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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第11期10卷 847-851页

作者： Yang QIU Xi-xiang li hai-ying ZHI Di SHEN Peng LU Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 China

We examined salt tolerance responsive genes in Pak-choi under salt stress and analyze their potential function. The LRNA differential display was used to screen the transcript derived fragments （TDFs） related to salinity tolerance in tolerant and Loderately tolerant Pak-choi germplasm. Seventy-eight primer combinations generated 101 differential eDNA fragments, which ere divided into 10 expression types. Seven cDNA sequences （GenBank accession Nos. DQ006915-DQ006921） obtained and ,~quenced were highly homologous to some known expression genes or the genes related to the signaling pathways in plants under ifferent abiotic stress.

关键词： Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee Salinity tolerance Gene differential expression cDNA fragments Basic local alignment search tool (BLAST) analysis

Identification of SSR loci in Betula luminifera using birch EST data

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Journal of Forestry Research 2011年第2期22卷 201-204页

作者： LU Yong-quan li hai-ying JIA Qing HUANG Hua-hong TONG Zai-kang The Nurturing Station for the State Key Laboratory of Subtropitical Silviculture Zhejiang Agriculture and Forestry University lin＇an 311300 China

Expressed sequence tags（ESTs） are generated from single-pass sequencing of randomly picked cDNA clones and can be used for development of simple sequence repeat（SSR） markers or ***,EST databases have been developed for only a small number of *** paper provides a case study of the utility of freely available birch EST resources for the development of markers necessary for the genetic analysis of Betula *** on birch EST data,primers for 80 EST-SSR candidate loci were developed and tested in *** these,59 EST-SSR loci yielded single,stable and clear PCR *** then tested the utility of those 59 markers in *** results showed 28（47.6%） yielded stable and clear PCR products for at least one *** *** addition,this study describes a rapid and inexpensive alternative for the development of SSRs in species with scarce available sequence data.

关键词： exploit Betula luminifera birch EST database EST-SSR

Effects of navelbine and docetaxel on gene expression in lung cancer cell strains

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Acta Pharmacologica Sinica 2005年第8期26卷 1017-1024页

作者： li CAI~2, hai-ying DONG, Guang-jie SUI Internal Medicine of Oncology, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, China Internal Medicine of Oncology the Third Affiliated Hospital of Harbin Medical University Harbin China

Aim: To search genes sensitivity to the anti-cancer drugs navelbine (NVB) and docetaxel (DOC) in small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC) cell strains. Methods: The sensitivity of 4 strains of SCLC and 6 strains of NSCLC to NVB and DOC was evaluated using the MTT assay. The expression of 1291 sensitive-related genes to the anti-cancer drugs in 10 lung cancer cell strains was measured using cDNA macroarrays and the relationship was analyzed. Results: In total, there were 56(r≥0.4) genes sensitive to NVB and DOC. For NVB: 36 genes were sensitive to NVB, 20 co-expressed genes between the SCLC+NSCLC set and the NSCLC set; 27 expressed genes and 7 specially expressed genes in the SCLC+NSCLC set; and 29 expressed genes and 9 specially expressed genes in the NSCLC set. For DOC, 50 genes were sensitive to DOC, 12 co-expressed genes between the SCLC+NSCLC set and the NSCLC set; 24 expressed genes and 12 specially expressed genes in the SCLC+NSCLC set; and 38 expressed genes and 26 specially xpressed genes in the NSCLC set. The genes sensitive to NVB and DOC in lung-cancer cell stains were mainly divided into the following 4 categories: signal transduction molecules, cell factors, transcription factors and metabolism-related enzymes and inhibitors. Conclusions: There were obvious differences in genes related to NVB and DOC between SCLC and NSCLC cell strains, but the same as categories of function.

关键词： cDNA macroarray lung neoplasms navelbine docetaxel

A comparison on DNA methylation levels in bamboo at five developmental stages

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Journal of Forestry Research 2012年第1期23卷 157-159页

作者： LU Yong-quan WANG Dong-mei li hai-ying JIA Qing WU Ze LU Wen-feng The Nurturing Station for the State Key Laboratory of Subtropical Sil-viculture Zhejiang Agriculture and Forestry UniversityLin'an 311300P.R.China

Bamboo shoots grow quickly through the rapid elongation of intemodes. In order to veri~ whether DNA methylation affects bamboo shoot elongation, we compared DNA methylation levels at five develop- mental stages of bamboo （Phyllostachys praecox）, including young bamboo shoot of 40-cm at length （S1）, bamboo shoot of 2-cm at length （S2）, young leaf （Y1）, leaf （Y2）, and aging leaf （M）, by using amplified fragment length polymorphism （AFLP） based on KpnI/MseI and Acc65I/MseI platforms. The polymorphism ratio of methylated to un- methylated DNA sites in bamboo shoot （$1 and $2） was higher than that in leaf （Y1, Y2 and M）. The polymorphism ratio of methylated to un- methylated DNA sites in $2 was highest （32%）. Our results suggest that DNA methylation changed greatly at bamboo growing stages, especially in stage of rapid elongation of intemodes.

关键词： mcthylation bamboo shoot Phyllostachys praecox inter-node elongation

Several Properties of p-w-hyponormal Operators

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Chinese Quarterly Journal of Mathematics 2008年第2期23卷 195-201页

作者： li hai-ying YANG Chang-sen Department of Mathematics Henan Normal University Xinxiang 453007 China School of Mathematics and Statistics Wuhan University Wuhan 430072 China

In this paper, let T be a bounded linear operator on a complex Hilbert H. We give and prove that every p-w-hyponormal operator has Bishop's property(β) and spectral properties; Quasi-similar p-w-hyponormal operators have equal spectra and equal essential spectra. Finally, for p-w-hyponormal operators, we give a kind of proof of its normality by use of properties of partial isometry.

关键词： Aluthge transformation Bishop's property(β) quasi-similar w-hyponormal operators p-w-hyponormal operators

In Silico Screening of Potential Spike Glycoprotein Inhibitors of SARS-CoV-2 with Drug Repurposing Strategy

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Chinese Journal of Integrative Medicine 2020年第9期26卷 663-669页

作者： WEI Tian-zi WANG Hao WU Xue-qing LU Yi GUAN Sheng-hui DONG Feng-quan DONG Chen-le ZHU Gu-li BAO Yu-zhou ZHANG Jian WANG Guan-yu li hai-ying Department of Biology Southern University of Science and TechnologyShenzhen(518055)GuangdongChina School of Medicine Southern University of Science and TechnologyShenzhen(518055)GuangdongChina School of Biomedical Sciences Li Ka Shing Faculty of Medicinethe University of Hong KongHong Kong SARChina Department of Obstetrics and Gynecology Shenzhen University General HospitalShenzhen(518055)GuangdongChina Clinical Medical Academy Shenzhen UniversityShenzhen(518055)GuangdongChina Department of Cardiology Shenzhen University General HospitalShenzhen(518055)GuangdongChina Department of Obstetrics and Gynecology the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou(325000)ZhejiangChina Guangdong Provincial Key Laboratory of Computational Science and Material Design Shenzhen(518055)GuangdongChina Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research Shenzhen(518055)GuangdongChina

Objective:To select potential molecules that can target viral spike proteins,which may potentially interrupt the interaction between the human angiotension-converting enzyme 2(ACE2)receptor and viral spike protein by virtual ***:The three-dimensional(3D)-coordinate file of the receptor-binding domain(RBD)-ACE2 complex for searching a suitable docking pocket was firstly downloaded and ***,approximately 15,000 molecular candidates were prepared,including US Food and Drug Administration(FDA)-approved drngs from DrugBank and natural compounds from Traditional Chinese Medicine Systems Pharmacology(TCMSP),for the docking ***,virtual screening was performed and the binding energy in Autodock Vina was ***,the top 20 molecules with high binding energy and their Chinese medicine(CM)herb sources were listed in this ***:It was found that digitoxin,a cardiac glycoside in DrugBank and bisindigotin in TCMSP had the highest docking ***,two of the CM herbs containing the natural compounds that had relatively high binding scores,Forsyfh/ae frucft/s and/saf/d/s racWx,are components of lianhua Qingwen(莲花清痕),a CM formula reportedly exerting activity against severe acute respiratory syndrome(SARS)-***,raltegravir,an HIV integrase inhibitor,was found to have a relatively high binding ***:A class of compounds,which are from FDA-approved drugs and CM natural compounds,that had high binding energy with RBD of the viral spike *** work provides potential candidates for other researchers to identify inhibitors to prevent SARS-CoV-2 infection,and highlights the importance of CM and integrative application of CM and Western medicine on treating COVID-19.

关键词： COVID-19 SARS-CoV-2 drug repurposing virtual screening Chinese medicine

Improved social force model based on exit selection for microscopic pedestrian simulation in subway station

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Journal of Central South University 2015年第11期22卷 4490-4497页

作者：郑勋李海鹰孟令云许心越陈旭 State Key Laboratory of Rail Traffic Control and Safety (Beijing Jiaotong University) School of Traffic and Transportation Beijing Jiaotong University

An improved social force model based on exit selection is proposed to simulate pedestrians' microscopic behaviors in subway station. The modification lies in considering three factors of spatial distance, occupant density and exit width. In addition, the problem of pedestrians selecting exit frequently is solved as follows: not changing to other exits in the affected area of one exit, using the probability of remaining preceding exit and invoking function of exit selection after several simulation steps. Pedestrians in subway station have some special characteristics, such as explicit destinations, different familiarities with subway station. Finally, Beijing Zoo Subway Station is taken as an example and the feasibility of the model results is verified through the comparison of the actual data and simulation data. The simulation results show that the improved model can depict the microscopic behaviors of pedestrians in subway station.

关键词： exit selection social force model exit width micro

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二维高斯波束对多层球粒子电磁散射的解析解

物理学报 2008年第2期57卷 833-838页

作者：李海英吴振森西安电子科技大学理学院西安710071

基于矢量波函数在球和柱坐标系中表达式之间的转换关系,提出了一种求解球坐标系中二维高斯波束波形因子的方法,得到了二维高斯波束波形因子在球坐标系中的解析公式.结合广义米理论推导了在轴二维高斯波束入射多层球粒子的电磁散射的解析... 详细信息

基于矢量波函数在球和柱坐标系中表达式之间的转换关系,提出了一种求解球坐标系中二维高斯波束波形因子的方法,得到了二维高斯波束波形因子在球坐标系中的解析公式.结合广义米理论推导了在轴二维高斯波束入射多层球粒子的电磁散射的解析解,并对散射强度随散射角的分布进行了数值模拟,结果与平面波入射情况进行了比较.

关键词：矢量波函数波形因子电磁散射广义米理论

Oxygen vacancies enriched Ir/WOx catalysts for the directly chem-catalytic conversion of cellulose to ethanol

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Tungsten 2025年第1期7卷 88-99页

作者： Yu-Jing Weng Zhao-ying Ding ying-Chao li Yuan-Feng Wu Yuan-Yang Xu Rui Chen Xiao-Lei Zhao hai-Yong Wang Da-Lei Zhang Yu-Long Zhang Henan Key Laboratory of Coal Green Conversion and International Joint Laboratory of Coal Clean Utilization College of Chemistry and Chemical Engineering Henan Polytechnic University Key Laboratory of Renewable Energy Guangzhou Institute of Energy Conversion Chinese Academy of Sciences liaoning Institute of Energy Technology

Tungsten and its oxides are highly versatile catalysts with unique properties that hold great promise for a wide array of applications,including biological pathways and chemical *** chemo-catalysis of cellulose to ethanol presents a promising avenue for ethanol synthesis with the potential for enhanced efficiency compared to conventional methods,offering a sustainable and carbon-neutral alternative to existing petrochemical and biological ***,Ir/WOxcatalysts enriched with oxygen vacancies were meticulously prepared and demonstrated remarkable catalytic performance in converting cellulose to ethanol directly in the presence of tungstic *** catalyst achieved a high ethanol yield of 66.5%under optimal reaction conditions,approaching the theoretical yield limit of traditional ethanol *** results revealed the presence of oxygen defects,Bronsted/Lewis acidity,and Ir nanoclusters on the catalyst surface,working synergistically to facilitate the cascade catalysis of cellulose hydrolysis,sugar retro-aldol condensation reaction,and C2 intermediate hydrodeoxygenation to *** situ Fourier transform infrared spectrometer analysis confirmed the catalyst's ability to enhance the adsorption and activation of ethylene glycol for ethanol *** manipulating subtle structural features,this study offers a fresh perspective on tungsten group catalyst design and underscores the importance of synergistic effects among catalytic sites for the efficient production of ethanol from cellulose.

关键词： Cellulosic ethanol Chem-catalysis Hydrogenolysis Retro-aldol condensation Tungsten

On p-ω-hyponormal Operators

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Chinese Quarterly Journal of Mathematics 2005年第1期20卷 79-84页

作者： YANGChang-sen lihai-ying GollegeofMathematicsandInformationScience HenanNormalUniversityXinxiang453002China

In this paper, we show that if T is p-ω-hyponormal, the nonzero points of the approximate and joint approximate point spectrum of T are identical; Moreover, we obtain a pair of inequalities similar to p-ω-hyponormal... 详细信息

关键词： Furuta inequality Lowner-Heinz inequality approximate point spectrum p-ω-hyponormal operators