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Effect of in ovo folic acid injection on hepatic IGF2 expression and embryo growth of broilers

Journal of Animal Science and Biotechnology 2017年第1期8卷 196-204页

作者： Yanli liu lihui Zhi jing Shen Shizhao li Junhu Yao Xiaojun Yang College of Animal Science and Technology Northwest A&F University School of Mathematics and Computer Science Shan Xi Normal University

Background： Insulin-like factor 2（IGF2） plays an important role in embryonic growth process by modulating intermediary metabolism and cell proliferation. Folic acid is involved in one carbon metabolism and contributes to DNA methylation which is related to gene expression. The purpose of this study was to explore whether folic acid could regulate IGF2 expression via epigenetic mechanism and further promote embryonic growth of new-hatched ***： In the present study, 360 fertile eggs were selected and randomly assigned to four treatments. On11 embryonic day of incubation（E11）, 0, 50, 100 and 150 μg folic acid were injected into eggs *** hatched, growth performance of broilers were calculated. Hepatic IGF2 expression, methylation level and chromatin structure of promoter region were ***： Results have showed that IGF2 expression was up-regulated in 150 μg folic acid group（P 〈 0.05） and other two dose of folic acid did not affect gene expression（P 〉 0.05）. Meanwhile, methylation level of IGF2 promoter were lower in 100 and 150 μg groups, which was consistent with lower expression of DNA methyltransferase1（DNMT1）（P 〈 0.05）. What＇s more, chromatin looseness of IGF2 promoter was higher in 150 μg group than control group（P 〈 0.05）. Further, birth weight（BW）, liver and bursa index of new-hatched chickens in 150 μg folic acid group were higher than the other groups（P 〈 0.05）. There were positive correlations between hepatic IGF2 expression and BW and organs index（P 〈 0.05）.Conclusion： In conclusion, our data have demonstrated that 150 μg folic acid injection on E11 could up-regulate IGF2 expression by modulating DNA hypomethylation and improving chromatin accessibility in the gene promoter region,and ulteriorly facilitate embryonic growth and organ development of broilers.

关键词： Broiler Chromatin structure Folic acid Insulin-like growth factor 2 Methylation

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Characterization and Phylogenetic Analysis of Lectin Gene cDNA Isolated from Sea Cucumber (Apostichopus japonicus) Body Wall

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Journal of Ocean University of China 2017年第6期16卷 1148-1156页

作者： XUE Zhuang li Hui liU Yang ZHOU Wei SUN jing WANG Xiuli College of Fisheries and life Science Dalian Ocean University Dalian 116023 P. R. China liaoning Province Academy of Analytic Science Shenyang 110015 P. R. China

As a ‘living fossil' of species origin and ‘rich treasure' of food and nutrition development, sea cucumber has received a lot of attentions from researchers. The cDNA library construction and EST sequencing of blood had been conducted previously in our lab. The bioinformatic analysis provided a gene fragment which is highly homologous with the genes of lectin family, named AjL(Apostichopus japonicus lectin). To characterize and determine the phylogeny of AjL genes in early evolution, we isolated a fulllength cDNA of lectin gene from the body wall of A. japonicus. The open reading frame of this gene contained 489 bp and encoded a 163 amino acids secretory protein being homologous to lectins of mammals and aquatic organisms. The deduced protein included a lectin-like domain. SDS-PAGE analysis showed that AjL migrated as a specific band(about 36.09 kDa under reducing), and agglutinated against rabbit red blood cells. AjL was similar to chain A of CEL-IV in space structure. We predicted that AjL may play the same role of CEL-IV. Our results suggested that more than one lectin gene functioned in sea cucumber and most of other species, which was fused by uncertain sequences during the evolution and encoded different proteins with diverse functions. Our findings provided the insights into the function and characteristics of lectin genes invertebrates. The results will also be helpful for the identification and structural, functional, and evolutionary analyses of lectin genes.

关键词： sea cucumber agglutination activity lectin space structure

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Microstructure and Transformation Characteristics of Acicular Ferrite in High Niobium-Bearing Microalloyed Steel

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Journal of Iron and Steel Research International 2010年第6期17卷 53-59页

作者： YANG jing-hong liU Qing-you SUN Dong-bai li Xiang-yang Luoyang Ship Material Research Institute Luoyang 471039 Henan China China Iron and Steel Research Institute Group Beijing 100081 China School of Materials Seienee and Engineering University of Seienee and Technology Beijing 10083 China

The transformation behavior and microstructural characteristics of a low carbon high niobium-bearing microalloyed pipeline steel were investigated by deformation dilatometry and microstructure observation. The continuous cooling transformation curves of the test steel were constructed. The results showed that high niobium content and deformation enhanced the formation of acicular ferrite; the microstructures changed from polygonal ferrite, quasi-polygonal ferrite to acicular ferrite with increasing cooling rates from 0.5 to 50 ℃/s and was dominated by acicular ferrite in a broadened cooling rate higher than 5 ℃/s. The chaotic microstructure consisted of non-equiaxed ferrite and interwoven ferrite laths with high density dislocations and subunits. The results of isothermal holding treatment showed that acicular ferrite microstructure was formed at 550-600 ℃ and consisted of highly misoriented plate packets having internal low angle boundaries. With increasing the holding time or temperature,some low misorientation boundaries changed to high misorientation owing to the movement of dislocations and coarsening of grain.

关键词： microalloyed steel acicular ferrite transformation continuous cooling transformation

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Visualization Detection of Solid-liquid Two-Phase Flow in Filling Pipeline by Electrical Capacitance Tomography Technology

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Computer Modeling in Engineering & Sciences 2022年第4期131卷 465-476页

作者： Ningbo jing Mingqiao li Lang liu Yutong Shen Peijiao Yang Xuebin Qin College of Electrical and Control Engineering Xi’an University of Science and TechnologyXi’an710054China College of Energy Engineering Xi’an University of Science and TechnologyXi’an710054China

During mine filling,the caking in the pipeline and the waste rock in the filling slurry may cause serious safety accidents such as pipe blocking or ***,the visualization of the innermine filling of the solid-liquid two-phase flow in the pipeline is *** paper proposes a method based on capacitance tomography for the visualization of the solid-liquid distribution on the section of a filling pipe.A feedback network is used for electrical capacitance tomography *** reconstruction method uses radial basis function neural network fitting to determine the relationship between the capacitance vector and medium distribution *** the reconstruction process,the error in the linear back projection is removed;thus,the reconstruction problem becomes an accurate linear *** simulation results showthat the reconstruction accuracy of this algorithm is better than that of many traditional algorithms;furthermore,the reconstructed image artifacts are fewer,and the phase distribution boundary is *** method can help determine the location and size of the caking and waste rock in the cross section of the pipeline more accurately and has great application prospects in the visualization of filling pipelines in mines.

关键词： Electrical capacitance tomography mine backfilling visualization detection image reconstruction radial basis function neural network

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Application of three Ln(Ⅲ)-coordination polymers in fields of luminescence,antibacteria and detection of Fe^(3+) and 4-nitrophenol

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Journal of Rare Earths 2023年第9期41卷 1392-1397,I0005页

作者： Lun Zhao Yue Han Guanlin Guo Helong Bai Zhexin Wang Hongwei jing Guanying Song Ziyun Wang Jiayu li Jia li College of Chemistry Changchun Normal UniversityChangchun 130032China

Three lanthanide-based coordination polymers(Ln-CPs) with layered structure,{[La_(2)(L)_(3)(DMF)(H_(2)O)_(3)]·H_(2)O}_n(1),{[Eu_(2)(L)_(3)(DMF)_(2)(H_(2)O)]·(H_(2)O)}_n(2){Tb_(2)(L)_(3)(DMF)_(2)(H_(2)O)]·H_(2)O}n(3) were successfully synthesized from H_(2)L(N,N'-bis(4-carbozylbenzyl)aniline).Remarkably,compound 3 has extremely high sensitivity to *** the concentration of 4-nitrophenol reaches 5.98 × 10^(-3) mmol/L,the fluorescence intensity of compound 3 is quenched by 59%.The calculated K_(SV) is 7.56 × 10~4 and limit of detection(LOD) is 4.73 μg/L.3 exhibits a highly sensitive detection towards Fe^(3+) and compound 2 shows outstanding antibacterial effects on Staphylococcus aureus,re *** white light emission was also investigated by changing the mixing ratio of LaNO_(3),EuNO_(3) and TbNO_(3) during the preparation of coordination polymers.

关键词： Coordination polymers Lanthanide-based Detection Luminescence Antibacterial Rare earths

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Detrital Zircon U-Pb Ages and Geochemistry of the Silurian to Permian Sedimentary Rocks in Central Inner Mongolia, China: Implications for Closure of the Paleo-Asian Ocean

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Acta Geologica Sinica(English Edition) 2019年第5期93卷 1228-1260页

作者： li Yilong WANG Guoqing XIAO Wenjiao ZOU jing ZHENG Jianping Fraukje MBROUWER School of Earth Sciences State Key Laboratory of Geological Processes and Mineral ResourcesChina University of GeosciencesWuhan 430074China State Key Laboratory of lithospheric Evolution Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Geology and Geochemistry group Department of Earth SciencesVU AmsterdamDe Boelelaan 10851081 HV AmsterdamThe Netherlands

The Solonker suture zone has long been considered to mark the location of the final disappearance of the PaleoAsian Ocean in the eastern Central Asian Orogenic Belt(CAOB). However, the time of final suturing is still controversial with two main different proposals of late Permian to early Triassic, and late Devonian. This study reports integrated wholerock geochemistry and LA-ICP-MS zircon U-Pb ages of sedimentary rocks from the Silurian Xuniwusu Formation, the Devonian Xilingol Complex and the Permian Zhesi Formation in the Hegenshan-Xilinhot-linxi area in central Inner Mongolia, China. The depositional environment, provenance and tectonic setting of the Silurian-Devonian and the Permian sediments are compared to constrain the tectonic evolution of the Solonker suture zone and its neighboring zones. The protoliths of the silty slates from the Xuniwusu Formation in the Baolidao zone belong to wacke and were derived from felsic igneous rocks with steady-state weathering, poor sorting and compositional immaturity. The protoliths of metasedimentary rocks from the Xilingol Complex were wackes and litharenites and were sourced from predominantly felsic igneous rocks with variable weathering conditions and moderate sorting. The Xuniwusu Formation and Xilingol Complex samples both have two groups of detrital zircon that peak at ca. 0.9-1.0 Ga and ca. 420-440 Ma, with maximum deposition ages of late Silurian and middle Devonian age, respectively. Considering the ca. 484-383 Ma volcanic arc in the Baolidao zone, the Xuxiniwu Formation represents an oceanic trench sediment and is covered by the sedimentary rocks in the Xilingol Complex that represents a continental slope sediment in front of the arc. The middle Permian Zhesi Formation metasandstones were derived from predominantly felsic igneous rocks and are texturally immature with very low degrees of rounding and sorting, indicating short transport and rapid burial. The Zhesi Formation in the Hegenshan zone has a main zirc

关键词： geochemistry zircon LA-ICP-MS U-Pb dating sedimentary rocks Paleo-Asian Ocean Central Asian Orogenic Belt

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Experimental Realization of Braunstein's Weight-Decision Algorithm

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Chinese Physics Letters 2011年第9期28卷 28-30页

作者： HOU Shi-Yao CUI jing-Xin li Jun-lin State Key Laboratory of Low-dimensional Quantum Physics and Department of Physics Tsinghua UniversityBeijing 100084

Braunstein proposed an algorithm to distinguish the Boolean functions of two different *** we implement the algorithm in a two-qubit nuclear magnetic resonance quantum information *** experiment shows that the algorit... 详细信息

关键词： weights Boolean quantum

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Growth performance,digestive enzyme activities and serum nonspecific immunity of the red tilapia(Oreochromis mossambicus×Oreochromis niloticus)feddiets supplemented with ultrafine powder of Enteromopha prolifera

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Journal of Oceanology and limnology 2018年第5期36卷 1843-1850页

作者： li Zhongbao YANG Huan SHANGGUAN jingbo CHEN Oiang li Wenjing LU jing Fisheries College Jimei UniversitiyXiamen 361021China Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment Xiamen 361021China

The present study evaluated ef fects of ultrafine powder of the green macroalgae E nteromopha prolifera as dietary supplement on growth performance, digestive enzyme activities and serum nonspecific immune responses of the red tilapia(O reochromis mossambicus × Oreochromis niloticus). The red tilapia were fed five diets supplemented with different levels of E. prolifera ultrafine powder as well as a control diet containing no E. prolifera for seven weeks(Diets 0–6 contained 0(control), 10, 20, 30, 40 and 50 g/kg of E. prolifera ultrafine powder, respectively). The results showed that diets supplemented with E. prolifera ultrafine powder generally improved growth, immunity and digestive enzyme activities of the red tilapia. In particular, the fish fed the diet incorporated 50 g/kg (5%) E. prolifera ultrafine powder(Diet 5) achieved the highest percentage weight gain, specific growth rate and the condition factor(increased by 15.4%, 8.0% and 5.7%, respectively when compared to the control). Feeding the diet also led to significantly increases( P line phosphatase, lysozyme activities and serum total protein(increased by 19.4%, 48.1%, 29.5%, 30.3% and 8.7%, respectively) as well as digestive enzyme activities of erepsin, gastric amylase, gastric lipase, pepsin, intestinal amylase and gastric lipase(increased by 15.7%, 33.3%, 16.3%, 21.3%, 52.3% and 28.2%, respectively) than those of the control. Based on these results, it is recommended that the inclusion level of E. prolifera ultrafine powder in the diet of the red tilapia should be 50 g/kg(or 5%).

关键词： Enteromopha prolifera ultrafine powder red tilapia growth performance digestive enzyme activities serum nonspecific immunity

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A supplementary assessment system of AQI-V for comprehensive management and control of air quality in chemical industrial parks

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Journal of Environmental Sciences 2023年第8期130卷 114-125页

作者： Qiaoli Wang Dongping Sheng Chengzhi Wu Deji jing Nana Cheng Xingnong Cai Sujing li jingkai Zhao Wei li Jianmeng Chen College of Environment Zhejiang University of TechnologyHangzhou 310032China Key Laboratory of Biomass Chemical Engineering of the Ministry of Education Institute of Industrial Ecology and EnvironmentCollege of Chemical and Biological EngineeringZhejiang University(Yuquan Campus)Hangzhou 310027China Trinity Consultants Inc.(China office)Hangzhou 310012China Zhejiang Ocean University ZhoushanZhejiang Province 316022China

Volatile organic compounds(VOCs)are the dominant pollutants in industrial ***,they are not generally considered as part of the air quality index(AQI)system,which leads to a biased assessment of pollution in industrial *** this study,a supplementary assessment system of AQI-V was established by analyzing VOCs characteristics with vehicle-mounted PTR-TOFMS instrument,correlation analysis and the standards *** hourly and daily scenarios were considered,and the hierarchical parameter setting was further optimized by field *** hourly and daily assessments revealed the evaluation factors for the discriminability of different air quality levels,practiced value for regional air quality improvement,and the reservation of general dominant ***,the universality testing in ZPIP successfully recognized most of the peaks,with 54.76%,38.39%and 6.85%for O_(3),VOCs and NO_(2) as the dominant pollutant,and reflected the daily ambient air quality condition,togetherwith the dominant *** AQI-V systemwith VOCs sub-index is essential for air quality evaluation in industrial parks,which can further provide scientific support to control the pollution of VOCs and the secondary pollutant,therefore significantly improve the air quality in local industrial parks.

关键词： Air quality index Industrial parks,VOCs sub-index AQI-V system Scenario analysis

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Nodes and Field of Tourist Origins to Ancient Village——A Case Study of Huangcheng Village in Shanxi Province of China

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Chinese Geographical Science 2007年第3期17卷 280-287页

作者： Zhong jing Zhang Jie li Donghe Zhao Yong Lu Song Shi Chunyun Nanjing Univ Dept Land Resources & Tourism Sci Nanjing 210093 Peoples R China Anhui Univ Dept Tourism Management Hefei 230039 Peoples R China Anhui Normal Univ Coll Territorial Resource & Tourism Wuhu 241000 Peoples R China

Based on Hagget's theory of spatial structure,researches on the nodes and field of tourist origins to Huang-cheng Village in Shanxi Province of China have been *** hierarchy structure of tourist origins is analyzed with cluster analysis and the gravity *** field of tourist origins is analyzed with attraction radius index(R)and geography concentration index(G).In the field analysis,R and G of Huangcheng Village are compared with Xidi Village that is a world heritage located in Huangshan City of Anhui Province in *** to comparison of loca-tions of two areas,influential factors for field area of Huangcheng Village are *** is concluded that:1)cluster analysis and gravity model can be complementary methods to each other for nodal hierarchy structure analysis of tour-ist origins;and 2)as far as location is concerned,the weak intensity effect of tourism resources in the tourist region is a major cause for explaining why tourist origins to Huangcheng Village are mainly its neighboring ***,it is suggested that the regional effect of tourist resources should be regarded as a component of destination attractiveness when applying gravity model.

关键词： ancient village tourist origin Huangcheng Village nodal hierarchy structure field

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