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Genome-wide analyses on transcription factors and their potential microRNA regulators involved in maize male fertility

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The Crop Journal 2021年第6期9卷 1248-1262页

作者： Ziwen li Taotao Zhu Shuangshuang liu Yilin Jiang haoyun liu Yuwen Zhang Ke Xie Jinping li Xueli An Xiangyuan Wan Zhongzhi International Institute of Agricultural Biosciences Biology and Agriculture Research CenterUniversity of Science and Technology BeijingBeijing 100024China Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding Beijing International Science and Technology Cooperation Base of Bio-Tech BreedingBeijing 100192China

Anther development is a programmed biological process crucial to plant male reproduction. Genomewide analyses on the functions of transcriptional factor(TF) genes and their microRNA(miRNA) regulators contributing to anther development have not been comprehensively performed in maize. Here, using published RNA-Seq and small RNA-Seq(sRNA-Seq) data from maize anthers at ten developmental stages in three genic male-sterility(GMS) mutants(ocl4, mac1, and ms23) and wild type W23, as well as newly sequenced maize anther transcriptomes of ms7-6007 and lob30 GMS mutants and their WT lines, we analyzed and found 1079 stage-differentially expressed(stage-DE) TF genes that can be grouped into six(premeiotic, meiotic, postmeiotic, premeiotic-meiotic, premeiotic-postmeiotic, and meiotic-postmeiotic clusters) expression clusters. Functional enrichment combined with cytological and physiological analyses revealed specific functions of genes in each expression cluster. In addition, 118 stage-DE miRNAs and99 miRNA-TF gene pairs were identified in maize anthers. Further analyses revealed the regulatory roles of zma-miR319 and zma-miR159 as well as ZmMs7 and ZmLOB30 on ZmGAMYB expression. Moreover,ZmGAMYB and its paralog ZmGAMYB-2 were demonstrated as novel maize GMS genes by CRISPR/Cas9 knockout analysis. These results extend our understanding on the functions of miRNA-TF gene regulatory pairs and GMS TF genes contributing to male fertility in plants.

关键词： Transcription factor gene microRNA Anther development Male fertility Genic male sterility

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Ferroelectricity coexisted with p-orbital ferromagnetism and metallicity in two-dimensional metal oxynitrides

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npj Computational Materials 2022年第1期8卷 581-587页

作者： Haoqiang Ai Feifei li haoyun Bai Dong liu Kin Ho Lo Shengyuan AYang Yoshiyuki Kawazoe Hui Pan Department of Electromechanical Engineering Faculty of Science and TechnologyUniversity of Macao999078TaipaMacao SARP.R.China Institute of Applied Physics and Materials Engineering University of Macao999078TaipaMacao SARP.R.China Research Laboratory for Quantum Materials Singapore University of Technology and DesignSingapore487372Singapore Center for Quantum Transport and Thermal Energy Science School of Physics and TechnologyNanjing Normal University210023NanjingP.R.China New Industry Creation Hatchery Center Tohoku UniversitySendai980-8579Japan Department of Physics and Nanotechnology SRM Institute of Science and TechnologyKattankulathur603203Tamil NaduIndia School of Physics Suranaree University of Technology111 University Avenue MuangNakhon Ratchasima30000Thailand Department of Physics and Chemistry Faculty of Science and TechnologyUniversity of Macao999078TaipaMacao SARP.R.China

Two-dimensional (2D) multiferroics have attracted increasing interests in basic science and technological fields in recent ***,most reported 2D magnetic ferroelectrics are based on the d-electron magnetism,which makes them rather rare due to the empirical d^(0) rule and limits their applications for low magnetic phase transition *** this work,we demonstrate that the ferroelectricity can coexist with the p-electron-induced ferromagnetism without the limitation of d^(0) rule and metallicity in a family of stable 2D MXene-analogous oxynitrides,X_(2)NO_(2) (X = In,Tl).Remarkably,the itinerant character of p electrons can lead to the strong ferromagnetic metallic ***,a possible magnetoelectric effect is manifested in a Tl_(2)NO_(2)/WTe_(2) heterostructure through the interface *** findings provide an alternative possible route toward 2D multiferroics and enrich the concept of ferroelectric metals.

关键词： metallic ferroelectric dimensional

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High-speed impulsive stimulated Brillouin microscopy

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Photonics Research 2024年第4期12卷 730-739页

作者： JIARUI li TAORAN LE HONGYUAN ZHANG haoyun WEI YAN li State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision InstrumentTsinghua UniversityBeijing 100084China Cole Eye Institute Cleveland ClinicClevelandOhio 44195USA

Brillouin microscopy, which maps the elastic modulus from the frequency shift of scattered light, has evolved to a faster speed for the investigation of rapid biomechanical changes. Impulsive stimulated Brillouin scattering (ISBS)spectroscopy has the potential to speed up measurement through the resonant amplification interaction from pulsed excitation and time-domain continuous detection. However, significant progress has not been achieved due to the limitation in signal-to-noise ratio (SNR) and the corresponding need for excessive averaging to maintain high spectral precision. Moreover, the limited spatial resolution also hinders its application in mechanical imaging. Here, by scrutinizing the SNR model, we design a high-speed ISBS microscope through multi-parameter optimization including phase, reference power, and acquisition time. Leveraging this, with the further assistance of the Matrix Pencil method for data processing, three-dimensional mechanical images are mapped under multiple contrast mechanisms for a millimeter-scale polydimethylsiloxane pattern immersed in methanol, enabling the identification of these two transparent materials without any contact or labeling. Our experimental results demonstrate the capability to maintain high spectral precision and resolution at a sub-millisecond integration time for one pixel. With a two-order improvement in the speed and a tenfold improvement in the spatial resolution over the state-of-the-art systems, this method makes it possible for ISBS microscopes to sensitively investigate rapid mechanical changes in time and space.

关键词： Brillouin maintain methanol

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电力电容器现场维修更换装置

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电力电容器与无功补偿 2024年第2期45卷 1-5页

作者：杨枝林李皓昀颜红艳莫国平王增文黄楚秋桂林电力电容器有限责任公司广西桂林541004

本文介绍了一种用于变电站、换流站等场所电力电容器现场维修更换装置,通过对装置总体设计方案、具体的结构及现场使用的详细描述,明确了电容器更换装置结构及使用要求。应用证明:该装置能安全有效地更换电力电容器成套装置上的电容器,... 详细信息

本文介绍了一种用于变电站、换流站等场所电力电容器现场维修更换装置,通过对装置总体设计方案、具体的结构及现场使用的详细描述,明确了电容器更换装置结构及使用要求。应用证明:该装置能安全有效地更换电力电容器成套装置上的电容器,可较好地解决上述电力电容器成套装置的电容器维修问题和困难。

关键词：电容器更换装置维修提升机旋转翼卷扬机电力电容器

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Adaptive cavity-enhanced dual-comb spectroscopy

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Photonics Research 2019年第8期7卷 883-889页

作者： WEIPENG ZHANG XINYI CHEN XUEJIAN WU YAN li haoyun WEI State Key Laboratory of Precision Measurement Technology&Instruments Department of Precision InstrumentTsinghua UniversityBeijing100084China Department of Physics 366Le Conte Hall MS7300University of CaliforniaBerkeleyCalifornia94720USA

Resolution and bandwidth are critical for cavity-enhanced dual-comb spectroscopy(CE-DCS). Here, we pioneer an adaptive approach in CE-DCS to improve the broadband as well as the resolution. Postcorrections to dualcomb interferograms adaptively compensate the relative phase jitters of the optical frequency combs and result in both a mode-resolved spectral resolution and a signal-to-noise ratio of 440:1 in 1 s. Meanwhile, an adaptive combcavity locking scheme exploits more than 90% of the comb modes, covering 340 cm-1(10 THz) at 6450 cm-1. For a single dual-comb interferogram, more than 40,000 comb teeth spaced by 250 MHz are measured in less than7.5 ms, contributing to a noise equivalent absorption per spectral element of 2 × 10-10 cm-1·Hz-1∕2. This adaptive cavity-enhanced dual-comb spectroscopy technique provides an attractive spectroscopic tool that may be utilized in trace-gas sensing, breath and cancer analysis, and engine combustion diagnosis.

关键词： DCS CW Adaptive cavity-enhanced dual-comb spectroscopy

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3GHz边耦合直线加速结构单元腔优化设计

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现代应用物理 2023年第1期14卷 97-102页

作者：李皓云苗凯杨少华余长益黄帅王睿宋祎鹏杨松易俗冉敬国彭涛四川红华实业有限公司四川乐山614000

利用CST studio suite程序对边耦合直线加速结构的加速腔及耦合腔进行建模仿真,并进行优化。加速腔结构由11个参数决定,优化过程需平衡多个参数,包括本征模式、高频特性、最大表面电场、有效分路阻抗及渡越时间因子;耦合腔的优化基于维... 详细信息

利用CST studio suite程序对边耦合直线加速结构的加速腔及耦合腔进行建模仿真,并进行优化。加速腔结构由11个参数决定,优化过程需平衡多个参数,包括本征模式、高频特性、最大表面电场、有效分路阻抗及渡越时间因子;耦合腔的优化基于维持本征模式3 GHz。结果表明,在26~120 MeV整个加速过程中,加速腔的有效分路阻抗为22.5~59.8 MΩ·m^(-1),加速梯度为11~14.7 MV·m^(-1)。

关键词：质子束流边耦合加速结构加速腔耦合腔

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The role of corneal endothelium in macular corneal dystrophy development and recurrence

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Science China(life Sciences) 2024年第2期67卷 332-344页

作者： Bi-Ning Zhang Benxiang Qi Chunxiao Dong Bin Zhang Jun Cheng Xin Wang Suxia li Xiaoyun Zhuang Shijiu Chen haoyun Duan Dewei li Sujie Zhu Guoyun li Yihai Cao Qingjun Zhou lixin Xie Eye Institute of Shandong First Medical University Qingdao Eye Hospital of Shandong First Medical UniversityQingdao 266071China State Key Laboratory Cultivation Base Shandong Provincial Key Laboratory of OphthalmologyQingdao 266071China School of Ophthalmology Shandong First Medical UniversityQingdao 250021China Department of Medicine Qingdao UniversityQingdao 266071China Eye Institute of Shandong First Medical University Eye Hospital of Shandong First Medical University(Shandong Eye Hospital)Jinan 250021China Department of Ophthalmology School of Clinical MedicineWeifang Medical UniversityWeifang 261072China Institute of Translational Medicine College of MedicineQingdao UniversityQingdao 266071China Key Laboratory of Marine Drugs of Ministry of Education Shandong Provincial Key Laboratory of Glycoscience and GlycotechnologySchool of Medicine and PharmacyOcean University of ChinaQingdao 266071China Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China Department of Microbiology Tumor and Cell BiologyBiomedicumKarolinska InstitutetStockholm 17177Sweden

Macular corneal dystrophy(MCD)is a progressive,bilateral stromal dystrophic disease that arises from mutations in carbohydrate sulfotransferase 6(CHST6).Corneal transplantation is the ultimate therapeutic solution for MCD ***,postoperative recurrence remains a significant *** conducted a retrospective review of a clinical cohort comprising 102 MCD patients with 124 eyes that underwent either penetrating keratoplasty(PKP)or deep anterior lamellar keratoplasty(DALK).Our results revealed that the recurrence rate was nearly three times higher in the DALK group(39.13%,9/23 eyes)compared with the PKP group(10.89%,11/101 eyes),suggesting that surgical replacement of the corneal endothelium for treating MCD is advisable to prevent postoperative *** experimental data confirmed the robust m RNA and protein expression of CHST6 in human corneal endothelium and the rodent homolog CHST5 in mouse *** knockdown of wild-type Chst5 in mouse corneal endothelium(AC^(siChst5)),but not in the corneal stroma,induced experimental MCD with similar extracellular matrix synthesis impairments and corneal thinning as observed in MCD *** carrying Chst5 point mutation also recapitulated clinical phenotypes of MCD,along with corneal endothelial *** injection of wild-type Chst5 rescued the corneal impairments in AC^(siChst5)mice and retarded the disease progression in Chst5 mutant ***,our study provides new mechanistic insights and therapeutic approaches for MCD treatment by highlighting the role of corneal endothelium in MCD development.

关键词： macular corneal dystrophy recurrence corneal endothelium keratan sulfate penetrating keratoplasty

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Interpulse stimulation Fourier-transform coherent anti-Stokes Raman spectroscopy

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Photonics Research 2023年第2期11卷 357-363页

作者： MINJIAN LU YUJIA ZHANG XINYI CHEN YAN li haoyun WEI Department of Precision Instrument State Key Laboratory of Precision Measurement Technology and InstrumentsTsinghua UniversityBeijing 100084China

Exploiting the time-resolving ability of ultrafast pulses, Fourier-transform coherent anti-Stokes Raman scattering(FT-CARS) stands out among the coherent Raman spectroscopic techniques for providing high-speed vibrational spectra with high spectral resolution, high Raman intensity, and immunity to nonresonant background. However,the impulsive stimulation nature of FT-CARS imposes heavy demands on the laser source and makes it inherently difficult to monitor high-frequency vibrations. Here, a novel FT-CARS strategy to our knowledge based on interpulse stimulation is proposed to provide more flexible measuring wavenumber region and lighten the requirement on ultrafast pulses. The mechanism of this technique is analyzed theoretically, and simulation is performed to show an orders-of-magnitude improvement of Raman intensity in the high-wavenumber region by the ***, an ytterbium-doped fiber laser and photonic crystal fiber-based solitons are employed to provide two~100-fs pulses as the pump and Stokes, respectively, and to perform interpulse stimulation FT-CARS without sophisticated dispersion control devices. The high-wavenumber region and upper-part fingerprint region measurements are demonstrated as examples of flexible measurement. Combined with other rapid scanning techniques, such as resonant scanners or a dual-comb scheme, this interpulse stimulation FT-CARS promises to make the fascinating FT-CARS available for any desired wavenumber region, covering many more realistic scenarios for biomedical, pathological, and environmental research.

关键词： coherent resonant vibrational

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短纤维插层碳纤维/环氧树脂复合材料层间性能

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复合材料学报 2022年第8期39卷 3674-3683页

作者：郑昊李岩涂昊昀同济大学航空航天与力学学院上海200092

碳纤维/环氧树脂复合材料因具有高的比强度、比模量、抗疲劳性好等优点,已在航空航天、轨道交通、汽车等领域得到越来越广泛的应用。然而作为碳纤维/环氧树脂复合材料的主要结构形式之一的层合板结构,由于厚度方向没有纤维,导致层间性... 详细信息

碳纤维/环氧树脂复合材料因具有高的比强度、比模量、抗疲劳性好等优点,已在航空航天、轨道交通、汽车等领域得到越来越广泛的应用。然而作为碳纤维/环氧树脂复合材料的主要结构形式之一的层合板结构,由于厚度方向没有纤维,导致层间性能最弱,极易发生分层破坏,影响结构的承载性能。短纤维层间插层增韧是改善碳纤维/环氧树脂复合材料层间性能的有效手段,近年来,国内外学者开展了大量的相关研究工作,但仍缺乏短纤维层间插层增韧的系统研究。本论文选用碳纤维、亚麻纤维及Kevlar纤维作为插层短纤维,系统地分析和讨论了短纤维种类、插层面密度和纤维长度对碳纤维/环氧树脂复合材料层间增韧效果的影响。结果表明,对于不同的短纤维插层,均存在着随着短纤维长度和插层面密度的增加,复合材料层间断裂韧性先上升后下降的趋势。在此基础上揭示了不同种类纤维的增韧机制,纤维桥联、裂纹扩展路径改变、纤维多层级破坏及纵向撕裂等均有助于复合材料层间断裂韧性的提高,研究结果为短纤维插层增韧复合材料的结构设计奠定了基础。

关键词：碳纤维复合材料断裂韧性层间增韧短纤维插层纤维桥联力学性能

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过采样型傅里叶红外光谱仪的数字滤波方法

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红外与激光工程 2012年第6期41卷 1438-1441页

作者：任利兵尉昊赟李岩清华大学精密仪器与机械学系精密测试技术及仪器国家重点实验室北京100084

傅里叶红外光谱仪使用的红外探测器频率响应范围通常为几十Hz到数MHz,这使得探测器直接接收的干涉信号中很容易混入环境噪声,引起真实干涉信号的畸变。过采样型傅里叶红外光谱仪等同于将传统光谱仪的等光程间隔采样过程"搬移"到计算机内... 详细信息

傅里叶红外光谱仪使用的红外探测器频率响应范围通常为几十Hz到数MHz,这使得探测器直接接收的干涉信号中很容易混入环境噪声,引起真实干涉信号的畸变。过采样型傅里叶红外光谱仪等同于将传统光谱仪的等光程间隔采样过程"搬移"到计算机内,因此,理论上允许使用数字滤波器代替模拟滤波器对红外干涉信号降噪。在分析过采样型傅里叶红外光谱仪红外干涉信号有效频率范围的基础上,详述了适用于过采样型傅里叶红外光谱仪的巴特沃斯数字带通滤波器的设计方法,并应用设计的滤波器对过采样的红外干涉信号进行了降噪研究。结果表明:数字滤波的方法能有效去除红外干涉信号中的高、低频噪声,保留真实的光谱信号,在使用上也比模拟滤波更加灵活、便捷。

关键词：傅里叶红外光谱仪数字滤波过采样噪声

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