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小麦渗透胁迫诱导基因 TaM YBSM151的克隆与分析

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华北农学报 2014年第4期29卷 19-24页

作者：李孟军史占良郭进考石家庄市农林科学研究院、河北省小麦工程技术研究中心河北石家庄050041

为了探讨转录因子在小麦抗旱分子机制中的作用,利用Affymetrix小麦表达谱芯片对渗透胁迫下普通小麦(石麦15)根系和叶片诱导表达谱进行了分析。在表达谱芯片上筛选到1个渗透胁迫诱导表达的MYB转录因子EST序列(GenBank登录号:BJ264503)。... 详细信息

为了探讨转录因子在小麦抗旱分子机制中的作用,利用Affymetrix小麦表达谱芯片对渗透胁迫下普通小麦(石麦15)根系和叶片诱导表达谱进行了分析。在表达谱芯片上筛选到1个渗透胁迫诱导表达的MYB转录因子EST序列(GenBank登录号:BJ264503)。根据该EST序列设计引物筛选石麦15基因组BAC文库,获得1个含有该EST序列的BAC单克隆。以BAC单克隆质粒为模板,通过BAC测序克隆了小麦MYB转录因子基因(TaMYBSM151)及其5'侧翼序列。TaMYBSM151的开放读码框长936 bp,编码1个含有311个氨基酸的R1-MYB转录因子。TaMYBSM151基因包含3个外显子和2个内含子。在TaMYBSM151 5'侧翼序列中,预测出33个脱水响应相关的顺式作用元件。实时定量PCR结果表明,TaMYBSM151受渗透胁迫诱导表达,但在根和叶中表达模式完全不同。以上结果表明,TaMYBSM151可能为脱水响应基因。

关键词：小麦 MYB转录因子渗透胁迫诱导基因

Low‑Temperature Fabrication of Stable Black‑Phase CsPbI_(3)Perovskite Flexible Photodetectors Toward Wearable Health Monitoring

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Nano-Micro Letters 2025年第3期17卷 232-245页

作者： Yingjie Zhao Yicheng Sun Chaoxin Pei Xing Yin Xinyi li Yi Hao mengru Zhang meng Yuan Jinglin Zhou Yu Chen Yanlin Song College of Chemistry and Pingyuan Laboratory Zhengzhou UniversityZhengzhou 450001People’s Republic of China Key Laboratory of Green Printing Institute of ChemistryChinese Academy of SciencesBeijing 100190People’s Republic of China Key Laboratory of Bio‑Inspired Materials and Interfacial Science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190People’s Republic of China Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049People’s Republic of China

Flexible wearable optoelectronic devices fabricated fromorganic–inorganic hybrid perovskites significantly accelerate the developmentof portable energy,biomedicine,and sensing fields,but their poor thermal stabilityhinders further ***,all-inorganic perovskites possessexcellent thermal stability,but black-phase all-inorganic perovskite filmusually requires high-temperature annealing steps,which increases energy consumptionand is not conducive to the fabrication of flexible wearable *** this work,an unprecedented low-temperature fabrication of stable blackphaseCsPbI3perovskite films is demonstrated by the in situ hydrolysis reactionof diphenylphosphinic chloride *** released diphenyl phosphateand chloride ions during the hydrolysis reaction significantly lower the phasetransition temperature and effectively passivate the defects in the perovskitefilms,yielding high-performance photodetectors with a responsivity of 42.1 AW−1 and a detectivity of 1.3×10^(14)***,high-fidelity imageand photoplethysmography sensors are demonstrated based on the fabricated flexible wearable *** work provides a newperspective for the low-temperature fabrication of large-area all-inorganic perovskite flexible optoelectronic devices.

关键词： In situ hydrolyzation Low-temperature processing All-inorganic perovskite Flexible photodetectors Health monitoring

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基于进程代数的安全协议分析与验证

计算机科学 2003年第6期30卷 23-26页

作者：李梦君李舟军陈火旺国防科技大学计算机学院长沙410073

Process algebra is the theories for concurrency. Now the methods based on process algebra for the analysis and verification of security protocols becomes one of the important methods for the study of the security protocols. In the paper, we outline these methods, especially the symbolic trace analysis method the bisimulation method.

关键词：网络攻击安全协议进程代数计算机网络网络安全加密体系

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： meng Cong Jing-Jing Hu Yan Yu Xiao-li li Xiao-Ting Sun li-Ting Wang Xia Wu ling-Jie Zhu Xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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采空侧沿空掘巷锚注支护围岩变形规律研究

煤炭技术 2016年第11期35卷 75-78页

作者：孔国强宋天奇王军李俊孟中国矿业大学矿业工程学院江苏徐州221116

以新景矿80116综放工作面回风巷为背景,运用FLAC^(3D)软件分析巷道围岩变形破坏特征,研究了中空锚索注浆加固机理,设计了锚注加固支护方案并进行了工程应用,有效控制了巷道的围岩变形。

关键词：锚注支护加固机理矿压显现

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基于支持向量机的卷烟焦油预测

计算机工程与应用 2007年第9期43卷 234-236页

作者：王强陈英武李孟军国防科技大学信息系统与管理学院长沙410073

分析了卷烟焦油含量预测问题,提出了基于支持向量机的卷烟焦油含量预测方法。首先,介绍了支持向量回归估计的学习算法。其次,建立了基于支持向量机的卷烟焦油含量预测模型。然后,提出了卷烟焦油含量支持向量机预测的实现算法。最后给出... 详细信息

分析了卷烟焦油含量预测问题,提出了基于支持向量机的卷烟焦油含量预测方法。首先,介绍了支持向量回归估计的学习算法。其次,建立了基于支持向量机的卷烟焦油含量预测模型。然后,提出了卷烟焦油含量支持向量机预测的实现算法。最后给出了一个算例。实例表明,该方法能够根据烟叶中的化学成分的测量值来预测卷烟的焦油含量。

关键词：支持向量机卷烟焦油含量化学成分预测模型