-Zhong LUO Shou-Hua Sichuan Provincial Key Laboratory of Forestry Ecological Engineering College of Forestry Sichuan Agricultural University Xingkang Road 46 Ya'an 625014 (China) Maize Research Institute Sichuan Agricultural University Ya'an 625014 (China) Sichuan Forestry Inventory and Plan Institute Chengdu 610081 (China)

As an important component of the global carbon （C） budget, litter decomposition in terrestrial ecosystems is greatly affected by the increasing nitrogen （N） deposition observed globally. We hypothesized that different litter fractions derived from a single tree species may respond to N deposition differently depending on the quality of the litter substrate. To test the hypothesis, a two-year field experiment was conducted using the litterbag method in a Pleioblastus amarus plantation in the rainy region of Southwest China. Four N treatment levels were applied： control （no N added）, low-N （50 kg N ha-1 year-1）, medium-N （150 kg N ha-a year-1）, and high-N （300 kg N ha-1 year-l）. We observed different patterns of mass loss for the three P. amarus litter fractions （leaves, sheaths, and twigs） of varying substrate quality in the control plots. There were two decomposition stages with different decay rates （fast rate in early stages and slow rate in the later stages） for leaves and sheaths, while we did not observe a slower phase for the decay of twigs during the 2-year study period. The annual decomposition rate （k） of twigs was significantly lower than that of leaves or sheaths. Addition of N slowed the decomposition of leaves and twigs in the later stages of decomposition by inhibiting the decay of lignin and cellulose, while addition of N did not affect the mass loss of sheaths during the study period. In the decomposition of all three litter fractions, experimental N deposition reduced the net N accumulation in the early stages and also decreased the net N release in the later stages. The results of this study suggest that litter substrate quality may be an important factor affecting litter decomposition in a bamboo ecosystem affected by N deposition.

关键词： decomposition rate litter substrate N dynamics plant biomass Pleioblastus amarus

Audio-visual keyword transformer for unconstrained sentence-level keyword spotting

在线全文

学校读者我要写书评

暂无评论

CAAI Transactions on Intelligence Technology 2024年第1期9卷 142-152页

作者： Yidi li Jiale ren Yawei Wang Guoquan Wang Xia li hong liu Key Laboratory of Machine Perception Peking UniversityShenzhen Graduate SchoolShenzhenChina College of Electronics and Information Engineering Sichuan UniversityChengduChina Department of Computer Science ETH ZurichZurichSwitzerland

As one of the most effective methods to improve the accuracy and robustness of speech tasks,the audio-visual fusion approach has recently been introduced into the field of Keyword Spotting(KWS).However,existing audio-visual keyword spotting models are limited to detecting isolated words,while keyword spotting for unconstrained speech is still a challenging *** this end,an Audio-Visual Keyword Transformer(AVKT)network is proposed to spot keywords in unconstrained video *** authors present a transformer classifier with learnable CLS tokens to extract distinctive keyword features from the variable-length audio and visual *** outputs of audio and visual branches are combined in a decision fusion *** humans can easily notice whether a keyword appears in a sentence or not,our AVKT network can detect whether a video clip with a spoken sentence contains a pre-specified ***,the position of the keyword is localised in the attention map without additional position ***-imental results on the LRS2-KWS dataset and our newly collected PKU-KWS dataset show that the accuracy of AVKT exceeded 99%in clean scenes and 85%in extremely noisy *** code is available at https://***/jialeren/AVKT.

关键词： artificial intelligence multimodal approaches natural language processing neural network speech processing

Accelerating lithium-sulfur battery reaction kinetics and inducing 3D deposition of li_(2)S using interactions between Fe_(3)Se_(4)and lithium polysulfides

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2024年第8期95卷 540-553,I0012页

作者： Yihan lin liheng li Longjie Tan Yongliang li Xiangzhong ren Peixin Zhang Chuanxin He lingna Sun College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060GuangdongChina

Although lithium-sulfur batteries(LSBs)exhibit high theoretical energy density,their practical application is hindered by poor conductivity of the sulfur cathode,the shuttle effect,and the irreversible deposition of li_(2)*** address these issues,a novel composite,using electrospinning technology,consisting of Fe_(3)Se_(4)and porous nitrogen-doped carbon nanofibers was designed for the interlayer of *** porous carbon nanofiber structure facilitates the transport of ions and electrons,while the Fe_(3)Se_(4)material adsorbs lithium polysulfides(liPSs)and accelerates its catalytic conversion ***,the Fe_(3)Se_(4)material interacts with soluble liPSs to generate a new polysulfide intermediate,li_(x)FeS_(y)complex,which changes the electrochemical reaction pathway and facilitates the three-dimensional deposition of li_(2)S,enhancing the reversibility of *** designed LSB demonstrates a high specific capacity of1529.6 mA h g^(-1)in the first cycle at 0.2 *** rate performance is also excellent,maintaining an ultra-high specific capacity of 779.7 mA h g^(-1)at a high rate of 8 *** investigation explores the mechanism of the interaction between the interlayer and liPSs,and provides a new strategy to regulate the reaction kinetics and li_(2)S deposition in LSBs.

关键词： lithium-sulfur batteries Polysulfide intermediates li_(2)S electrodeposition Interlayers Electrostatic spinning Adsorption Catalysis

Acaricidal effect of the antimicrobial metabolite xenocoumacin 1 on spider mite control

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第3期23卷 948-959页

作者： Jiaxing Wei hong Yan Jie ren Guangyue li Bo Zhang Xuenong Xu Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory for Biology of Plant Diseases and Insect Pests Beijing 100193China Key Laboratory of Natural Enemies Insects Ministry of Agriculture and Rural AffairsBeijing 100193China

The two-spotted spider mite,Tetranychus urticae Koch,is one of the most harmful pests in many agroecosystems *** effectively manage this pest,there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting *** this study,we explored the acaricidal effects of xenocoumacin 1 (Xcn1) on *** and its predator Neoseiulus californicus using the highly puri?ed ***1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identi?cation (easyPACId) *** the concentration of Xcn1 exceeded 100μg mL~(–1),the survival rate of spider mite adults declined to below 40%and the fecundity was decreased by 80%at six days *** concentrations of 25 and 50μg mL~(–1),Xcn1 signi?cantly impeded spider mite development by inhibiting the ***,neither concentration had any adverse effects on the survival or reproduction of the predatory mite *** results from laboratory and semi-?eld experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological *** study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.

关键词： pest management predatory mite Xcn1 morphology developmental inhibition transcriptome