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Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosomes deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1

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Science China(life Sciences) 2005年第6期48卷 574-584页

作者： li feng1,{1.,{2., wang zhenfang1 & li jilun1 1. state key laboratory for agro-biotechnology and department of microbiology, China agricultural university, beijing 100094, China 2. department of Biology, Huaibei Coal Industry Teachers’ College, Huaibei 2.5000, China 1. state {1. {2. for {2.-biotechnology and department of microbiology China Agricultural University 100094 Beijing China 2. {1. of Biology Huaibei Coal Industry Teachers’ College 235000 Huaibei China

A magnetosome deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1.was generated by mini-Tn5 transposon mutagenesis, and a 5045-bp fragment flanking mini-Tn5 in NM4 was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved six putative open reading frames (ORFs); the mini-Tn5 was inserted into ORF4. Functional complementary test indicated that the 5045-bp fragment was required for biosynthesis of mag-netosomes in M. gryphiswaldense MSR-1. The protein encoded by ORF4 had 2.% of identity with the chemotaxis protein CheYIII of Caulobacter crescentus CB1., and the protein encoded by ORF4 contained a conserved signal receiver domain that can receive the signal from the sensor partner of the bacterial two-component systems. It was suggested that the protein en-coded by ORF4 may take part in the signal transduction relating to biosynthesis of magneto-somes.

关键词： Magnetospirillum gryphiswaldense, magnetosome deleted mutant, gene cloning, functional analysis.

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Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosome-deleted mutant NM2. of Magnetospirillum gryphiswaldense MSR-1

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Chinese Science Bulletin 2009年第9期54卷 1522-1528页

作者： li {1. li ying {2. wei wang zhenfang li jilun state {1. {2. for {2.-biotechnology and department of microbiology China Agricultural University Beijing 100193 China School of life Science Anhui Key Laboratory of Plant Resources and Biology Huaibei Coal Industry Teachers' College Huaibei 235000 China

A magnetosome-deleted mutant NM2. of Magnetospirillum gryphiswaldense MSR-1.was generated by mini-Tn5 lacZ2.transposon mutagenesis, and a 3073-bp fragment flanking mini-Tn5 lacZ2.in NM2. was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved three putative ORFs; the mini-Tn5 lacZ2.was inserted into ORF1. Functional complementary test indicated that the 3073-bp fragment was required for biosynthesis of magnetosomes in M. gryphiswaldense MSR-1. The majority of proteins, which had homology with the protein encoded by ORF1. were the cation transporter. Transmembrane domain analysis showed that the protein encoded by ORF1.contained four trans-membrane domains. It may be a transmembrane protein. The protein encoded by ORF1.contained two putative conserved domains: COG0053 and PRK09509. The MMT1.and FieF, containing conserved do-mains COG0053 and PRK09509 too, were Fe2. transporter (cation diffusion facilitator superfamily). It was suggested that the protein encoded by ORF1.might take part in the magnetosomes biosynthesis as Fe2. transporter.

关键词： Tn5诱变科学研究缺失突变序列分析磁小体海洋迷你克隆

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CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumorassociated macrophage polarization via FBXW7-N0TCHCCL2. CSF1.axis

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Signal Transduction and Targeted Therapy 2021年第2期6卷 482-495页

作者： Yao-Hui Huang Kun Cai Peng-Peng Xu li {1. Chuan-Xin Huang {2. Fang Shu Cheng Xiao Jian Sun {2. liu Jin-Yan Huang Meng-Meng Ji wei-li Zhao Shanghai Istitute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine at ShanghaiRulin Hospital Afliated to Shanghai Jiao Tong University School of MedicineShanghaiChina School of life Sciences and {1..Shanghai Jiao Tong Universiy ShanghalChina department of Immunobiology and {1. Shanghai Istitute of ImmunologyShanghai Jiao Tong University School of MedicineShanghaiChina Pole de Recherches Sino Francais en Science du Vivant et Genomique Laboratory of Molecular PathologyShanghalChina

Epigenetic alterations play an important role in tumor progression of diffuse large B-cell lymphoma(DL BCL).However,the biological relevance of epigenetic gene mutations on tumor microenvironment remains to be *** core set of genes relating to histone methylation(KMT2.,KMT2.,EZH2.,histone acetylation(CREBBP,EP300),DNA methylation(TET2.,and chromatin remodeling(ARID1.)were detected in the training cohort of 31. patients by whole-genome/exome sequencing(WGS/WES)and in the validation cohort of 303 patients with newly diagnosed DL BCL by targeted *** correlation with peripheral blood immune cells and clinical outcomes were *** mechanisms on tumor microenvironment were investigated both in vitro and in *** all 61. DLBCL patients,somatic mutations in KMT2.(1..5%)were most frequently observed,followed by mutations in ARID1.(8.7%),CREBBP(8.4%),KMT2.(8.2.),TET2.7.8%),EP300(6.8%),and EZH2.2.9%).Among them,CREBBP/EP300 mutations were signifcantly associated with decreased peripheral blood absolute lymphocyte-to-monocyte ratios,as well as inferior progression-free and overall *** B-lymphoma cells,the mutation or knockdown of CREBBP or EP300 inhibited H3K2. acetylation,downregulated FBXW7 expression,activated the NOTCH pathway,and downstream CCL2.CSF1.expression,resulting in tumor-associated macrophage polarization to M2.phenotype and tumor cell *** B-lymphoma murine models,xenografted tumors bearing CREBBP/EP300 mutation presented lower H3K2. acetylation,higher M2.macrophage recruitment,and more rapid tumor growth than those with CREBBP/EP300 wild-type control via FBXW7-NOTCH-CCL2.CSF1.*** work thus contributed to the understanding of aberrant histone acetylation regulation on tumor microenvironment as an alternative mechanism of tumor progression in DLBCL.

关键词： FBXW7 P300 macrophage

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Immunosuppressive therapy selectively modulates B-cell responses in patients with rheumatic and musculoskeletal diseases receiving the inactivated COVID-1. vaccine

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Rheumatology & Autoimmunity 2023年第2期3卷 78-89页

作者： Rui li Ruru Guo Jia li Bing Zheng Ronghao Xu Xiaoyue {2. yingying Chen Guo Tang Qianqian li Yixuan li Jiaqi feng Xiaoxiang Chen ying wang Shuang Ye Fang Du liangjing Lu Jun Deng department of Rheumatology Renji HospitalShanghai Jiao Tong University School of MedicineShanghaiChina department of {1. Medicine Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina department of Immunology and {1. State Key Laboratory of Oncogenes and Related GenesShanghai Institute of ImmunologyShanghai Jiao Tong University School of MedicineShanghaiChina state {1. {2. of Oncogenes and Related Genes Shanghai Cancer InstituteRenji HospitalShanghai Jiao Tong University School of MedicineShanghaiChina

Background:Immunosuppressive medication reduces the immunogenicity of the coronavirus disease 2.1.(COVID-1.)vaccines in patients with rheumatic and musculoskeletal diseases(RMDs).However,the underl{1. mechanism remains *** primary aim of our study was to dissect the impact of immunosuppressive medication on cellular and humoral immune responses in RMD patients receiving the inactivated COVID-1. ***:A total of 2. RMD patients and five healthy controls(HCs)receiving two doses of the inactivated COVID-1. vaccine(Sinovac-CoronaVac)were prospectively *** samples were collected before the primary vaccination(Week 0)and one week after the second vaccination(Week 5).Neutralizing antibody(nAb)titers and autoantibody titers were measured by a pseudovirus-based neutralization assay and enzyme-linked immunosorbent assay,***4^(+)T-cell and CD1.^(+)B-cell subsets and serum cytokines were analyzed by flow ***:The inactivated COVID-1. vaccine was immunogenic in RMD patients and HCs after the second vaccination,but the nAb titers were lower in RMD patients than those in *** patients with systemic lupus erythematosus(SLE)had notably increased nAb ***,IgG^(+)CD2.^(+),IgG^(+)IgG1.(+),and IgG^(+)IgG1.(−)B cells were reduced,whereas IgG−IgG1.(+)B cells,and total IgA and IgG titers were markedly ***,Tfh cell and Tfr cell subsets and cytokines produced by Tfh cells were not *** flare rate was low in RMD patients with comparable autoantibody titers,unchanged CD4^(+)T cell subsets and serum proinflammatory cytokines(interleukin[IL]-6,IL-1.,interferon-γ,and tumor necrosis factor-α)after the second ***:Immunosuppressive therapy decreased the immunogenicity of the vaccine and maintained a low flare rate by selectively modulating B cell but not CD4^(+)T cell responses in RMD patients receiving the inactivated COVID-1. *** of the treatment regimen might ensure

关键词： COVID-1. vaccination humoral and cellular responses immunosuppressive medication rheumatic and musculoskeletal diseases

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Biomarkers of aging

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Science China(life Sciences) 2023年第5期66卷 893-1066页

作者： Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri yingjie Ding Jun{2.n feng Jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping {2. ying Jing Dingfeng {2. Jiaming {2. Jingyi {2. Qinhao {2.ang Rui {2.ang feng {2.u Xiaoqian {2.u Zuojun {2.u Oscar Junhong Luo Jianwei Lv Jingyi Ma Kehang Mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang wang Qiaoran wang Siyuan wang Xuan wang Yaning wang Yuhan wang Rimo Wu Kai Xia Fu-Hui Xiao {2.ngyan Xu yingying Xu Haoteng Yan {2.ang Yang Ruici Yang Yuanxin Yang Yi{2.n ying Le Zhang weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou Rui Zhou Qingchen Zhu Zhengmao Zhu feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen weimin Ci Bi-Sen Ding Qiurong Ding feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng Kong Ji {2. Jian {2. Xin {2. Baohua {2.u feng {2.u {2.n {2.u Qiang {2.u Qiang {2.u Xingguo {2.u Yong {2.u Xianghang Luo Shuai Ma Xinran Ma Zhiyong Mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang Yi Eve Sun Yu Sun Mei Tian Shusen wang Si wang Xia wang Xiaoning wang Yan-{2. wang Yunfang wang Catherine CL Wong Andy Peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui Yue Cuntai Zhang Hongbo Zhang {2.ang Zhang weiqi Zhang Yong Zhang Yun-Wu Zhang Zhuohua Zhang Tongbiao Zhao Yuzheng Zhao Dahai Zhu weiguo Zou Gang Pei Guang-Hui {2.u Aging Biomarker Consortium(ABC) Beijing 100101China CAS key {1. of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China state {1. {2. of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China department of Dermatology Xiangya HospitalCentral South UniversityChangsha 410008China Hunan key {1. of Aging Biology Xiangya HospitalCentral South UniversityChangsha 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Clinic Center of Human Gene Research Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Clinical Research Center of Metabolic and Cardiovascular Disease Huazhong University of Science and TechnologyWuhan 430022China Hubei key {1. of Metabo{2.c Abnorma{2.ties and Vascular Aging Huazhong University of Science and TechnologyWuhan 430022China Stem Cell Translational Research Center Tongji HospitalTongji University School of MedicineShanghai 200065China department of Nuclear Medicine Daping HospitalThird Military Medical UniversityChongqing 400042China CAS key {1. of Nutrition Metabolism and Food SafetyShanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Shanghai key {1. of Maternal Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchShanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China The department of Endovascular Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China key {1. of Regenerative Medicine of Ministry of Education Institute of Ageing and Regenerative MedicineJ

Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological *** a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly *** immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this ***,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory *** fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant.

关键词： aging senescence biomarker clock

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Fungal diversity notes 1.87-1.1.:taxonomic and phylogenetic contributions on genera and species of fungal taxa

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Fungal Diversity 2021年第6期111卷 1-335页

作者： Saranyaphat Boonmee Dhanushka N.Wanasinghe Mark S.Calabon Naruemon Huanraluek Sajini K.U.Chandrasiri Gareth E.B.Jones Walter Rossi Marco Leonardi Sanjay K.Singh Shiwali Rana Paras N.Singh Deepak K.Maurya Ajay C.Lagashetti Deepika Choudhary Yu-Cheng Dai Chang-lin Zhao Yan-Hong Mu Hai-Sheng Yuan Shuang-Hui He Rungtiwa Phookamsak Hong-Bo {2. María P.Martín Margarita Dueñas MTeresa Telleria Izabela L.Kałucka Andrzej M.Jagodziński Kare liimatainen Diana S.Pereira Alan J.L.Phillips Nakarin Suwannarach Jaturong Kumla Surapong Khuna Saisamorn Lumyong Tarynn B.Potter Roger G.Shivas Adam H.Sparks Niloofar Vaghefi Mohamed A.Abdel-Wahab Faten A.Abdel-Aziz Guo-Jie li Wen-Fei lin Upendra Singh Rajendra P.Bhatt Hyang Burm Lee Thuong T.T.Nguyen Paul M.Kirk Arun Kumar Dutta Krishnendu Acharya VVenkateswara Sarma M.Niranjan Kunhiraman C.Rajeshkumar Nikhil Ashtekar Sneha Lad Nalin N.Wijayawardene Darbe J.Bhat Rong-Ju Xu Subodini N.Wijesinghe Hong-wei Shen Zong-Long Luo Jing-Yi Zhang Phongeun Sysouphanthong Naritsada Thongklang Dan-feng Bao Janith V.S.Aluthmuhandiram Jafar Abdollahzadeh Alireza Javadi Francesco Dovana Muhammad Usman Abdul Nasir Khalid Asha J.Dissanayake Anusha Telagathoti Maraike Probst Ursula Peintner Isaac Garrido-Benavent lilla Bóna Zsolt Merényi Lajos Boros Bratek Zoltán JBenjamin Stielow Ning {2. Cheng-Ming Tian Esmaeil Shams Farzaneh Dehghanizadeh Adel Pordel Mohammad Javan-Nikkhah Teodor T.Denchev Cvetomir M.Denchev Martin Kemler Dominik Begerow Chun-ying Deng Emma Harrower Tohir Bozorov Tutigul Kholmuradova Yusufjon Gafforov Aziz Abdurazakov Jian-Chu Xu Peter E.Mortimer Guang-Cong Ren Rajesh Jeewon Sajeewa S.N.Maharachchikumbura Chayanard Phukhamsakda Ausana Mapook Kevin D.Hyde Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand CAS key {1. for Plant Biodiversity and Biogeography of East Asia(KLPB) Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China CIFOR-ICRAF China Program World Agroforestry(ICRAF)Kunming 650201YunnanPeople’s Republic of China Honghe Center for Mountain Futures Kunming Institute of BotanyHonghe CountyKunming 654400YunnanPeople’s Republic of China department of Botany and {1. College of ScienceKing Saud UniversityP.O.Box 2455Riyadh 11451Saudi Arabia Section Environmental Sciences Department MeSVAUniversity of L’Aquila67100 CoppitoAQItaly National Fungal Culture Collection of India(NFCCI) Biodiversity and Palaeobiology GroupMACS Agharkar Research InstituteG.G.Agarkar RoadPune 411004India Institute of microbiology School of Ecology and Nature ConservationBeijing Forestry UniversityBeijing 100083People’s Republic of China College of Biodiversity Conservation Southwest Forestry UniversityKunming 650224People’s Republic of China CAS key {1. of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110164People’s Republic of China university of the Chinese Academy of Sciences Beijing 100049People’s Republic of China Centre for Mountain Futures(CMF) Kunming Institute of BotanyKunming 650201YunnanPeople’s Republic of China department of Mycology Real Jardín Botánico-CSICPlaza de Murillo 228014 MadridSpain department of Algology and Mycology Faculty of Biology and Environmental ProtectionUniversity of LodzBanacha 12/1690-237ŁódźPoland Institute of Dendrology Polish Academy of SciencesParkowa 562-035 KórnikPoland Jodrell laboratory Royal Botanic GardensKewRichmond TW93DSSurreyUK Faculdade de Ciências Biosystems and Integrative Sciences Institute(BioISI)Universidade de LisboaCampo Grande1749-016 LisbonPortugal Dep

This article is the 1.th contribution in the Fungal Diversity Notes series,wherein 1.0} taxa from four phyla,ten classes,31.orders,69 fami{1.es,92.genera and three genera incertae sedis are treated,demonstrating worldwide and geographic *** taxa described and illustrated in the present study include three new genera,69 new species,one new com-bination,one reference specimen and 51.new records on new hosts and new geographical *** new genera,Cy{1.ndrotorula(Torulaceae),Scolecoleotia(Leotiales genus incertae sedis)and Xenovaginatispora({1.ndomycetaceae)are introduced based on distinct phylogenetic {1.neages and unique *** described species are Aspergillus lan-naensis,Cercophora dulciaquae,Cladophialophora aquatica,Coprinellus punjabensis,Cortinarius alutarius,***-latus,***,Coryneum fagi,Cruentomycena uttarakhandina,Cryptocoryneum rosae,Cyathus uniperidiolus,Cy{1.ndrotorula indica,Diaporthe chamaeropicola,Didymella azollae,Diplodia alanphil{1.psii,Dothiora coronicola,Efibula rodriguezarmasiae,Erysiphe sa{1.cicola,Fusarium queenslandicum,Geastrum gorgonicum,***,He{1.cosporium sexua{1.s,Helminthosporium chiangraiensis,Hongkongmyces kokensis,Hydrophilomyces hydraenae,Hygrocybe boertmannii,Hyphoderma australosetigerum,Hyphodontia yunnanensis,Khaleijomyces umikazeana,Laboulbenia divisa,Laboulbenia triarthronis,Laccaria popu{1.na,Lactarius pal{1.dozonarius,Lepidosphaeria strobe{1.i,Longipedicellata megafusiformis,Lophiotrema {1.ncangensis,Marasmius benghalensis,***,***,Mariannaea camel{1.ae,Mel-anographium smilaxii,Microbotryum polycnemoides,Mimeomyces digitatus,Minutisphaera thailandensis,Mortierella so{1.taria,Mucor harpa{1.,Nigrograna jinghongensis,Odontia huanrenensis,***,Paraconiothyrium ajrekarii,Par-afuscosporella niloticus,Phaeocytostroma yomensis,Phaeoisaria synnematicus,Phanerochaete hainanensis,Pleopunctum thailandicum,Pleurotheciella dimorphospora,Pseudochaetosphaeronema

关键词： 72.new taxa One new combination One reference specimen 51.new records Agaricomycetes Ascomycota Bartheletiomycetes Basidiomycota Dothideomycetes Eurotiomycetes Exobasidiomycetes Laboulbeniomycetes Leotiomycetes Mortierellomycetes Mortierellomycota Mucoromycetes Mucoromycota Phylogeny Sordariomycetes Taxonomy

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