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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 500-506页

作者： Jun-Nian zheng~(1.3#*)Ya-Feng SUN~(2#)Dong-Sheng PEI~2 Jun-Jie {1.U~1.Jia-Cun CHEN~1.Wang {1.~1.Xiao-Qing SUN~1.Qi-Duo SHI~3 Rui-Fa HAN~3 Teng-Xiang MA~3 1.Laboratory of Urology,Affi{1.ated Hospital of Xuzhou Medical College,Xuzhou 221.02,china 2 Research Center for Biochemistry and Molecular Biology,Xuzhou Medical College,Xuzhou 221.02,china 3 Institute of Urology,tianjin Medical university,tianjin 300070,china LaboratoryofUrology AffiliatedHospitalofXuzhouMedicalCollegeXuzhou221002China ResearchCenterforBiochemist'ryandMolecularBiology XuzhouMedicalCollegeXuzhou221002China

Telomerase is an attractive molecular target for cancer therapy because it is present in mostmalignant cells but is undetectable in most {1. somatic *** telomerase consists of two subunits,an RNA component(hTR)and a human telomerase reverse transcriptase component(hTERT).Smallinterfering RNA(siRNA),one kind of RNA interferences,has been demonstrated to be an effective methodto inhibit target gene expression in human *** investigated the effects of siRNA targeting at both hTRand hTERT mRNA on the inhibition of telomerase activity in human renal carcinoma cells(HRCCs).Theproliferation and apoptosis of HRCCs were *** treatment of HRCCs using hTR and hTERTsiRNAs resulted in significant decrease of hTR mRNA,hTERT mRNA and hTERT *** siRNA canalso inhibit the telomerase activity and the proliferation of ***,they can induce apoptotic celldeath in a dose-dependent *** these findings,we propose that the inhibition of telomerase activityusing siRNA targeting hTR and hTERT might be a rational approach in renal cancer therapy.

关键词： hTERT hTR telomerase siRNA renal cell carcinoma proliferation apoptosis

Sequencing,annotation and comparative analysis of nine BACs of the giant panda(Ailuropoda melanoleuca)

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Science china({1.fe Sciences) 2010年第1期53卷 107-111页

作者： zheng Yang1,2,3,CAI Jing4,li JianWen3,6,li Bo3,liN RunMao3,tian Feng3,WANG Xiaoling3 & WANG Jun3,5 1Beijing Institute of Genomics,Chinese Academy of Sciences(CAS),Beijing 100029,china 2College of life Science,Graduate university of Chinese Academy of Sciences,Beijing 100049,china 3Beijing Genomics Institute at Shenzhen,Shenzhen 518083,china 4CAS-Max Planck Junior Research Group on Evolutionary Genomics,State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences(CAS),Kunming 650223,china 5department of Biology,university of Copenhagen,Copenhagen DK-2200,Denmark 6School of Biological Science and Engineering,South china university of Technology,Guangzhou 510006,china Beijing Institute of Genomics Chinese Academy of Sciences (CAS) Beijing China College of life Science Graduate University of Chinese Academy of Sciences Beijing China Beijing Genomics Institute at Shenzhen Shenzhen China CAS-Max Planck Junior Research Group on Evolutionary Genomics State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences (CAS) Kunming China School of Biological Science and Engineering South China University of Technology Guangzhou China department of Biology University of Copenhagen Copenhagen Denmark

A 1.-fold BAC library for the giant panda was constructed and nine BACs were selected to generate finish *** BACs could be used as a validation resource for the de novo assembly accuracy of the whole genome shotgun sequencing reads of the giant panda newly generated by Illumina GA sequencing *** Sanger sequencing,assembly,annotation and comparative analysis were carried out on the selected BACs of a joint length 878 *** search and de novo prediction methods were used to annotate genes and *** protein coding genes were predicted,seven of which could be functionally *** seven genes have an average gene size of about 41.kb,an average coding size of about 1.2 kb and an average exon number of 6 per ***,seven tRNA genes were *** 27 percent of the BAC sequence is composed of repeats.A phylogenetic tree was constructed using a neighbor-join algorithm across five species,including the giant panda,human,dog,cat and mouse,which reconfirms dog as the most closely related species to the giant *** results provide detailed sequence and structure information for new genes and repeats of the giant panda,which will be helpful for further studies about the giant panda.

关键词： giant panda BAC sequencing assembly annotation evolution

DEAD-box helicase 1.(DDX1.)protects cardiac function by promoting mitochondrial homeostasis in heart failure

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Signal Transduction and Targeted Therapy 2024年第6期9卷 2774-2790页

作者： mingjing Yan Junpeng Gao ming Lan Que Wang Yuan Cao Yuxuan zheng Yao Yang Wenlin li Xiaoxue Yu Xiuqing Huang lin Dou Bing liu Junmeng liu Hongqiang Cheng Kunfu Ouyang Kun Xu Shenghui Sun Jin liu Weiqing Tang Xiyue Zhang Yong Man liang Sun Jianping Cai Qing He Fuchou Tang Jian li Tao Shen The Key Laboratory of Geriatrics Beijing Institute of GeriatricsInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing Hospital/National Center of Gerontology of National Health CommissionBeijing 100730China Peking university Fifth School of C{1.nical Medicine Beijing 100730China department of Laboratory Medicine Huashan HospitalShanghai Medical CollegeFudan UniversityShanghai 200040China Biomedical Pioneering Innovation Center School of Life SciencesPeking UniversityBeijing 100871China Emergency Center Zhongnan Hospital of Wuhan UniversityWuhan 430071China department of Cardiology Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Graduate School of Peking Union Medical College Beijing 100730China department of Health Care Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Peking-Tsinghua Center for life Sciences Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China department of Pathology and Pathophysiology and department of Cardiology at Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou 310058China department of Cardiovascular Surgery Peking University Shenzhen HospitalShenzhen 518036China Experimental Technology Center for life Sciences at Beijing {1. university Beijing 100875China

DEAD-box helicase 1.(DDX1.)is a typical member of the DEAD-box family with transcriptional cofactor *** DDX1. is abundantly expressed in the myocardium,its role in heart is not fully *** generated cardiomyocyte-specific Ddx1.-knockout mice(Ddx1.-cKO),cardiomyocyte-specific Ddx1. transgenic mice(Ddx1.-Tg),and various models of cardiomyocyte injury and heart failure(HF).DDX1. is downregulated in the myocardium of mouse models of heart failure and cardiomyocyte ***-specific knockout of Ddx1. promotes autophagic flux blockage and cardiomyocyte apoptosis,leading to progressive cardiac dysfunction,maladaptive remodeling and progression to heart *** of DDX1. expression in cardiomyocytes protects cardiac function under pathological *** studies showed that DDX1. can bind to the transcriptional repressor B-cell lymphoma 6(BCL6)and inhibit the expression of dynamin-related protein 1.DRP1..When DDX1. expression is reduced,transcriptional repression of BCL6 is attenuated,leading to increased DRP1.expression and mitochondrial fission,which in turn leads to impaired mitochondrial homeostasis and heart *** also investigated the correlation of DDX1. expression with cardiac function and DRP1.expression in myocardial biopsy samples from patients with heart *** findings suggest that DDX1. protects cardiac function by promoting mitochondrial homeostasis through the BCL6-DRP1.pathway in heart failure.

关键词： DDX1. homeostasis cardiac

A Practical Reconstruction Algorithm in 2D Industrial CT

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中国体视学与图像分析 2005年第3期 189-193页

作者： li Hui 1.2, {1. Jie 2, ZHANG Zhao{1. 3 (1. department of Education Technology, Capital normal university, Beijing, 1.0037, china 2. Medical Image Processing Group, Key Laboratory of Complex Systems and Intelligence Science, Institute of Automation, Chinese Academy of Sciences, P.O.Box 2728, Beijing, 1.0080, china 3. department of Information Science, National Natural Science Foundation of china, Beijing, 1.0085, china)

Computed tomography plays an important role in industrial non-destructive testing, medical applications, astronomy and many other fields to look inside the scanned object and to analysis its inner structures. A non-destructive testing software have been developed to efficiently detect inner flaws of space industrial components. As the core of our software, reconstruction algorithms including preprocess of raw data, re-arrange algorithm and filtered back-projection algorithms have been described in detail in this article. With real raw data from CASC of{1., experimental results verified the applied reconstruction algorithm in our software. Furthermore, forward algorithms simulating generation of fan-beam raw data are also presented in this article.

关键词： Reconstruction algorithms industrial CT imaging data preprocess

Friend or foe? Using eye-tracking technology to investigate the visual discrimination ability of giant pandas

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Current Zoology 2024年第4期70卷 430-439页

作者： Xinrui Huang Guo li Guiquan Zhang Zixiang li lin Zhao Mengdie Zhu Qinghua Xiang Xuefeng liu Mei tian Hemin Zhang Christina D.Buesching Dingzhen liu Ministry of Education Key Laboratory of Biodiversity Science and Ecological EngineeringDepartment of EcologyCollege of Life SciencesBeijing Normal UniversityBeijing 100875China Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park China Conservation and Research Center for the Giant PandaSichuan 623004China School of Computer and Information Technology Beijing Jiaotong UniversityBeijing 100044China Beijing Key Laboratoryof CaptiveWildlife Technology Beijing ZooBeijing100044China department of Biology Irving K.Barber Faculty of ScienceThe University of British ColumbiaOkanagan CampusKelownaBCCanada

The role that visual discriminative ability plays among giant pandas in social communication and individual discrimination has received less attention than olfactory and auditory ***,we used an eye-tracker technology to investigate pupil fixation patterns for 8 captive male giant pandas Ailuropoda *** paired images(N=26)of conspecifics against:1.sympatric predators(gray wolves and tigers),and non-threatening sympatric species(golden pheasant,golden snub-nosed monkey,takin,and red panda),2)conspecifics with atypical fur colora-tion(albino and brown),and 3)zookeepers/non-zookeepers wearing either work uniform or plain *** each session,we tracked the pan-da's pupil movements and measured pupil first fixation point(FFP),fixation latency,total fixation count(TFC),and duration(TFD)of attention to each ***,pandas exhibited similar attention(FFPs and TFCs)to images of predators and non-threatening sympatric *** of golden pheasant,snub-nosed monkey,and tiger received less attention(TFD)than images of conspecifics,whereas images of takin and red panda received more attention,suggesting a greater alertness to habitat or food competitors than to potential ***'TFCs were greater for images of black-white conspecifics than for albino or brown phenotypes,implying that familiar color elicited more *** reacted differently to images of men versus *** images of women only,pandas gave more attention(TFC)to familiar combinations(uniformed zookeepers and plain-clothed non-zookeepers),consistent with the familiarity *** pandas can use visual perception to discriminate intra-specifically and inter-specifically,including details of human appearance,has applications for panda conservation and captive husbandry.

关键词： eye movement tracker giant panda images total fixation duration total pupil fixation count visual discrimination.

正弦波电磁场激活一氧化氮信号通路促进大鼠成骨细胞成熟与矿化

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生物化学与生物物理进展 2011年第4期38卷 331-337页

作者：程国政葛宝丰陈克明李志锋周建韦哲兰州军区兰州总医院骨科研究所兰州730050 兰州军区兰州总医院医学工程科兰州730050

研究正弦波电磁场(SEMF)促进成骨细胞成熟矿化是否与一氧化氮(NO)信号通路相关.首先检测成骨细胞经正弦电磁场作用0h、0.5h、1.、1.5h、2h、2.5h、3h、3.5h和4h后一氧化氮合酶(NOS)的活性,以探明电磁场是否影响NO的合成;其次,在细胞培... 详细信息

研究正弦波电磁场(SEMF)促进成骨细胞成熟矿化是否与一氧化氮(NO)信号通路相关.首先检测成骨细胞经正弦电磁场作用0h、0.5h、1.、1.5h、2h、2.5h、3h、3.5h和4h后一氧化氮合酶(NOS)的活性,以探明电磁场是否影响NO的合成;其次,在细胞培养液中加入NOS的阻断剂L-NAME以阻断NO信号通路,观察电磁场促进骨形成作用是否受到影响.结果发现,经正弦波电磁场处理后,NOS活性升高,在0.5h达到峰值,与空白对照组差异极显著(P1.,Osterix基因的表达量、碱性磷酸酶活性和钙化结节数等均显著高于对照组.L-NAME组各项指标均低于空白对照组,SEMF+L-NAME组则略高于空白对照组而低于SEMF组.以上结果表明SEMF促进成骨细胞成熟矿化过程中NO信号通路被激活,如该通路被抑制,则SEMF的促成骨作用被抵消.

关键词：正弦波电磁场成骨细胞分化成熟

Modular lie Superalgebras of H-type

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Acta Mathematicae Applicatae Sinica 2004年第4期 683-694页

作者： Yong zheng ZHANG {1. of Mathematics,Harbin normal university,Harbin 1.0080,P.R.china

In this article the Z-graded transitive modular lie superalgebra -1.li,whose repre-sentation of lin lis isomorphic to the natural representation of osp(L),is determined.

关键词： Modular lie superalgebra Divided power algebra Exterior algebra Flag

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光子晶体光纤的导波模式与色散特性

物理学报 2003年第11期52卷 2811-2817页

作者：李曙光刘晓东侯蓝田燕山大学红外光纤与传感研究所秦皇岛066004 大连民族学院机电信息工程系大连116600

利用有效折射率方法基于标量近似理论对光子晶体光纤的传播模式和色散特性进行了数值模拟 ,发现通过调节光纤包层的空气填充率或包层空气穴节距及其有效芯径可以在很宽的波长范围实现单模传播 ,可以设计零色散波长小于 1.2 7μm的光子... 详细信息

利用有效折射率方法基于标量近似理论对光子晶体光纤的传播模式和色散特性进行了数值模拟 ,发现通过调节光纤包层的空气填充率或包层空气穴节距及其有效芯径可以在很宽的波长范围实现单模传播 ,可以设计零色散波长小于 1.2 7μm的光子晶体光纤和在较宽的波段接近于零色散的色散平坦光纤 ,以及具有较大的正常色散值的色散补偿光纤 .

关键词：光子晶体光纤导波模式色散特性有效折射率标量近似色散补偿光纤光子带隙效应数值模拟

Association between QRS voltages and amyloid burden in patients with cardiac amyloidosis

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Chinese Medical Journal 2024年第3期137卷 365-367页

作者： Jing-Hui li Changcheng li Yucong zheng Kai Yang Yan Huang Huixin Zhang Xianmei li Xiuyu Chen linlin Dai tian Lan Yang Sun Minjie Lu Shihua Zhao department of Magnetic Resonance Imaging Fuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China department of Radiology Taian City Central Hospital(The Affiliated Taian City Central Hospital of Qingdao University)Tai’anShandong 271099China department of Radiology Tsinghua University HospitalTsinghua UniversityBeijing 100084China department of Radiology The Fifth People’s Hospital of Huai’anHuai’anJiangsu 223001China department of Radiology Geriatric Hospital of Nanjing Medical UniversityNanjingJiangsu 210000China department of Cardiology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China department of Pathology Fuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

To the Editor:Cardiac amyloidosis(CA)is an infiltrative cardiomyopathy characterized by extracellular deposition of misfolded insoluble amyloid fibrils.^([1.)Electrocardiography(ECG)is an essential diagnostic test characterized by low QRS voltages in CA,observed in 20–74%of patients.^([2])Cipriani et al^([3])reported that low QRS voltages could independently predict cardiovascular *** the mechanisms underlying low QRS voltages in CA remain unclear.

关键词： amyloid patients death.

Emergency rescue of a patient with hemorrhagic shock caused by superior mesenteric artery rupture:A case report

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World Journal of Clinical Cases 2024年第18期12卷 3567-3574页

作者： Xian-Ping lin Xin-li Guo He-Feng tian zheng-Rong Wu Wei-Jie Yang Hong-Ying Pan Nursing department Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310020Zhejiang ProvinceChina department of General Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310020Zhejiang ProvinceChina department of Obstetrics and Gynecology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310020Zhejiang ProvinceChina

BACKGROUND Superior mesenteric artery(SMA)injuries rarely occur during blunt abdominal injuries,with an incidence of35 beats/min),and blood pressure as low as 20/1. mmHg(1.mmHg=0.1.3 kPa).Computed tomography revealed ... 详细信息

BACKGROUND Superior mesenteric artery(SMA)injuries rarely occur during blunt abdominal injuries,with an incidence of1..The clinical manifestations mainly include abdominal hemorrhage and peritoneal irritation,which progress rapidly and are easily *** and accurate diagnosis and timely effective treatment are greatly significant in managing emergent *** report describes emergency rescue by a multidisciplinary team of a patient with hemorrhagic shock caused by SMA *** SUMMARY A 55-year-old man with hemorrhagic shock presented with SMA *** admission,he showed extremely unstable vital signs and was unconscious with a laceration on his head,heart rate of 1.3 beats/min,shallow and fast breathing(frequency>35 beats/min),and blood pressure as low as 20/1. mmHg(1.mmHg=0.1.3 kPa).Computed tomography revealed abdominal and pelvic hematocele effusion,suggesting active *** patient was suspected of partial rupture of the distal SMA *** patient underwent emergency mesenteric artery ligation,scalp suture,and liver laceration *** view of conditions with acute onset,rapid progression,and high bleeding volume,key points of nursing were conducted,including activating emergency protocol,opening of the green channel,and arranging relevant examinations with various medical staff for quick *** seamless collaboration of the multidisciplinary team helped shorten the preoperative preparation *** laparotomy exploration and mesenteric artery ligation were performed to mitigate hemorrhagic shock while establishing efficient venous accesses and closely monitoring the patient’s condition to ensure hemodynamic *** measures were taken to avoid intraoperative hypothermia and *** After 3.5 h of emergency rescue and medical care,bleeding was successfully controlled,and the patient’s condition was ***,the patient was transferred to the intensive care unit for continuous mon

关键词： Superior mesenteric artery Hemorrhagic shock Arterial ligation Emergency nursing Case report