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Changes in the Contents of ATP and Its Related Breakdown Compounds in Various Tissues of Oyster During Frozen Storage

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Journal of Ocean University of China 2007年第4期6卷 407-412页

作者： WANG Qi XUE Changhu li zhaojie FU Xueyan XU Jie XUE Yong College of Food Science and Engineering Ocean University of China Qingdao 266003 P.R. China

The changes in the contents of adenosine 5’-triphosphate (ATP) and its related breakdown compounds were investigated in the adductor muscle, mantle, gill and body trunk of oyster (Crassostrea gigas) during frozen storage at -20℃ and -30℃ and compared with that of the fresh oyster. The investigation was performed using an HPLC system. Different extents of ATP decomposi- tion were found in various tissues frozen at the two temperatures. The K, K’ and A.E.C values were calculated as the chemical fresh- ness indices. Considering the results of sensory evaluation, the A.E.C. value in body trunk at -20℃ and -30℃ could be used as the best freshness index for frozen oyster.

关键词： oyster frozen adenosine 5'-triphosphate (ATP) HPLC freshness index

Identification of Five Sea Cucumber Species Through PCR-RFLP Analysis

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Journal of Ocean University of China 2014年第5期13卷 825-829页

作者： LV Yingchun ZHENG Rong ZUO Tao WANG Yuming li zhaojie XUE Yong XUE Changhu TANG Qingjuan College of Food Science and Engineering Ocean University of China

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene(COI) was used to identifing five sea cucumber species(Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31 I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.

关键词： sea cucumber mtDNA COI gene species identification PCR-RFLP

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从乌海经验看用水预算管理的创新探索

水利发展研究 2024年第3期24卷 69-72页

作者：李肇桀张旺王亦宁刘政平葛静芳水利部发展研究中心北京100038

内蒙古自治区乌海市基于自身水资源条件和现实需求,创新探索用水预算管理模式,建立“零基预算”的用水管理规则,明确用水预算管理各项程序,并将用水预算管理与定额管理、计划用水管理有机结合起来,推进全链条节水,构建水资源需求侧管理... 详细信息

内蒙古自治区乌海市基于自身水资源条件和现实需求,创新探索用水预算管理模式,建立“零基预算”的用水管理规则,明确用水预算管理各项程序,并将用水预算管理与定额管理、计划用水管理有机结合起来,推进全链条节水,构建水资源需求侧管理体系,为提升水资源利用效率提供了制度保障。文章基于对乌海市用水预算管理经验启示的总结,提出对用水预算管理的思考和认识,针对推进全国用水预算管理试点建设提出建议。

关键词：用水预算管理计划用水管理节水管理零基预算

Sequence Analysis and Quantitative Detection of Norwalk-like Viruses in Cultured Oysters of China

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Journal of Ocean University of China 2008年第2期7卷 223-227页

作者： WANG Jun TANG Qingjuan YUE Zhiqin li zhaojie ZHANG Jin XUE Changhu Institute of Food Science and Technology Ocean University of China Qingdao 266003 P. R. China Inspection and Quarantine Bureau of Shandong Qingdao 266003 P. R. China

We isolated 4 Norwalk-like viruses （NLVs） contaminated oysters from 33 Chinese oysters collected from local commercial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase （RdRp） region of NLVs genomes with RT-PCR, the open reading frame 1 （ORF 1） of the RdRp was sequenced and subjected to multiple-sequence alignment. The results showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts （copies） of NLVs in positive patient＇s fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9× 10^8, 1.25× 10^8 and 4.7× 10^1 respectively. The detecting limit of NLVs was 1 × 10^1 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.

关键词： oysters Norwalk-like viruses (NLVs) reverse transcription polymerase chain reaction (RT-PCR) sequence analysis real time quantitative PCR

CHANGES IN liPIDS OF MUSSEL DURING STORAGE

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中国水产科学 1995年第4期 56-63页

作者： Xue Changhu, Wang Yisheng, li zhaojie, Lou Weifeng(Ocaan University of Qingdao 266003)

ＣＨＡＮＧＥＳＩＮＬＩＰＩＤＳＯＦＭＵＳＳＥＬＤＵＲＩＮＧＳＴＯＲＡＧＥＸｕｅＣｈａｎｇｈｕ，ＷａｎｇＹｉｓｈｅｎｇ，ＬｉＺｈａｏｊｉｅ，ＬｏｕＷｅｉｆｅｎｇ（ＯｃａａｎＵｎｉｖｅｒｓｉｔｙｏｆＱｉｎｇｄａｏ２６６０... 详细信息

ＣＨＡＮＧＥＳＩＮＬＩＰＩＤＳＯＦＭＵＳＳＥＬＤＵＲＩＮＧＳＴＯＲＡＧＥＸｕｅＣｈａｎｇｈｕ，ＷａｎｇＹｉｓｈｅｎｇ，ＬｉＺｈａｏｊｉｅ，ＬｏｕＷｅｉｆｅｎｇ（ＯｃａａｎＵｎｉｖｅｒｓｉｔｙｏｆＱｉｎｇｄａｏ２６６００３）ＣＨＡＮＧＥＳＩＮＬＩＰＩＤ...

关键词： Mussel lipid Oxidation

In situ performance and stability tests of large-area flexible polymer solar cells in the 35-km stratospheric environment

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National Science Review 2023年第4期10卷 150-158页

作者： Zihan Xu Guoning Xu Qun Luo Yunfei Han Yu Tang Ying Miao Yongxiang li Jian Qin Jingbo Guo Wusong Zha Chao Gong Kun Lu Jianqi Zhang Zhixiang Wei Rong Cai Yanchu Yang zhaojie li Chang-Qi Ma i-Lab & Printable Electronic Center Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences School of Nano-Tech and Nano-Bionics University of Science and Technology of China Aerospace Information Research Institute Chinese Academy of Sciences University of Chinese Academy of Sciences CAS Key Laboratory of Nanosystem and Hierarchical Fabrication National Center for Nanoscience and Technology

Flexible organic solar cells(FOSCs) are one of the most promising power sources for aerospace aircraft due to their atractive advantages with high power-per-weight ratio and excellent mechanical *** the performance and stability of high-performance FOSCs is essential for the further development of FOSCs for aerospace applications. In this paper, after systematic investigations on the performance of the state-of-the-art high-performance solar cells under thermal cycle and intensive UV irradiation conditions, in situ performance and stability tests of the solar cells in the 35 km stratospheric environment were carried out through a high-altitude balloon uploading. The encapsulated FOSCs with an area of 0.64 cm2gave the highest power density of 15.26 mW/cm2and an efficiency over 11%,corresponding to a power-per-weight ratio of over 3.32 k W/kg. More importantly, the cells showed stable power output during the 3-h continuous flight at 35 km and only 10% performance decay after return to the lab, suggesting promising stability of the FOSCs in the stratospheric environment.

关键词： flexible organic solar cells stratospheric environment 35-km altitude thermal cycle reliability

3D printed grafts with gradient structures for organized vascular regeneration

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International Journal of Extreme Manufacturing 2024年第3期6卷 505-520页

作者： Yuewei Chen Zhongfei Zou Tao Fu Zhuang li zhaojie Zhang Meng Zhu Qing Gao Shaofei Wu Guosheng Fu Yong He Jiayin Fu State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China School of Mechanical Engineering Guizhou UniversityGuiyang 550025People’s Republic of China Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province Department of CardiologySir Run Run Shaw HospitalZhejiang UniversityHangzhou 310016People’s Republic of China School of Mechanical Engineering Guizhou Institute of TechnologyGuiyang 550003People’s Republic of China Department of Oral and Maxillofacial Surgery The Second Affiliated Hospital of Zhejiang University School of MedicineSchool of Stomatologyand Key Laboratory of Oral Biomedical Research of Zhejiang ProvinceHangzhouZhejiang 310006People’s Republic of China

Synthetic vascular grafts suitable for small-diameter arteries(<6 mm) are in great ***,there are still no commercially available small-diameter vascular grafts(SDVGs) in clinical practice due to thrombosis and stenosi... 详细信息

Synthetic vascular grafts suitable for small-diameter arteries(linical practice due to thrombosis and stenosis after in vivo *** designing SDVGs,many studies emphasized reendothelization but ignored the importance of reconstruction of the smooth muscle layer(SML).To facilitate rapid SML regeneration,a high-resolution 3D printing method was used to create a novel bilayer SDVG with structures and mechanical properties mimicking natural *** by the collagen alignment of SML,the inner layer of the grafts had larger pore sizes and high porosity to accelerate the infiltration of cells and their circumferential alignment,which could facilitate SML reconstruction for compliance restoration and spontaneous *** outer layer was designed to induce fibroblast recruitment by low porosity and minor pore size and provide SDVG with sufficient mechanical *** month after implantation,the arteries regenerated by 3D-printed grafts exhibited better pulsatility than electrospun grafts,with a compliance(8.9%) approaching that of natural arteries(11.36%) and significantly higher than that of electrospun ones(1.9%).The 3D-printed vascular demonstrated a three-layer structure more closely resembling natural arteries while electrospun grafts showed incomplete endothelium and immature *** study shows the importance of SML reconstruction during vascular graft regeneration and provides an effective strategy to reconstruct blood vessels through 3D-printed structures rapidly.

关键词： small-diameter vascular graft smooth muscle layer 3D printing endothelialization

Monthly Changes of Glycogen, lipid and Free Amino Acid of Oyster

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Journal of Ocean University of China 2006年第3期5卷 257-262页

作者： ZHANG Zhicui XUEChanghu GAO Xin li zhaojie WANG Qi College of Food Science and Technology Ocean University of China Qingdao 266003 P. R. China

Monthly difference of the chemical composition of oyster cultured along the eastern coast of Shandong Province was analyzed. The components analyzed included glycogen, fatty acid and free amino acid （FAA）. The content of glycogen was high in January and March （2.89 and 2.82 g（100 g） ^-1 on average, respectively） and low in October （2.07g（100g）^- 1 on average）. The low content of neutral lipids in October reflected a relatively poor nutritional value of oyster （1.42 g（ 100 g）^- 1 on average）. The main fatty acids of oyster were palmitic acid （16：0）, oleic acid （18： 1）, eicosapentaenoic acid （EPA, 20： 5（0 3） and docosahexaenoic acid （DHA, 22：6（0 3）. The major FAAs of oyster were Taurine, Glutamicacid, Glycin, Alanine, Arginine and Proline. Taurine was the most abundant FAA with its content ranging from 603 mg（100 g） ^-1 to 1 139 mg（ 100 g） ^-1. The high contents of glycogen, polyunsaturated fatty acid and FAA showed that oyster cultured along the eastern coast of Shandong Province was nutritionally good in January and March.

关键词： oyster glycogen fatty acid free amino acid

Effect of enzymatic fish frame protein hydrolysate on freeze-induced denaturation of myofibrillar protein from bighead carp fish （Aristichthys nobilis ） during frozen storage

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High Technology Letters 2006年第4期12卷 434-440页

作者：薛勇 Xue Changhu Zhang Yongqin li zhaojie Gao Ruichang Gao Xin College of Food Science and Technology Ocean University of China Qingdao 2660031 P.R. China Department of Biologial and Plumnaceutical Engineering Qingdao Univercity of Science and Technology Qingdao 266042 P.R. China

Three kinds offish frame protein hydrolysates （PPH, APH and FPH） were prepared from fish frame of red drum （ Sciaenops ocellatus ） by papain, alkaline proteinase and flavorzyme treatment. The hydrolysates were mainly composed of peptide （83.5 % -84.6% ） and displayed different molecular weight distribution pattern. The protective effects of hydrolysates on the freeze-induced denaturation of myofibrillar protein （Mf） from bighead carp （Aristichthys nobilis） mince during storage at -20℃ for 12 weeks were investigated. The hydrolysate （5 % dried weight/wet weight） reduced the freeze-induced denaturation of Mf as evidenced by the lowered decrease in Ca-ATPase activity and reactive sulfllydryl contents as well as the impeded increase in surface hydrophobicity. Microscopic photographs indicated that the hydrolysates inhibited the growth of ice crystal in fish mince, and then prevented the aggregation of Mf during frozen storage. The protective effects of hydrolysates on freeze-induced denaturation of Mf were influenced by the molecular weight distribution. PPH had strongest cryoprotective ability among three hydrolysates.

关键词： fish frame protein hydrolylsate freeze-induced denaturation Aristichthys nobilis