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基于相位梯度表面的缝隙阵列天线宽带RCS减缩

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电波科学学报 2018年第3期33卷 330-336页

作者：刘英李娜贾永涛龚书喜西安电子科技大学信息感知技术协同创新中心天线与微波技术重点实验室西安710071

提出了一种基于耶路撒冷十字单元的相位梯度表面,并将该表面加载到缝隙阵列天线表面.通过利用该表面将空间波(propagating wave,PW)转化为表面波(surface wave,SW)及奇异反射特性,天线可以在很宽的频带内实现显著的雷达散射截面(radar c... 详细信息

提出了一种基于耶路撒冷十字单元的相位梯度表面,并将该表面加载到缝隙阵列天线表面.通过利用该表面将空间波(propagating wave,PW)转化为表面波(surface wave,SW)及奇异反射特性,天线可以在很宽的频带内实现显著的雷达散射截面(radar cross section reduction,RCS)减缩.与参考天线相比,设计天线在TE和TM两种极化波垂直和斜入射状态下均在6~18GHz频带范围内实现了单站RCS减缩,并且在9.5GHz处的最大减缩量达到20dB.与传统的天线RCS减缩技术相比,该方法可以在保证天线原本辐射性能的基础上同时实现天线带内和带外的RCS减缩.

关键词：相位梯度表面广义斯涅尔定律缝隙阵列天线雷达散射截面宽带

Genome-wide identification and expression profiling analysis of naC transcription factor family in oilseed rape(Brassica napus L.)

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Genome-wide identification and expression profiling analysis...

A semisynthetic cabazitaxel analogue, C1 overcomes P-gp-meditaed multidrug resistance in non-small-cell lung cancer

中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会

作者： ZHANG Chao MA Jin-qi TANG Xin-yu YANG Bo ZHANG li-yuan liU Pu liU Jing-sen LU Kun li jia-na Chongqing Rapeseed Engineering Research Center College of Agronomy and BiotechnologySouthwest University Academy of Agricultural Sciences Southwest University

naC(naM,ATAF,CUC) is a largely plant-specific transcription factor family involved in the regulation of plant growth,development and defense to abiotic and biotic *** genes have been comprehensively characterized in many plants,but not in rapeseed(Brassica napus).In the current study,319 naCs were identified in Brassica napus genome,and were divided into 9 groups(Groups A-1) based on phylogenetic analysis of their deduced protein *** addition,24 of 319 BnnaCs,belonging to Group A and C,were targets of micro RnamiR164,indicating miR164 mediated posttranscriptional regulation of these BnnaC *** extracted fragments per kilobase million(FPKM) for each gene from Rna-sequencing data obtained from 14 different *** Zhongshuang 11 tissues at various developmental *** genes exhibited different expression patterns in various tissues,revealing that they are differentially ***,weighted gene co-expression network analysis(WGCna) was used to toelaborate dynamic expression levels of BnnaCs inoculated by Sclerotinia sclerotiorum and Leptosphaeria maculans,respectively,which suggested that 7 of 24 miR 164 target BnnaCs played crucial roles on the defense to *** together,our results provided a valuable foundation for functional dissection of different BnnaC homologs in *** and revealed importance of BnnaCs interacted with miRl64 to pathogen infection response.

关键词： naC gene family phylogenetic analysis expression pattern WGCna Brassica napus L.

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中国药理学与毒理学杂志 2019年第10期 757-757页

作者： jia li-na TANG Xing YANG Jing-yu WU Chun-fu Department of Pharmacology Shenyang Pharmaceutical University Benxi Institute of Pharmaceutical Research Shenyang Pharmaceutical University

OBJECTIVE To investigate the effect of C1 against P-glycoprotein（P-gp）-meditaed multidrug resistance（MDR） in non-small-cell lung cancer cells. METHODS Human non-small-cell lung cancer（NSCLC） cell lines A549, H1299, H460, normal lung cell lines MRC5,taxol-resistant daughter line A549/TR and cisplatin-resistant daughter A549/CR were used to achieve this objective. Cell proliferation was analyzed by cytotoxicity assay,P-gp ATPase activity in vitro was detected by P-gp-Glo?Assay System, P-gp activity in vivo was determined by FACS analyses of intracellular accumulation of Rh123 in A549/TR and A549/CR cells, anti-tumor effect of C1 and taxol were evaluated by parental and drug-resistant cell xenograft of nude mice. RESULTS Cytotoxicity assay results showed that C1 potently inhibits cell proliferation in NSCLC cell lines but not in normal lung cells. However,C1 was more efficacious than taxol in the A549/TR-and A549/CR-treated cells that overproduced P-gp. The drugefflux function of P-gp depends on the ATP hydrolysis that reflects ATPase activity. Taxol, the most widely used taxoid in NSCLC treatment, could inhibit P-gp ATPase activity at lower concentrations, but stimulated it at higher concentrations. In contrast, C1 could significantly inhibit P-gp ATPase activity in a concentration-dependent *** the drug-resistant cells were treated in the presence of C1 at 0.1, 0.2 and 0.4 μmol·L-1, the intracellular accumulation of rhodamine 123 was higher than that in A549/TR and A549/CR treated with taxol at the same concentrations, respectively. These results indicated that C1 can reduce drug efflux through inhibiting the transmembrane pumping function of P-gp but not decreasing the expression of P-gp protein. We next compared the efficacy of TM2 and taxol in vivo using A549, A549-Taxol,and A549-CDDP xenograft models. In parental and drugresistant cell xenograft, C1 could significantly inhibit tumor growth in a dose-dependent manner. The TGI for the group receiving doceta

关键词： cabazitaxel analogue overcome MDR NSCLC

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油菜F_2无性系群体稳定性的SSR检测

中国油料作物学报 2005年第1期27卷 10-13页

作者：凌英华李加纳殷家明西南农业大学农学与生命科学学院重庆北碚400716

为了研究F2 无性系群体作为可重复作图群体的效率,将油菜F2 种子进行组织培养,以试管苗茎尖、子叶和下胚轴为材料,分别进行快繁培养,获得F2 无性系群体。以各F2 试管苗子叶Dna(原供体)作为模板,用100对覆盖油菜全基因组的SSR引物进行扩... 详细信息

为了研究F2 无性系群体作为可重复作图群体的效率,将油菜F2 种子进行组织培养,以试管苗茎尖、子叶和下胚轴为材料,分别进行快繁培养,获得F2 无性系群体。以各F2 试管苗子叶Dna(原供体)作为模板,用100对覆盖油菜全基因组的SSR引物进行扩增,从中选取20对扩增多态性高且带纹较强的引物,对03H109F2 无性系群体进行SSR扩增反应。比较再生植株和原供体间的SSR标记带,结果表明茎尖再生后代的变异率为0. 0059%,接近自然变异率,而子叶和下胚轴再生后代的变异率分别为0. 5271%和0. 2095%,因此认为以茎尖再生所构建的F2无性系群体可以作为可重复作图群体。

关键词：油菜 F2无性系 SSR 稳定性

利用甘蓝型油菜高密度SNP遗传图谱定位油酸、亚麻酸及芥酸含量QTL位点

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中国农业科学 2014年第1期47卷 24-32页

作者：刘列钊李加纳西南大学农学与生物科技学院

【目的】菜籽油包括多种脂肪酸组分,提高油酸(C18:1)含量,降低亚麻酸(C18:2)和芥酸(C22:1)含量是油菜育种改良和遗传研究的重要目标。本研究利用刚开发的油菜60K芯片构建的高世代重组自交系群体遗传连锁图谱,对3个不同环境中影响甘蓝型... 详细信息

【目的】菜籽油包括多种脂肪酸组分,提高油酸(C18:1)含量,降低亚麻酸(C18:2)和芥酸(C22:1)含量是油菜育种改良和遗传研究的重要目标。本研究利用刚开发的油菜60K芯片构建的高世代重组自交系群体遗传连锁图谱,对3个不同环境中影响甘蓝型油菜品质的油酸、亚麻酸及芥酸含量进行QTL定位分析,研究结果可对脂肪酸组分QTL位点在不同的群体之间准确比较分析。【方法】以高芥酸亲本GH06为母本和低芥酸亲本P174为父本构建高世代重组自交系,分别于2008年在德国吉森、德国霍亨里特及2009年德国吉森3个不同的环境中设置田间试验,收获自交种子,采用近红外分析方法 3次重复对种子的脂肪酸组分进行分析。利用油菜60K芯片对重组自交系群体进行基因型分析,Dna样品预处理及芯片处理严格按照Illumina Inc公司Infinium HD Assay Ultra操作说明进行。取最小阈值LOD 5.0利用MSTmap软件构建遗传图谱。QTL定位所用的遗传图谱包括2 756个SNP位点,覆盖甘蓝型油菜基因组1 832.4 cM。利用Windows QTL Cartographer复合区间作图法对油酸、亚麻酸及芥酸含量进行QTL定位。【结果】在3个环境中,油酸和芥酸含量均表现为极显著负相关,相关系数均达到-0.95,且表现为主基因控制的性状,芥酸和亚麻酸表现负相关,油酸与亚麻酸表现正相关。3个性状在3个环境中共检测到14个QTL,在A08和C03上都检测到油酸和芥酸含量重叠的主效QTL位点。在3个环境中,油酸主效QTL位点解释表型变异19%—31%,芥酸主效QTL位点解释表型变异19%—34%,两者表现加性效应相反。A08和C03染色体上的芥酸主效QTL位点加性效应在3个环境中为7.6到9.6,加性效应来自低油酸、高芥酸亲本GH06。亚麻酸属于典型的数量性状,受环境影响较大,在3个环境中检测到不同的微效QTL位点,解释表型变异3%—12%。遗传图谱与物理图谱比较分析发现,脂肪酸去饱和酶FAD2基因位于亚麻酸QTL qA05C18:3的置信区间,而脂肪酸延长酶FAE1基因位于芥酸QTL qA08C22:1的置信区间。【结论】利用该套油菜60K芯片准确定位了油酸、亚麻酸及芥酸QTL位点,位于A08和C03染色体上的芥酸主效QTL位点同时也是油酸的主效QTL位点,该研究结果有利于不同群体在使用该套SNP芯片分析及对脂肪酸组分定位后准确比较分析。

关键词：甘蓝型油菜单核苷酸多态性脂肪酸 QTL

Transcriptome analysis on dominant GMS homozygous two-type line D3AB in Brassica napus L. by Rna-Seq

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Transcriptome analysis on dominant GMS homozygous two-type l...

中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会

作者： jia Le-dong XU Dai-xiang MA Guo-qiang DUAN Mou-zheng QIAO Cai-lin ZHOU Xin-tong QU Cun-min WANG Rui li jia-na Chongqing Rapeseed Engineering Technology Research Center College of Agronomy and BiotechnologySouthwest University Academy of Agricultural Sciences Southwest University

Genie male sterility(GMS) has been widely used in Brassica napus breeding because of its stable and thorough sterility and wide distribution of restorer lines.D3 AB is a near isogenic line(NIL) controlled by a single dominant gene,but the specific molecular mechanisms leading to sterility is still *** this study,the transcriptome data of male sterile D3 A and fertile D3 B at different developmental stages of buds was compared,and some related genes causing abortion were identified.A total of 2 210 differentially expressed genes(DEGs) were detected,of which 374 genes were differentially expressed between young buds(≤1 mm) and middle buds(1-2 mm) in D3 A,and 1 458 in D3 B ***,679 differentially expressed genes were detected in young buds of D3 A and D3 B,and 1 423 in the middle buds ***,based on the results of cytological analysis,90 DEGs were specifically expressed in young buds,23 genes of them were up-regulated and67 were down-regulated,*** the gene expression profile database of Arabidopsis thaliana,10 DEGs were specifically expressed in young flower buds or pollen grains,and the expression patterns of 9 DEGs were consistent with transcriptome sequencing by *** results lay the foundation for further study on the abortive mechanism of D3 AB in Brassica napus.

关键词： Brassica napus dominant GMS two-type line Rna-seq

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激素对油菜子叶再生及转基因瞬间表达的影响

西南师范大学学报（自然科学版） 2007年第5期32卷 50-55页

作者：谢伶俐殷家明李加纳柴友荣许本波林呐重庆市油菜工程技术研究中心西南大学农学与生物科技学院重庆400716 长江大学生命科学学院

以甘蓝型油菜"湘油15"子叶为外植体,以MS为基本培养基,采用正交试验设计,研究了预培养基2,4-D和6-BA浓度、分化/共培养基naA和6-BA浓度对子叶芽再生频率和转GUS基因瞬间表达率的影响.结果表明,激素对芽再生频率和转基因瞬间表达率都有... 详细信息

以甘蓝型油菜"湘油15"子叶为外植体,以MS为基本培养基,采用正交试验设计,研究了预培养基2,4-D和6-BA浓度、分化/共培养基naA和6-BA浓度对子叶芽再生频率和转GUS基因瞬间表达率的影响.结果表明,激素对芽再生频率和转基因瞬间表达率都有重要影响,但激素对芽再生和转基因瞬间表达的影响效应具有不一致性.激素对芽分化的影响程度从大到小依次为再生培养基naA、预培养基6-BA、预培养基2,4-D、再生培养基6-BA,推导的对于芽再生的最佳激素组合方案为预培养基2,4-D 1.0 mg/L和6-BA1.0 mg/L、再生分化培养基naA0.2 mg/L和6-BA3.0 mg/L;激素对GUS基因瞬间表达的影响程度从大到小依次为共培养基naA、共培养基6-BA、预培养基6-BA、预培养基2,4-D,推导的对于瞬间表达的最佳激素组合方案为预培养基2,4-D 0mg/L和6-BA0.2 mg/L,共培养基naA0.05 mg/L和6-BA3.0 mg/L.推导的对于芽再生频率×GUS基因瞬间表达率的最佳激素组合方案为预培养基2,4-D 1.0 mg/L和6-BA1.0 mg/L,共/分化培养基naA0.05 mg/L和6-BA3.0 mg/L.

关键词：甘蓝型油菜子叶激素再生瞬间表达

Recent Strategies and Advances in Hydrogel‑Based Delivery Platforms for Bone Regeneration

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nano-Micro Letters 2025年第3期17卷 389-439页

作者： Xiao Wang jia Zeng Donglin Gan Kun ling Mingfang He jianshu li Yongping Lu Scientific and Technological Innovation Center for Biomedical Materials and Clinical Research Guangyuan Key Laboratory of Multifunctional Medical HydrogelGuangyuan Central HospitalGuangyuan 628000People’s Republic of China jiangsu Collaborative Innovation Center of Biomedical Functional Materials Jiangsu Key Laboratory of Bio‑Functional MaterialsSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023People’s Republic of China College of Polymer Science and Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065People’s Republic of China

Bioactive molecules have shown great promise for effectively regulating various bone formation processes,rendering them attractive therapeutics for bone ***,the widespread application of bioactive molecules is limited by their low accumulation and short half-lives in *** have emerged as ideal carriers to address these challenges,offering the potential to prolong retention times at lesion sites,extend half-lives in vivo and mitigate side effects,avoid burst release,and promote adsorption under physiological *** review systematically summarizes the recent advances in the development of bioactive molecule-loaded hydrogels for bone regeneration,encompassing applications in cranial defect repair,femoral defect repair,periodontal bone regeneration,and bone regeneration with underlying ***,this review discusses the current strategies aimed at improving the release profiles of bioactive molecules through stimuli-responsive delivery,carrier-assisted delivery,and sequential ***,this review elucidates the existing challenges and future directions of hydrogel encapsulated bioactive molecules in the field of bone regeneration.

关键词： Hydrogel Bone regeneration Bioactive molecules Drug delivery nano-/microscale carriers

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重庆皱皮木瓜挥发性成分的GC-MS分析

中药材 2012年第5期35卷 728-733页

作者：刘世尧白志川李加纳西南大学园艺园林学院/南方山地园艺学教育部重点实验室重庆北碚400715 西南大学农学与生物科技学院重庆北碚400715

目的:分析测定重庆产皱皮木瓜的挥发性成分。方法:采用水蒸气蒸馏法提取皱皮木瓜鲜果中的香气成分,并用气相色谱-质谱联用仪(GC-MS)进行香气成分的分离鉴定。结果:在重庆产皱皮木瓜鲜果中分离鉴定出芳香性成分106种,以酯类、醇类、羧酸... 详细信息

目的:分析测定重庆产皱皮木瓜的挥发性成分。方法:采用水蒸气蒸馏法提取皱皮木瓜鲜果中的香气成分,并用气相色谱-质谱联用仪(GC-MS)进行香气成分的分离鉴定。结果:在重庆产皱皮木瓜鲜果中分离鉴定出芳香性成分106种,以酯类、醇类、羧酸类、烷烃和烯烃类、酮类化合物为主。结论:GC-MS分析法明确了重庆产皱皮木瓜鲜果的挥发性香气成分组成,为重庆产皱皮木瓜果实开发应用提供了基础数据。

关键词：皱皮木瓜挥发油水蒸气蒸馏法气相色谱-质谱