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Nuclear magnetic resonance based metabolomic differentiation of different Astragali Radix

Chinese Journal of Natural Medicines 2017年第5期15卷 363-374页

作者： li ai-ping li Zhen-Yu QU Ting-li QIN Xue-Mei DU Guan-Hua Modern Research Center for Traditional Chinese Medicine Shanxi University Shanxi 030006 China College of Chemistry and Chemical Engineering Shanxi University Shanxi 030006 China School of Pharmaceutical Science Shanxi Medical University Shanxi 030006 China Institute of Materia Medica Chinese Academy of Medical Sciences Beijing 100050 China

Astragali Radix(AR) is one of the most popular herbal medicines in traditional Chinese medicine(TCM). Wild AR is believed to be of high quality, and substitution with cultivated AR is frequently encountered in the market. In the present study, two types of ARs(wild and cultivated) from Astragalus membranaceus(Fisch.) Bge. and A. membranaceus var. mongholicus(Bge.) Hsiao, growing in different regions of China, were analyzed by NMR profiling coupled with multivariate analysis. Results showed that both could be differentiated successfully and cultivation patterns or growing years might have greater impact on the metabolite compositions than the variety; the metabolites responsible for the separation were identified. In addition, three extraction methods were compared and the method(M1) was used for further analysis. In M1, the extraction solvent composed of water, methanol, and chloroform in the ratio of 1 : 1 : 2 was used to obtain the aqueous methanol(upper layer) and chloroform(lower layer) fractions, respectively, showing the best separation. The differential metabolites among different methods were also revealed. Moreover, the sucrose/glucose ratio could be used as a simple index to differentiate wild and cultivated AR. Meanwhile, the changes of correlation pattern among the differential metabolites of the two varieties were found. The work demonstrated that NMR-based non-targeted profiling approach, combined with multivariate statistical analysis, can be used as a powerful tool for differentiating AR of different cultivation types or growing years.

关键词： Nuclear Magnetic Resonance Chemical profiling Astragali Radix Cultivation patterns Variety Regions

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Determination of five neurotransmitters in the rat brain for the study of the hypnotic effects of Ziziphi Spinosae Semen aqueous extract on insomnia rat model by UPLC-MS/MS

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Chinese Journal of Natural Medicines 2019年第7期17卷 551-560页

作者： YAN Yan li Qiang DU Hui-Zhi SHEN Chen-Xi li ai-ping PEI Xiang-ping DU Chen-Hui QIN Xue-Mei Modern Research Center for Traditional Chinese Medicine Shanxi University Taiyuan 030006 China School of Chinese Materia Medica Shanxi University of Traditional Chinese Medicine Taiyuan 030619 China

Ziziphi Spinosae Semen(ZSS) has been used for treatment of insomnia in China for centuries. To reveal the influence of insomnia on the levels of the neurotransmitters including serotonin(5-HT), glutamic acid(Glu),γ-aminobutyric acid(GABA),noradrenaline(NE) and dopamine(DA), and to study the role of ZSS aqueous extract in the treatment of insomnia, an UPLC-ESIMS/MS method was developed and validated for simultaneous determination of five neurotransmitters in the rat brain. The brain samples were pretreated by one-step direct protein precipitation with acetonitrile. The analytes were detected in positive mode with multiple reaction monitoring(MRM) and the procedure was completed in less than 10 min. The method showed a good linearity(R^2 >0.9967) with the other validation parameters were within acceptance range. The results indicated that the concentration of 5-HT, GABA and DA is significantly lower(P liorate the symptoms of insomnia by significantly changing the levels of the neurotransmitter parameters mentioned above. The data obtained in this study demonstrate that ZSS aqueous extract could ameliorate the symptoms of insomnia by modulating the levels of monoamines and amino acid neurotransmitters in the brain.

关键词： Ziziphi Spinosae Semen Neurotransmitters UPLC-MS/MS Hypnotic effects

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Potential quality evaluation approach for the absolute growth years’ wild and transplanted Astragali Radix based on anti-heart failure efficacy

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Chinese Journal of Natural Medicines 2020年第6期18卷 460-471页

作者： li Ke ZHANG Rui li Shu-Ying liU Yue-Tao li ai-ping liU Xiao-Jie DU Guan-Hua QIN Xue-Mei Modern Research Center for Traditional Chinese Medicine Shanxi UniversityTaiyuan 030006China College of Chemistry and Chemical Engineering Shanxi UniversityTaiyuan 030006China Key Laboratory of Chemical Biology and Molecular Engineering Ministry Shanxi UniversityTaiyuan 030006China Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

The quality of Astragali Radix(AR) was closely related to the growth period. However, the current commodity grades of AR were only divided by diameter but not directly related to the growth period, which leads to the contradiction between the grade standard and the quality evaluation index. Therefore, solving this problem will be the key for the quality evaluation of AR. The present study established a potential quality evaluation approach for the absolute growth years’ wild Astragali Radix(WAR) and transplanted Astragali Radix(TAR) based on the chemical components and anti-heart failure efficacy through adopting a bare-handed sections approach to rapidly identify the growth years of WAR. In this study, the absolute growth years of WAR were obtained by identifying the growth rings of 1–6 growth years root through the methods. The contents of flavonoids and saponins in 2–6 growth years’ WAR were determined by HPLC-UV-ELSD. The contents of 12 chemical components and the anti-fatigue failure effects of WAR(4-year-old)and TAR were compared on rat models of heart failure induced by doxorubicin. Meanwhile, NMR-based untargeted metabolomics studies were performed to investigate the regulative effects of WAR and TAR. The result shows that the numbers of growth rings were consistent with the actual growth periods of AR. The HPLC-UV-ELSD determination indicated that the content of total flavonoids in WAR was significantly higher than that in TAR. Pharmacodynamics analysis revealed that the effects of WAR on cardiac function parameters(EF, FS and LVIDs), contents of serum CK and BNP were superior to those of TAR. 13 metabolites of heart were identified that had a higher rate of change in WAR group than TAR. Overall, a rapid identification method for the growth years of WAR was established, and the fact that WAR were significantly better than TAR in the heart failure rats was first proved in the paper. This study provided a scientific basis for establishing a novel commodity specification and grade of AR for clinical rationa

关键词： Astragali Radix Growth rings Chemical analysis Anti-heart failure

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电流热效应实验装置的两次改进

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中小学实验与装备 2024年第1期34卷 42-45页

作者：李爱萍邱娟湖北省襄阳市教育科学研究院 441002 湖北省襄阳市教育装备中心 441002

焦耳定律是初中物理教学的重要内容,是描述电流通过导体产生热量的基本规律,做好演示实验是理解焦耳定律的关键。笔者经过长期的研究和实践,利用日常生活中常见的器材制成了焦耳定律演示仪,该仪器在一定程度上解决了原实验器材密封性不... 详细信息

焦耳定律是初中物理教学的重要内容,是描述电流通过导体产生热量的基本规律,做好演示实验是理解焦耳定律的关键。笔者经过长期的研究和实践,利用日常生活中常见的器材制成了焦耳定律演示仪,该仪器在一定程度上解决了原实验器材密封性不好、漏气等问题,让实验操作更加便捷,实验效果更明显,且方便推广。

关键词：焦耳定律电流实验改进

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Metabolomics combined with serum pharmacochemistry discovering effective compounds of Fangji Huangqi Tang against nephrotic syndrome

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中国药理学与毒理学杂志 2019年第10期 817-818页

作者： YANG liu li ai-ping CUI Wen-bo QIN Xue-mei Modern Research Center for Traditional Chinese Medicine of Shanxi University College of Chemistry and Chemical Engineering of Shanxi University

OBJECTIVE An integrative strategy was established to discover the effective compounds and their therapeutic targets using Fangji Huangqi Tang(FHT) aiming at inhibiting nephrotic syndrome(NS) as a case study. METHODS The adriamycin-induced nephropathy rat model was evaluated by histopathology analysis and urine protein. The serum biomarkers(pathological marker)related to NS model were characterized by metabolomics, and the metabolites which could be regulated to normal levels after administration with FHT were defined as FHT-regulated biomarkers(effective marker). Moreover, the potential effective compounds were identified by comparison of drug serum between control and model rats. Furthermore, they were further screened based on the correlation analysis between effective marker with the potential effective compounds. At the same time, the potential target of effective ingredients was found by network pharmacology technology. RESULTS The results of serum metabonomics showed that 15 metabolites,including 3-Hydroxybutyric acid, L-phenylalanine and linolenic acid, were associated with renal damage. Among them, 6 effective markers were uric acid, 2-methylbutyrylcarnitine and 10-HDA. Metabolic pathway analysis showed that, phenylalanine, tyrosine and tryptophan biosynthesis, linoleic acid metabolism, phenylalanine metabolism, sphingolipid metabolism were the key pathway associated with NS. The correlation analysis showed that nine constituents such as fan Ghinoline,atractylenolide Ⅲ, cycloastragenol, glycyrrhetinic acid were recognized as effective compounds, whose potential protein targets participated in MAPK signaling pathway,Gn RH signaling pathway and aldoaterone-regulated sodium reabsorption. CONCLUSION This study provides a methodological reference for the study of the efficacy material base of other traditional Chinese medicineand also provides an important basis for the target of FHT against NS.

关键词： Fangji Huangqi Tang metabolomics serum pharmacochemistry effective components nephrotic syndrome

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基于LC-MS代谢组学技术的肾病综合征潜在分型及进展生物标志物的研究

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药学学报 2024年第6期59卷 1779-1786页

作者：张庆瑜王倩张星星郭松佳李爱平山西大学中医药现代研究中心山西太原030006 地产中药功效物质研究与利用山西省重点实验室山西太原030006 山西大学化学生物学与分子工程教育部重点实验室山西太原030006 山西省人民医院山西太原030012

肾病综合征(nephrotic syndrome,NS)具有多种分型且发病机制和病理类型多样,临床上主要依赖血清生化进行诊断,而具体分型的鉴定必须通过肾穿刺进行活检,患者依从性较差。因此,寻找一种可无创、快速反映肾病综合征分型及疾病进展的方法... 详细信息

肾病综合征(nephrotic syndrome,NS)具有多种分型且发病机制和病理类型多样,临床上主要依赖血清生化进行诊断,而具体分型的鉴定必须通过肾穿刺进行活检,患者依从性较差。因此,寻找一种可无创、快速反映肾病综合征分型及疾病进展的方法对于临床诊断具有重要意义。本研究运用LC-MS代谢组学技术结合受试者工作特征曲线(receiver operating characteristic,ROC)和多元线性回归分析筛选鉴定可反映肾病综合征分型及疾病进展的潜在生物标志物。结果显示,依据正交偏最小二乘判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)模型中的变量VIP>1、P0.5筛选出区分膜性肾病(membranous nephropathy,MN)与IgA肾病(IgA nephropathy,IgAN)的5个潜在分型标志物包括吲哚乙酸、异亮氨酸脯氨酸、DL-吲哚-3-乳酸、D-苯丙氨酸和L-色氨酸。进一步以肾小球滤过率(estimated glomerular filtration rate,eGFR)为因变量,采用多元线性回归分析明确了可反映膜性肾病进展为尿毒症(uremia)的潜在进展标志物,包括丙氨酰亮氨酸、9-癸酰肉碱、葡萄糖酸、辛基甘氨酸和癸二酸,而IgA肾病进展为尿毒症的潜在进展标志物为丙氨酰亮氨酸、9-癸酰肉碱、辛基甘氨酸和癸二酸。该研究为肾病综合征潜在分型及进展生物标志物的发现提供理论依据,也为其他进展性疾病潜在生物标志物的发现提供方法参考。该方案经山西省人民医院伦理委员会同意[(2020)省医科伦审字第30号]。

关键词：潜在分型/进展标志物尿液代谢组学 ROC分析多元线性回归分析

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Effects of Hybridizing Berkshire Boar with Landrace × Yorkshire Sow on the Carcass Traits and Meat Quality of Hybrid Progeny

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Animal Husbandry and Feed Science 2013年第5期5卷 236-239页

作者： SHEN Xue-lin li ai-ping PU Xue-dong Management Office of Animal Husbandry and Veterinarian of Kaili City in Guizhou Province

[ Objective] The research aimed to provide theoretical basis for further popularization and application of Berkshire Pig in Southeast Guizhou. [ Method] The carcass traits and meat quality of Berkshire（B） × Landrace（L） × Yorkshire（Y）, Duroc（D） × Landrace（L） x Yorkshire（Y） and Lanclrace （L） xYorkshire（Y） were determined by slaughter test. And the application effect of Berkshire in the minority areas was analyzed. [Result] From the carcass traits, the hybridized pig of Berkshire（B） x Landrace（L） ×Yorkshire（Y） had higher slaughter rate and lower lean meat percentage, having extremely significant difference with Landrace （L） × Yorkshire （Y） and significant difference with Durec （D） × Landrace （L） × Yorkshire（Y）. The meat indices of Berkshire（B） × Landrace（L） ×Yorkshire（Y） were better than that of Landrace （L） ×Yorkshire（Y） and Duroc （D）× Landrace（L） ×Yorkshire（Y）. The eye muscle area of Berkshire（B） ×Landrace（L） xYorkshire（Y） had extremely significant difference with Landrace （L） × Yorkshire（Y） and significant difference with Duroc（D） × Landrace（L） × Yorkshire（Y）. The meat color, marbling and cocking percentage and other meat indices were similar with that of Jianhe White Xiang Pig, Nayong Nuogu Pig and Cola Pig. [ Conclusion] Berkshire（B） × Landrace（L） xYorkshire（Y） was more suiTab, for residents in the minority mountain areas, so it was worthy to be popularized.

关键词： Berkshire Carcass traits Meat quality

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外周血miR-27a、miR-146a-5p水平与妊娠期糖尿病患者妊娠结局的关系

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广东医学 2024年第3期45卷 361-365页

作者：汤倩倩张庆王燕李爱萍郑州大学第二附属医院妇产科河南郑州450014

目的探究外周血微小核糖核酸-27a(miR-27a)、微小核糖核酸-146a-5p(miR-146a-5p)水平与妊娠期糖尿病(GDM)患者妊娠结局的相关性。方法回顾性选取2020年1月至2022年12月郑州大学第二附属医院妇产科接收的60例GDM患者当作研究对象,依照妊... 详细信息

目的探究外周血微小核糖核酸-27a(miR-27a)、微小核糖核酸-146a-5p(miR-146a-5p)水平与妊娠期糖尿病(GDM)患者妊娠结局的相关性。方法回顾性选取2020年1月至2022年12月郑州大学第二附属医院妇产科接收的60例GDM患者当作研究对象,依照妊娠结局划分成不良组(n=29)、良好组(n=31),对两组临床资料行单因素分析与logistics多因素回归分析,采用Spearman相关性分析法分析外周血miR-27a、miR-146a-5p水平与妊娠结局的相关性,绘制受试者工作特征曲线(ROC)分析miR-27a、miR-146a-5p对GDM患者妊娠结局的预测价值。结果两组总胆固醇(TC)、三酰甘油(TG)、miR-27a、miR-146a-5p比较差异有统计学意义(P<0.05);logistics多因素回归分析结果显示,miR-27a、miR-146a-5p为GDM患者发生不良妊娠结局的危险因素(P<0.05);Spearman相关性分析结果显示,外周血miR-27a水平与不良妊娠结局发生呈正相关,外周血miR-146a-5p水平与不良妊娠结局发生呈负相关(P<0.05);ROC曲线显示,miR-27a、miR-146a-5p预测GDM患者妊娠结局的曲线下面积(AUC)为0.915、0.902,联合预测的AUC最大,为0.979。结论外周血miR-27a、miR-146a-5p水平与不良妊娠结局发生有关,且联合检测对妊娠结局具有较高预测价值,有助于GDM患者妊娠结局的早期判断。

关键词：微小核糖核酸-27a 微小核糖核酸-146a-5p 妊娠期糖尿病妊娠结局相关性预测价值

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Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

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World Journal of Stem Cells 2024年第4期16卷 444-458页

作者： Cheng-Jin ai ling-Juan Chen li-Xuan Guo Ya-ping Wang Zi-Yi Zhao Department of Laboratory Medicine Hospital of Chengdu University of Traditional Chinese MedicineChengdu 641000Sichuan ProvinceChina Department of Ophthalmology Hospital of Chengdu University of Traditional Chinese MedicineChengdu 641000Sichuan ProvinceChina Central Laboratory Hospital of Chengdu University of Traditional Chinese MedicineChengdu 641000Sichuan ProvinceChina Traditional Chinese Medicine Regulating Metabolic Diseases Key Laboratory of Sichuan Province Chengdu University of Traditional Chinese MedicineChengdu 641000Sichuan ProvinceChina

BACKGROUND Leukemia stem cells(LSCs)are found to be one of the main factors contributing to poor therapeutic effects in acute myeloid leukemia(AML),as they are protected by the bone marrow microenvironment(BMM)against conventional therapies.Gossypol acetic acid(GAA),which is extracted from the seeds of cotton plants,exerts anti-tumor roles in several types of cancer and has been reported to induce apoptosis of LSCs by inhibiting Bcl2.aiM To investigate the exact roles of GAA in regulating LSCs under different microenvironments and the exact mechanism.METHODS In this study,LSCs were magnetically sorted from AML cell lines and the CD34+CD38-population was obtained.The expression of leucine-rich pentatricopeptide repeat-containing protein(LRPPRC)and forkhead box M1(FOXM1)was evaluated in LSCs,and the effects of GAA on malignancies and mitochondrial RESULTS LRPPRC was found to be upregulated,and GAA inhibited cell proliferation by degrading LRPPRC.GAA induced LRPPRC degradation and inhibited the activation of interleukin 6(IL-6)/janus kinase(JAK)1/signal transducer and activator of transcription(STAT)3 signaling,enhancing chemosensitivity in LSCs against conventional chemotherapies,including L-Asparaginase,Dexamethasone,and cytarabine.GAA was also found to downregulate FOXM1 indirectly by regulating LRPPRC.Furthermore,GAA induced reactive oxygen species accumulation,disturbed mitochondrial homeostasis,and caused mitochondrial dysfunction.By inhibiting IL-6/JAK1/STAT3 signaling via degrading LRPPRC,GAA resulted in the elimination of LSCs.Meanwhile,GAA induced oxidative stress and subsequent cell damage by causing mitochondrial damage.CONCLUSION Taken together,the results indicate that GAA might overcome the BMM protective effect and be considered as a novel and effective combination therapy for AML.

关键词： Leukemia stem cells Gossypol acetic acid Reactive oxygen species Mitochondrial dysfunction Interleukin 6/janus kinase 1/signal transducer and activator of transcription 3 signaling

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TRIM4 modulates type I interferon induction and cellular antiviral response by targeting RIG-I for K63-1inked ubiquitination

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Journal of Molecular Cell Biology 2014年第2期8卷 154-163页

作者： Jie Yan Qi li ai-ping Mao Hong-Bing Shu College of life Sciences Wuhan University Wuhan 430072 China

RIG-I 是认出病毒的 RNA 的不同种类的一个枢轴的细胞质的传感器。这识别导致抄写因素 NF-B 和 IRF3，它协作导致打的 activationof 我干扰素(IFN ) 和天生的抗病毒的 response.In 这研究，我们作为 RIG-I-mediated IFN 的一个积极管理... 详细信息

RIG-I 是认出病毒的 RNA 的不同种类的一个枢轴的细胞质的传感器。这识别导致抄写因素 NF-B 和 IRF3，它协作导致打的 activationof 我干扰素(IFN ) 和天生的抗病毒的 response.In 这研究，我们作为 RIG-I-mediated IFN 的一个积极管理者识别了整齐的家庭蛋白质 TRIM4 感应。Overexpressionof TRIM4 加强了 IRF3 和 NF-B 的被触发病毒的激活，以及 IFN- 正式就职，而 TRIM4had 相反的效果击倒。机械学地， TRIM4 与 RIG-I 联系并且为连接 K63 的 polyubiquitination 指向它。我们 TRIM4 是由调停的导致病毒的 IFN 正式就职小径的一个重要管理者的 findingsdemonstrate 为 K63-linkedubiquitination 的 RIG-I。

关键词：病毒诱导抗病毒干扰素泛素化细胞质应答调控转录因子

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