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Science china({1.fe Sciences) 2005年第6期48卷 574-584页

作者： li feng1,{1.,{2., wang zhenfang1 & li jilun1 1. state key laboratory for agro-biotechnology and department of microbiology, china agricultural university, beijing 100094, china 2. department of Biology, Huaibei Coal Industry Teachers’ College, Huaibei 2.5000, china 1. state {1. {2. for agro-biotechnology and department of microbiology China Agricultural University 100094 Beijing China 2. {1. of Biology Huaibei Coal Industry Teachers’ College 235000 Huaibei China

A magnetosome deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1.was generated by mini-Tn5 transposon mutagenesis, and a 5045-bp fragment flanking mini-Tn5 in NM4 was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved six putative open reading frames (ORFs); the mini-Tn5 was inserted into ORF4. Functional complementary test indicated that the 5045-bp fragment was required for biosynthesis of mag-netosomes in M. gryphiswaldense MSR-1. The protein encoded by ORF4 had 2.% of identity with the chemotaxis protein CheYIII of Caulobacter crescentus CB1., and the protein encoded by ORF4 contained a conserved signal receiver domain that can receive the signal from the sensor partner of the bacterial two-component systems. It was suggested that the protein en-coded by ORF4 may take part in the signal transduction relating to biosynthesis of magneto-somes.

关键词： Magnetospirillum gryphiswaldense, magnetosome deleted mutant, gene cloning, functional analysis.

Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosome-deleted mutant NM2. of Magnetospirillum gryphiswaldense MSR-1

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Chinese Science Bulletin 2009年第9期54卷 1522-1528页

作者： li {1. li ying jiang wei wang zhenfang li jilun state {1. {2. for agro-biotechnology and department of microbiology China Agricultural University Beijing 100193 China School of life Science Anhui Key Laboratory of Plant Resources and Biology Huaibei Coal Industry Teachers' College Huaibei 235000 China

A magnetosome-deleted mutant NM2. of Magnetospirillum gryphiswaldense MSR-1.was generated by mini-Tn5 lacZ2.transposon mutagenesis, and a 3073-bp fragment flanking mini-Tn5 lacZ2.in NM2. was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved three putative ORFs; the mini-Tn5 lacZ2.was inserted into ORF1. Functional complementary test indicated that the 3073-bp fragment was required for biosynthesis of magnetosomes in M. gryphiswaldense MSR-1. The majority of proteins, which had homology with the protein encoded by ORF1. were the cation transporter. Transmembrane domain analysis showed that the protein encoded by ORF1.contained four trans-membrane domains. It may be a transmembrane protein. The protein encoded by ORF1.contained two putative conserved domains: COG0053 and PRK09509. The MMT1.and FieF, containing conserved do-mains COG0053 and PRK09509 too, were Fe2. transporter (cation diffusion facilitator superfamily). It was suggested that the protein encoded by ORF1.might take part in the magnetosomes biosynthesis as Fe2. transporter.

关键词： Tn5诱变科学研究缺失突变序列分析磁小体海洋迷你克隆

Functional analysis of hydrogenases and their effects on cell growth and magnetosome synthesis in Magnetospirillum gryphiswaldense

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Chinese Science Bulletin 2010年第13期55卷 1271-1277页

作者： BAN Jia jiang {1. {2. ying ZHANG YaoPing {2. jilun state {1. {2. for agrobiotechnology and College of Biological Sciences China Agricultural University Beijing 100193 China department of Bacteriology University of Wisconsin-Madison Madison W153706 USA

This study addressed the effect of hydrogen metabolism on cell growth and magnetosome synthesis in Magnetospirillum gryphiswaldense strain MSR-1. Two deletion mutants were generated: L{1.6, with single deletion of the hupL gene encoding H{1.uptake [NiFe] hydrogenase; and B{1.6, with double deletion of the hyaB gene encoding H{1.producing [NiFe] hydrogenase and the hupL gene. The wild-type and mutant strains were compared in terms of hydrogen uptake capability, hydrogen yield, growth rate, and iron uptake, and observed by transmission electron microscopy. Results indicate that HupSL protein is a specific H{1.uptake hydrogenase while HyaAB protein is a specific H{1.producing hydrogenase. In comparison to wild-type and B{1.6, L{1.6 released a greater quantity of H{1.under conditions that induce magnetosomes synthesis, and showed higher rates of growth and iron uptake. M. gryphiswaldense appears to regulate reducing power in vivo, via H{1.uptake hydrogenase and H{1.producing hy- drogenase, to promote iron absorption and magnetosome synthesis.

关键词：诱导合成细胞生长氢化酶电子显微镜观察氢气生产螺吸收能力缺失突变体

马铃薯ARM基因家族的全基因组鉴定及表达分析

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作物学报 2024年第6期50卷 1451-1466页

作者：刘震陈丽敏李志涛朱金勇王玮璐齐喆颖姚攀锋毕真真孙超白江平刘玉汇甘肃农业大学省部共建干旱生境作物学国家重点实验室甘肃兰州730070 甘肃农业大学农学院甘肃兰州730070 甘肃省教育厅甘肃兰州730030

ARM蛋白重复序列(Armadillo repeats)广泛存在于高等植物中,它们参与多种细胞过程,如信号转导、核转运以及对多种生物/非生物胁迫的响应。本研究在马铃薯(Solanum tuberosum L.)全基因组水平下鉴定出了54个马铃薯ARM基因家族成员(StARM... 详细信息

ARM蛋白重复序列(Armadillo repeats)广泛存在于高等植物中,它们参与多种细胞过程,如信号转导、核转运以及对多种生物/非生物胁迫的响应。本研究在马铃薯(Solanum tuberosum L.)全基因组水平下鉴定出了54个马铃薯ARM基因家族成员(StARMs),它们不均匀的分布在1.0}染色体上。根据其蛋白结构和系统发育特征,将54个StARMs分为3个亚家族。片段重复事件在马铃薯ARM基因家族的扩展中起主要作用。共线性分析发现,StARMs与番茄(Solanum lycopersicum)、拟南芥(Arabidopsis)、甘蓝(Brassica oleracea)、水稻(Oryza sativa)、玉米(Zea mays)分别有51.、1.对、2.对、6对和1.对直系同源基因,这些基因均在纯化选择下进化。RNA-seq数据分析发现,4个StARM基因在匍匐茎中特异表达,2.StARM基因在根和心皮中特异表达,1.StARM基因在块茎中特异表,还有一些StARM基因参与了马铃薯对生物/非生物胁迫的响应。此外,本研究对3个不同颜色马铃薯块茎组织(薯皮和薯肉)进行了RNA-seq测序,分析了54个StARMs在不同颜色马铃薯块茎组织中的表达模式,并利用qPCR分析了StARMs在3个不同颜色块茎杂交子代薯肉中的相对表达量,筛选出了4个可能参与马铃薯块茎花色素苷生物合成的候选基因。本研究为进一步了解StARM基因家族的特征,深入分析StARM基因在马铃薯抵御生物/非生物胁迫和调控块茎花色素苷生物合成中的功能提供了理论依据。

关键词：马铃薯 ARM基因家族生物/非生物胁迫花色素苷生物合成表达分析

Fungal diversity notes 1.87-1.1.:taxonomic and phylogenetic contributions on genera and species of fungal taxa

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Fungal Diversity 2021年第6期111卷 1-335页

作者： Saranyaphat Boonmee Dhanushka N.Wanasinghe Mark S.Calabon Naruemon Huanraluek Sajini K.U.Chandrasiri Gareth E.B.Jones Walter Rossi Marco Leonardi Sanjay K.Singh Shiwali Rana Paras N.Singh Deepak K.Maurya Ajay C.Lagashetti Deepika Choudhary Yu-Cheng Dai Chang-lin Zhao Yan-Hong Mu Hai-Sheng Yuan Shuang-Hui He Rungtiwa Phookamsak Hong-Bo {2. María P.Martín Margarita Dueñas MTeresa Telleria Izabela L.Kałucka Andrzej M.Jagodziński Kare liimatainen Diana S.Pereira Alan J.L.Phillips Nakarin Suwannarach Jaturong Kumla Surapong Khuna Saisamorn Lumyong Tarynn B.Potter Roger G.Shivas Adam H.Sparks Niloofar Vaghefi Mohamed A.Abdel-Wahab Faten A.Abdel-Aziz Guo-Jie li Wen-Fei lin Upendra Singh Rajendra P.Bhatt Hyang Burm Lee Thuong T.T.Nguyen Paul M.Kirk Arun Kumar Dutta Krishnendu Acharya VVenkateswara Sarma M.Niranjan Kunhiraman C.Rajeshkumar Nikhil Ashtekar Sneha Lad Nalin N.Wijayawardene Darbe J.Bhat Rong-Ju Xu Subodini N.Wijesinghe Hong-wei Shen Zong-Long Luo Jing-Yi Zhang Phongeun Sysouphanthong Naritsada Thongklang Dan-feng Bao Janith V.S.Aluthmuhandiram Jafar Abdollahzadeh Alireza Javadi Francesco Dovana Muhammad Usman Abdul Nasir Khalid Asha J.Dissanayake Anusha Telagathoti Maraike Probst Ursula Peintner Isaac Garrido-Benavent lilla Bóna Zsolt Merényi Lajos Boros Bratek Zoltán JBenjamin Stielow Ning {2. Cheng-Ming Tian Esmaeil Shams Farzaneh Dehghanizadeh Adel Pordel Mohammad Javan-Nikkhah Teodor T.Denchev Cvetomir M.Denchev Martin Kemler Dominik Begerow Chun-ying Deng Emma Harrower Tohir Bozorov Tutigul Kholmuradova Yusufjon Gafforov Aziz Abdurazakov Jian-Chu Xu Peter E.Mortimer Guang-Cong Ren Rajesh Jeewon Sajeewa S.N.Maharachchikumbura Chayanard Phukhamsakda Ausana Mapook Kevin D.Hyde Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand CAS key {1. for Plant Biodiversity and Biogeography of East Asia(KLPB) Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China CIFOR-ICRAF china Program World Agroforestry(ICRAF)Kunming 650201YunnanPeople’s Republic of China Honghe Center for Mountain Futures Kunming Institute of BotanyHonghe CountyKunming 654400YunnanPeople’s Republic of China department of Botany and {1. College of ScienceKing Saud UniversityP.O.Box 2455Riyadh 11451Saudi Arabia Section Environmental Sciences Department MeSVAUniversity of L’Aquila67100 CoppitoAQItaly National Fungal Culture Collection of India(NFCCI) Biodiversity and Palaeobiology GroupMACS Agharkar Research InstituteG.G.Agarkar RoadPune 411004India Institute of microbiology School of Ecology and Nature ConservationBeijing Forestry UniversityBeijing 100083People’s Republic of China College of Biodiversity Conservation Southwest Forestry UniversityKunming 650224People’s Republic of China CAS key {1. of Forest Ecology and Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110164People’s Republic of China university of the Chinese Academy of Sciences Beijing 100049People’s Republic of China Centre for Mountain Futures(CMF) Kunming Institute of BotanyKunming 650201YunnanPeople’s Republic of China department of Mycology Real Jardín Botánico-CSICPlaza de Murillo 228014 MadridSpain department of Algology and Mycology Faculty of Biology and Environmental ProtectionUniversity of LodzBanacha 12/1690-237ŁódźPoland Institute of Dendrology Polish Academy of SciencesParkowa 562-035 KórnikPoland Jodrell laboratory Royal Botanic GardensKewRichmond TW93DSSurreyUK Faculdade de Ciências Biosystems and Integrative Sciences Institute(BioISI)Universidade de LisboaCampo Grande1749-016 LisbonPortugal Dep

This article is the 1.th contribution in the Fungal Diversity Notes series,wherein 1.0} taxa from four phyla,ten classes,31.orders,69 fami{1.es,92.genera and three genera incertae sedis are treated,demonstrating worldwide and geographic *** taxa described and illustrated in the present study include three new genera,69 new species,one new com-bination,one reference specimen and 51.new records on new hosts and new geographical *** new genera,Cy{1.ndrotorula(Torulaceae),Scolecoleotia(Leotiales genus incertae sedis)and Xenovaginatispora({1.ndomycetaceae)are introduced based on distinct phylogenetic {1.neages and unique *** described species are Aspergillus lan-naensis,Cercophora dulciaquae,Cladophialophora aquatica,Coprinellus punjabensis,Cortinarius alutarius,***-latus,***,Coryneum fagi,Cruentomycena uttarakhandina,Cryptocoryneum rosae,Cyathus uniperidiolus,Cy{1.ndrotorula indica,Diaporthe chamaeropicola,Didymella azollae,Diplodia alanphil{1.psii,Dothiora coronicola,Efibula rodriguezarmasiae,Erysiphe sa{1.cicola,Fusarium queenslandicum,Geastrum gorgonicum,***,He{1.cosporium sexua{1.s,Helminthosporium chiangraiensis,Hongkongmyces kokensis,Hydrophilomyces hydraenae,Hygrocybe boertmannii,Hyphoderma australosetigerum,Hyphodontia yunnanensis,Khaleijomyces umikazeana,Laboulbenia divisa,Laboulbenia triarthronis,Laccaria popu{1.na,Lactarius pal{1.dozonarius,Lepidosphaeria strobe{1.i,Longipedicellata megafusiformis,Lophiotrema {1.ncangensis,Marasmius benghalensis,***,***,Mariannaea camel{1.ae,Mel-anographium smilaxii,Microbotryum polycnemoides,Mimeomyces digitatus,Minutisphaera thailandensis,Mortierella so{1.taria,Mucor harpa{1.,Nigrograna jinghongensis,Odontia huanrenensis,***,Paraconiothyrium ajrekarii,Par-afuscosporella niloticus,Phaeocytostroma yomensis,Phaeoisaria synnematicus,Phanerochaete hainanensis,Pleopunctum thailandicum,Pleurotheciella dimorphospora,Pseudochaetosphaeronema

关键词： 72.new taxa One new combination One reference specimen 51.new records Agaricomycetes Ascomycota Bartheletiomycetes Basidiomycota Dothideomycetes Eurotiomycetes Exobasidiomycetes Laboulbeniomycetes Leotiomycetes Mortierellomycetes Mortierellomycota Mucoromycetes Mucoromycota Phylogeny Sordariomycetes Taxonomy