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Artificial enzymes:learning from nature and beyond

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Science China(life Sciences) 2023年第2期66卷 421-422页

作者： jinghong li Department of Chemistry Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing 100084China

As Professor Ronald Breslow,a pioneer of Artificial Enzymes and Biomimetic Chemistry,wrote in Artificial Enzymes,artificial enzymes are part of the world of Biomimetic Chemistry,which especially refers to accelerating reactions imitating/similar to natural catalytic processes,with a context not limited to the substances of biology(Breslow,2005).

关键词： catalytic beyond artificial

Levels of soluble delta-like ligand 1 in the serum and cerebrospinal fluid of tuberculous meningitis patients

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Neural Regeneration Research 2012年第11期7卷 874-878页

作者： jinghong li Jinyi li Yanjie Jia Department of Neurology First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 Henan Province China

In this study, the levels of soluble delta-like ligand 1 in cerebmspinal fluid and serum of 50 patients with tuberculous meningitis, 30 patients with viral meningitis, 20 patients with purulent meningitis and 40 subjects without central nervous system disease were determined using an enzyme-linked immunosorbent assay. The mean levels of soluble delta-like ligand 1 in both cerebrospinal fluid and serum from patients with tuberculous meningitis were significantly higher compared with those from patients with viral meningitis or purulent meningitis or from subjects without central nervous system disease. Meanwhile, the level of soluble delta-like ligand 1 gradually decreased as tuberculous meningitis patients recovered. If patients deteriorated after treatment, the level of soluble delta-like ligand 1 in cerebrospinal fluid gradually increased. There was no correlation between the level of soluble delta-like ligand 1 and the protein level/cell number in cerebrospinal fluid. Our findings indicate that the levels of soluble delta-like ligand 1 in cerebrospinal fluid and serum are reliable markers for the diagnosis of tuberculous meningitis and for monitoring treatment progress. At the same time, this index is not influenced by protein levels or cell numbers in cerebrospinal fluid.

关键词： delta-like ligand 1 cerebrospinal fluid enzyme linked immunosorbent assay tuberculous meningitis

Low temperature synthesis of NiO/Co_3O_4 composite nanosheets as high performance li-ion battery anode materials

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Chinese Science Bulletin 2012年第32期57卷 4195-4198页

作者： liANG QingQin li YueMing li jinghong Department of Chemistry Tsinghua University Beijing 100084China State Key Laboratory of Metastable Materials Science and Technology Yanshan UniversityQinhuangdao 066004China

NiO/Co3O4 composite nanosheets have been synthesized via a facile method at low temperature for the first *** as prepared materials were characterized by X-ray powder diffraction(XRD) and transmission electron microscopy(TEM),and the performance of li-ion batteries(liBs) as anode materials were also *** controlling the atom ratio of Ni:Co,not only the size of the nanosheets can be controlled,the electrode's conductivity and stability could also be greatly *** composite material showed a stable capacity retention during cycling(87% of the second capacity was retained after 15 cycles) even at a relatively large current rate(400 mA/g).The NiO/Co3O4 nanosheet might be promising candidate anode materials in high performance li-ion batteries.

关键词：锂离子电池负极材料低温合成复合材料纳米性能 X-射线粉末衍射透射电子显微镜

Recent advances in enzyme-free and enzyme-mediated single-nucleotide variation assay in vitro

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National Science Review 2024年第5期11卷 365-384页

作者： Erhu Xiong Pengfei liu Ruijie Deng Kaixiang Zhang Ronghua Yang jinghong li Key Laboratory of Chemical Biology &Traditional Chinese Medicine Research Ministry of EducationCollege of Chemistry and Chemical Engineering Hunan Normal University College of Biomass Science and Engineering Healthy Food Evaluation Research CenterSichuan University School of Pharmaceutical Sciences Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases Zhengzhou University Department of Chemistry Center for Bioanalytical Chemistry Key Laboratory of Bioorganic Phosphorus Chemistry& Chemical BiologyTsinghua University Beijing Institute of life Science and Technology

Single-nucleotide variants(SNVs) are the most common type variation of sequence alterations at a specific location in the genome, thus involving significant clinical and biological information. The assay of SNVs has engaged great awareness, because many genome-wide association studies demonstrated that SNVs are highly associated with serious human diseases. Moreover, the investigation of SNV expression levels in single cells are capable of visualizing genetic information and revealing the complexity and heterogeneity of single-nucleotide mutation-related diseases. Thus, developing SNV assay approaches in vitro, particularly in single cells, is becoming increasingly in demand. In this review, we summarized recent progress in the enzyme-free and enzyme-mediated strategies enabling SNV assay transition from sensing interface to the test tube and single cells, which will potentially delve deeper into the knowledge of SNV functions and disease associations, as well as discovering new pathways to diagnose and treat diseases based on individual genetic profiles. The leap of SNV assay achievements will motivate observation and measurement genetic variations in single cells, even within living organisms, delve into the knowledge of SNV functions and disease associations, as well as open up entirely new avenues in the diagnosis and treatment of diseases based on individual genetic profiles.

关键词： single-nucleotide variant point mutation in vitro enzyme-free assay enzyme-mediated assay

Real-time identification of multiple nanoclusters with a protein nanopore in single-cluster level

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Nano Research 2024年第1期17卷 262-269页

作者： ling Zhang Peilei He Huang Chen Qingda liu libo li Xun Wang jinghong li Department of Chemistry Center for BioAnalytical ChemistryKey Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing 100084China School of Chemistry and Chemical Engineering Guangdong Prov Key Lab Green Chem Prod TechnolSouth China University of TechnologyGuangzhou 510640China New Cornerstone Science Laboratory Shenzhen 518054China Beijing Institute of life Science and Technology Beijing 102206China Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at MicroscaleUniversity of Science and Technology of ChinaHefei 230026China

It is important and challenging to analyze nanocluster structure with atomic ***,α-hemolysin nanopore was used to identify nanoclusters at the single molecule level by providing two-dimensional(2D)dwell time–current blockage spectra and translocation event frequency which sensitively depended on their *** such as Anderson,Keggin,Dawson,and a few lacunary Dawson polyoxometalates with very similar structures,even with only a two-atom difference,could be *** nanopore device could simultaneously measure multiple nanoclusters in a mixture qualitatively and ***,molecular dynamics(MD)simulations provided microscopic understandings of the nanocluster translocation dynamics and yielded 2D dwell time–current blockage spectra in close agreement with *** nanopore platform provides a novel powerful tool for nanocluster characterization.

关键词： nanopore single-molecule nanocluster polyoxometalates real-time detection

CuO/Cu2O nanowire array photoelectrochemical biosensor for ultrasensitive detection of tyrosinase

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Science China Chemistry 2020年第7期63卷 1012-1018页

作者： Xiaoxi Guo Jinghua Wu lian Xia Meihao Xiang Fengli Qu jinghong li College of Chemistry and Chemical Engineering Qufu Normal UniversityQufu 273165China Department of Chemistry Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing 100084China

Photoelectrochemical(PEC) biosensors have shown great promise in bioanalysis and diagnostic applications in recent years. In this work, the CuO/Cu2O nanowire array(CuO/Cu2O Nanowire) supported on copper foam was prepared as a photocathode for detection of tyrosinase though quinone-chitosan conjugation chemistry method. The in-situ generated quinones that were the catalytic product of tyrosinase acted as electron acceptors, which were captured by the chitosan deposited on the surface of the electrode. Direct immobilization of electron acceptor on the electrode surface improved the photocurrent conversion efficiency and thus sensitivity. The as-prepared biosensor can realize a rapid response in a wide linear range of 0.05 U/mL to 10 U/mL with the detection limit as low as 0.016 U/mL of tyrosinase. The current work provides a new perspective to design and develop highly sensitive and selective PEC biosensor.

关键词： tyrosinase Cu0/Cu20 nanowire photoelectrochemical biosensor chitosan modified electrode quinones

Metal chelate affinity to immobilize horseradish peroxidase on functionalized agarose/CNTs composites for the detection of catechol

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Science China Chemistry 2011年第8期54卷 1319-1326页

作者： TU XinMan LUO Shenglian LUO XuBiao ZHAO YingJie FENG li li jinghong Key Laboratory of Jiangxi Province for Ecological Diagnosis-Remediation and Pollution Control School of Environment and Chemical Engineering Nanchang Hangkong University Nanchang 330063 China Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology Ministry of Education Department of Chemistry Tsinghua University Beijing 100084 China

The paper reports a novel amperometric biosensor for catechol based on immobilization of a highly sensitive horseradish peroxidase by affinity interactions on metal chelate-functionalized agarose/carbon nanotubes composites. Metal chelate affinity takes advantage of the affinity of Ni2＋ ions to bind strongly and reversibly to histidine or cysteine tails found on the surface of the horseradish peroxidase. Thus, enzymes with such residues in their molecules can be easily attached to functionalized aga- rose/carbon nanotubes composites support containing a nickel chelate. linear sweep voltammograms and amperometry are used to study the proposed electrochemical biosensor. Catechol is determined by direct reduction of biocatalytically liberated quinone species at -0.05 V （vs. SCE）. The effect ofpH, applied electrode potential and the concentration of H2O2 on the sensitivity of the biosensor has been investigated. The performance of the proposed biosensor is tested using four different phenolic compounds, showing very high sensitivity, in particular, the linearity of cateehol is observed from 2.0 × 10-8 to 1.05×10-5 M with a detection limit of 5.0×10-9 M.

关键词： amperometric biosensor carbon nanotubes metal chelation agarose catechol

Simultaneous capture of ISG15 conjugating and deconjugating enzymes using a semi-synthetic ISG15-Dha probe

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Science China Chemistry 2023年第3期66卷 837-844页

作者： Chuntong li Tian Wang Lujun liang Guochao Chu Jiachen Zhang Wei He Lei liu jinghong li Department of Chemistry Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyCenter for Synthetic and Systems BiologyState Key Laboratory of Chemical Oncogenomics(Shenzhen)Tsinghua UniversityBeijing 100084China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at MicroscaleUniversity of Science and Technology of ChinaHefei 230026China

ISG15 is a ubiquitin-like(Ubl) protein attached to substrate proteins by ISG15 conjugating enzymes whose dysregulation is implicated in a multitude of disease processes, but the probing of these enzymes remains to be accomplished. Here, we describe the development of a new activity-based probe ISG15-Dha(dehydroalanine) through protein semi-synthesis. In vitro crosslinking and cell lysate proteomic profiling experiments showed that this probe can sequentially capture ISG15 conjugating enzymes including E1 enzyme UBA7, E2 enzyme UBE2L6, E3 enzyme HERC5, the previously known ISG15 deconjugating enzyme(USP18), as well as some other enzymes(USP5 and USP14) which we additionally confirmed to impart deISGylation activity. Collectively, ISG15-Dha provides a new tool that can simultaneously capture ISG15 conjugating and deconjugating enzymes for biochemical or pharmacological studies.

关键词： ISG15-Dha probe semi-synthesis activity based probe proteomics ISGylation

Single-Molecule Bioelectronic Sensors with AI-Aided Data Analysis:Convergence and Challenges

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Precision Chemistry 2024年第10期2卷 518-538页

作者： Yuxin Yang Yueqi li Longhua Tang jinghong li State Key Laboratory of Extreme Photonics and Instrumentation College of Optical Science and EngineeringZhejiang UniversityHangzhou 310027China Nanhu Brain-Computer Interface Institute HangzhouZhejiang 311100China Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at MicroscaleUniversity of Science and Technology of ChinaHefei 230026China Department of Chemistry Center for BioAnalytical ChemistryKey Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing 100084China Beijing Institute of life Science and Technology Beijing 102206ChinaNew Cornerstone Science InstituteBeijing 102206China

Single-molecule bioelectronic sensing,a groundbreaking domain in biological research,has revolutionized our understanding of molecules by revealing deep insights into fundamental biological *** advent of emergent technologies,such as nanogapped electrodes and nanopores,has greatly enhanced this field,providing exceptional sensitivity,resolution,and integration ***,challenges persist,such as complex data sets with high noise levels and stochastic molecular *** intelligence(AI)has stepped in to address these issues with its powerful data processing *** algorithms effectively extract meaningful features,detect subtle changes,improve signal-to-noise ratios,and uncover hidden patterns in massive *** review explores the synergy between AI and single-molecule bioelectronic sensing,focusing on how AI enhances signal processing and data analysis to boost accuracy and *** also discuss current limitations and future directions for integrating AI,highlighting its potential to advance biological research and technological innovation.

关键词： single-molecule bioelectronic sensing artificial intelligence machine learning signal processing biosensors