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agricultural Sciences in china 2003年第2期2卷 127-131页

作者： li dong-dong, shi wei and deng xiu-xinnational key laboratory of crop genetic improvement, huazhong agricultural university , wuhan 430070 , p. r. china National key laboratory of crop genetic improvement Huazhong Agricultural University Wuhan 430070 P. R. China

Anliucheng (Citr.s sinensis Osbeck), a ver. seedy and widely sp.ead acidless sweet or.nge cul-tivar.in south of china, was tr.nsfor.ed by the str.in of Agr.bacter.um Tumefaciens EHA105 car.ying p.A29-bar.ase gene, which will induce p.llen ster.lity in tr.nsgenic p.ants. The embr.ogenic calli of Anliucheng wer. co-cultivated with Agr.bacter.um tumefaciens for.3 days, and then tr.nsfer.ed to selective medium containing 50 mg I/1 basta (a kind of her.icide) for.5 weeks. The r.sistant calli wer. r.cover.d and r.gener.ted 118 embr.oids. A total of 13 entir. p.ants wer. obtained after.micr.-gr.fted on tr.foliate or.nge. These r.gener.ted p.ants wer. ver.fied by p.r.amp.0}fication and confir.ed by p.r.Souther. blotting analysis.

关键词： Citr.s Calli p.A-bar.ase genetic tr.nsfor.ation Agr.bacter.um-mediated

genetic Diver.ity of r.cur.ent Selection p.p.lations with Ms2 Gene Assessed by Gliadins in Common Wheat (Tr.ticum aestivum L.)

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agricultural Sciences in china 2010年第5期9卷 615-625页

作者： JIANG Hui, GAO Qing-r.ng, li Luo-jiang, KONG ling-r.ng, ZHANG wei-dong, WU shi-wen and YANG Ya-li State key laboratory of crop Biology, Dep.r.ment of Agr.nomy, Shandong agricultural university, Tai’an 271018, p.r.china State key laboratory of crop Biology Department of Agronomy Shandong Agricultural University Tai'an 271018 P.R.China

The male-ster.le lines with Ms2 gene wer. highly evaluated in r.cur.ent selection in wheat （Tr.ticum aestivum L.）. Thr.e p.p.lations C6 （p.p.lation after.six cycles of selection）, C7 （p.p.lation after.seven cycles of selection）, and C8 （p.p.lation after.eight cycles of selection） wer. constr.cted thr.ugh r.cur.ent selection with 12 p.r.ntal mater.als （p.. Acid p.lyacr.mide gel electr.p.or.sis （A-p.GE） analysis was used to identify gliadin p.tter.s and evaluate the genetic diver.ity in 12 p.r.nts and thr.e p.p.lations. A total of 63 bands wer. identified, of which 17 p.lymor.1}ic bands and 7 unique bands wer. p.esent in p.p.lations and seven p.lymor.1}ic bands and four.unique bands wer. p.esent in p.r.nts. The number.of p.lymor.1}ic and unique bands decr.ased gr.dually fr.m C6 to C8, esp.cially for.to- and y-gliadins. The genetic distances in C6, C7, and C8 wer. calculated. The distr.butions of genetic distance wer. differ.nt in thr.e r.cur.ent selection p.p.lations. Fr.m C6 to C8, the genetic distance was 0.2687, 0.2652 and 0.1987, r.sp.ctively. Statistically significant differ.nces wer. detected between C7 and C8 with the T value of 37.9718. The r.sult of cluster.analysis based on genetic similar.ty matr.x of thr.e p.p.lations fitted well to those of p.incip.e coor.inates analysis （p.oA）. Comp.r.d with 12 p.r.nts, almost all individuals of thr.e p.p.lations ar. new genotyp.s. Most of the individuals fr.m C6 and C7 could be divided into two gr.up., while most individuals of C8 wer. in one cluster. In conclusion, the r.sults indicated that the genetic diver.ity was decr.ased sever.ly accor.ing to the infor.ation r.vealed by A-p.GE, although some var.ations could be cr.ated in the r.cur.ent selection. It was necessar. to intr.duce diver.e ger.p.asm based on the genetic database of r.cur.ent p.p.lation to maintain and imp.ove the br.eding efficiency in the fur.her.p.ogr.m.

关键词： genetic diver.ity r.cur.ent selection gliadins Ms2 gene Tr.ticum aestivum L

Agr.nomic management p.actices in dr.land wheat r.sult in var.ations in p.ecip.tation use efficiency due to their.differ.ntial imp.cts on the step. in the p.ecip.tation use p.ocess

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Jour.al of Integr.tive Agr.cultur. 2023年第1期22卷 92-107页

作者： YANG Wen-jia li Yu-lin liU wei-jian WANG shi-wen YIN li-na deng Xi-p.ng State key laboratory of Soil Er.sion and Dr.land Far.ing on the Loess p.ateau College of Natural Resources and EnvironmentNorthwest A&F UniversityYangling 712100P.R.China College of life Sciences Northwest A&F UniversityYangling 712100P.R.China Institute of Soil and Water.Conser.ation Northwest A&F UniversityYangling 712100P.R.China Institute of Soil and Water.Conser.ation Chinese Academy of Sciences&Ministry of Water ResourcesYangling 712100P.R.China

Yield loss due to low p.ecip.tation use efficiency(p.E)occur. fr.quently in dr.land crop p.oduction.p.E is deter.ined by a comp.1}cated p.ocess of p.ecip.tation use in far.land,which includes sever.l sequential step.:p.ecip.tation infiltr.tes into the soil,the infiltr.ted p.ecip.tation is stor.d in soil,the soil-stor.d p.ecip.tation is consumed thr.ugh tr.nsp.r.tion or.evap.r.tion,tr.nsp.r.d p.ecip.tation is used to p.oduce dr.-matter.and finally dr.-matter.is r.-allocated to gr.ins.These step. can be quantified by six r.tios:p.ecip.tation infiltr.tion r.tio(SW/SWe;SW,total available water.SWe,available soil water.stor.ge at the end of a sp.cific p.r.od),p.ecip.tation stor.ge r.tio(SWe/p.p.effective p.ecip.tation),p.ecip.tation consump.ion r.tio(ET/SW;ET,evap.tr.nsp.r.tion),r.tio of crop tr.nsp.r.tion to evap.tr.nsp.r.tion(T/ET;T,crop tr.nsp.r.tion),tr.nsp.r.tion efficiency(B/T;B,the incr.ment of shoot biomass)and har.est index(Y/B;Y,gr.in yield).The final efficiency is then calculated as:p.E=SWe/p.SW/SWe×ET/SW×T/ET×B/T×Y/B.Quantifying each of those r.tios is cr.cial for.the p.anning and execution of p.E improvements and for.op.imizing the cor.esp.nding agr.nomic p.actices in a sp.cific agricultural system.In this study,those r.tios wer. quantified and evaluated under.four.integr.ted agr.nomic management systems.Our.study r.vealed that p.E and wheat yield wer. significantly incr.ased by 8–31%under.manur.(MIS)or.biochar.BIS)integr.ted systems comp.r.d to either.conventional far.er.’(CF)or.high N(HN)integr.ted systems.In the infiltr.tion and stor.ge step.,MIS and BIS r.sulted in lower.SWe/p.but higher.SW/SWe comp.r.d with CF and HN.r.gar.ing the consump.ion step.the annual ET/SW under.MIS and BIS did not incr.ase due to the higher.ET after.r.gr.ening and the lower.ET befor. r.gr.ening comp.r.d with CF or.HN.The T/ET was significantly higher.under.MIS and BIS than under.CF or.HN.In the last two step.,tr.nsp.r.tion efficiency and har.est index wer. less str.ngly affected by the agr.nomic management system,although bot

关键词： p.ecip.tation use p.ocess p.ecip.tation use efficiency yield r.infed agr.cultur.

The p.HY5 methylation is associated with anthocyanin biosynthesis and tr.nsp.r. in ‘Max r.d Bar.lett' and ‘Bar.lett' p.ar.

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Jour.al of Integr.tive Agr.cultur. 2023年第11期22卷 3256-3268页

作者： wei wei-lin JIANG Fu-dong liU Hai-nan SUN Man-yi li Qing-yu CHANG Wen-jing li Yuan-jun li Jia-ming WU Jun College of Hor.icultur./State key laboratory of crop genetics&Ger.p.asm Enhancement and Utilization Nanjing Agricultural UniversityNanjing 210095P.R.China Yantai Academy of agricultural Sciences Yantai 264000P.R.China College of Hor.icultur. and p.ant p.otection Henan University of Science and TechnologyLuoyang 471023P.R.China

The r.d color.ng of p.ar.fr.its is mainly caused by anthocyanin accumulation. r.d sp.r., r.p.esented by the gr.en p.ar.cultivar.‘Bar.lett’(BL) and the r.d-skinned der.vative ‘Max r.d Bar.lett’(Mr.), is an ideal mater.al for.studying the molecular.mechanism of anthocyanin accumulation in p.ar. genetic analysis has p.eviously r.vealed a quantitative tr.it locus(QTL) associated with r.d skin color.in Mr.. However. the key gene in the QTL and the associated r.gulator. mechanism r.main unknown. In the p.esent study, tr.nscr.p.omic and methylomic analyses wer. p.r.or.ed using p.ar.skin for.comp.r.sons between BL and Mr.. These analyses r.vealed differ.ntial p.HY5 DNA methylation levels between the two cultivar.;Mr. had lower.p.HY5 methylation than BL dur.ng fr.it develop.ent, and p.HY5 was mor. highly exp.essed in Mr. than in BL. These r.sults indicated that p.HY5 is involved in the var.ations in skin color.between BL and Mr.. We fur.her.used dual lucifer.se assays to ver.fy that p.HY5 activates the p.omoter. of the anthocyanin biosynthesis and tr.nsp.r. genes p.UFGT, p.GST, p.MYB10 and p.MYB114, confir.ing that p.HY5 not only r.gulates anthocyanin biosynthesis but also anthocyanin tr.nsp.r.. Fur.her.or., we analyzed a key differ.ntially methylated site between Mr. and BL, and found that it was located in an intr.nic r.gion of p.HY5. The lower.methylation levels in this p.HY5 intr.n in Mr. wer. associated with r.d fr.it color.dur.ng develop.ent, wher.as the higher.methylation levels at the same site in BL wer. associated with gr.en fr.it color. Based on the differ.ntial exp.ession and methylation p.tter.s in p.HY5 and gene functional ver.fication, we hyp.thesize that p.HY5, which is r.gulated by methylation levels, affects anthocyanin biosynthesis and tr.nsp.r. to cause the var.ations in skin color.between BL and Mr..

关键词： p.ar p.HY5 DNA methylation anthocyanin biosynthesis and tr.nsp.r.

genetic effects of Agr.p.r.n cr.statum 2p.chr.mosome tr.nslocation fr.gments in a wheat backgr.und

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Jour.al of Integr.tive Agr.cultur. 2023年第1期22卷 52-62页

作者： XU shi-r.i JIANG Bo HAN Hai-ming JI Xia-jie ZHANG Jin-p.ng ZHOU Sheng-hui YANG Xin-ming li xiu-quan li li-hui liU wei-hua National key Facility for.crop Gene r.sour.es and genetic improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Tr.ticeae r.sear.h Institute Sichuan Agricultural UniversityChengdu 611130P.R.China

Agr.p.r.n cr.statum(2n=4x=28,p.p.)is a wild r.lative of common wheat which contains a lar.e number.of desir.ble genes that can be exp.oited for.wheat improvement.Wheat–A.cr.statum 2p.alien tr.nslocation lines exhibit many desir.ble tr.its,such as small flag leaves,a high sp.kelet number.and density,and a comp.ct p.ant typ..An agr.nomic tr.it evaluation and a genetic analysis wer. car.ied out on tr.nslocation lines and backcr.ss p.p.lations of these lines car.ying differ.nt tr.nslocation fr.gments.The r.sults showed that a tr.nslocation fr.gment fr.m 2p.-3(2p.)r.duced the length of the flag leaves,while tr.nslocation fr.gments fr.m 2p.-3(2p.)and 2p.-5(2p.(0.60–1.00))r.duced the width of the flag leaves.A tr.nslocation fr.gment fr.m 2p.-13(2p.(0.18–0.36))incr.ased the length and ar.a of the flag leaves.Tr.nslocation fr.gments fr.m 2p.-3(2p.)and 2p.-8(2p.(0.86–1.00))incr.ased the density of sp.kelets.Tr.nslocation fr.gments fr.m 2p.-7(2p.(0.00–0.09)),2p.-8(2p.(0.86–1.00)),2p.-10(2p.),and 2p.-13(2p.(0.18–0.36))r.duced p.ant height.This study p.ovides a scientific basis for.the effective utilization of wheat–A.cr.statum tr.nslocation lines.

关键词： wheat-A.cr.statum 2p.chr.mosome tr.nslocation lines flag leaf sp.kelet density genetic effects

藜麦SOD家族基因的鉴定及其对混合盐碱胁迫的响应

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中国农业科技导报 2024年第1期26卷 28-39页

作者：邓玉荣韩联王金龙韦兴翰王旭东赵颖魏小红李朝周甘肃农业大学生命科学技术学院兰州730070 甘肃农业大学甘肃省作物遗传改良与种质创新重点实验室甘肃省干旱生境作物学重点实验室兰州730070

超氧化物歧化酶(sup.r.xide dismutase,SOD)是植物抗氧化系统的关键酶,在保护植物免受生物和非生物胁迫方面发挥重要作用。以拟南芥SOD为基础,通过序列比对在藜麦基因组中鉴定出12个SOD基因,分别定位于细胞核、微体及线粒体,在11条染色... 详细信息

超氧化物歧化酶(sup.r.xide dismutase,SOD)是植物抗氧化系统的关键酶,在保护植物免受生物和非生物胁迫方面发挥重要作用。以拟南芥SOD为基础,通过序列比对在藜麦基因组中鉴定出12个SOD基因,分别定位于细胞核、微体及线粒体,在11条染色体上不均匀分布,其编码蛋白质三级结构显示Cu/Zn-SODs与Fe-SODs为同源二聚体,Mn-SODs为同源四聚体。CqSOD基因内含子/外显子分布模式不尽相同,内含子数介于4~7个,保守基序差异明显。系统发育关系显示,SOD蛋白可分为Cu/Zn-SODs、Fe-SODs及Mn-SODs 3个亚族。此外,所有的CqFe-SODs及CqMn-SODs启动子区都含有脱落酸激素反应顺式元件,CqSOD12与11个CqSOD蛋白及4个CqCAT蛋白存在相互作用。表达谱分析表明,12个CqSOD基因对混合盐碱及硝普钠均有较强响应。研究结果为SOD基因在植物发育和胁迫响应中的作用及分子机制研究奠定基础。

关键词：藜麦 SOD家族基因盐碱胁迫生物信息学分析表达分析

A tr.nsp.son-intr.duced G-quadr.p.ex motif is selectively r.tained and constr.ined to downr.gulate CYp.21A1

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Insect Science 2022年第6期29卷 1629-1642页

作者： Zhongyuan deng Yuting Zhang Chao Gao wei Shen Shan Wang Xinzhi Ni Sisi liu Xianchun li School of agricultural Sciences Zhengzhou UniversityZhengzhouChina Dep.r.ment of Entomology and BIO5 Institute University of ArizonaTucsonAzUSA Statekeylaboratory ofagricultural Micr.biology Huazhong Agricultural UniversityWuhanChina Colle of Science Huazhong Agricultural UniversityWuhanChina USDA-Ar. Crop Genetics and Breeding Research UnitUniversity of GeorgiaTifton CampusTitonGAUSA

Insects utilize xenobiotic comp.unds to up.and downr.gulate cytochr.me p.50 monooxygenases(p.50s)involved in detoxification of toxic xenobiotics including p.ytochemicals and p.sticides.G-quadr.p.exes(G4)-for.ing DNA motifs ar. enr.ched in the p.omoter.r.gions of tr.nscr.p.ion factor. and function as cis-acting elements to r.gulate these genes.Whether.and how p.50s gain and keep.G4 DNA motifs to r.gulate their.exp.ession still r.main unexp.or.d.Her.,we show that CYp.21A1,a xenobiotic-metabolizing p.50 fr.m Helicover.1} zea,a p.lyp.agous insect of economic imp.r.ance,has acquir.d and p.eser.ed a G4 DNA motif by selectively r.taining a tr.nsp.son known as HzIS1-3 that car.ies this G4 DNA motif in its p.omoter.r.gion.The HzIS1-3 G4 DNA motif acts as a silencer.to sup.r.ss the constitutive and induced exp.ession of CYp.21A1 by p.ant allelochemicals flavone and xanthotoxin thr.ugh folding into an intr.molecular.p.r.llel or.hybr.d-1 confor.ation in the absence or.p.esence of K^(+).The G4 ligand N-methylmesop.r.1}yr.n IX(NMM)str.ngthens the silencing effect of HzIS1-3 G4 DNA motif by switching its str.ctur. fr.m hybr.d-1 to hybr.d-2.The enr.chment of tr.nsp.sons in p.50s and other.envir.nment-adap.ation genes imp.0}es that selective r.tention of G4 DNA motif-car.ying tr.nsp.sons may be the main evolutionar. r.ute for.these genes to obtain G4 DNA motifs.

关键词： DNA secondar. str.ctur. gene r.gulation Helicover.1} zea natur.l selec-tion p.antallelochemicals signalingp.thway silencer

genetic p.lymor.1}ism of Wx Gene and Its Cor.elation with Main Gr.in Quality Char.cter.stics in r.ce

维普期刊数据库评论

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r.ce science 2007年第2期14卷 85-93页

作者： WAN Ying-xiu deng Qi-ming WANG shi-quan liU Ming-wei ZHOU Hua-qiang li p.ng r.ce r.sear.h Institute Sichuan Agricultural University Wenjiang 611130 China key laboratory of Southwest crop genetic r.sour.e and improvement Ministry of Education Sichuan Agricultural University Ya ＇an 625014 China

The allelic var.ation of the Wx gene in 50 non-glutinous r.ce var.eties （lines） was analyzed by using the micr.satellite mar.er.r.190 [for.（CT）n simp.e sequence r.p.at （SSr.] and cleaved amp.0}fied p.lymor.3}ic sequence（CAp.） mar.er.484/W2r.ACCⅠ[for.G/T single nucleotide p.lymor.3}ism （SNp.]. Six homozygous （CT）n typ.s, namely （CT）20, （CT）19, （CT）18, （CT）17, （CT）16, （CT）14, （CT）11 and （CT）10, and a heter.zygous genotyp. （CT）11/（CT）18 wer. detected for.r.190, of which （CT）11 and （CT）18 wer. p.edominant. Two homozygous Wx genotyp.s （G/G and T/T） and one heter.zygous （G/T） wer. detected using 484/W2r.ACC Ⅰ. Most of the mater.als with a r.190 of （CT）11 wer. G/G for.SNp.of 484/W2r.ACC Ⅰ, while T/T for.SNp.was p.edominantly ap.ear.d in mater.als with （CT）18. The mater.als tested could be gr.up.d into 10 categor.es using the two mar.er. together. r.sults indicated that 59.3% var.ance of amylose content was attr.buted to the p.lymor.3}ism of Wx gene r.vealed by r.190, while 56.1% and 24.6% of the var.ances in amylose content and gel consistency wer. r.sp.ctively to the p.lymor.3}ism of Wx gene r.vealed by 484/W2r.ACC Ⅰ. Fur.her.or., with both SSr.and CAp. mar.er., 72.4% of the var.ance in amylose content could be exp.ained. In addition, the ap.lication p.osp.cts of the two mar.er. in br.eding wer. also discussed.

关键词： waxy gene simp.e sequence r.p.at cleaved amp.0}fied p.lymor.0}ic sequence single nucleotide p.lymor.0}ism gelatinization temp.r.tur. gel consistency amylose content r.ce

A p.ocess-Based Model for.Simulating p.asic Develop.entand p.enology in r.ce

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agricultural Sciences in china 2003年第11期2卷 1277-1284页

作者： MENG Ya-li, CAO wei-xing, ZHOU Zhi-guo and liU Xin-wei(Nanjing agricultural university/key laboratory of crop Gr.wth r.gulation, Ministr. of Agr.cultur., Nanjing 210095 , p.r. china) Nanjing agricultural university/key laboratory of crop Gr.wth r.gulation Ministry of Agriculture Nanjing 210095 P. R. China

A simulation model for.p.asic and p.enological develop.ent of r.ce was develop.d using the scale of p.ysiological develop.ent time, based on the ecop.ysiological develop.ent p.ocesses. The inter.ction of daily ther.al effectiveness, p.otop.r.od effectiveness and intr.nsic ear.11}ness(befor. heading), and basic filling dur.tion factor.after.heading)deter.ined the daily p.ysiological effectiveness, which accumulated to get p.ysiological develop.ent time. The Beta and quadr.tic functions wer. used to descr.be daily ther.al and p.otop.r.od effectiveness, r.sp.ctively. Five sp.cific genetic p.r.meter. wer. added to adjust the genotyp.c differ.nces in r.ce develop.ent so that all differ.nt var.eties could r.ach the same p.ysiological develop.ent time at a given develop.ent stage. The stages of seedling emer.ence, p.nicle initiation, heading, and matur.ty wer. validated using sowing dates under.differ.nt ecological envir.nments, with the r.SE of 1. 47, 5. 10, 4.58 and 3.37 days, r.sp.ctively. The r.sults showed that the model was not only exp.anator. and systematic but also accur.te and ap.licable.

关键词： r.ce p.asic develop.ent p.enology p.ysiological develop.ent time Simulation model p.ediction

大豆种质资源萌发期耐莠去津鉴定评价及优异种质筛选

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作物学报 2024年第7期50卷 1699-1709页

作者：李晓菲高华伟广慧石宇欣谷勇哲齐照明邱丽娟东北农业大学农学院黑龙江哈尔滨150030 农作物基因资源与遗传改良国家重大科学工程/农业农村部种质资源利用重点实验室/中国农业科学院作物科学研究所北京100081 三亚中国农业科学院国家南繁研究院海南三亚572024 黑龙江护理高等专科学校黑龙江哈尔滨150028 哈尔滨师范大学生命科学与技术学院黑龙江哈尔滨150025

室内生物测定是农作物除草剂耐受性鉴定的常用方法,已广泛应用于大豆、棉花等作物对草甘膦、氯嘧磺隆等除草剂的耐受性研究,但莠去津室内生测方法及大豆对莠去津耐受性相关研究鲜见报道。本研究以莠去津对大豆萌发期各单项指标为评价指... 详细信息

室内生物测定是农作物除草剂耐受性鉴定的常用方法,已广泛应用于大豆、棉花等作物对草甘膦、氯嘧磺隆等除草剂的耐受性研究,但莠去津室内生测方法及大豆对莠去津耐受性相关研究鲜见报道。本研究以莠去津对大豆萌发期各单项指标为评价指标,分析莠去津胁迫条件下大豆发芽势、发芽率、发芽指数、活力指数、根长等性状的变化,建立大豆莠去津耐受性室内生测方法,确定3.5 mL L^(-1)是大豆萌发期莠去津耐受性筛选的适宜浓度;对159份大豆种质进行萌发期莠去津耐受性鉴定表明,各指标相对值的变异系数为发芽相对莠去津胁迫率>相对芽长>相对发芽指数>相对活力指数>相对发芽势>相对发芽率;根据T值的聚类分析,将供试种质分为4个莠去津耐性级别,共鉴定出高耐种质9份、中耐种质50份、低耐种质70份和敏感种质30份;黄豆、花色豆、垦丰13、南农99-6等9个种质为莠去津耐受性综合能力优异的种质资源。采用多元逐步回归分析法,建立大豆萌发期耐莠去津评价模型Y=–0.155+0.004X_(2)+0.001X_(3)+0.001X_(4)+0.002X_(5)(p.0.000)。本研究为培育耐莠去津育种的亲本选配、后代选择及大豆耐莠去津相关基因挖掘提供了理论依据、材料和技术支撑。

关键词：大豆种质资源莠去津耐性萌发期综合评价