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Impact of Seed Halopriming on Germination,Morphological Traits,and Cry1Ac Gene Expression in Bt Cotton(Gossypium hirsutum)

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Phyton-International Journal of Experimental Botany 2025年第1期94卷 229-241页

作者： Wenqi Shi Binyameen Bin Shafqat Muhammad Arif Ayesha Fazal Nawaz Muhammad Amir Zia Xu ling Dingli Hong Ruhong Xu luhua li College of Agriculture Guizhou UniversityGuiyang550025China Faculty of Medicine and Health Technology Tampere UniversityTampere33720Finland Department of life Sciences University of TriesteVia Licio Giorgieri 5Trieste34127Italy National Institute for Genomics and Advanced Biotechnology(NIGAB) National Agricultural Research Centre(NARC)Islamabad45500Pakistan

Seed priming is an effective seed pretreatment technology that enhances germination and overall crop performance by optimizing seed hydration and metabolic processes before *** quality is a critical determinant of cotton(Gossypium hirsutum)crop performance,influencing germination,plant vigor,and *** study evaluates the effects of seed priming with potassium salts(1%and 2%KCl and K2SO4)on germination,morphological traits,and Cry1Ac gene expression in three Bt cotton cultivars(IUB-2013,NIAB-878B,FH-142)as Cry1Ac enhance the pest resistance in Bt cotton and reduce the plant’s dependence on chemical *** were primed for six hours,air-dried,and sown in the *** rates,plant height,number of bolls per plant,boll weight,seed cotton yield,and ginning outturn(GOT)were assessed at crop ***1Ac gene expression was quantified to explore the influence of priming treatments on transgene *** demonstrated that 1%K2SO4 priming significantly enhanced germination and yield-related traits,with Cry1Ac expression peaking in the IUB-2013 cultivar under 1%K2SO4 *** findings suggest that potassium-based halopriming improves cotton seedling establishment and Bt gene *** study addresses the critical gaps in understanding the effects of seed halopriming on morphological traits,germination,and expression of the Cry1Ac gene in Bt cotton while providing a novel eco-friendly and cost-effective halopriming approach,offering the potential to improve cotton production.

关键词： Cotton seed priming halopriming Cry1Ac Bt cotton germination yield traits

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Boron nitride nanotube reinforced polyurethane composites

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Progress in Natural Science:Materials International 2013年第2期23卷 170-173页

作者： luhua li Ying Chen Zbigniew H. Stachurski Institute for Frontier Materials Deakin University Geelong Waurn Ponds Campus Research School of Engineering College of Engineering and Computer Science The Australian National University

Bulk-sized boron nitride nanotube (BNNT) reinforced polyurethane (PU) composites at different volume contents have been produced. A large quantity of BNNT fillers are synthesized by a boron ink method. Compared to the neat PU, the 0.5 vol% and 2.0 vol% BNNT reinforced composites show 38.2% and 6.3% increases in compressive modulus, respectively. The relatively less enhanced compressive modulus of the 2.0 vol% composite may be due to the agglomerations of nanotubes at high volume percentages. Contrary to normal behaviour, the composites show decreasing Rockwell (HRR) hardness values with an increasing volume fraction.

关键词： Composite Boron nitride nanotube Compressive modulus Rockwell hardness

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小麦TaPBF3-5D的基因克隆及生物信息学分析

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麦类作物学报 2025年第2期45卷 141-148页

作者：邓云颢李鲁华徐灵王忠妮洪鼎立邰成江徐如宏贵州大学农学院贵州贵阳550025 国家小麦改良中心贵州分中心贵州贵阳550025 贵州省农业科学院水稻研究所贵州贵阳550025

PBF因子是小麦胚乳特异基因表达调控蛋白因子之一,在调控小麦种子贮藏蛋白基因表达方面有着重要作用。本研究以小麦种质ZY96-3为材料,利用RT-PCR技术克隆获得TaPBF3-5D的CDS序列,对其进行了生物信息学和基因表达分析。结果表明,TaPBF3-5... 详细信息

PBF因子是小麦胚乳特异基因表达调控蛋白因子之一,在调控小麦种子贮藏蛋白基因表达方面有着重要作用。本研究以小麦种质ZY96-3为材料,利用RT-PCR技术克隆获得TaPBF3-5D的CDS序列,对其进行了生物信息学和基因表达分析。结果表明,TaPBF3-5D基因CDS序列共有909 bp,编码302个氨基酸;其编码的蛋白为一个非跨膜的不稳定性亲水蛋白,无信号肽,共含36个磷酸位点;预测其定位于细胞核;通过系统进化树以及多重序列比对,TaPBF3-5D与来自于普通小麦中国春的PBF在亲缘性上更为接近。经荧光定量分析,TaPBF3-5D基因在根、茎、叶和籽粒中均有表达,以籽粒中表达最高,其次是叶和茎,根中表达最低。这进一步说明TaPBF3-5D基因参与调控小麦籽粒贮藏蛋白基因表达,调节籽粒发育。

关键词： ZY96-3 TaPBF3-5D 生物信息学基因克隆

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Surface wetting processing on BNNT films by selective plasma modes

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Chinese Science Bulletin 2013年第27期58卷 3403-3408页

作者： li ling liU XiaoWei DAI XiuJuan J li luhua CHEN Ying MEMS Center Harbin Institute of Technology Key Laboratory of Micro-Systems and Micro-Structures Manufacturing Ministry of Education Institute for Frontier Materials Deakin University

The wettability of boron nitride nanotube (BNNT) films was modified using a combination of pulsed and continuous wave (CW) mode plasma. The combined mode effectively modified the wettability of BNNT films and kept the nanostructures intact. The BNNT films changed from superhydrophobic to superhydrophilic after combined mode treatment at 600 W min. In contrast, the contact angle controllable decreased linearly in a controllable way with increasing energy input before eventually becoming superhydrophilic after 1000 W min of CW mode treatment. A high concentration of graft functional groups formed, along with point defects. More point defects formed when using combined modes and higher energy input. Mainly amine functional groups were grafted by combined mode plasma, while the CW mode plasma led to more formation of amide and imine on the BNNTs. Research into controllable wettability and selection of grafted functional groups should enable promising applications of BNNTs in composites and biology in the future.

关键词：硼氮纳米管薄膜表面等离子体接枝湿处理血浆氮化硼纳米管模式处理超亲水性

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Ultrasonic-assisted Mesoporous Silica Nanoparticle-mediated Exogenous Gene Stable Expression in Tobacco

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Chemical Research in Chinese Universities 2017年第6期33卷 912-916页

作者： WANG Zhongni liU Huijing li luhua li Quanliang WANG Xiuran JIANG Yuan FU Yuqin LU Changli Engineering Research Center of Bioreactor and Pharmaceutical Development Ministry of EducationCollege of Life Science Jilin Agricultural University Changchun 130018 P R. China Faculty of Chemistry Northeast Normal University Changchun 130024 P. R. China Guizhou Sub-center of National Wheat Improvement Center Guizhou University Guiyang 550025 P. R. China

Although nanotechnology is considered to be one of the most important technologies to promote social and economic development in the twenty-first century, its application in agriculture is relatively few compared with those in other fields. In this article, plasmid carrying GUS gene was successfully delivered into tobacco（Nicotiana tabacum） by mesoporous silica nanoparticles（MSNs） modified with positively charged poly-L-lysine（PLL） with the assist of ultrasonic method. Stable transformation was confirmed by polymerase chain reaction（PCR） detection and GUS histochemical staining. Meanwhile, we also studied the factors that could enhance the genetic transformation efficiency. The result suggests that the callus receptor and a suitable DNA/MSNs ratio contribute a lot to the transformation efficiency. In a word, our research provides an efficient and cost-effective method for gene delivery to plant.

关键词： Mesoporous silica nanoparticle Gene delivery Tobacco

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Silica Nanoparticles-mediated Stable Genetic Transformation in Nicotiana tabacum

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Chemical Research in Chinese Universities 2015年第6期31卷 976-981页

作者： FU Yuqin li luhua WANG Hui JIANG Yuan liU Huijing CUI Xiyan WANG Piwu LU Changli College of life Science Jilin Agricultural University Changchun 130018 P. R. China Faculty of Chemistry Northeast Normal University Changchun 130024 P. R. China

Nanoparticles as gene carriers become popular in the mammalian cells, whereas the application of them in plant cells is still very limited. Herein lies a report on silica nanoparticles（SiNPs） modified with positively charged poly-L-lysine（PLL） successfully delivering plasmid-encoded β-glucuronidase（GUS） gene into tobacco with the help of gene gun. The stable transgenic tobacco plants mediated by SiNPs can be obtained. Furthermore, we revealed the quantity of gene and types of receptor materials could affect the expression efficiency. In comparison to conven- tional gold particles-mediated transformation, the silica nanoparticles-mediated stable genetic transformation enhances transformation efficiency, potentially overcoming transgenic silencing. Our results demonstrate the great potential of SiNPs as gene carrier in plant genetic transformation and prove a novel approach for plant genetic decoration.

关键词： Silica nanoparticle Gene carrier Transformation Stable expression

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Radial vibration of a slit-type annular ultrasonic concentrator

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Chinese Journal of Acoustics 2015年第2期34卷 153-165页

作者： liU Shiqing GUI Fengli WANG Jiatao YANG Xianli li luhua Applied Acoustics Institute Zhejiang Normal University

The radial vibration characteristic of an annular ultrasonic concentrator with multi- slits is studied. Based on the electro-mechanical analogy and by introducing an area ratio coef- ficient, the radial vibrational equivalent circuit and the frequency equation of the concentrator are derived. The radial displacement amplitude magnification factor of it is obtained. By nu- merical calculating, the relationship between the radial displacement amplitude magnification factor, the resonance frequency of the concentrator at the first and second resonance and its radius ratio are investigated. Also, the relationship between the radial displacement amplitude magnification factor, the resonance frequency of the concentrator and the length, the angle and the number of the silts are analyzed. It is illustrated that a maximum of displacement ampli- tude magnification factor of the concentrator appears with the increase of the radius ratio, and the amplitude magnification factor increase as the length, the angle and the number of the silts increase, while the radial resonance frequency of the concentrator decreases as the length, the angle and the number of the silts increase. The theoretical resonance frequencies are in good agreement with that of the FEM simulations and measurement.

关键词： type Radial vibration of a slit-type annular ultrasonic concentrator

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镶拼式复合压电超声换能器的径向振动特性

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机械工程学报 2016年第16期52卷 22-26页

作者：李陆化刘世清李丹浙江师范大学应用声学研究所金华321004

对一种镶拼式复合压电超声换能器的径向振动特性进行分析。该镶拼式复合压电超声换能器由弹性内芯、矩形压电陶瓷晶堆环组及金属预应力管径向复合而成。采用压电陶瓷晶堆,可大幅提高换能器的功率容量及有效机电耦合系数。依据机电类比原... 详细信息

对一种镶拼式复合压电超声换能器的径向振动特性进行分析。该镶拼式复合压电超声换能器由弹性内芯、矩形压电陶瓷晶堆环组及金属预应力管径向复合而成。采用压电陶瓷晶堆,可大幅提高换能器的功率容量及有效机电耦合系数。依据机电类比原理,得出换能器的机电等效电路和共振频率方程;探讨换能器的第1、2阶共振和反共振频率及机电耦合系数与其几何尺寸之间的关系,并进行有限元仿真分析和试验测试。研究表明,换能器第1阶径向共振及反共振频率随其内芯内径及预应力管外径增大而降低;第2阶共振频率随内芯内径增大存在极小值,但随预应力管外径增大而下降,并且第2阶共振模式的有效机电耦合系数较高。对换能器共振频率的测试和仿真表明,理论与试验及仿真结果相符合。

关键词：径向振动功率超声镶拼正多边形压电换能器

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