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Production of Transgenic Anliucheng Sweet Orange(Citrus sinensis Osbeck) with Xa21 Gene for Potential Canker Resistance

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Journal of Integrative Agriculture 2014年第11期13卷 2370-2377页

作者： li ding-li XIAO Xuan GUO Wen-wu Key Laboratory of Horticultural Plant Biology Ministry of Education/College of Horticulture & Forestry Sciences Huazhong Agricultural University College of life and Environmental Sciences Gannan Normal University

Citrus canker, an epidemic quarantine disease caused by Xanthomonas axonopodis pv. citri, has brought a great damage in citrus production worldwide. Herein, a rice PRR （pattern recognition receptor） gene Xa21 together with GUS reporter gene and hygromycin phosphotransferase gene （HPT） was introduced into Anliucheng sweet orange （Citrus sinensis Osbeck） via Agrobacterium-mediated transformation of embryogenic callus. The transgenic calluses were screened on MT basal medium containing hygromycin （HYG） and detected by histochemical GUS staining. The transgenic plantlets were recovered through somatic embryogenesis pathway. The regenerated plantlets were accustomed to and maintained in the greenhouse. The transgene integration of recovered plantlets was identiifed by PCR and Southern blot hybridization. It showed that all the transgenic plantlets tested had undergone single copy integration, the expression of Xa21 in eight different transgenic lines detected by qRT-PCR can be divided into three grades, high for T5 and T6, middle for T4 and low for the rest. The tolerance to citrus canker disease of the three recovered transgenic lines T2, T4 and T6 was assessed by in vitro pin-puncture inoculation. The results showed that all the three transgenic lines conferred improved resistance to citrus canker bacterium infection and the T4 transgenic line displayed the highest resistance. The mechanism and feasibility of rice Xa21 in triggering innate immunity in citrus was brielfy discussed.

关键词： citrus canker Xa21 sweet orange pattern recognition receptors (PRRs)

A machine learning approach to TCAD model calibration for MOSFET

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Nuclear Science and Techniques 2023年第12期34卷 133-145页

作者： Bai‑Chuan Wang Chuan‑Xiang Tang Meng‑Tong Qiu Wei Chen Tan Wang Jing‑Yan Xu li‑li ding Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle and Radiation Imaging(Tsinghua University) Ministry of EducationBeijing 100084China State Key Laboratory of Intense Pulsed Radiation Simulation and Effect Xi’an 710024China

Machine learning-based surrogate models have significant advantages in terms of computing efficiency. In this paper, we present a pilot study on fast calibration using machine learning techniques. Technology computer-aided design(TCAD) is a powerful simulation tool for electronic devices. This simulation tool has been widely used in the research of radiation ***, calibration of TCAD models is time-consuming. In this study, we introduce a fast calibration approach for TCAD model calibration of metal–oxide–semiconductor field-effect transistors(MOSFETs). This approach utilized a machine learning-based surrogate model that was several orders of magnitude faster than the original TCAD simulation. The desired calibration results were obtained within several seconds. In this study, a fundamental model containing 26 parameters is introduced to represent the typical structure of a MOSFET. Classifications were developed to improve the efficiency of the training sample generation. Feature selection techniques were employed to identify important parameters. A surrogate model consisting of a classifier and a regressor was built. A calibration procedure based on the surrogate model was proposed and tested with three calibration goals. Our work demonstrates the feasibility of machine learning-based fast model calibrations for MOSFET. In addition, this study shows that these machine learning techniques learn patterns and correlations from data instead of employing domain expertise. This indicates that machine learning could be an alternative research approach to complement classical physics-based research.

关键词： Machine learning Radiation effects Surrogate model TCAD model calibration

Effect of Titanium,Antimony,Cerium and Carbon Nanotubes on the Morphology and Microhardness of Mg-based Icosahedral Quasicrystal Phase

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Journal of Materials Science & Technology 2010年第1期26卷 27-32页

作者： Zhifeng Wang, Weimin Zhao, Haipeng li, Jian ding, Yongyan li and Chunyong liang School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China School of Materials Science and Engineering Hebei University of Technology Tianjin 300130 China

For the first time, petal-like and spherical Mg-based icosahedral quasicrystal phase （I-phase） were obtained by introducing Ti, Sb, Ce and C nanotubes into Mg-Zn-Y alloy under normal casting conditions. The formation mechanism and stability criterion of spherical I-phase were discussed. The morphology and microhardness of I-phase and their determinants were studied in this paper. The results show that the different value of microhardness of I-phase could be attributed to the different kinds of the fourth component and its content, and its different innate characters. The final morphology of icosahedral quasicrystalline （IQC） is decided by the size of critical stable radius Rr, the content of the fourth component and degree of undercooling.

关键词： Quasicrystal Magnesium alloy Morphology Microhardness

Phenotype and mechanism analysis of plant dwarfing in pear regulated by abscisic acid

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Journal of Integrative Agriculture 2022年第5期21卷 1346-1356页

作者： liU Jian-long ZHANG Chen-xiao li Tong-tong liANG Cheng-lin YANG Ying-jie li ding-li CUI Zhen-hua WANG Ran SONG Jian-kun College of Horticulture Qingdao Agricultural UniversityQingdao 266109P.R.China Haidu College Qingdao Agricultural UniversityLaiyang 265200P.R.China

Close planting of dwarf varieties is currently the main cultivation direction for pear trees,and the screening of excellent dwarf varieties is an important goal for *** this study,the dwarfing pear variety‘601D’and its vigorous mutant‘601T’were used to show their biological characteristics and further explore the dwarfing mechanism in‘601D’.The biological characteristics showed that‘601D’had a shorter internode length,a shorter and more compact tree body,thicker and broader leaves,lower stomata density,larger stomata size(dimension),and higher photosynthetic *** biological characteristics of‘601T’showed notable *** results of endogenous hormone tests indicated that the contents of abscisic acid(ABA),ABA-glucosyl ester,and GA_(4) were higher in‘601D’,but the trans-zeatin content was *** transcriptomic analysis,significant differences were found in the biosynthetic and metabolic pathways of *** transcription factors such as bHLH,WRKY,and homeobox also participated in the regulation of plant *** therefore examined three hormones with obvious differences with‘601T’,and found that only ABA could induce‘601T’to return to a dwarfing plant ***,we conclude that the dwarfing of‘601D’is caused by an excessive accumulation of *** study provides a new theoretical basis for breeding dwarf varieties.

关键词： dwarf pear phenotype abscisic acid transcriptomic analysis

A Double Azole-bridged Dinuclear Copper(Ⅱ) Complex Containing 4-Pyridyl-NH-1,2,3-triazoles: Synthesis, Structure and Magnetism

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Chinese Journal of Structural Chemistry 2020年第5期39卷 918-926页

作者： ZHANG Xin JIANG Xiao-Feng CHEN Yun-Zhou CHEN Yun-Feng ding li-li JIA li-Hui Key Laboratory for Green Chemistry Process of Ministry of Education School of Chemistry and Environmental EngineeringWuhan Institute of TechnologyWuhan 430073China Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Function Molecules Hubei UniversityWuhan 430062China

A new dinuclear copper(Ⅱ)compound[Cu2(CH3COO)2(L)2(H2O)2]·4H2O(1,HL=4-pyridyl-NH-1,2,3-triazole)has been synthesized and ***-crystal X-ray diffraction showed that complex 1 crystallizes in monoclinic space group P21/c with a=0.8427(1),b=1.0981(1),c=1.3610(1)nm,β=93.364(4)°,V=1.2573(1)nm^3,Mr=643.56,Z=2,Dc=1.700 g/cm^3,μ=1.760 mm-1,R=0.0376,wR=0.0981,and possesses a planar Cu2N4 six-membered ring,in which both Cu(Ⅱ)ions are bridged by two 1,2,3-triazole *** neighboring dinuclear molecules are bound by strong intermolecular hydrogen bonds to create 1-D chains expanding along the b axis,which further form a 3-D supramolecular *** susceptibility measurement indicated the presence of weak antiferromagnetic interactions within the *** best fit using spin Hamiltonian yields the magnetic coupling constant J=–14.82 cm^-1 above 30 *** magnetic data are compared to those obtained for related double diazine bridged dinuclear copper(Ⅱ)complexes and magnetostructural correlations are discussed.

关键词： dinuclear Cu(Ⅱ)complexes 4-pyridyl-NH-1,2,3-triazole magnetic properties magneto-structural correlation

Different phenotypes of monocytes in patients with newonset mild acute pancreatitis

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World Journal of Gastroenterology 2017年第8期23卷 1477-1488页

作者： Man-li Zhang Yan-Fang Jiang Xin-Rui Wang li-li ding Hong-Juan Wang Qing-Qing Meng Pu-Jun Gao Department of Hepatology and Gastroenterology The Second Part of First Hospital of Jilin University Department of Central Laboratory The Second Part of First Hospital of Jilin University Key Laboratory of Zoonosis Research Ministry of EducationJilin University Department of Hepatology The First Hospital of Jilin University Department of Intensive Care Unit The First Hospital of Jilin University

AIM To evaluate the numbers of different subsets of monocytes and their associations with the values of clinical measures in mild acute pancreatitis(MAP) *** The study included one group of 13 healthy controls and another group of 24 patients with new-onset MAP. The numbers of different subsets of monocytes were examined in these two groups of subjects by flow cytometry. The concentrations of plasma interleukin(IL)-10 and IL-12 were determined by cytometric bead array. The acute physiology and chronic health evaluation(APACHE) II scores of individual patients were evaluated, and the levels of plasma C-reactive protein(CRP) as well as the activities of amylase and lipase were measured. RESULTS In comparison with that in the controls, significantly increased numbers of CD14+CD163-, CD14+CD163-MAC387+ M1 monocytes, but significantly reduced numbers of CD14+CD163+IL-10+ M2 monocytes were detected in the MAP patients(P < 0.01 or P < 0.05). Furthermore, significantly higher levels of plasma IL-10 and IL-12 were observed in the MAP patients(P < 0.01 for all). More importantly, the levels of plasma CRP were positively correlated with the numbers of CD14+CD163-(R = 0.5009, P = 0.0127) and CD14+CD163-MAC387+(R = 0.5079, P = 0.0113) M1 monocytes and CD14+CD163+CD115+ M2 monocytes(R = 0.4565, P = 0.0249) in the patients. The APACHE II scores correlated with the numbers of CD14+CD163+CD115+(R = 0.4581, P = 0.0244) monocytes and the levels of plasma IL-10(R = 0.4178, P = 0.0422) in the MAP patients. However, there was no significant association among other measures tested in this population. CONCLUSION Increased numbers of CD14+CD163- and CD14+ CD163-MAC387+ monocytes may contribute to the pathogenesis of MAP, and increased numbers of CD14+CD163+CD115+ monocytes may be a biomarker for evaluating the severity of MAP.

关键词： Mild acute pancreatitis Monocyte Cytokine Acute physiology and chronic health evaluation II score C-reactive protein

Host Cell Protein C9orf9 Promotes Viral Proliferation via Interaction with HSV-1 UL25 Protein

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Virologica Sinica 2011年第3期26卷 171-180页

作者： Ying Zhang Yan-mei li Long-ding liu li Jiang Ma Ji Rui-ju Jiang Lei Guo Yun liao Qi-han li Institute of Medical Biology Chinese Academy of Medicine Science ＆ Peking Union Medical College Kunming650118 China）

In light of the scarcity of reports on the interaction between HSV-1 nucleocapsid protein UL25 and its host cell proteins,the purpose of this study is to use yeast two-hybrid screening to search for cellular proteins that can interact with the UL25 protein.C9orf69,a protein of unknown function was *** interaction between the two proteins under physiological conditions was also confirmed by biological experiments including co-localization by fluorescence and immunoprecipitation.A preliminary study of the function of C9orf69 showed that it promotes viral *** studies showed that C9orf69 did not influence viral multiplication efficiency by transcriptional regulation of viral genes,but indirectly promoted proliferation via interaction with UL25.

关键词： Herpes simplex virus(HSV-1) UL25 C9orf69 Proliferation

Politicizing the queue： The social, political and cultural symbolism of hair in Qing dynasty and young republican China

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Sino-US English Teaching 2010年第12期7卷 53-58页

作者： ding li-li Sydney Institute of Language ＆ Commerce Shanghai University Shanghai 201800 China

The human body, such as hair, serves as a prism through which historical and cultural contexts are effectively refracted. Despite its historical and cultural significance, the role of hair, however, remains curiously a marginalized subject among the renewed interests on the body in the academic fields. In this paper, the author attempts to politicize the queue from 3 perspectives： maintaining a certain prescribed hairstyle is a top-down gesture to construct national conformity; the boundary between Manchu and Han is invoked and reinvented through the battles surrounding the queue politic in late Qing and early Republic; the widespread debate between keeping the queue and cutting the queue at the turn of the 20th century epitomizes the haunting rhetoric of traditionalism and modernism pursuit of modernity in China.

关键词： queue hairstyle politic social cultural symbolism Chinese Confucius Qing dynasty Republican China Manchu Han

Impact of neutron-induced displacement damage on the single event latchup sensitivity of bulk CMOS SRAM

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Chinese Physics B 2017年第1期26卷 542-546页

作者： Xiao-Yu Pan Hong-Xia Guo Yin-Hong Luo Feng-Qi Zhang li-li ding Jia-Nan Wei Wen Zhao Northwest Institute of Nuclear Technology Xi＇an 710024 China

Since the displacement damage induced by the neutron irradiation prior has negligible impact on the performance of the bulk CMOS SRAM, we use the neutron irradiation to degrade the minority carrier lifetime in the regions responsible for latchup. With the experimental results, we discuss the impact of the neutron-induced displacement damage on the SEL sensitivity and qualitative analyze the effectiveness of this suppression approach with TCAD simulation.

关键词： displacement damage neutron irradiation single event latchup TCAD simulation