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Analysis of the Genetic relationships in Chinese Ziziphus with SrAp Markers

agricultural Sciences in china 2010年第9期9卷 1278-1284页

作者： li li, peng jian-ying and bai rui-xia college of horticulture, agricultural university of hebei, baoding 071001, p.r.china college of horticulture Agricultural University of Hebei Baoding 071001 P.R. China

The phylogenic and genetic relationships in germplasm resources of Chinese Ziziphus were studied at molecular levels, for providing new molecular evidences of classification, protection, and utilization of germplasm resources of Ziziphus. The sequence-related amplified polymorphism （SrAp） was assessed to analyse the genetic relationships among 14 species of Ziziphus, 11 cultivars of Z. jujuba Mill., and one outgroup. A total of 580 DNA bands were amplified by 19 selective primers, 570 of which （98.28%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 30. The genetic similarities of 26 sample materials were between 0.22 and 0.99. UpGMA method cluster analysis showed that 26 sample materials were classified into six cluster groups with the genetic similarity of 0.28. The results showed that SrAp technique is efficient in studying genetic relationships among Ziziphus, Z. jujuba Mill. and Z. acidojujuba C. Y. Cheng et M. J. liu which should be treated as one species, and further infraspecific classification of Z. jujuba Mill. should be classified into two subspecies; the scientific names of new taxa, Chinese jujube, and wild jujube were Ziziphus jujuba Mill. subsp, jujuba and Ziziphus jujuba Mill. subsp, spinosa （Bunge） J. Y. peng, X. Y. li et L. li, respectively. Z. xiangchengensis Y. *** et p. K. Chou, Z. montana W.W. Smith and Z. mairei Dode might be originated from same ancestry. Z xiangchengensis Y. L. Chen et p. K. Chou and Z. montana W.W. Smith should be treated as one species. The infrasubspecific taxon of Z. jujuba Mill. was not suitable to set up varieties.

关键词： Ziziphus Mill. genetic relationships SrAp cluster analysis

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Crystal facet-dependent CO_(2) cycloaddition to epoxides over ZnO catalysts

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Frontiers of Chemical Science and Engineering 2024年第5期18卷 49-59页

作者： Yongjian Wei ying li Yunfei Xu yinghui Sun Tong Xu Haiou liang Jie bai Inner Mongolia Key Laboratory of Industrial Catalysis Hohhot 010051China college of Chemical Engineering Inner Mongolia University of TechnologyHohhot 010051China

With regard to green chemistry and sustainable development,the fixation of CO_(2) into epoxides to form cyclic carbonates is an attractive and promising pathway for CO_(2) *** oxides,renowned as promising eco-friendly catalysts for industrial production,are often undervalued in terms of their impact on the CO_(2)addition *** this work,we successfully developed ZnO nanoplates with(002)surfaces and ZnO nanorods with(100)surfaces via morphology-oriented regulation to explore the effect of crystal faces on CO_(2) *** quantitative data obtained from electron paramagnetic resonance spectroscopy indicated that the concentration of oxygen vacancies on the ZnO nanoplate surfaces was more than twice that on the ZnO nanorod *** functional theory calculations suggested that the(002)surfaces have lower adsorption energies for CO_(2) and epichlorohydrin than the(100)*** a result,the yield of cyclochloropropene carbonate on the ZnO nanoplates(64.7%)was much greater than that on the ZnO nanorods(42.3%).Further evaluation of the reused catalysts revealed that the decrease in the oxygen vacancy concentration was the primary factor contributing to the decrease in catalytic *** on these findings,a possible catalytic mechanism for CO_(2) cycloaddition with epichlorohydrin was *** work provides a new idea for the controllable preparation of high-performance ZnO catalysts for the synthesis of cyclic carbonates from CO_(2) and epoxides.

关键词： carbon dioxide cycloaddition zinc oxide crystal face oxygen vacancy

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predictive performance of the variation rate of the driving pressure on the outcome of invasive mechanical ventilation in patients with acute respiratory distress syndrome

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Chinese Journal of Traumatology 2024年第2期27卷 107-113页

作者： Hui-Dan Jing Jun-ying Tian Wei li Bing-ling He Hong-Chao li Fu-xia jian Cui Shang Feng Shen Department of Intensive Care Unit The Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004China Department of Intensive Care Unit Army Medical Center of PLAChongqing400042China college of Foreign Languges Chongqing Medical UniversityChongqing400016China

purpose:To assess the value of the driving pressure variation rate(Δp%)in predicting the outcome of weaning from invasive mechanical ventilation in patients with acute respiratory distress ***:In this case-control study,a total of 35 patients with moderate-severe acute respiratory distress syndrome were admitted to the intensive care unit between January 2022 and December 2022 and received invasive mechanical ventilation for at least 48 h were *** were divided into successful weaning group and failed weaning group depending on whether they could be removed from ventilator support within 14 *** measures including driving pressure,paO2:FiO2,and positive end-expiratory pressure,*** assessed every 24 h from day 0 to day 14 until successful weaning was *** measurement data of non-normal distribution were presented as median(Q1,Q3),and the differences between groups were compared by Wilcoxon rank sum *** categorical data use the Chi-square test or Fisher's exact test to *** predictive value ofΔp%in predicting the outcome of weaning from the ventilator was analyzed using receiver operating characteristic ***:Of the total 35 patients included in the study,17 were successful vs.18 failed in weaning from a ventilator after 14 days of mechanical *** cut-off values of the medianΔp%measured by Operator 1 *** 2 in the first 4 days were≥4.17%and 4.55%,respectively(prea under curve of 0.804(sensitivity of 88.2%,specificity of 64.7%)and 0.770(sensitivity of 88.2%,specificity of 64.7%),*** was a significant difference in mechanical ventilation duration between the successful weaning group and the failure weaning group(8(6,13)vs.12(7.5,17.3),p=0.043).The incidence of ventilator-associated pneumonia in the successful weaning group was significantly lower than in the failed weaning group(0.2‰vs.2.3‰,p=0.001).There was a significant difference noted between these

关键词： Driving pressure Driving pressure variation rate Acute respiratory distress syndrome Mechanical ventilation

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Epidemiological Surveillance: Genetic Diversity of rotavirus Group A in the pearl river Delta, Guangdong, china in 2019

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Biomedical and Environmental Sciences 2024年第3期37卷 278-293页

作者： jianG Jie ying liANG Dan WANG li xiaO Yun liANG Yu Feng KE Bi xia SU Juan xiaO Hong WANG Tao ZOU Min li Hong jian KE Chang Wen School of public Health Shantou UniversityShantou 515063GuangdongChina Guangdong provincial Center for Disease Control and prevention Guangdong Workstation for Emerging Infectious Disease Control and PreventionGuangzhou 511430GuangdongChina Guangdong provincial Key Laboratory of Virology Institute of Medical MicrobiologyJinan UniversityGuangzhou 510632GuangdongChina School of public Health Sun Yat-sen UniversityGuangzhou 510275GuangdongChina School of public Health Southern Medical UniversityGuangzhou 510515GuangdongChina Shantou university Medical college Shantou 515041GuangdongChina

Objective This study aimed to understand the epidemic status and phylogenetic relationships of rotavirus group A(rVA)in the pearl river Delta region of Guangdong province,*** This study included individuals aged 28 days–85 years.A total of 706 stool samples from patients with acute gastroenteritis collected between January 2019 and January 2020 were analyzed for 17 causative pathogens,including rVA,using a Gastrointestinal pathogen panel,followed by genotyping,virus isolation,and complete sequencing to assess the genetic diversity of *** The overall rVA infection rate was 14.59%(103/706),with an irregular epidemiological *** proportion of co-infection with rVA and other pathogens was 39.81%(41/103).Acute gastroenteritis is highly prevalent in young children aged 0–1 year,and rVA is the key pathogen circulating in patients 6–10 months of age with diarrhea.G9p[8](58.25%,60/103)was found to be the predominant genotype in the rVA strains,and the 41 rVA-positive strains that were successfully sequenced belonged to three different rVA genotypes in the phylogenetic *** analysis showed that gene reassortment events,selection pressure,codon usage bias,gene polymorphism,and post-translational modifications(pTMs)occurred in the G9p[8]and G3p[8]*** This study provides molecular evidence of rVA prevalence in the pearl river Delta region of china,further enriching the existing information on its genetics and evolutionary characteristics and suggesting the emergence of genetic *** the surveillance of genotypic changes and gene reassortment in rVA strains is essential for further research and a better understanding of strain variations for further vaccine development.

关键词： Infectious diarrheal disease rotavirus phylogenetic analysis Gene rearrangement Codon usage bias Genetic diversity

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One-pot and One-step Cu(0)-mediated reversible-Deactivation radical polymerization of N-Isopropylacrylamide(NIpAM) in Water

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Chinese Journal of polymer Science 2024年第1期42卷 1-6,I0004页

作者： Zi-Shan li Jing Lyu Bei Qiu ying-Hao li ruth Foley ri-jian Song Melissa Johnson Qiang Geng Wen-Xin Wang Institute of precision Medicine(AUST-IpM) Anhui University of Science and TechnologyHuainan 232001China Charles Institute of Dermatology School of MedicineUniversity College DublinDublin D04 V1W8Ireland

Copper(0)-mediated reversible-deactivation radical polymerization(Cu(0)-mediated rDrp) of the water-soluble monomer Nisopropylacrylamide(NIpAM) has been challenging with the problems of high dispersity, poor control over the molecular weights(MWs) or complex or multi reaction steps, etc. In this work, we report the well-controlled polymerization of NIpAM in water via a facile one-pot and one-step Cu(0)-mediated rDrp. The results of this approach show that the key for kicking off the Cu(0)-mediated NIpAM rDrps is to ensure sufficient Cu~I at the very beginning, and the key to achieve a well-controlled chain growth is to provide adequate deactivation strength during the polymerization process. For NIpAM, which has a high propagation rate constant, the deactivation control can be effectively enhanced by extra adding deactivator(i.e., Cu~II) to the system. Moreover, a low reaction temperature(4 ℃) is necessary in the controlled synthesis of higher MW poly(Nisopropylacrylamide)(pNIpAM) to avoid the compromise in control caused by the phase transition from its lower critical solution temperature(LCST). Through this new kinetically controlled strategy, pNIpAMs with well-defined structure, narrow molecular weight distributions(MWDs) and varied MWs were successfully achieved.

关键词： Cu(0)-mediated rDrp N-isopropylacrylamide propagation Deactivation reaction parameters

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Metal-organic nanosheet assembly ions sieving membrane for precise lithium ion and anion/solvent separation toward robust lithium metal battery

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Science china Chemistry 2024年第10期67卷 3320-3327页

作者： jian-Qiang Shen Tian-Tian Zhao ying-li Song lingjuan Zhang Ya-Nan Gao xiao-Nan Cui peng Zhao xian-Ming Zhang Key Laboratory of Magnetic Molecules & Magnetic Information Materials Ministry of EducationSchool of Chemistry & Material ScienceShanxi Normal University college of Chemistry and Chemical Engineering Taiyuan University of Technology

Metal-organic nanosheets（MONs） as a novel material with tunable pore structures and low mass transfer resistance,have emerged as molecular sieves for the separation of gases and *** theory,they can also serve as ion sieves for lithium metal batteries（LMBs）,realizing the high-energy and dendritic free ***,there are rarely relevant reports,because it is difficult to simultaneously balance efficient ion sieving ability,high ion passing rate and high electrochemical ***,we synthesized a stable ultrathin MON[Zn2（Bim）4]([Zn2（Bim）4]Nanosheet,HBim=benzimidazolate),which can achieve both efficient lithium ion sieving ability,high lithium ion passing rate and high electrochemical stability at the same *** separator assembled by this MON exhibits high li+transfer number of 0.81 due to the accurate lithium ion and anion/solvent *** battery containing such separator shows high lithium ionic conductivity of 0.74 m S cm-1and low activation energy of 0.099 eV,which can be attributed to the nanometer level thickness and the ion sieving *** is more,we realized the application of MONs-based ion sieves in LMBs with intercalation cathodes for the first *** the liFepO4|li battery with as-assembled separator demonstrates improved Coulombic efficiency（>99%） and significantly extended cycling life（>1600 cycles） with 80% capacity retention.

关键词： lithium sieving membrane metal-organic nanosheets lithium metal battery lithium ion and anion separation lithium ion and solvent separation

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Essential oils improve nursery pigs'performance and appetite via modulation of intestinal health and microbiota

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Animal Nutrition 2024年第1期16卷 174-188页

作者： Bi-Chen Zhao Tian-Hao Wang jian Chen bai-Hao Qiu Ya-ru Xu Jin-Long li college of Veterinary Medicine Northeast Agricultural UniversityHarbin150030China Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine Northeast Agricultural UniversityHarbin150030China Key Laboratory of the provincial Education Department of Heilongjiang for Common Animal Disease prevention and Treatment Northeast Agricultural UniversityHarbin150030China

Optimal intestinal health and functionality are essential for animal health and performance,and simultaneously intestinal nutrient transporters and intestinal peptides are also involved in appetite and feed intake control *** the potential of essential oil(EO)in improving animal performance and improving feed palatability,we hypothesized that dietary supplementation of cinnamaldehyde and carvacrol could improve performance and appetite of nursery pigs by modulating intestinal health and ***(100 mg/kg),carvacrol(100 mg/kg),and their mixtures(including 50 mg/kg cinnamaldehyde and 50 mg/kg carvacrol)were supplemented into the diets of 240 nursery pigs for 42 d,and data related to performance were ***,the influence of EO on intestinal health,appetite and gut microbiota and their correlations were *** supplementation increased(prage daily gain(ADG)and average daily feed intake(ADFI)of piglets,and reduced(prrhea rates in nursery ***,EO increased(prption area and the abundance of tight junction proteins,and decreased(ppermeability and local *** terms of intestinal development and the mucus barrier,EO promoted intestinal development and increased(pr of goblet ***,we found that piglets in the EO-supplemented group had upregulated(pransporters and digestive enzymes in the intestine,which were significantly associated with daily gain and feed *** addition,EO supplementation somewhat improved appetite in nursery pigs,increased the diversity of the gut microbiome and the abundance of beneficial bacteria,and there was a correlation between altered bacterial structure and appetite-related *** findings indicate that EO is effective in promoting growth performance and nutrient absorption as well as in regulating appetite by improving intestinal health and ba

关键词： Nursery pig Essential oil Intestinal health Appetite Microbiota

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pathogen-induced methylglyoxal negatively regulates rice bacterial blight resistance by inhibiting OsCDr1 protease activity

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Molecular plant 2024年第2期17卷 325-341页

作者： Zheng-Wei Fu jian-Hui li xiang Gao Shi-Jia Wang Ting-Ting Yuan ying-Tang Lu State Key Laboratory of Hybrid rice College of Life SciencesRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430072China Key Laboratory of Biology and Genetic Improvement of OilCrops Ministry of Agriculture and Rural AffairsOilCrops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430062China

Xanthomonas oryzae ***(Xoo)causes bacterial blight(BB),a globally devastating disease of rice(Oryza sativa)that is responsible for significant crop *** and sugar metabolites are important for pathogen infection,providing energy and regulating events associated with defense responses;howev-er,the mechanisms by which they regulate such events in BB are *** an inevitable sugar metabolite,methylglyoxal(MG)is involved in plant growth and responses to various abiotic stresses,but the underlying mechanisms remain *** and how MG functions in plant biotic stress responses is almost completely ***,we report that the Xoo strain pXO99 induces OsWrKY62.1 to repress transcrip-tion of OsGLY Il genes by directly binding to their promoters,resulting in overaccumulation of *** negatively regulates rice resistance against pxo99:osglyll2 mutants with higher MG levels are more sus-ceptible to the pathogen,whereas OsGLYIl2-overexpressing plants with lower MG content show greater resistance than the wild *** of OsGLYll2 to prevent excessive MG accumulation confers broad-spectrum resistance against the biotrophic bacterial pathogens Xoo and Xanthomonas oryzae *** and the necrotrophic fungal pathogen rhizoctonia solani,which causes rice sheath *** evidence shows that MG reduces rice resistance against pXO99 through CONSTITUTIVE DISEASE rESISTANCE 1(OsCDr1).MGmodifies the Arg97 residue of OsCDr1to inhibit its aspartic protease activ-ity,which is essential for OsCDr1-enhanced *** together,these findings illustrate how Xoo promotes infection by hijacking a sugar metabolite in the host plant.

关键词： Oryza sativa bacterial blight OsWrKY62.1 methylglyoxal OsCDr1

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Microvesicles derived from mesenchymal stem cells inhibit acute respiratory distress syndrome-related pulmonary fibrosis in mouse partly through hepatocyte growth factor

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World Journal of Stem Cells 2024年第8期16卷 811-823页

作者： Qi-Hong Chen ying Zhang Xue Gu peng-Lei Yang Jun Yuan li-Na Yu jian-Mei Chen Department of Critical Care Medicine Jiangdu People’s Hospital of YangzhouJiangdu People’s Hospital Affiliated to Yangzhou UniversityYangzhou 225200Jiangsu ProvinceChina Department of Critical Care Medicine Jiangdu People’s Hospital of YangzhouYangzhou 225200Jiangsu ProvinceChina Institute of Translational Medicine Medical CollegeYangzhou UniversityYangzhou 225000Jiangsu ProvinceChina

BACKGrOUND pulmonary fibrosis is one of the main reasons for the high mortality rate among acute respiratory distress syndrome(ArDS)*** stromal cell-derived microvesicles(MSC-MVs)have been shown to exert antifibrotic effects in lung *** To investigate the effects and mechanisms of MSC-MVs on pulmonary fibrosis in ArDS mouse *** MSC-MVs with low hepatocyte growth factor(HGF)expression(siHGF-MSC-MVs)were obtained via lentivirus transfection and used to establish the ArDS pulmonary fibrosis mouse *** intubation,respiratory mechanics-related indicators were measured via an experimental small animal lung function *** of MSC-MVs in lung tissues was investigated by near-infrared live ***,western blotting,EliSA and other methods were used to detect expression of pulmonary fibrosis-related proteins and to compare effects on pulmonary fibrosis and fibrosis-related *** The MSC-MVs gradually migrated and homed to damaged lung tissues in the ArDS model *** with MSC-MVs significantly reduced lung injury and pulmonary fibrosis ***,low expression of HGF(siHGF-MSC-MVs)significantly inhibited the effects of MSC-MVs(ppared with the ArDS pulmonary fibrosis group,the MSC-MVs group exhibited suppressed expression of type I collagen antigen,type III collagen antigen,and the proteins transforming growth factor-βandα-smooth muscle actin,whereas the siHGF-MVs group exhibited significantly increased expression of these *** addition,pulmonary compliance and the pressure of oxygen/oxygen inhalation ratio were significantly lower in the MSC-MVs group,and the effects of the MSC-MVs were significantly inhibited by low HGF expression(all pproved lung ventilation functions and inhibited pulmonary fibrosis in ArDS mice partly via HGF mrNA transfer.

关键词： Microvesicles derived from mesenchymal stem cells Acute respiratory distress syndrome pulmonary fibrosis Hepatocyte growth factor Mesenchymal stromal cells

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The only purple-flower species in Actaea *** pollinated by a hornet

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Journal of plant Ecology 2024年第4期17卷 181-196页

作者： lin-Feng Qiu Jiu-Dong Zhang ying li xiao-ying liu Dan-Qing Zhang Long Huang Ya-peng Yang Shi-Yu Wang Yue-Yi li Zi-Wei Ma Jie Sui lin Wang xiao-Fen Che xian-Hua Tian Yi ren jian-Qiang Zhang National Engineering Laboratory for resource Development of Endangered Crude Drugs in Northwest china College of Life SciencesShaanxi Normal UniversityXi’an 710119China Key Laboratory of Medicinal plant resource and Natural pharmaceutical Chemistry of Ministry of Education Shaanxi Normal UniversityXi’an 710119China college of Biological and Environmental Engineering Xi’an UniversityXi’an 710065China

Examining the pollination biology of plant species is not only crucial for enhancing our understanding of their reproductive biology,but also essential for elucidating their adaptation and evolutionary ***,we investigated the breeding system and pollination biology of two closely related species in *** flower of *** is unique in the genus with purple and chartaceous(paper-like)sepals,fewer stamens with yellow anthers and purple *** three seasons of field observation and exclusion experiments,we determined that *** was primarily pollinated by a hornet species,Vespa bicolor,which also served as the most efficient *** contrast,*** was primarily pollinated by large *** purpurea exhibited a significantly higher cumulative nectar volume than ***,which could be a crucial factor in attracting ***.A control experiment further demonstrated that removing the nectar leaf(petal)significantly decreased the visiting frequency of *** system studies revealed that both species were self-compatible,yet they primarily underwent outcrossing in natural *** study presents a compelling case of possible pollinator shift in *** accompanied by morphological divergence.A more in-depth investigation of this system would offer crucial insights into the extent to which pollinators are involved in the plant speciation process and whether they contribute to reproductive isolation between closely related species.

关键词： Actaea purpurea Actaea japonica breeding system pollinator shift speciation Vespa bicolor

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