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Transcriptome analysis reveals the effects of alkali stress on root system architecture and endogenous hormones in apple rootstocks

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Journal of Integrative Agriculture 2019年第10期18卷 2264-2271页

作者： liU Xuan liANG Wei li Yu-xing li Ming-jun MA Bai-quan liU Chang-hai MA Feng-wang li cui-ying State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of Horticulture Northwest A&F University

Soil alkalinity is a major factor that restricts the growth of apple roots.To analyze the response of apple roots to alkali stress, the root structure and endogenous hormones of two apple rootstocks, Malus prunifolia (alkali-tolerant) and Malus hupehensis (alkali-sensitive), were compared. To understand alkali tolerance of M. prunifolia at the molecular level, transcriptome analysis was performed. When plants were cultured in alkaline conditions for 15 d, the root growth of M. hupehensis with weak alkali tolerance decreased significantly. Analysis of endogenous hormone levels showed that the concentrations of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in M. hupehensis under alkali stress were lower than those in the control. However, the trend for IAA and ZR in M. prunifolia was the opposite. The concentration of abscisic acid (ABA) in the roots of the two apple rootstocks under alkali stress increased, but the concentration of ABA in the roots of M. prunifolia was higher than that in M. hupehensis. The expression of IAA-related genes ARF5, GH3.6, SAUR36, and SAUR32 and the Cytokinin (CTK)-related gene IPT5 in M. prunifolia was higher than those in the control, but the expression of these genes in M. hupehensis was lower than those in the control. The expression of ABA-related genes CIPK1 and AHK1 increased in the two apple rootstocks under alkali stress, but the expression of CIPK1 and AHK1 in M. prunifolia was higher than in M. hupehensis. These results demonstrated that under alkali stress, the increase of IAA, ZR, and ABA in roots and the increase of the expression of related genes promoted the growth of roots and improved the alkali tolerance of apple rootstocks.

关键词： alkali stress apple rootstock endogenous hormone root architecture transcriptome analysis

Comprehensive evaluation of tolerance to alkali stress by 17 genotypes of apple rootstocks

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Journal of Integrative Agriculture 2016年第7期15卷 1499-1509页

作者： ZHANG Kun-xi WEN Tian DONG Jun MA Feng-wang BAI Tuan-hui WANG Kun li cui-ying State Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture Northwest A&F University College of Horticulture Henan Agricultural University Institute of Pomology Chinese Academy of Agricultural Sciences

Alkaline soils have a great inlfuence on apple production in Northern China. Therefore, comprehensive evaluations of toler-ance to such stress are important when selecting the most suitable apple rootstocks. We used hydroponics culturing to test 17 genotypes of apple rootstocks after treatment with 1：1Na2CO3and NaHCO3. When compared with the normaly grown controls, stressed plants produced fewer new leaves, and had shorter roots and shoots and lower fresh and dry weights after 15 d of exposure to alkaline conditions. Their root/shoot ratios were also reduced, indicating that the roots had been severely damaged. For al stressed rootstocks, electrolyte leakage （EL） and the concentration of malondialdehyde （MDA） increased while levels of chlorophyl decreased. Changes in root activity （up or down）, as wel as the activities of peroxidase （POD）, superoxide dismutase （SOD）, and catalase （CAT） were rootstock-dependent, possibly relfecting their differences in alkali tolerance. Using alkali injury index （AI）, adversity resistance coefifcients （ARC）,cluster analysis, and evaluation of their physiological responses, we classiifed these 17 genotypes into three groups：（1） high tolerance： Hubeihaitang, Wushanbianyehaitang, Laoshanhaitang Ls2, Xiaojinbianyehaitang, and Fupingqiuzi; （2） moderate tolerance： Pingyitiancha, Laoshanhaitang Ls3, Hubeihaitang A1, Deqinhaitang, Balenghaitang, Maoshandingzi, Shandingzi, and Xinjiangyepingguo; or （3） low tolerance： Pingdinghaitang, Hongsanyehaitang, Xiaojinhaitang, and Sanyehaitang. These results wil signiifcantly contribute to the selection of the most suitable materials for rootstocks with desired levels of tolerance to alkali stress.

关键词： alkali stress apple rootstock alkali tolerance

器官捐献者家属心理健康状况及与特质正念的关系

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中国临床心理学杂志 2024年第3期32卷 680-684,694页

作者：谢文照李晋芳熊欢李翠英龙琦桢杨飞龙中南大学湘雅三医院长沙410013 中南大学生命科学学院长沙410013 医学信息研究湖南省普通高等学校重点实验室(中南大学) 长沙410013

目的:本研究旨在调查器官捐献者家属的心理健康状况,并检验器官捐献者家属的特质正念与心理健康状况之间的关联,以期为针对捐献者家属制定心理干预方案提供依据。方法:通过器官捐献协调员点对点联系器官捐献者家属,遵循自愿同意原则,采... 详细信息

目的:本研究旨在调查器官捐献者家属的心理健康状况,并检验器官捐献者家属的特质正念与心理健康状况之间的关联,以期为针对捐献者家属制定心理干预方案提供依据。方法:通过器官捐献协调员点对点联系器官捐献者家属,遵循自愿同意原则,采用事件影响量表修订版、正念注意觉知量表、病人健康问卷抑郁量表、广泛性焦虑量表对器官捐献者家属进行问卷调查,使用SPSS 26.0对调查结果进行分析。结果:共收到155份有效问卷,器官捐献者家属中创伤后应激症状的检出率为61.94%,抑郁的检出率为40.65%,焦虑的检出率为30.97%,特质正念的得分是63.67±12.76。器官捐献者家属的特质正念水平与其创伤应激症状(B=-0.068,P<0.05)、抑郁(B=-0.066,P<0.05)、焦虑(B=-0.075,P<0.05)呈负相关关系。结论:公民逝世后器官捐献者家属心理健康问题亟需关注,创伤后应激症状、抑郁和焦虑发生率较高。器官捐献者家属的特质正念与其心理健康密切相关。

关键词：器官捐献心理健康正念创伤后应激症状抑郁

Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress

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Journal of Integrative Agriculture 2019年第6期18卷 1280-1294页

作者： GENG Da-li LU li-yuan YAN Ming-jia SHEN Xiao-xia JIANG li-juan li Hai-yan WANG li-ping YAN Yan XU Ji-di li cui-ying YU Jian-tao MA Feng-wang GUAN Qing-mei State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYangling 712100P.R.China College of Information Engineering Northwest A&F UniversityYangling 712100P.R.China

Water deficit is one of the main limiting factors for apple growth and production.Root architecture plays an important role in drought tolerance of plants.However,little is known about the molecular basis of root system in apple trees under drought.In this study,we compared root morphology of two widely used rootstocks of apple(R3 and Malus sieversii)under drought.Our results suggested that M.sieversii is more tolerant to drought than R3,since M.sieversii had a higher ratio of root to shoot as well as root hydraulic conductivity under long-term drought conditions.We then performed whole-genome transcriptomic analysis to figure out the molecular basis of root responses in M.sieversii under drought.It was found that genes involved in transcription regulation,signaling or biosynthesis of hormones,and oxidative stress were differentially expressed under drought.Consistent with the gene expression profile,roots of M.sieversii had higher activities of peroxidase(POD)and superoxide dismutase(SOD)under drought,as well as higher content of abscisic acid(ABA)and lower content of auxin.Taken together,our results revealed the physiological and transcriptomic analyses of M.sieversii roots in response to drought.

关键词： Malus sieversii root architecture drought stress RNA-seq

Acute Effects of Low Temperatures and Cold Waves on Elderly Infectious Pneumonia Mortality—Jinan City,Shandong Province,China,2014-2022

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China CDC weekly 2024年第5期6卷 77-82,I0002-I0005页

作者： Huiyun Chang Mingjun li ying Wang liangliang cui Tiantian li Jinan Municipal Center for Disease Control and Prevention Jinan Municipal Center for Disease Control and Prevention Affiliated to Shandong UniversityJinan CityShandong ProvinceChina Chinese Filed Epidemiology Training Program Chinese Center for Disease Control and PreventionBeijingChina China CDC Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijingChina

Summary What is already known about this topic?The mortality rate due to pneumonia varies depending on the infectious agents present in a low-temperature environment.What is added by this report?This study aimed to examine the relationship between low temperatures and cold waves and the risk of mortality from infectious pneumonia in the elderly.The findings indicate a significant increase in the risk of infectious pneumonia.

关键词： environment Shandong finding

Ultimate treatment for a patient with severe traumatic brain injury without intake for 192 hours after Wenchuan massive earthquake

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Chinese Medical Journal 2009年第1期122卷 113-116页

作者： GU Jian-wen ZHOU Hu-tian YANG Tao CHENG Jing-min QU Yan li cui-ying li Qing-jie LU Ji-yun CHENG Shou-long liU Jian-jun KUANG Yong-qin HUANG Hai-dong YANG li-bin HE Wei-qi ZHAO Kai SONG li-xin ZHENG Xiao-dong liU Shu-xin SUN Yan HE Hong-lian WU Jiang liU Xu-sheng LU Min Department of Neurosurgery General Hospital of the People's liberation Army Chengdu Military Region Chengdu Sichuan 610083 China Department of Blood Transfusion Hospital of the People's liberation Army Chengdu Military Region Chengdu Sichuan 610083 China Ministry of Health People's Liberation Army General Logistics Department Beijing 100842 China Department of Emergency 255 Hospital of the People's Liberation Army Tangshan Hebei 063000 China

At 14：28 （2：28 pm） on May 12, 2008, a magnitude 8 earthquake, which was a rare event, occurred inSichuan Province of China and led to deaths of thousands of people. In the Longmen Mountain fault zone, which is located 40 miles from the earthquake center at Wenchuan, the Chinese military used a variety of treatment methods to rescue the injured and successfully treated a victim with severe traumatic brain injury （TBI） who survived without food or water intake for 192 hours. Two hundred and twenty-four hours after the earthquake, the patient underwent craniotomy and removal of an intracerebral hematoma. The injury to the patient was severe and the rescue time was the longest for this earthquake. Detailed information is provided in this report.

关键词： earthquake brain injury shock

Differentiation Between Amyotrophic Lateral Sclerosis and Mimics Using Quantitative Analysis of Fasciculation with Muscle Ultrasound

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Chinese Medical Sciences Journal 2023年第4期38卷 265-272页

作者： Jing Fan Yi li Jing-Wen Niu Nan Hu Yu-Zhou Guan li-ying cui Ming-Sheng liu Department of Neurology Peking Union Medical College HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730 Department of Neurology The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310000

Objective To determine the diagnostic accuracy of the intensity of fasciculation evaluated by muscle ultrasound in the differential diagnosis of amyotrophic lateral sclerosis(ALS).Methods We prospectively recruited patients who had ALS and neuropathy-radiculopathy attending Peking Union Medical College Hospital from 2017 to 2020.Healthy adults from a community were recruited as healthy controls.Muscle strength was assessed using the Medical Research Council(MRC)scale.At the first visit to the hospital,patients were assessed for maximal grade of fasciculations,total fasciculation score,and fasciculation grade in 16 muscle groups of bilateral upper and lower limbs using ultrasonography.The sensitivity and specificity of maximal grade of fasciculations,total fasciculation score,and fasciculation grade for the diagnosis of ALS were assessed by receiver operating characteristic analyses.Results The percentage of limb muscles with a maximal fasciculation grade higher than grade 2 in ALS patients and neuropathy-radiculopathy patients was 84.9%and 9.8%,respectively(χ^(2)=172.436,Plimb muscles detected,the total fasciculation score[median(interquartile range)]was 29(15,41)in ALS patients and 3(0,8)in neuropathy-radiculopathy patients(Z=9.642,Plimb muscles may be helpful for differential diagnosis of ALS.

关键词： amyotrophic lateral sclerosis neuropathy-radiculopathy muscle ultrasonography fasciculation muscle strength

Effects of repetitive transcranial magnetic stimulation on adenosine triphosphate content and microtubule associated protein-2 expression after cerebral ischemia-reperfusion injury in rat brain

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Chinese Medical Journal 2008年第14期121卷 1307-1312页

作者： FENG Hong-lin YAN li cui li-ying Department of Neurology First Hospital of Harbin Medical University Harbin Heilongjiang 150001 China Department of Neurology China-Japan Friendship Hospital of Beijing Beijing 100029 China Department of Neurology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China

Background Repetitive transcranial magnetic stimulation （rTMS） research has mainly been focused on the therapeutic effect of psychiatric disorders and Parkinson＇s disease. A few studies have shown that rTMS might protect against delayed neuronal death induced by transient ischemia, enhance long-term potentiation in ischemic conditions and affect regional brain blood flow and metabolism. The aim of this study was to determine the effects of repetitive transcranial magnetic stimulation （rTMS） on adenosine triphosphate （ATP） content and microtubule associated protein-2 （MAP-2） expression in rat brain after middle cerebral artery occlusion （MCAO）/reperfusion. Methods To study the effects of different timecourses of rTMS on ATP content and MAP-2 expression, 90 rats were randomly divided into three groups （30 rats in each group）. To study the effects of multiple rTMS parameters on ATP content and MAP-2 expression, the rats in each group were further divided into six subgroups （five rats each）. The rats were sacrificed at 1-hour, 24-hour and 48-hour intervals after reperfusion, and the brain tissues were collected for the detection of ATP and MAP-2. Results rTMS could significantly increase ATP content and MAP-2 expression in the left brain following ischemic insult （P 〈0.01） and different rTMS parameters had different effects on the ATP level and the MAP-2 expression in the left striatum. A high-frequency rTMS played an important role in MAP-2 expression and ATP preservation. Conclusions This study revealed that rTMS induced significant increase of ATP content and MAP-2 expression in the injured area of the brain, suggesting that the regulation of both ATP and MAP-2 may be involved in the biological mechanism of the effect of rTMS on neural recovery. Therefore, rTMS may become a potential adjunctive therapy for ischemic cerebrovascular disease.

关键词： transcranial magnetic stimulation repetitive cerebral ischemia-reperfusion injury adenosine triphosphate microtubule associated protein-2

Additives strategies to improving cycling stability of li metal batteries by modifying electrode electrolyte interphases

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Progress in Natural Science:Materials International 2024年第2期34卷 338-344页

作者： Yuan li Pengfei Huang Chunyu cui Xiaoyuan Ma li Chen Zekun li linhai li Yuhong Tian School of Biological and Chemical Engineering Guangxi University of Science and Technology School of College of Chemistry and Chemical Engineering Hunan University Isky Future Energy Institute

li metal batteries（LMBs） have attracted much attention due to the ultra-high theoretical capacity of the li metal anode（LMA）.However,the uncontrollable dendrites growth and low coulombic efficiency hinder their practical application.Here,we explore 3,5-difluoropyridine（2F-BD） as a novel electrolyte additive to enable highperformance LMBs.The 2F-BD additive participates in and modifies the solvation structure of li ions by reducing the coordinated number of PF6-at the electrode surface,leading to the formation of a liF and li3N-rich solid electrolyte interphase（SEI）.The liF component increases the robustness of SEI and suppresses the formation of li dendrites,while the li3N component facilitates the transportation and reaction kinetics of li ions.As a result,the li‖li symmetric cell presents a stable cycling performance of up to 500 h at a current density of 1 mA cm-2.After coupling with liFePO4cathode,the obtained full cell achieves high specific capacities of 106.06 mA h g-1and 84.98 mA h g-1at 2.55 mA cm-2and 5.10 mA cm-2,respectively,and maintains a high capacity retention of84.0% after 1000 cycles at 0.13 mA cm-2,with an average cycling CE of 99.58%.

关键词： Electrolyte additive li dendrites li metal batteries liF and li3N-Rich electrode-electrolyte interphase liFePO4 cathode