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Therapeutic effects of adipose-derived stem cells-based microtissues on erectile dysfunction in streptozotocin-induced diabetic rats

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Asian Journal of Andrology 2017年第1期19卷 91-97页

作者： feng Zhou hua Xin Yong-De Xu Hong-En Lei Bi-Cheng Yang Rui-li Guan Meng li Jian-Quan Hou Zhong-Cheng Xin Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing 100034 China department of Urology First Affiliated Hospital of Soochow University Soochow University Suzhou 215006 China department of Ophthalmology Beijing Chao-Yang Hospital Capital Medical University Beijing 100043 China department of Urology General Hospital of Ningxia Medical University Ningxia Medical University Ningxia 750021 China

This study aimed to explore the therapeutic effects of adipose-derived stem cells （ADSCs）-based microtissues （MTs） on erectile dysfunction （ED） in streptozotocin （STZ）-induced diabetic rats. Fifty-six 8-week-old Sprague-Dawley rats received intraperitoneal injection of STZ （60 mg kg-1）, and 8 weeks later, the determined diabetic rats randomly received intracavernous （IC） injection of phosphate buffer solution （PBS）, ADSCs, or MTs. Another eight normal rats equally got IC injection of PBS. MTs were generated with a hanging drop method, and the injected cells were tracked in ADSC- and MT-injected rats. Four weeks after the treatments, intracavernous pressure （ICP）, histopathological changes in corpus cavernosum （CC）, and functional proteins were measured. Rat cytokine antibody array was used to detect ADSCs or MTs lysate. The results showed that MTs expressed vascular endothelial growth factor （VEGF）, nerve growth factor （NGF）, and tumor necrosis factor-stimulated gene-6 （TSG-6）. MTs injection had a higher retention than ADSCs injection and MTs treatment improved ICP, neuronal nitric oxide synthase （nNOS） expression, smooth muscle, and endothelial contents in diabetic rats, ameliorated local inflammation in CC better. Thus, our findings demonstrate that IC injection of MTs improves erectile function and histopathological changes in STZ-induced diabetic rats and appears to be more promising than traditional ADSCs. The underlying mechanisms involve increased cell retention accompanied with neuroprotection and anti-inflammatory behaviors of the paracrine factors.

关键词： adipose-derived stem cells diabetes erectile dysfunction microtissues paracrine factors

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大肠癌COX-2表达和p53突变

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复旦学报（医学版） 2003年第2期30卷 137-138,142,F002页

作者：黄若凡林庚金唐峰徐三荣李华复旦大学附属华山医院消化科上海200040 复旦大学附属华山医院病理科上海200040 上海医学院病理学系上海200032

目的　探讨大肠癌COX 2表达和 p5 3突变的关系。方法　手术切除的大肠癌标本 4 9例 ,正常大肠组织标本 10例。免疫组化检测大肠癌及正常大肠组织COX 2表达水平及突变型 p5 3表达。结果　突变型 p5 3表达阳性组和阴性组COX 2表达有显... 详细信息

目的　探讨大肠癌COX 2表达和 p5 3突变的关系。方法　手术切除的大肠癌标本 4 9例 ,正常大肠组织标本 10例。免疫组化检测大肠癌及正常大肠组织COX 2表达水平及突变型 p5 3表达。结果　突变型 p5 3表达阳性组和阴性组COX 2表达有显著差异 (P <0 .0 5 )。结论　大肠癌COX 2表达可能和p5

关键词：大肠癌 COX-2 p53 基因表达基因突变免疫组织化学

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北京山区资源信息管理系统的研建与应用

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首都师范大学学报（自然科学版） 1997年第2期18卷 113-118页

作者：奚为民傅桦任亚楠黎剑首都师范大学地理环境与发展研究所首都师范大学计算机系多媒体技术应用研究所

研究了以奔腾系列微机和Mapinfo软件系统为主要手段构建北京山区资源管理空间信息系统的过程,包括系统的结构化设计、实施、研建等,最后分析了本系统在资源管理、分析评价、资源开发规划等领域的应用。

关键词：北京山区资源管理管理系统资源开发 Mapinfo软件规划分析评价资源信息奔腾微机

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CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai Haiying Cai huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin Chen Danping Chen Fuqing Chen Fusan Chen Gang Chen Guoming Chen hua-Xing Chen Huirun Chen Jinhui Chen Ji-Yuan Chen Kai Chen Mali Chen Mingjun Chen Mingshui Chen Ning Chen Shanhong Chen Shanzhen Chen Shao-Long Chen Shaomin Chen Shiqiang Chen Tianlu Chen Wei Chen Xiang Chen Xiaoyu Chen Xin Chen Xun Chen Xurong Chen Ye Chen Ying Chen Yukai Chen Zelin Chen Zilin Chen Gang Chen Boping Chen Chunhui Chen Hok Chuen Cheng huajie Cheng Shan Cheng Tongguang Cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding Haiyi Dong Jianin AGH university of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui university HefeiAnhui Applied Physics department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu university Institute of ScienceVaranasi Bandirma Onyedi Eylul university Bandirma Beihang university Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing normal university Beijing Brown university ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad university Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo university Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central china normal university WuhanHubei Central South university ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa Chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan china academy of engineering physics MianyangSichuan china building materials academy Beijing china Center of Advanced Science and Technology Beijing china Institute of Atomic Energy Beijing china Jiliang university HangzhouZhejiang china Nuclear Instrument CO. LTDBeijing china university of Geosciences Beijing china university of Geosciences WuhanHubei china university of Mining and Technology XuzhouJiangsu china West normal university NanchongSichuan Chongqing university Department of PhysicsChongqing Chulalongkorn university Bangkok City university of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell university IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu university DalianLiaoning Dalian university of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East china university Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by china,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

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Construction and characterization of a full-lengh cDNA library from non-fresh Giardia lamblia

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Asian Pacific Journal of Tropical Medicine 2012年第12期5卷 931-934页

作者： Jun-li Guo Jie Jiang Wen-Yu Zheng Ming-Luan li Xi-feng Tian Xian-Min feng Yue-hua Wang Xiao-Hong Ju Yue-Qiong Kong School of Laboratory Medicine Jilin Medical College Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical College department of Hand Microsurgery Central Hospital of Jilin City College of life Science Hebei united university

Objective:To construct rapidly a full-length cDNA library from nanogram amounts total RIMA of Giardia lamblia(***) trophozoites stocked in RNA stabilization ***:Total RNA of Giardia was extracted using Trizol reagent.A full-length cDNA library of *** trophozoites was constructed by a long-distance PCR(LD-PCR) *** recombinant rate and the coverage rate of full-length clones of the library were *** inserted fragments were identified and sequenced by PCR ***:The titer of cDNA library was 3.85×10~7 pfu/*** length of inserted fragments ranged from 0.4 to 2.5 kb,and the recombination efficiency accounted for 100%(20/20).The coverage rate of full-length clones is high(17/20). Conclusions:The RNA stabilization reagent may be used to fix the cells and prevent the RNA in cells even though delivered under normal atmospheric *** long-distance PCR can be used to construct a full-length cDNA library rapidly and it needs less RNA than the traditional method from mRNA.

关键词： Giardia lamblia cDNA library RNA stabilization reagent

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Application of Computer-Aided Tongue Inspection for Preliminary Screening of Esophageal Cancer

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Chinese Journal of Integrative Medicine 2018年第10期24卷 746-751页

作者： DUAN Jin-long DENG Bo SONG Guo-hui CHEN Zhi-feng GONG Yan-wei HE Yu-hua JIA li-qun School of Clinical Medicine Beijing University of Chinese Medicine department of Oncology of Integrative Chinese and Western Medicine China-Japan Friendship Hospital department of Oncology Ci County Tumor Hospital department of Oncology Fourth Affiliated Hospital of Hebei Medical University

Objective： To differentiate patients with esophageal cancer or premalignant lesions from the high-risk population for preliminary screening of esophageal cancer using a feature index determined by a computer-aided tongue information acquisition and processing system （DS01-B）. Methods： Totally, 213 patients diagnosed with esophageal cancer or premalignant lesions and 2,840 normal subjects were collected including primarily screened and reexamined, all of them were confirmed with histological examinations. Their tongue color space values and manifestation features were extracted by DS01-B and analyzed. Firstly, the analysis of variance was performed to differentiate normal subjects from patients with esophageal cancer and premalignant lesions. Secondly, the logistic regression was conducted using 10 features and gender, age to get a predictive equation of the possibility of esophageal cancer or premalignant lesions. Lastly, the equation was tested by subjects undergoing primary screening. Results： Saturation （S） values in the HSV color space showed significant differences between patients with esophageal cancer and normal subjects or those with mild atypical hyperplasia （P〈0.05）; blue-to-yellow （b） values in the Lab color space showed significant differences between patients with esophageal cancer or premalignant lesions and normal subjects （P〈0.05）. Logistic regression analysis showed that the computer-aided tongue inspection approach had an accuracy of 72.3% （2008/2776） in identifying patients with esophageal cancer or premalignant lesions for preliminary screening in high-risk population. Conclusion： Computer-aided tongue inspection, with descriptive and quantitative profile as described in this study, could be applied as a cost- and time- efficient, non-invasive approach for preliminary screening of esophageal cancer in high-risk population.

关键词： esophageal cancer preliminary differential diagnosis computer-aided tongue inspection logistic regression

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Optimal immunosuppressor induces stable gut microbiota after liver transplantation

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World Journal of Gastroenterology 2018年第34期24卷 3871-3883页

作者： Jian-Wen Jiang Zhi-Gang Ren Hai-feng Lu hua Zhang Ang li Guang-Ying Cui Jun-Jun Jia Hai-Yang Xie Xin-hua Chen Yong He li Jiang Lan-Juan li department of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital School of Medicine Zhejiang University Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang University Health Management Center First Affiliated Hospital School of Medicine Zhejiang University department of Infectious Diseases Precision Medicine Center the First Affiliated Hospital of Zhengzhou University State Key Laboratory for Diagnosis and Treatment of Infectious Disease First Affiliated Hospital School of Medicine Zhejiang University

AIM To study the influence of different doses of tacrolimus(FK506)on gut microbiota after liver transplantation(LT)in *** Specific pathogen-free Brown Norway(BN)rats and Lewis rats were separated into five groups:(1)Tolerance group(BN-BN LT,n=8);(2)rejection group(Lewis-BN LT,n=8);(3)high dosage FK506(FK506-H)group(Lewis-BN LT,n=8);(4)middle dosage FK506(FK506-M)group(Lewis-BN LT,n=8);and(5)low dosage FK506(FK506-L)group(LewisBN LT,n=8).FK506 was administered to recipients at a dose of 1.0 mg/kg,0.5 mg/kg,and 0.1 mg/kg body weight for 29 d after LT to the FK506-H,FK506-M,and FK506-L groups,*** the 30^(th) day after LT,all rats were sampled and *** samples were harvested for liver function and plasma endotoxin *** graft and ileocecal tissues were collected for histopathology *** contents were used for DNA extraction,Real-time quantitative polymerase chain reaction(RT-PCR)and digital processing of denaturing gradient gel electrophoresis(DGGE)profiles and *** Compared to the FK506-H and FK506-L groups,FK506-M was optimal for maintaining immunosuppression and inducing normal graft function;the FK506-M maintained gut barrier integrity and low plasma endotoxin levels;furthermore,DGGE results showed that FK506-M induced stable gut *** analysis indicated that FK506-M increased species richness and rare species abundance,and cluster analysis confirmed the stable gut microbiota induced by *** tree analysis identified crucial bacteria associated with FK506-M;seven of the nine bacteria that were decreased corresponded to Bacteroidetes,while increased bacteria were of the Bifidobacterium ***506-M increased Faecalibacterium prausnitzii and Bifidobacterium *** decreased Bacteroides-Prevotella and Enterobacteriaceae,as assessed by RT-PCR,which confirmed the crucial bacterial alterations identified through *** Compared to the low or high dosage of FK5

关键词： liver transplantation Graft function Gut microbiota Immunosuppressor Tacrolimus Rejection Denaturing gradient gel electrophoresis

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Synthesis and Anticancer Activity of a New Di-nuclear Ruthenium Arene Compound with Thiosemicarbazones

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Chinese Journal of Structural Chemistry 2020年第6期39卷 1112-1118页

作者： SU Wei ZHANG Bao-Qu PENG Bing-hua TANG Zhao-feng li Pei-Yuan Key Laboratory of Guangxi Key Laboratory of Natural Polymer Chemistry and Physics(Nanning normal university) Nanning 530001China College of Pharmacy Guangxi University of Chinese MedicineNanning 530200China

A new di-nuclear ruthenium arene compound containing thiosemicarbazone ligands, [Ru(η6-cymene)(TSC)Cl]2(PF6)2(2), was synthesized and characterized by 1H NMR, HR-ESI-MS, and elemental analysis. Furthermore, the complete structure of 2 was determined by single-crystal X-ray diffraction analysis. Density functional theory(DFT) calculations with B3LYP functional and LANL2DZ(for Ru)/6-31G*(for other atoms) basis sets were carried out for the reactions involving complexes 1 and 2 in order to understand the formation of the complexes. The in vitro anticancer activities of the compound were investigated against five human cancer cell lines(SGC-7901, CNE-2, HepG2, KB and HeLa), and the IC50 values are 39.5, 45.8, 49.8, 54.5 and 61.8 μM, respectively.

关键词： ruthenium compound DFT anticancer

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北京市房山区经济区位优势研究 Ⅱ.山区经济的区域差异

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首都师范大学学报（自然科学版） 1998年第1期19卷 81-87页

作者：傅桦吴雁华毕维铭李廷芳首都师范大学地理系北京市第三师范学校中师部

研究影响山区经济差异的主要因素，根据调查数据和资料，对３个经济区域的优劣势进行分析，提出了相应的发展方向．

关键词：房山区山区经济差异

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CT联合DCE-MRI在鉴别骶尾部骨巨细胞瘤和脊索瘤的价值研究

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中国CT和MRI杂志 2024年第5期22卷 152-154页

作者：袁逍李玮马冯慧李燕燕赵海艳鲍花左林空军军医大学附属第二医院放射科陕西西安710038 空军军医大学附属第二医院康复理疗科陕西西安710038

目的CT联合DCE-MRI-TIC曲线变化分析对比骶尾部脊索瘤和骨巨细胞瘤影像特征。方法回顾性分析35例骶尾部脊索瘤及骨巨细胞瘤患者的CT、核磁共振影像资料,所有病例均有手术病理结果。脊索瘤患者21人,骨巨细胞瘤患者14人。病程15天至5年。... 详细信息

目的CT联合DCE-MRI-TIC曲线变化分析对比骶尾部脊索瘤和骨巨细胞瘤影像特征。方法回顾性分析35例骶尾部脊索瘤及骨巨细胞瘤患者的CT、核磁共振影像资料,所有病例均有手术病理结果。脊索瘤患者21人,骨巨细胞瘤患者14人。病程15天至5年。结果35名患者全部接受了CT平扫、DCEMRI检查。19例脊索瘤患者肿瘤多发生于S3椎体以下部位,靠近脊椎中线;12例骨巨细胞瘤以S2椎体及以上部位发生、偏离中轴骨,8例骨巨细胞瘤累及骶髂关节。脊索瘤CT影像上常表现为形态不规则,可见残存骨或钙质密度影,以致正常椎体形态消失或被包块代替;而骨巨细胞瘤常造成椎体骨壳不完整,但椎体轮廓尚可见。MRI上脊索瘤病灶内见高或更高T2信号影伴点、片状低信号结节,骨巨细胞瘤多呈等或稍高信号影。DCE-MRI脊索瘤具有延迟强的特点,TIC曲线类型多呈流入型;而大部分骨巨细胞瘤多早期呈显著强化而后造影剂迅速减退,信号减低;MCER、EP平均值较脊索瘤组明显升高,TIC曲线类型多呈流出型。结论CT联合DEC-MRI有助于骶尾部脊索瘤与骨巨细胞瘤的诊断及鉴别诊断,能为临床提供可靠的影像依据。

关键词：脊索瘤骨巨细胞瘤 CT DCE-MRI TIC曲线鉴别诊断

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