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Effects of fasting and refeeding on expression of MAFbx and MuRF1 in chick skeletal muscle

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Science China(life Sciences) 2011年第10期54卷 904-907页

作者： li qinghe li JinXiu LAN He WANG Nan HU XiaoXiang CHEN li li Ning State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 China College of Veterinary Medicine China Agricultural University Beijing 100193 China Institute of Animal Sciences and Veterinary Medicine Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

The present study investigated the effects of fasting and refeeding on the expression of proteasorne-related genes and their downstream targets in the skeletal muscles of chicks. Seven-day-old chicks were fasted for 24 or 48 h and then refed for 4 h. The expression levels of MAFbx and MuRF1, which function as E3 ligases in the ubiquitin-proteasome system, were investigated at the mRNA and protein levels. MAFbx and MuRF1 expression were increased by fasting and these increases were downregulated by refeeding. The expression of the target proteins of these E3 ligases, MyoD and M-CK, was also analyzed. The levels of these proteins were downregulated by fasting, and these decreases were rescued by refeeding. The results of this study indicate that fasting stimulates MAFbx and MuRF1 expression in chicks, possibly leading to increased degradation of their corresponding target proteins.

关键词： fasting gene expression MAFbx MuRF1 MyoD M-CK

Dispersion attenuation structure of the crust around the Weifang-Jiashan segment of the Tanlu fault zone in North China

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Earthquake Science 2012年第1期25卷 83-93页

作者： Xiqiang liu qinghe li Hong li Earthquake Administration of Shandong Province Ji'nan 250014China Earthquake Administration of Jiangsu Province Nanjing 210014China

On the condition that the velocity ratio of compressive wave to shear wave is stable during the propagating process of seismic wave, this paper develops an estimation method for medium quality factor based on single station＇s travel time difference between direct S and P waves and the first period signal of direct P wave. 8 774 high SNR wave data altogether recorded by 75 stations are analyzed. The results show that：（1） under the normal regional stress field, the quality factors QmsP in the stable tectonic area are higher than that in the active tectonic area around the Weifang-Jiashan section and its surrounding area in the Tanlu fault zone; （2） in the Juxian-Tancheng section the seismic wave attenuation is relatively quick, and the media is relatively broken, suggesting no tectonic stress accumulation; （3） the Xinyi-Sihong section is currently locking and in accumulating elastic strain energy stage, which has the deep environment and conditions of strong earthquake generation similar to those of the 1668 M8.5 Tancheng strong earthquake.

关键词： dispersion attenuation quality factor measurement method Tanlu fault zone seismicrisk

Chicken gga-miR-1306-5p targets Tollip and plays an important role in host response against Salmonella enteritidis infection

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Journal of Animal Science and Biotechnology 2019年第4期10卷 873-883页

作者： Weiwei Sun Ranran liu Peng li qinghe li Huanxian Cui Maiqing Zheng Jie Wen Guiping Zhao Institute of Animal Sciences Chinese Academy of Agricultural Sciences State Key Laboratory of Animal Nutrition

Background: Increasing evidence indicates that micro RNAs(mi RNAs) are involved in inflammatory response and immune regulation following pathogen invasion. The purpose of this study was to elucidate the roles played by Gallus gallus micro RNA-1306-5 p(gga-mi R-1306-5 p) in host responses against potential invasion by Salmonella enteritidis(SE) in chickens and the underlying ***: In present study, the expression levels of gga-mi R-1306-5 p were determined in both tissues and HD11 cells. The results showed that gga-mi R-1306-5 p was significantly increased following SE infection or lipopolysaccharide(LPS) stimulation. The dual luciferase reporter assay further validated that gga-mi R-1306-5 p targeted the Toll-interacting protein(Tollip), and thereby participated in the regulation of immune response against SE or LPS stimulation through binding with the 3′-untranslated region(3’UTR) of Tollip. Additionally, the expression of Tollip was significantly blocked by over-expressed gga-mi R-1306-5 p. The underlying mechanisms by which ggami R-1306-5 p modulated the production of pro-inflammatory cytokines were also investigated. Molecular biological assays demonstrated that overexpression of gga-mi R-1306-5 p promoted the production of pro-inflammatory mediators, including NF-κB, TNF-α, IL-6, and IL-1β, which produced effects similar to those of Tollip ***: Taken together, gga-mi R-1306-5 p induced by SE or LPS, regulates the immune response by inhibiting Tollip, which activates the production of inflammatory cytokines. This study has provided the first direct evidence that gga-mi R-1306-5 p targets Tollip, and is involved in the host response against SE.

关键词： gga-miR-1306-5p LPS Pro-inflammatory cytokine Salmonella enteritidis Tollip

A significant quantitative trait locus on chromosome Z and its impact on egg production traits in seven maternal lines of meat-type chicken

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Journal of Animal Science and Biotechnology 2023年第1期14卷 47-59页

作者： Jiqiang Ding Fan Ying qinghe li Gaomeng Zhang Jin Zhang Ranran liu Maiqing Zheng Jie Wen Guiping Zhao Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijingChina. MiLe Xinguang Agricultural and Animal Industrials Corporation MiLeChina.

Background:Egg production is economically important in the meat-type chicken *** better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wide association analyses combined with meta-analyses,Bayesian analyses,and selective sweep analyses were performed to screen single nucleotide polymorphisms(SNPs)and other genetic loci that were significantly associated with egg number traits in 11,279 chickens from seven material ***:Yellow-feathered meat-type chickens laid 115 eggs at 43 weeks of age and white-feathered chickens laid 143 eggs at 60 weeks of age,with heritability ranging from 0.034–*** on meta-analyses and selective sweep analyses,one region(10.81–13.05 Mb)on chromosome Z was associated with egg number in all *** analyses using the W2 line was also associated with the same region,and 29 SNPs were identified that significantly affected estimation of breeding value of egg *** 29 SNPs were identified as having a significant effect on the egg number EBV in 3194 birds in line *** are 36 genes in the region,with glial cell derived neurotrophic factor,DAB adaptor protein 2,protein kinase AMP-activated catalytic subunit alpha 1,NAD kinase 2,mitochondrial,WD repeat domain 70,leukemia inhibitory factor receptor alpha,complement C6,and complement C7 identified as being potentially affecting to egg *** addition,three SNPs(rs318154184,rs13769886,and rs313325646)associated with egg number were located on or near the prolactin receptor ***:Our study used genomic information from different chicken lines and populations to identify a genomic region(spanning 2.24 Mb)associated with egg *** genes and 29 SNPs were identified as the most likely candidate genes and variations for egg *** results contribute to the identification of candidate genes and variants for egg traits in poultry.

关键词： Candidate gene Egg production Genome-wide association study Meat-type chicken Selective sweep analysis

Changes in the pattern of DNA methylation between the wild and domestic chicken

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Changes in the pattern of DNA methylation between the wild a...

Characteristics of S-wave Envelope Broadening in the Changbaishan Tianchi Volcano

中国生物化学与分子生物学会第十届会员代表大会暨全国学术...

作者： qinghe li Ning li Jinxiu li Xiaoxiang Hu China Agricultural University

Cytosine DNA methylation is an important epigenetic modification termed as the fifth base that functions in many biological *** modifications may exert their influence on phenotype during the process of selective *** we show the genome-wide DNA methylation map of birds,using the chicken as a model organism and an immunocapturing approach followed by high-throughput *** both of the ancestral red jungle fowl and the domestic chicken,the avian broiler,DNA methylation and gene expression profiles were described

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来源： cnki会议评论

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Earthquake Research in China 2010年第3期24卷 385-393页

作者： Fan Xiaoping li qinghe He Haibing Yang Congjie Jin Shumei Earthquake Administration of Jiangsu Province Nanjing 210014 China Earthquake Administration of Zhenjiang City Zhenjiang 212001 China

High-frequency S-wave seismogram envelopes of microearthquakes broaden with increasing travel distance,a phenomenon known as S-wave envelope broadening. Multiple forward scattering and diffraction for the random inhomogeneities along the seismic ray path are the main causes of S-wave envelope broadening,so the phenomenon of S-wave envelope broadening is used to study the inhomogeneity of the medium. The peak delay time of an S-wave,which is defined as the time lag from the direct S-wave onset to the maximum amplitude arrival of its envelope,is accepted to quantify S-wave envelope broadening. 204 small earthquake records in Changbaishan Tianchi volcano were analyzed by the S-wave envelope broadening algorithm. The results show that S-wave envelope broadening in the Changbaishan Tianchi volcano is obvious,and that the peak delay time of S-wave has a positive correlation with the hypocenter distance and frequency of the S-wave. The relationships between the S-wave peak delay time and the hypocenter distance for different frequency bands were obtained using the statistics method. The results are beneficial to the understanding of the S-wave envelope broadening phenomena and the quantitative research on the inhomogeneities of the crust medium in the Changbaishan Tianchi volcano region.

关键词： Changbaishan Tianchi volcano S-wave envelope broadening Peak delay timeof S-waves

The Crustal Velocity Structure Inference of Epicenter of the M8(1/2) Earthquake at Tancheng,Shandong,China,in 1668

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Earthquake Research in China 2014年第3期28卷 320-334页

作者： li qinghe Zhang Yuansheng Bao Haiying Jin Shumei Earthquake Administration of Jiangsu Province Lanzhou Institute of Seismology China Earthquake Administration

The deep structure features around Tancheng M8 /2 earthquake occurred in 1668 have been analyzed. Based on the crustal velocity structure obtained from travel-time tomographic inversion, especially the low velocity zones in middle crust and Moho depths, the deep velocity structure distribution in the area of 34° ~ 36°N, 118° ~ 119°E is scanned along the latitude, longitude and oblique directions, and the corresponding crustal velocity profiles are obtained. By comparison, we take the area with velocity features coincident to the deep structure of the 1668 Tancheng M8/2 earthquake as the deduced epicenter, which is at 34. 8° ~35. 2°N, 118. 2° ~ 118. 7°E, and the reasonable location is 35. isN, 118. 6°E, and the focal depth is 20km.

关键词： The 1668 Tancheng earthquake, Shandong Crustal velocity structure Velocity-depth profile tomography

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生态学:研究现代大学制度的新视角

辽宁教育研究 2007年第1期 23-25页

作者：李青合长江大学湖北荆州434023

高等教育需要多学科交叉进行研究,我国高等教育改革和现实发展需要建立现代大学制度,在生态整体观、系统观、动态平衡观、和谐价值观等生态观关照下建立现代大学制度将是一种新的研究视角。

关键词：生态学现代大学制度视角高等教育

Effects of Intraocular Lens Implantation without Viscoelastic Agents on Corneal Endothelial Cells

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International Journal of Clinical Medicine 2018年第1期9卷 1-7页

作者： Hao Wang Xueying Song Huichun Yang qinghe li Chang’e Hu Shaowen Qi Department of Ophthalmology PLA NO. 152 Hospital Pingdingshan China

Objective: To determine the protective effects of intraocular lens implantation without viscoelastic agents on corneal endothelial cells. Methods: Patients with age-related cataract were randomly divided into two groups: Group A (24 patients, 30 eyes) underwent phacoemulsification and intraocular lens implantation without viscoelastic agents, and group B (21 patients, 30 eyes) underwent phacoemulsification and intraocular lens implantation with viscoelastic agents. The corneal endothelial cell counts, percentages of hexagonal cells, and central corneal thicknesses were evaluated at 1 week, 1 month, and 6 months after surgery. Results: There was no significant difference in preoperative basic characteristics between the two groups (p > 0.05). The postoperative corneal endothelial cell count and percentage of hexagonal cells in both groups decreased compared with preoperative values at 1 week, 1 month, and 6 months after surgery, and the decrease of group A was significantly lower than that of group B at all time points (p p > 0.05). Conclusions: Compared with regular intraocular lens implantation, intraocular lens implantation without viscoelastic agents is less damaging to corneal endothelial cells, resulting in greater corneal safety.

关键词： Intraocular Lens Implantation Viscoelastic Agents Corneal Endothelial Cells