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Molecular Cloning and Expression of Polyphenoloxidase Genes from the Mushroom, Agaricus bisporus

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Agricultural Sciences in China 2011年第2期10卷 185-194页

作者： li nan-yi CAI Wei-ming JIN Qun-li QIN Qiao-ping RAN Fu-lai College of Agriculture and Food Science Zhejiang Agricultural and Forestry UniversityHangzhou 311300P.R.China Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhou 310021P.R.China Odessa College University of AveOdessa 79764USA

The polyphenoloxidase（PPO） is the key enzyme considered to be responsible for mushroom *** using homology cloning and rapid amplification of cDNA ends（RACE）,two new PPO genes and the corresponding cDNA were identified from the fruit bodies of Agaricus bisporus（AbPPO3 and AbPPO4,GenBank accession ***936494 and GU936493,respectively）.The genomic DNA sequences of AbPPO3 and AbPPO4 are 2 080 and 2 189 bp in length,respectively,encoding putative polypeptides of approximately 66 and 68 *** deduced amino acid sequences show characteristic features of two copper-binding domains conserved in the type III copper proteins including fungal polyphenol *** comparisons indicate that AbPPO3 and AbPPO4 present 55.3% similarity to each other（48% identity）.We also obtained more than 1.5-kb long sequences upstream of the start codon of the AbPPO3 and AbPPO4 and recognized them as their respective putative *** of the two PPO promoter regions show that they contain abundant cis-acting elements which are probably responsible for anaerobic induction,light,wound,stress,and auxin ***-quantitative RT-PCR results indicate that AbPPO3 and AbPPO4 were highly expressed in the mature fruit bodies and up-regulated after 2-d storage of *** results suggest that AbPPO3 and AbPPO4 may play roles in *** browning and pigmentation during development and postharvest storage and the elements in promoters may act as regulatory elements for the inducible expression of AbPPO3 and *** successful cloning and expression analysis of AbPPO3 and AbPPO4 warrant a further investigation on the structure and function of *** PPO which points to the possible targets for genetic manipulation.

关键词： polyphenoloxidase Agaricus bisporus tyrosinase promoter browning

Phylogenetic Analysis of Legionella Strains and Identification of Serogroups by lipopolysaccharide-and O-antigen-based PCR Assay

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Biomedical and Environmental Sciences 2021年第6期34卷 483-488页

作者： li yi nan REN Hong Yu ZHAO Na WANG Yan Qing li Dai QIN Tian State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102249China nanchang Center for Disease Control and Prevention Nanchang 330100JiangxiChina Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310021JiangsuChina

Legionella,a genus of pathogenic Gram-negative bacteria,is widely present in natural water sources and artificial water systems.A total of 65 species and>70 serogroups of Legionella have been characterized[1].lipopolysaccharide(LPS),the main component of the outer membrane of Legionella,is not only associated with toxicity,but also provides the basis for classification in serotyping[2].

关键词： Phylogenetic artificial outer

Isolation and Characterization of Vagococcus fluvialis from Bats

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Biomedical and Environmental Sciences 2021年第10期34卷 834-837页

作者： QIN Tian JIANG Lu Xi REN Hong Yu JIA Xue Yang NIE Xu Dong li yi nan State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Department of Respiratory Medicine Zhejiang Provincial People's HospitalPeople's Hospital of Hangzhou Medical CollegeHangzhou 310000ZhejiangChina

The genus Vagococcus was first described by *** initially consisted of a single species,*** species was isolated from chicken feces and river water and first described by Hashimoto et al.[1,2].Teixeira et *** *** from... 详细信息

关键词： initially fluvialis coccus

Microbial Community Structure of Casing Soil During Mushroom Growth

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Pedosphere 2009年第4期19卷 446-452页

作者： CAI Wei-Ming YAO Huai-ying FENG Wei-lin JIN Qun-li liU Yue-Yan li nan-yi ZHENG Zhong Department of Plant Protection Zhejiang University Hangzhou 310029 (China) Horticulture Institute Zhejiang Academy of Agricultural Sciences Hangzhou 310021 (China) Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition Zhejiang University Hangzhou 310029 (China)

The culturable bacterial population and phospholipid fatty acid （PLFA） profile of casing soil were investigated at different mushroom （Agaricus bisporus） cropping stages. The change in soil bacterial PLFAs was always accompanied by a change in the soil eulturable bacterial population in the first flush. Comparatively higher culturable bacterial population and bacterial PLFAs were found in the casing soil at the primordia formation stage of the first flush. There was a significant increase in the ratio of fungal to bacterial PLFAs during mushroom growth. Multivariate analysis of PLFA data demonstrated that the mushroom cropping stage could considerably affect the microbial community structure of the casing soil. The bacterial population increased significantly from casing soil application to the primordia formation stage of the first flush. Casing soil application resulted in an increase in the ratio of gram-negative bacterial PLFAs to gram-positive bacterial PLFAs, suggesting that some gram-negative bacteria might play an important role in mushroom sporophore initiation.

关键词： cropping stage culturable bacterial population phospholipid fatty acid

“科学地再现”:从“科学”的歧义性重审卢卡奇-布莱希特论争

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文艺理论与批评 2021年第3期 19-31页

作者：李轶男清华大学人文学院写作与沟通教学中心

以现实主义艺术的再现机制为框架,在"表象""实质"和其自身三个层面,卢卡奇与布莱希特展现出对"科学"的理解和运用差异。卢卡奇与布莱希特的论争,除去在具体议题上的对立,更重要的是在对"艺术如何作用于政治"这个问题上,着力点产生了视... 详细信息

以现实主义艺术的再现机制为框架,在"表象""实质"和其自身三个层面,卢卡奇与布莱希特展现出对"科学"的理解和运用差异。卢卡奇与布莱希特的论争,除去在具体议题上的对立,更重要的是在对"艺术如何作用于政治"这个问题上,着力点产生了视差。现实主义的认知功能将"科学"引入传统的艺术领域,挑战了艺术领域的诸多议题,也带入了政治斗争的急迫性与强度,从而激发出这一论争持久的"内在活力"。

关键词：科学再现卢卡奇布莱希特视差

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行业性行政垄断中的利益集团成本收益分析

中南财经政法大学学报 2008年第2期 34-39页

作者：李轶男山东大学经济学院山东济南250100

行业性行政垄断中的利益集团包括垄断企业利益集团、消费者利益集团、行政垄断实施者利益集团和非垄断企业利益集团。通过明确每个利益集团在行业性行政垄断下会发生哪些成本和收益,成本收益在不同利益集团之间如何转化,以及最终对社会... 详细信息

行业性行政垄断中的利益集团包括垄断企业利益集团、消费者利益集团、行政垄断实施者利益集团和非垄断企业利益集团。通过明确每个利益集团在行业性行政垄断下会发生哪些成本和收益,成本收益在不同利益集团之间如何转化,以及最终对社会总福利的影响结果。

关键词：行业性行政垄断利益集团成本收益

中国现代艺术思想的肇始:以李叔同、丰子恺、钱君匋为中心的考察

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艺术百家 2017年第5期33卷 150-156页

作者：李轶南东南大学艺术学院江苏南京210018

清末民初,时世动荡,干戈扰攘,仁人志士希冀找寻最有效的模式重塑国民性。李叔同、丰子恺、钱君匋师生三代围绕器识与文艺、运用跨学科交叉的方法昭示了鲜明的问题意识,对传统价值进行重估。他们以其独特卓越的艺术探索身姿,试图打破各... 详细信息

清末民初,时世动荡,干戈扰攘,仁人志士希冀找寻最有效的模式重塑国民性。李叔同、丰子恺、钱君匋师生三代围绕器识与文艺、运用跨学科交叉的方法昭示了鲜明的问题意识,对传统价值进行重估。他们以其独特卓越的艺术探索身姿,试图打破各门类艺术间的传统界限,探索实验新的表现途径与视觉艺术结构,在跨学科交叉视野中实现新高度的综合。他们的思考在中国现代艺术建立之初,起到了开创性、引领性和示范性作用,这种作用时至今日仍或显或隐地彰显。

关键词：艺术学理论中国艺术史现代艺术艺术思想肇始

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风流犹拍古人肩:拓印画里的传统中国

艺术百家 2019年第1期37卷 188-192页

作者：李轶南东南大学艺术学院江苏南京210018

艺术学家张道一先生是艺术理论界富有开创性、代表性的学者,他的新著《中国拓印画通览》于2014年被列入“十二五”国家重点出版物岀版规划项目,2016年又被列入国家出版基金资助项目。此书的问世弥补了中国拓印画整体研究的某些不足,对... 详细信息

艺术学家张道一先生是艺术理论界富有开创性、代表性的学者,他的新著《中国拓印画通览》于2014年被列入“十二五”国家重点出版物岀版规划项目,2016年又被列入国家出版基金资助项目。此书的问世弥补了中国拓印画整体研究的某些不足,对中国艺术史的研究具有示范性意义,对我国优秀传统艺术的继承与创新将产生积极、深远的影响。

关键词：传统中国古人风流基金资助项目 “十二五” 中国艺术史规划项目传统艺术

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中国古琴设计思想探析:以《琴史》为例

艺术百家 2012年第2期28卷 186-189页

作者：李轶南东南大学艺术学院江苏南京210018

《琴史》是我国现存最早的琴史专著[1],它不仅记录、总结了我国的琴史资料,而且蕴藏了丰富的古琴设计经验和设计思想。它的设计思想主要体现在三个方面:反映了阴阳五行观念、道德隐喻与礼仪特征;采用了取象比类的设计方法表述社会文化... 详细信息

《琴史》是我国现存最早的琴史专著[1],它不仅记录、总结了我国的琴史资料,而且蕴藏了丰富的古琴设计经验和设计思想。它的设计思想主要体现在三个方面:反映了阴阳五行观念、道德隐喻与礼仪特征;采用了取象比类的设计方法表述社会文化和观念形态;选材、装饰和形制皆法自然物象,善于提炼,精于取舍。反思中国古琴设计中映射的关于宇宙、社会和人生的有益思想,对今日的设计研究仍富有重要启发意义。

关键词：设计史艺术设计《琴史》设计思想文化观念