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Comparison of Near Infrared Spectroscopy Models for Determining Protein and Amylose Contents Between Calibration Samples of Recombinant Inbred lines and Conventional Varieties of Rice

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Agricultural Sciences in China 2007年第8期6卷 941-948页

作者： ZHANG Hong-jiang WU Jin-hong LUO li-jun li Ying YANG Hua YU Xin-qiao WANG Xiao-shan CHEN liang MEI Han-wei Shanghai Agrobiological Gene Center Shanghai 201106 P.R.China

The near infrared spectra of 178 recombinant inbred lines （RILs） from the cross of Ⅱ-32B/Yuezaoxian 6 （YZX6） and 511 varieties in rice were acquired. A total of 80 RILs and 96 cultivars were selected as modeling samples by comparing the spectra similarity primarily. Three partial least square （PLS） regression models were developed, based on the RILs （RIL-model）, the varieties （Var-model） and their mixture （Mix-model）, for protein content （PC） and amylose content （AC）, respectively. Cross validation and outer prediction showed that the models were largely influenced by the range and distribution of modeling samples. The regression model of PC based on the cultivars and the model of AC based on RILs had higher coefficient of determination （r^2 ≥ 0.9） and lower root mean square error of cross validation （RMSECVs）. The disadvantages of RIL samples for PC model and variety samples for AC model were probably caused by the narrow range of variance. Aberrant predictions were obtained for outer sample with PC or AC outside the range or within the distribution gap of modeling samples. The Mix-models gave more reliable prediction as the distribution of RIL and variety modeling samples were complementary to each other.

关键词： Oryza sativa L. protein content amylose content NIR spectroscopy RILs varieties

基于DNA杂交指示剂和银纳米棒阵列芯片构建氯霉素SERS适配体传感器的研究

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光谱学与光谱分析 2023年第11期43卷 3445-3451页

作者：郭贺媛熙李利军冯军林鑫李睿广西糖资源绿色加工重点实验室广西科技大学生物与化学工程学院广西柳州545006 广西科技大学医学部广西柳州545005

氯霉素(CAP)是一种人工合成的抗生素,通过与细菌的核糖体进行结合而抑制蛋白质合成达到抑菌目的。长期摄入残留CAP动源性食品会导致人体发生贫血及白血病,也会使机体产生耐药性严重危害人类健康,许多国家规定不得在畜产品中检出CAP,因此... 详细信息

氯霉素(CAP)是一种人工合成的抗生素,通过与细菌的核糖体进行结合而抑制蛋白质合成达到抑菌目的。长期摄入残留CAP动源性食品会导致人体发生贫血及白血病,也会使机体产生耐药性严重危害人类健康,许多国家规定不得在畜产品中检出CAP,因此,设计一种更快速简便且高特异性的CAP检测方法具有重要意义。以CAP的巯基化适配体(SH-Apt)修饰银纳米棒阵列芯片(芯片)作SERS基底、以DNA杂交指示剂亚甲基蓝(MB)作为拉曼信号分子,利用CAP、CAP适配体互补链(cDNA)与CAP适配体(Apt)竞争结合的关系,构建了一种新型的高特异性CAP-SERS适配体传感器。利用扫描电子显微镜(SEM)、X射线衍射(XRD)及EDS能谱仪对芯片及CAP-SERS适配体传感器进行表征,结果表明芯片表面均匀分布大量银元素且证明CAP-SERS适配体传感器成功制备。在室温条件下对CAP标准样进行检测,传感器相关性能的考察结果表明,在0.001~10 ng·mL^(-1)范围之间,CAP浓度的对数值(logc)与1624 cm^(-1)处的SERS信号强度(I_(SERS))存在良好的线性关系:I_(SERS)=-971log c+1983(R^(2)=0.991)。拉曼增强因子EF=1.01×10^(7),检测限为0.2 pg·mL^(-1)(S/N=3),进一步表明该基底具有良好的拉曼增强效果。应用该传感器分别检测CAP片剂、人血清及猪血清中的CAP,并进行加标试验,结果令人满意,回收率在91.2%~120.5%之间,RSD(n=3)在0.97%~8.1%之间,证明该传感器有良好的准确性。该传感器具有制作简单、灵敏度高、选择性强、重现性和稳定性好以及检测速度快等优点,可望应用于实际样品中CAP的快速定量检测,为检测CAP提供了一种新思路。

关键词：亚甲基蓝适配体表面增强拉曼光谱适配体传感器氯霉素

Elevated plasma lipoprotein-associated phospholipase A2 activity is associated with plaque rupture in patients with coronary artery disease

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Chinese Medical Journal 2011年第16期124卷 2469-2473页

作者： liU Chuan-fen QIN li REN Jing-yi CHEN Hong WANG Wei-min liU Jian SONG jun-xian li li-jun Department of Cardiology Peking University People's Hospital Beijing 100044China Department of Laboratory Medicine Peking University People's Hospital Beijing 100044China

Background lipoprotein-associated phospholipase A2 （Lp-PLA2） has recently been shown to be positively related to coronary events in patients with coronary artery disease （CAD）. However, direct evidence about the relationship between circulation Lp-PLA2 activity and vulnerable plaque in patients with CAD remains lacking. Methods Plasma Lp-PLA2 activity was determined in 146 consecutive patients with CAD who underwent clinically-indicated coronary angiography and preinterventional intravascular ultrasound （IVUS）. Results Eighty-three patients were included in the final analysis after the initial screening. Sixty （72.3%） were acute coronary syndrome （ACS） patients and 23 （27.7%） were stable angina pectoris （SAP） patients. Plaque rupture occurred in 39 （47.0%） patients, and 34 （87.2%） were from ACS patients and 5 （12.8%） from SAP patients. There were no significant differences in clinical and angiographic characteristics between patients with plaque rupture and those without plaque rupture, except for smoking, high-sensitive C-reactive protein （hs-CRP） level and Lp-PLA2 activity （all P 〈0.05）. IVUS measurement uncovered that patients with plaque rupture had more frequent positive remodeling （74.4% vs. 43.2%, P=0.004）, soft plaques （64.1% vs. 36.4%, P=-0.012） and higher remodeling index （1.13~0.16 vs. 0.99＋_0.11, P=0.041） as compared with those without plaque rupture. Multivariate Logistic regression analysis showed that plasma Lp-PLA2 activity was independently associated with plaque rupture after adjusting for smoking, positive remodeling and soft plaque （Model 1： odds ratio （OR） 1.13, 95% confidence interval （CO ： 1.06-1.20） or adjusting for smoking, hs-CRP level, positive remodeling and soft plaque （Model 2： OR 1.11,95%C1： 1.04-1.19）. Conclusions Plasma Lp-PLA2 activity is associated with plaque rupture in patients with CAD, independently of traditional CAD risk factors, hs-CRP level and IVUS parameters. Lp-PLA2 may be a risk marker for vulnerable plaques.

关键词： lipoprotein-associated phospholipase A2 plaque intravascular ultrasound inflammation coronary artery disease

Value of T cell receptor gamma alternate reading frame protein and keratin 5 in endometrial carcinoma

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Chinese Medical Journal 2013年第22期126卷 4260-4264页

作者： ZHAO li-jun li Xiao-ping QI Wen-juan WANG Jian-liu WEI li-hui Department of Obstetrics & Gynecology Peking University People's Hospital Beijing 100044 China

Background Tumors with different gene expression develop and progress in different ways. To deepen our understanding of the progression in endometrial cancer, and provide a useful tool for accurate diagnosis and prognosis assessment, we identified the new molecular prognostic markers in endometrial carcinoma and analyzed the relationship of them with clinical and pathological features of endometrial carcinoma. Methods Ninety-four cases of endometrial endometrioid adenocarcinoma with complete data from the Peking University People＇s Hospital from 2000 to 2008 and 40 cases of normal endometrium were enrolled. Among these, 30 endometrial endometrioid adenocarcinoma samples of different International Federation of Gynecology and Obstetrics （FIGO） stage were selected for further Agilent genome-wide microarray analysis. Significance analysis of microarrays （SAM） was used to identify genes that are significantly associated with tumor progress. Immunohistochemistry was utilized to identify the genes of interest in endometrial carcinoma and normal endometrium. The relationship between the genes and the age, clinical stage, histological grade, myometrium invaded depth, lymph node metastasis status, and the expression of ER, PR, P53, and PTEN were analyzed by X2 test. Results Analysis between FIGO 1988 stage I and stage III identified a 362-gene ＂progress signature＂; 171 downregulated and 191 up-regulated genes. Among the alterative genes, TARP （T cell receptor gamma alternate reading frame protein） and KRT5 （keratin 5） decreased 3.57 fold and 5.8 fold in FIGO stage III patients. The expression of TARP in endometrial carcinoma increased compared to normal endometrium, while that of KRT5 decreased （P〈0.05）. The expression of TARP and KRT5 decreased when stage, histological grading, myometrium invaded depth increased （P〈0.05）. In the cases with lymph node metastasis, the expression of TARP decreased, while the expression of KRT5 did not differ （both P〈0.05） both. The expression

关键词： endometrial cancer T cell receptor gamma alternate reading frame protein keratin 5

Study on Massive Open Online Course and College English Teaching Reform

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Sino-US English Teaching 2015年第9期12卷 721-725页

作者： XU li-mei TONG li-jun Changchun University Changchun China

English is the universal language. With the development of economic and cultural globalization, the demand for English has gradually increased. Traditional college English teaching cannot meet real requirement, so college English teaching reform is imperative. With the development of online platform, the Internet has brought a great impetus to the curriculum reform. The author thinks that ＂MOOC＂ and College English Teaching Reform have a good point of combining, but there is still a problem remaining to be solved： How to make good use of＂MOOC＂ for College English Teaching Reform service has a lot study space in the future.

关键词： MOOC College English Teaching Reform combination

Challenge and Revelation Massive Open Online Course Brings to the British and American literature Teaching

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Sino-US English Teaching 2015年第10期12卷 806-810页

作者： XU li-mei TONG li-jun Changchun University Changchun China

Massive Open Online Course （MOOC） has many characteristics, such as mass, openness, and online learning, which can effectively make up for the problem ＆shortage of teachers in the college English teaching. But, there is also a challenge to introduce MOOC to the college English teaching. This paper analyzes the challenge and revelation MOOC brings to college English teaching combined with the characteristics of MOOC, and points out that the role of teachers, students, and managers should be changed under the background of MOOC.

关键词： MOOC college English teaching challenge and revelation

ACTIVE PIEZOELECTRIC-STRUCTURE ACOUSTIC CONTROL OF ENCLOSED-CAVITY

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ACTIVE PIEZOELECTRIC-STRUCTURE ACOUSTIC CONTROL OF ENCLOSED-...

2012全国压电和声波理论及器件技术研讨会

作者： li-jun li Shi-jie ZHENG StateKeyLaboratoryofMechanicsandControlofMechanicalStructuresCollegeofAerospaceEngineering NanjingUniversityofAeronautics&Astronautics

Piezoelectric sensor and actuator has been highly concerned in active control with its perfect *** this paper,a refined hybrid enhanced assumed strain piezoelectric shell element and acousti

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渗碳齿轮齿根部的硬化层深度分析

金属热处理 2011年第4期36卷 95-97页

作者：黎丽君四川优机实业股份有限公司四川成都610041

渗碳齿轮的硬化层深度通常按现有标准检测齿轮节圆部位,对齿根处的硬化层深度没有提出要求。但在实际使用中,齿根承受的交变应力最大,齿根的硬化层深度对齿轮的弯曲疲劳寿命影响最大,目前对齿轮齿根渗碳层研究的数据不多。在一些国外提... 详细信息

渗碳齿轮的硬化层深度通常按现有标准检测齿轮节圆部位,对齿根处的硬化层深度没有提出要求。但在实际使用中,齿根承受的交变应力最大,齿根的硬化层深度对齿轮的弯曲疲劳寿命影响最大,目前对齿轮齿根渗碳层研究的数据不多。在一些国外提供的加工图纸中,要求渗碳齿轮节圆。

关键词：硬化层深度渗碳齿轮深度分析根部轮齿弯曲疲劳寿命交变应力齿根

Interventions to improve chronic cyclosporine A nephrotoxicity through inhibiting renal cell apoptosis： a systematic review

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Chinese Medical Journal 2013年第19期126卷 3767-3774页

作者： XIAO Zheng li Cheng-wen SHAN Juan LUO Lei FENG li LU jun li Sheng-fu LONG Dan li You-ping Key Laboratory of Transplant Engineering and Immunology of Ministry Health of China West China Hospital Sichuan University ChengduSichuan 610041 China

Objective To reveal interventions for chronic cyclosporine A nephrotoxicity （CCN） and provide new targets for further studies,we analyzed all relevant studies about interventions in renal cell *** sources We collected all relevant studies about interventions for cyclosporine A （CsA）-induced renal cell apoptosis in Medline （1966 to July 2010）,Embase （1980 to July 2010） and ISI （1986 to July 2010）,evaluated their quality,extracted data following PICOS principles and synthesized the *** selection We included all relevant studies about interventions in CsA-induced renal cell apoptosis no limitation of research design and language） and excluded the duplicated articles,meeting abstracts and reviews without specific *** There were three kinds of intervention,include anti-oxidant （sulfated polysaccharides,tea polyphenols,apigenin,curcumin,spirulina,etc）,biologics （recombinant human erythropoietin （rhEPO）,a murine pan-specific transforming growth factor （TGF）-beta-neutralizing monoclonal antibody1D11,cartilage oligomeric matrix protein （COMP）-angiopoietin-1 and hepatocyte growth factor （HGF） gene）,and other drugs （spironolactone,rosiglitazone,pirfenidone and colchicine）.These interventions significantly improved the CCN,renal cell apoptosis and renal dysfunction through intervening in four apoptotic pathways in animals or protected renal cells from apoptosis induced by CsA and increased cell survival through respectively four pathways in *** There are three group interventions for *** anti-oxidant drugs can significantly improve CCN,renal cell apoptosis and renal *** drugs can improve CCN through intervening in Fas/Fas ligand or mitochondrial pathway with sufficient *** Ⅱ,nitric oxide （NO） and endoplasmic reticulum （ER） pathways will be new targets for CCN.

关键词： cyclosporine A interventions renal cells apoptosis chronic cyclosporine A nephrotoxicity