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P-glycoprotein regulating biphasic insulin secretion in rat pancreatic beta cells

Chinese Medical Journal 2009年第21期122卷 2587-2592页

作者： TANG Yun-zhao li dai-qing SUN Fu-jun li li YU De-min Key Lab of Hormone and Development (Tianjin and Ministry of Health of the People's Republic of China) Institution of Endociinology Tianjin Metabolic Diseases Hospital Tianjin Medical University Tianjin 300070 China

Background A 65-kD mdrl （multi-drug resistance protein 1, P-glycoprotein）-Iike protein has been suggested to be the regulatory protein to the chloride channel protein 3 （CIC-3） mediating insulin granules acidification and release in mouse pancreatic beta cells. But the protein has not been deeply investigated. In this study, we identified existence of the 65-kda protein in rat islets and preliminarily explored its biological functions. Methods Total RNAs of rat kidneys served as positive controls, and pancreas, islets and INS-1 cells were extracted for reverse-transcript PCR （RT-PCR）, respectively. The cDNAs were run with specific primers selected from the mRNA of abcblb encoding P-glycoprotein. All PCR products were visualized in agarose gel electrophoresis and sequenced. Homogenates of rat islets and INS-1 cells were applied to SDS-PAGE. P-glycoprotein was detected by a specific monoclonal antibody, C219. Biphasic insulin release was measured in static incubations of rat islets with radioimmunology assay. Results Compared with positive control, expression of the P-glycoprotein mRNA segments were detected in the islets, INS-1 cells and pancreas. Sequence analysis confirmed that the PCR products were matched with mRNA of P-glycoprotein. A 65-kda protein was recognized by the antibody in the islets homogenate but not in that of INS-1 cells in Western-blotting. Instead, the homogenate of INS-1 cells contained a 160-kda protein recognized by the antibody. Insulin secretion of rat islets were stimulated by high glucose （16.7mmol/L）, and showed biphasic curve during 60-minute incubation. After co-incubation with cyclosporine A （CsA）, specific inhibitor to P-glycoprotein, the second phase of insulin secretion was reduced significantly while the first phase was not influenced. Conclusions The 65-kda protein expressed in rat islets is most likely a mini-P-glycoprotein. It may play a key role regulating biphasic insulin release.

关键词： pancreatic beta cell islet P-glycoprotein biphasic insulin secretion

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Long-term ecological compensation policies and practices in China:Insights from the Three Rivers Headwaters Area

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Ecological Economy 2015年第2期8卷 175-184页

作者： li Fen ZHANG lin-bo li dai-qing GONG Bin ZHU Fu-jing Chinese Research Academy of Environmental Sciences State Environmental Protection Key Laboratory of Regional Ecological Processes and Functions Assessment State Key Laboratory of Environmental Criteria and Risk Assessment Jiangxi Province Key Lab for Digital Land East China Institute of Technology

Using the Three Rivers Headwaters Area in China as a pilot project, this study has investigated the effectiveness of the ecological compensation policies and practices have implemented in this area over the past decade. Major issues have encountered during the implementation process, including the formidable extent of grassland degradation, the comprehensive nature of measures needed to restore the ecosystem, and the time needed to achieve these goals. These issues are discussed, and remedial measures proposed. They include: drafting regulations giving the Three Rivers Headwaters Area ecological protection, setting aside funds for ecological compensation and establishing a national park, using an ecological assets accounting methodology for financial reporting purposes, designing a science-based approach for conducting the livestock husbandry population migration, enhancing the oversight capacity for all aspects of the ecological compensation process, and making an overall plan to promote the harmonious development of this area together with other regions in qinghai Province.

关键词： ecological compensation ecological assets accounting national park supervise Three Rivers Headwaters Area

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Primary Selection of MicroRNA Acting on Classical Swine Fever Virus in Swine Umbilical Vein Endothelial Cells

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畜牧兽医学报 2011年第S1期 30-33页

作者： HOU Bo,ZHANG Yan-ming,NING Peng-bo,KANG Kai,DENG li,dai Chen,TANG qing-hai (College of Veterinary Medicine,Northwest A & F University,Yangling 712100,China)

The aim of the study was to identify the microRNAs that act on classical swine fever virus (CSFV) in swine umbilical vein endothelial cells (SUVECs). The 3’-and 5’-untranslated regions ( UTR) of CSFV were cloned and then inserted into psiCHECH TM-2 plasmids carrying Firefly and Renilla luciferases reporter genes; microRNAs that acted with CSFV were predicted by bio-informatics analysis; then the recombinant plasmids and inhibitors of the predicted microRNA were co-transfected into SUVECs. The activities of Firefly and Renilla luciferases were detected by luminometry. The PCR products of the CSFV 5’-UTR (373 bp) and 3’-UTR (252 bp) were detected by electrophoresis on 1% agarose gels. Four microRNAs (ssc-miR-let7c, ssc-miR-106a, ssc-miR-18, ssc-miR-139) were screened out and evaluated. The CSFV 3’-UTR is the important target site of microRNA in SUVECs. The four microRNAs mentioned above had different inhibitory effects on the CSFV 3’-UTR, of which the ssc-miR-18 played the most important role.

关键词： classical swine fever virus microRNA SUVECs

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Cloaking an object in a half space without reflection

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Chinese Physics B 2013年第5期22卷 295-298页

作者：郭鹏斐李杜戴晴付云起 College of Electronic Science and Engineering National University of Defense Technology

A novel cloaking scheme to hide an object in a half space from electromagnetic （EM） detection without reflection is firstly presented. The proposed cloaking scheme contains a couple of matching strips, which consist of an isotropic material layer and an anisotropic UPML layer, located right under the bottom surface of a semi-cylindrical cloaking shell. Simple expressions for the material parameters of the cloaking scheme are derived. Numerical simulations are also performed, and a good cloaking effect is achieved. The cloaking scheme is effective to hide the local object with strong scattering characters placed on mobile carders, such as the radar antenna system on an aircraft.

关键词： invisibility cloak half space matching strips reflection

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Effects and mechanism of glucagon-like peptide-1 on injury of rats cardiomyocytes induced by hypoxia-reoxygenation

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Chinese Medical Journal 2008年第21期121卷 2134-2138页

作者： XIE Yun WANG Shao-xin SHA Wei-wei ZHOU Xue WANG Wei-tin HAN li-pin li dai-qing YU De-min Department of Diabetic Neurology Metabolic Disease Hospital Tianjin Medical University Tianjin 300070 China Department Of Endocrinology Beijing Changping District Hosoital Beijing 102200 China Department of Endocrinology Chinese People's Liberation Army 252 Hospital Baoding Hebei 071000 China

Background Although the insulinotropic role of glucagon-like peptide-1 （GLP-1） in type 2 diabetes mellitus has been substantiated, its role in cardioprotection remains largely unknown. This study aimed to determine the effects of GLP-1 on injury of rats cardiac myocytes induced by hypoxia-reoxygenation （H/R） and the possible mechanisms. Methods The cultured neonatal rats cardiac myocytes were randomly divided into seven groups： the normal control group, the H/R group, the GLP-1＋H/R group, the GLP-1＋H/R＋UO126 （the p42/44 mitogen-activated protein kinase （MAPK） inhibitor） group, the GLP-1＋H/R＋LY294002 （phosphatidylinositol 3-kinase （PI3K） inhibitor） group, the H/R＋UO126 group, and the H/R＋LY294002 group. The lactate dehydrogenase （LDH） activity, apoptosis rate of cardiac myocytes, and caspase-3 activity were detected after the injury of H/R. Results Compared with the normal control group, the activity of LDH, cardiac myocyte apoptosis rate, and caspase-3 activity all increased significantly in the H/R group （P 〈0.01）. Compared with the H/R group, these three indices all decreased in the H/R＋GLP-1 group （P 〈0.01）. However, the changes of LDH activity, apoptosis rate, and caspase-3 activity were inhibited by LY294002 and UO126 respectively. Conclusions GLP-1 can directly act on cardiac myocytes and protect them from H/R injury mainly by inhibiting their apoptosis. Its mechanism may be through the PI3K-Akt pathway and the MAPK signaling pathway.

关键词： glucagon-like peptide-1 hypoxia-reoxygenation apoptosis caspase-3 MAP kinase PI3K/Akt

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多传感器融合下船舶机电系统多发故障信号监测

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舰船科学技术 2024年第5期46卷 149-152页

作者：李烈熊戴立庆福建船政交通职业学院福建福州350007

为了提高船舶维护效率,提出一种多传感器融合下船舶机电系统多发故障信号监测方法。根据故障状态下的信号频率,使用小波变换法提取故障信号特征参数作为蚁群算法优化BP神经网络输入,实现多发故障诊断,并通过DS证据理论完成多传感器数据... 详细信息

为了提高船舶维护效率,提出一种多传感器融合下船舶机电系统多发故障信号监测方法。根据故障状态下的信号频率,使用小波变换法提取故障信号特征参数作为蚁群算法优化BP神经网络输入,实现多发故障诊断,并通过DS证据理论完成多传感器数据融合,得出故障诊断结果。实验结果表明,该方法可通过多传感器融合判断出船舶机电系统故障类型,即使一种传感器出现故障也不影响诊断效果,诊断船舶机电系统多发故障平均准确率高达97.02%,能够实现较为精准的船舶机电系统多发故障监测。

关键词：多传感器融合船舶机电系统故障监测小波变换蚁群算法 DS证据理论

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Primary Selection of MicroRNA Acting on Classical Swine Fever Virus in Swine Umbilical Vein Endothelial Cells

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畜牧兽医学报 2011年第B12期42卷 30-33页

作者： HOU Bo ZHANG Yan-ming NING Peng-bo KANG Kai DENG li dai Chen rANG qing-hai （College of Veterinary Medicine Northwest A ＆ F University Yangling 712100 China）

The aim of the study was to identify the microRNAs that act on classical swine fever virus (CSFV) in swine umbilical vein endothelial cells (SUVECs). The 3'-and 5'-untranslated regions ( UTR) of CSFV were cloned and then inserted into psiCHECH TM-2 plasmids carrying Firefly and Renilla luciferases reporter genes; microRNAs that acted with CSFV were predicted by bio-informatics analysis; then the recombinant plasmids and inhibitors of the predicted microRNA were co-transfected into SUVECs. The activities of Firefly and Renilla luciferases were detected by luminometry. The PCR products of the CSFV 5'-UTR (373 bp) and 3'-UTR (252 bp) were detected by electrophoresis on 1% agarose gels. Four microRNAs (ssc-miR-let7c, ssc-miR-106a, ssc-miR-18, ssc-miR-139) were screened out and evaluated. The CSFV 3'-UTR is the important target site of microRNA in SUVECs. The four microRNAs mentioned above had different inhibitory effects on the CSFV 3'-UTR, of which the ssc-miR-18 played the most important role.

关键词：脐静脉内皮细胞猪瘟病毒血管内皮细胞 microRNA 荧光素酶报告基因小分子RNA 重组质粒电泳检测

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托里消毒散精简方对糖尿病皮肤溃疡大鼠创面愈合的影响及其机制

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中草药 2016年第9期47卷 1560-1566页

作者：李玉珠张晓娜王颖陈莉明常柏李代清潘从清天津医科大学卫生部激素与发育重点实验室天津医科大学代谢病医院天津300070

目的探讨托里消毒散精简方全方及其拆方影响糖尿病皮肤溃疡大鼠创面愈合的作用机制。方法 200只雄性SD大鼠随机分为对照组、模型组、托里消毒散精简方全方(黄芪、当归、白芷、皂角刺)组、托法(黄芪、当归)组、透法(白芷、皂角刺)组,采用... 详细信息

目的探讨托里消毒散精简方全方及其拆方影响糖尿病皮肤溃疡大鼠创面愈合的作用机制。方法 200只雄性SD大鼠随机分为对照组、模型组、托里消毒散精简方全方(黄芪、当归、白芷、皂角刺)组、托法(黄芪、当归)组、透法(白芷、皂角刺)组,采用尾iv链脲佐菌素(STZ)50 mg/kg制备糖尿病模型,同时背部打孔法制备糖尿病皮肤溃疡大鼠模型。各组分别ig给予托里消毒散精简方全方及其拆方的浸膏,对照组和模型组ig等量生理盐水,连续给药14 d,分别于创伤后5、8、11、14 d评价创面溃疡愈合情况。采用HE染色观察创面肉芽组织形态学改变;免疫组织化学法检测创面组织血管生成素受体2(Tie-2)、血管生成素1(Ang-1)、血管生成素2(Ang-2)在肉芽组织中的定位表达;Western blotting法检测创面肉芽组织中Tie-2、Ang-1、Ang-2的蛋白表达水平。结果创伤后5、8、11、14 d,各药物组大鼠创面愈合率均高于模型组(P<0.05);创伤后5 d,透法组愈合率高于托法组和全方组(P<0.05);创伤后8、11、14 d,全方组愈合率高于托法组和透法组(P<0.05);全方组各时间点肉芽组织中Ang-1、Tie-2蛋白表达水平均高于模型组(P<0.05),Ang-2蛋白表达水平均低于模型组(P<0.05);创伤后5、8 d,透法组Ang-2蛋白表达水平明显下调(P<0.05);创伤后11、14 d,托法组Ang-1、Tie-2蛋白表达水平上调(P<0.05)。结论托里消毒散精简方全方可能通过上调创面肉芽组织中Ang-1、Tie-2的蛋白表达水平,下调Ang-2的蛋白表达水平促进创面愈合。

关键词：托里消毒散托法透法糖尿病皮肤溃疡血管生成

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寄主植物根浸提液对肉苁蓉种子萌发及吸器形成的影响

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应用生态学报 2020年第7期31卷 2264-2270页

作者：何秀丽李代晴冯茹乔海莉刘赛徐荣徐常青陈君中国医学科学院北京协和医学院药用植物研究所北京100193

利用滤纸培养皿法研究藜科植物梭梭和甜菜根水浸提和乙醇浸提两种浸提液对肉苁蓉种子萌发和吸器形成的影响。结果表明:水和乙醇浸提的根浸提液对肉苁蓉种子萌发和吸器形成无明显作用;两种根醇浸提液添加10 mg·kg^-1赤霉素(GA3)处理的... 详细信息

利用滤纸培养皿法研究藜科植物梭梭和甜菜根水浸提和乙醇浸提两种浸提液对肉苁蓉种子萌发和吸器形成的影响。结果表明:水和乙醇浸提的根浸提液对肉苁蓉种子萌发和吸器形成无明显作用;两种根醇浸提液添加10 mg·kg^-1赤霉素(GA3)处理的肉苁蓉种子萌发率均提高了10倍以上,但与只加赤霉素处理组(GA3对照)无显著差异。在根浸提液中添加1 mg·kg^-1氟啶酮(FL)处理的肉苁蓉种子萌发率与FL对照组无显著差异,其中甜菜根水浸提液处理组肉苁蓉种子萌发率最高,达39.4%。与根浸提液中添加赤霉素处理仅能使萌发率提高不同,寄主根浸提液中添加FL后,萌发肉苁蓉种子芽管上均有吸器形成,梭梭根醇浸提处理的吸器形成率最高,达16.2%。梭梭根醇浸提液中同时添加GA3和FL,肉苁蓉种子萌发率可提高到52.3%,但吸器形成率与浸提液中添加FL处理无差异;FL对照仅有6.7%的萌发肉苁蓉种子形成吸器,显著低于梭梭根醇浸提液添加FL处理组。不同处理的肉苁蓉种子吸器形成位置和形态有差异,添加寄主根浸提液处理的吸器大多出现在芽管顶端,多个乳头状凸起成爪状;未添加寄主根浸提液的FL对照组吸器出现位置大多在芽管底部或顶端出现分叉。研究证明,乙醇浸提和水浸提两种方式都能从寄主根中提取出促进肉苁蓉种子吸器形成的物质但对促进种子萌发作用不明显;GA3和FL可显著提高肉苁蓉种子的萌发率,但萌发肉苁蓉种子吸器的形成受寄主根浸提液中某些物质的影响。

关键词：梭梭根浸提液萌发率吸器

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P-糖蛋白过表达对INS-1和大鼠胰岛β细胞分化的影响

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天津医药 2019年第1期47卷 23-26页

作者：陈亚高雅莉李代清天津医科大学代谢病医院卫生部激素与发育重点实验室天津市代谢性疾病重点实验室300070

目的探讨P-糖蛋白过表达对大鼠胰岛β细胞系INS-1细胞和大鼠胰岛β细胞分化的影响。方法分别构建INS-1细胞(Ad-abcb1b腺病毒感染)和SD大鼠(Ins2-abcb1b随机插入)P-糖蛋白过表达模型。以阴性对照病毒和野生型SD大鼠为对照组,P-糖蛋白过... 详细信息

目的探讨P-糖蛋白过表达对大鼠胰岛β细胞系INS-1细胞和大鼠胰岛β细胞分化的影响。方法分别构建INS-1细胞(Ad-abcb1b腺病毒感染)和SD大鼠(Ins2-abcb1b随机插入)P-糖蛋白过表达模型。以阴性对照病毒和野生型SD大鼠为对照组,P-糖蛋白过表达组和转基因SD大鼠为实验组,萃取细胞和胰岛总RNA,定量PCR分别检测INS-1细胞和胰岛组织Abcb1b、Pdx1、Ins1、Ins2、Mafa、Mafb、Ngn3、Nkx6.1、Nkx2.2、Neurod1、Pax6等基因的表达。结果与对照组相比,P-糖蛋白过表达后,INS-1细胞Ngn3、Mafb转录水平升高(P0.05)。结论 P-糖蛋白过表达可能使INS-1细胞去分化,而对胰岛β细胞的分化无明显影响。

关键词： P糖蛋白胰岛素分泌细胞细胞分化糖尿病再生

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