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Analysis of the lignin Contents and Related Enzymes Activities in Seed Coat Between Black-Seeded and Yellow-Seeded Rapes (Brassica napus L.)

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Agricultural Sciences in China 2005年第12期4卷 890-897页

作者： RAN Xiu-zhi liANG Ying li jia-na College of Agronomy and life Science Southwest Agricultural University Chongqing 400716 P.R.China

One pair of near isonegic yellow/black seeded rape （Brassica napus L） were used as experimental materials to study the changes of lignin contents and enzymes activities of 4-coumarate： CoA ligase （4CL）, Cinnamyl alcohol dehydrogenase （CAD） and ferulate 5-hydroxylase （F5H） in seedcoats during the stage of seed development. The variation analysis showed that the changes of lignin contents and enzyme activities of 4CL, CAD and F5H in seed coat had significant differences between black- and yellow-seeded rapes, and also between different development stages. The correlation analysis demonstrated that the lignin contents were positively correlative to the activities of all three enzymes investigated in the study, and the interactions between them in the seed coat of the two lines. For yellow-seeded rape, the correlation coefficient （0.7262018） of lignin content and the interaction between 4CL and F5H was significant, the lignin contents were highly positively correlative to the activities of CAD and F5H, the interaction between 4CL and CAD, and the interaction between CAD and F5H. For the black-seeded rape, only the lignin content was highly positively correlative to the activity of F5H （the correlation coefficient was 0.772949）, the other correlation coefficients, i. e. lignin contents to 4CL, CAD activities, the interactions between the three enzymes were not significant although all the correlation coefficients were above 0.5000. The results suggested that 4CL, CAD and F5H regulated the biosynthesis of lignin in seedcoat of rapes, leading to the lignin contents in the seedcoats of the yellow-seeded rape much lower than that of the black-seeded line, and affecting the thickness of the developing seedcoats in rapes. Therefore, it was likely to change the seedcoat ratio by overexpressing or suppressing the activities of one of the enzymes, both of them or all of them.

关键词： Brassica napus L.0 lignin contents0 4CL CAD F5H

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Biotechnology ofα-linolenic acid in oilseed rape(Brassica napus)using FAD2 and FAD3 from chia(Salvia hispanica)

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Journal of Integrative Agriculture 2023年第12期22卷 3810-3815页

作者： XUE Yu-fei Inkabanga Tseke ALAIN YIN Neng-wen jiaNG jia-yi ZHAO Yan-ping LU Kun li jia-na DING Yan-song ZHANG Shi-qing CHAI You-rong Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City and Southwest University/Chongqing Key Laboratory of Crop Quality Improvement/College of Agronomy and Biotechnology Southwest UniversityChongqing 400715P.R.China Engineering Research Center of South Upland Agriculture Ministry of Education/Academy of Agricultural SciencesSouthwest UniversityChongqing 400715P.R.China Faculty of Agricultural Sciences National Pedagogic University(UPN)Kinshasa 8815D.R.Congo

α-linolenic acid(ALA,18:3Δ9,12,15)is an essential fatty acid for humans since it is the precursor for the biosynthesis of omega-3 long-chain polyunsaturated fatty acids(LC-PUFA).Modern people generally suffer from deficiency of ALA because most staple food oils are low or lack ALA *** enrichment of ALA in staple oil crops is a promising ***(Salvia hispanica)has the highest ALA content in its seed oil among known oil *** this study,the FAD2 and FAD3 genes from chia were engineered into a staple oil crop,oilseed rape(Brassica napus),via Agrobaterium tumefaciens-mediated transformation of their LP4-2A fusion gene construct driven by the seed-specific promoter P_(napA).In seeds of T0,T1,and T2 lines,the average ALA contents were 20.86,23.54,and 24.92%,respectively,which were 2.21,2.68,and 3.03 folds of the non-transformed controls(9.42,8.78,and 8.22%),*** highest seed ALA levels of T0,T1,and T2 plants were 38.41,35.98,and 39.19%respectively,which were 4.10-4.77 folds of the respective ***-pathway enzyme genes(BnaCCD,BnFATA,BnSAD,BnSCD,BnDGAT1,BnDGAT2,and BnDGAT3)and positive regulatory genes(BnWRI1,BnLEC1,BnL1L,BnLEC2,BnaBI3,BnbZIP67,and BnMYB96)were all significantly *** contrast,BnTT1,BnTT2,BnTT8,BnTT16,BnTTG1,and BnTTG2,encoding negative oil accumulation regulators but positive secondary metabolism regulators,were all significantly *** means the foreign ShFAD2-ShFAD3 fusion gene,directly and indirectly,remodeled both positive and negative loci of the whole FA-related network in transgenic *** seeds.

关键词： biotechnology α-linolenic acid oilseed rape(Brassica napus) FAD2 FAD3 chia(Salvia hispanica)

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Effect of holding pressure on density and cooling rate of cast Al-Si alloy during additive pressure casting

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China Foundry 2019年第6期16卷 363-370页

作者： Zhen Fan Wei Song Hua-rui Zhang Bao-sheng Zhou Xiao-yan Wu li-na jia Hu Zhang School of Materials Science and Engineering Beihang UniversityBeijing 100191China Qingdao Research Institute of Beihang Universtiy Qingdao 266000China Ningbo Institute of Technology Beihang UniversityNingbo 315832China

The cast Al-Si alloy was fabricated using the Additive Pressure Casting(APC)*** effects of holding pressure from 50 to 400 k Pa on the density,cooling rate,and mechanical properties of the alloy,and the corresponding mechanism were *** results indicate that the application of high holding pressure(300 k Pa)enhances the feeding ability of the alloy,leading to an increase of the ***,the cooling rate of the alloy is increased by 100%.In addition,the tensile testing results show that the increase of holding pressure from 50 to 300 k Pa improves the tensile strength and elongation of the alloy by 6.2%and 81.3%,***,excessive holding pressure(400 k Pa)might lower the density and cooling rate of the alloy due to the feeding channels being blocked.

关键词： holding pressure cast Al-Si alloy density cooling rate mechanical properties additive pressure casting

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Seed oil of Brucea javanica induces apoptosis through the PI3K/Akt signaling pathway in acute lymphocytic leukemia Jurkat cells

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Chinese Journal of natural Medicines 2021年第8期19卷 608-620页

作者： ZHANG Hong YIN Shi-liang WANG li-Hui jia li-na SU Guang-Yue liU Xiao-Qing ZHOU Fan BRESliN Peter MENG Ran li Qi-Yi YANG Jing-Yu WU Chun-Fu Department of Pharmacology Shenyang Pharmaceutical UniversityShenyang 110016China Department of Pharmacology Shenyang Medical CollegeShenyang 110034China Faculty of Functional Food And Wine Shenyang Pharmaceutical UniversityShenyang 110016China Department of Technology Industry of Shenyang Pharmaceutical University of Lei Yun Shang Pharmaceutical Co.Ltd.Benxi 117004China Department of Hematology General Hospital of Northern Theater CommandShenyang 11016China Department of Molecular/Cellular Physiology Loyola University ChicagoChicago 60153USA School of Pharmacy Shenyang Pharmaceutical UniversityShenyang 110016China

Brucea javanica oil emulsion(BJOE)has been used to treat tumor in China for more than 40 ***,its components and effectiveness in the treatment of acute lymphocytic leukemia(ALL)and its mechanism of anti-cancer activity remain *** the current study,high-performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)was used to analyze the components of ***,the anti-leukemia effects of BJOE were examined both in vitro and in vivo using ALL Jurkat cells and the p388 mouse leukemia transplant model,*** primary ALL leukemia cells were also used to confirm the antileukemia effects of *** apoptotic-related results indicated that BJOE induced apoptosis in Jurkat cells and were suggestive of intrinsic apoptotic ***,BJOE inhibited Akt(protein kinase B)activation and upregulated its downstream targets p53 and Fox O1(forkhead box gene,group O-1)to initiate *** activation of GSK3βwas also *** findings demonstrate that BJOE has anti-leukemia effects on ALL cells and can induce apoptosis in Jurkat cells through the phosphoinositide3-kinase(PI3 K)/Akt signaling pathway.

关键词： Apoptosis Cancer Leukemia Brucea javanica oil emulsion

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Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP

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Signal Transduction and Targeted Therapy 2023年第3期8卷 1269-1281页

作者： Chao Xiong li-na jia Wei-Xi Xiong Xin-Tong Wu liu-lin Xiong Ting-Hua Wang Dong Zhou Zhen Hong Zheng liu lin Tang Department of Neurology State Key Lab of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center for Biotherapy610041 ChengduSichuanChina Institute of Brain Science and Brain-inspired Technology of West China Hospital Sichuan UniversityChengduChina Institute of Neurological Disease State Key Lab of BiotherapyWest China HospitalSichuan University610041 ChengduSichuanChina School of life and Health Kobilka Institute of Innovative Drug Discoverythe Chinese University of Hong Kong(Shenzhen)ShenzhenChina

Dysfunctions of ATP-binding cassette,subfamily D,member 1(ABCD1)cause X-linked adrenoleukodystrophy,a rare neurodegenerative disease that affects all human *** in the peroxisome membrane,ABCD1 plays a role in the translocation of very long-chain fatty acids for theirβ-***,the six cryo-electron microscopy structures of ABCD1 in four distinct conformational states were *** the transporter dimer,two transmembrane domains form the substrate translocation pathway,and two nucleotide-binding domains form the ATP-binding site that binds and hydrolyzes *** ABCD1 structures provide a starting point for elucidating the substrate recognition and translocation mechanism of *** of the four inward-facing structures of ABCD1 has a vestibule that opens to the cytosol with variable *** acid(C26:0)-CoA substrate binds to the transmembrane domains(TMDs)and stimulates the ATPase activity of the nucleotide-binding domains(NBDs).W339 from the transmembrane helix 5(TM5)is essential for binding substrate and stimulating ATP hydrolysis by ***1 has a unique C-terminal coiled-coil domain that negatively modulates the ATPase activity of the ***,the structure of ABCD1 in the outward-facing state indicates that ATP molecules pull the two NBDs together and open the TMDs to the peroxisomal lumen for substrate *** five structures provide a view of the substrate transport cycle and mechanistic implication for disease-causing mutations.

关键词： starting hydrolysis insight

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QTL Analysis of the Oil Content and the Hull Content in Brassica napus L.

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Agricultural Sciences in China 2007年第4期6卷 414-421页

作者： JIN Meng-yang li jia-na FU Fu-you ZHANG Zheng-sheng ZHANG Xue-kun liU lie-zhao College of Agronomy and Biochnology Southwest University Beibei 400715 P.R.China Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 P.R.China

The QTLs of the oil content and the hull content were analyzed in Brassica napus L. by constructing the linkage map. The F2：6RIL population with 188 lines, derived from the cross of GH06 × P147, was used as the mapping population. The SRAP, SSR, AFLP, and TRAP markers were used to construct the linkage map, and the composite interval mapping （CIM） to identify the quantitative trait loci associated with the oil content and the hull content. 300 markers were integrated into 19 linkage groups, covering 1 248.5 cM in total. Seven QTLs were found to be responsible for the oil content with the single contribution to phenotypic variance ranging from 3.73 to 10.46%; four QTLs were found for the hull content with the single contribution to phenotypic variance ranging from 4.89 to 6.84%. The yellow-seeded Brassica napus L. has the advantage of higher oil content and the hull content has a significant effect on the oil content. In addition, the SRAP marker is good for detecting QTL.

关键词： Brassica napus L. genetic map molecular markers QTL oil content hull content

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Renal Extramedullary Plasmacytoma--One Case Report

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Clinical oncology and cancer researeh 2010年第6期7卷 380-382页

作者： Yu-ping ZHONG Shi-lun CHEN Xiao-hui liU Ying HU Xin li jia-jia ZHANG na AN Department of Hematology and Oncology Chaoyang Hospital Capital Medical University Research Center of Multiple Myeloma Beijing 100043. China.

Extramedullary plasmacytoma （EMP） is rare in multiple myeloma, especially kidneys are involved. We report one case, including diagnosis and treatment. The purpose of this paper is to provide an attention on the extr... 详细信息

关键词： extramedullary plasmacytoma renal myeloma.

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Improvement of microstructure and mechanical properties of Al−Cu−li−Mg−Zn alloys through water-cooling centrifugal casting technique

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Transactions of Nonferrous Metals Society of China 2024年第11期34卷 3486-3503页

作者： Qing-bo YANG Wen-jing SHI Wen liU Miao WANG Wen-bo WANG li-na jia Hu ZHANG School of Materials Science and Engineering Beihang UniversityBeijing 100191China Research Center for lightweight Materials Ningbo Institute of TechnologyBeihang UniversityNingbo 315100China Suzhou Laboratory Suzhou 215123China

The microstructure and mechanical properties of as-cast Al−Cu−li−Mg−Zn alloys fabricated by conventional gravity casting and centrifugal casting techniques combined with rapid solidification were *** results demonstrated that compared with the gravity casting technique,the water-cooling centrifugal casting technique significantly reduces porosity,refinesα(Al)grains and secondary phases,modifies the morphology of secondary phases,and mitigates both macro-and *** improvements arise from the synergistic effects of the vigorous backflow,centrifugal field,vibration and rapid *** and coarse plate-like Al13Fe4/Al7Cu2Fe phase result in the fracture before the gravity-cast alloy reaches the yield *** centrifugal-cast alloy,however,exhibits an ultra-high yield strength of 292.0 MPa and a moderate elongation of 6.1%.This high yield strength is attributed to solid solution strengthening(SSS)of 225.3 MPa,and grain boundary strengthening(GBS)of 35.7 *** contributes the most to SSS with a scaling factor of 7.9 MPa·wt.%^(-1).The elongation of the centrifugal-cast alloy can be effectively enhanced by reducing the porosity and segregation behavior,refining the microstructure and changing the morphology of secondary phases.

关键词： Al−Cu−li−Mg−Zn alloy water-cooling centrifugal casting microstructure mechanical properties segregation behavior

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Characterization of BrTT1 gene responsible for seed coat color formation in Brassica rapa

Characterization of BrTT1 gene responsible for seed coat col...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会

作者： WANG Yan-hua QU Cun-ming li jia-na Chongqing Engineering Research Center for Rapeseed College of Agronomy and BiotechnologySouthwest University

Yellow seed is a desirable trait in Brassica crops due to its association with higher oil and protein,lower fiber and pigments ***,yellow seed is an important breeding goal for many breeders.A preliminary study suggested that BrTT11,which encodes a WIP zinc finger transcription factor,was responsible for yellow seed formation in Dahuang(a landrace of ***).In this study,cloning and sequence analyses of BrTT1 gene were performed from 22 natural yellow and brown rapeseeds in ***,where gene sequence differences in yellow and brown seed material were found in coding ***-length genomic sequence of BrTTl gene(1 804 bp),together with upstream(1 796 bp) and downstream sequences of target gene(986 bp),was transformed into *** plants were obtained by PCR *** addition,BrTT1 was localized in ***,a ChIP-seq and qRT-PCR analysis were performed on BrTT1 gene to investigate target genes and expression of BrTT1 in flavonoid ***1 gene has different expression levels in different yellow and brown seeds with highly significant *** expression of BrTT1 in yellow-seeded material of *** may cause the aberrant or absent expression of several important BrTT genes of flavonoid biosynthetic pathway,then blocked proanthocyanidin accumulation,and resulted in a transparent testa in ***,forming yellow seed.

关键词： Brassica BrTT1 yellow seed flavonoid biosynthetic

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Extensive tRna gene changes in synthetic Brassica napus between Brassica rapa and Brassica oleracea

Extensive tRNA gene changes in synthetic Brassica napus betw...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Wei li-juan An Ze-shan XiaoMei-li li jia-na Fu Dong-hui Chongqing Engineering Research Center for Rapeseed College of Agronomy and BiotechnologySouthwest University Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of EducationAgronomy CollegeJiangxi Agricultural University

Hybridization or allopolyploidization plays a great role in speciation and genome evolution.A Transfer Rna(abbreviated tRna),typically 73 to 94 nucleotides in length,is indispensable in protein synthesis through transferring an amino acid to the protein synthetic machinery(ribosome).To date,the regularity and function of tRna genes variation during the process of allopolyploidization had been not understood *** this study,the method of inter-tDna corresponding to tRna amplification polymorphism(ITAP) was used to detect tRna-based sequence change in the progeny of interspecific hybrid between *** and B. *** analysis showed that tRna variation in allopolyploidization disrupted the kinship among different accessions,and the diversification of tRna accelerated the differentiation of lines. Significant variation occurred in the early generations of synthetic Brassica napus(F or F generation),but less alterations in the later generation(F generation).These varied tRnas tended to locate in AT-rich *** analysis of varied tRnas against Brassica database showed that the variation of these tRna-based sequences in allopolyploidization might result in the variation of gene structure and function,*** process and *** conclusion,extensive tRna variations were induced in the early stages of the formation of polyploids.

关键词： Synthetic Brassica napus tRna genes allopolyploidization sequence variation

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