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Structural analysis of a shrimp thymidylate synthase reveals species-specific interactions with dUMP and raltitrexed

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Journal of Oceanology and limnology 2020年第6期38卷 1891-1899页

作者： liU Changshui ZANG Kun li Shihao li fuhua MA Qingjun Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266000China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Thymidylate synthase(TS)is a key enzyme in the de novo biosynthesis of thymidine monophosphate,serving as a well-known drug target in chemotherapy against cancers and infectious *** to its clinical value,TS is supposed to be a promising drug target in aquatic-disease *** facilitate designing pathogen-specific TS inhibitors for shrimp-disease control,we report the crystal structures of TS from litopenaeus vannamei(LvTS)in the apo form,LvTS-dUMP complex and LvTS-dUMP-raltitrexed complex at 2.27Å,1.54Å,and 1.56Åresolution,*** shares a similar fold with known TSs,existing as a dimer in the *** apo LvTS and LvTS-dUMP take an open conformation,and raltitrexed binding induces structural changes into a closed conformation in *** to those in other known TS-dUMP-raltitrexed complexes with the closed conformation,the C-terminal loop in LvTS-dUMP-raltitrexed shifts its position away from the bound raltitrexed;the distance between C6 of dUMP and Sγof the catalytic cysteine is obviously longer than that in the known TS structures with closed conformations,resembling that in the TS structures with open *** species-specific interactions with dUMP and raltitrexed are also ***,LvTS-dUMP-raltitrexed adopts a loosely closed conformation with structural features intermediate between the closed and the open conformations that were reported in other *** study provides the first crustcean TS structure,and reveals species-specific interactions between TSs and the ligands,which would facilitate designing pathogen-specific TS inhibitors for shrimp-disease control.

关键词： thymidylate synthase(TS) closed conformation deoxyuridine monophosphate(dUMP) thymidine monophosphate(TMP) raltitrexed litopenaeus vannamei

Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria

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Acta Oceanologica Sinica 2013年第2期32卷 52-58页

作者： SUN Yumiao li fuhua CHI Yanhong XIANG Jianhai Institute of Oceanology Chinese Academy of Sciences Graduate University Chinese Academy of Sciences

Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant pro- tein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrim- p. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI （Resistance index） of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 （P 〈0.05）. The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.

关键词： WSSV VP28 Exopalamon carincauda live bacterial deliver

Heritability estimations of ammonia tolerance and survival of ridgetail white prawn Exopalaemon carinicauda

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Journal of Oceanology and limnology 2022年第4期40卷 1662-1668页

作者： Ding LÜ Chengsong ZHANG Yang YU Jianhai XIANG fuhua li Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China The Innovation of Seed Design Chinese Academy of SciencesWuhan 430072China

Ridgetail white prawn Exopalaemon carinicauda is an important economic marine species in *** improve the germplasm quality of cultured ***,for the fi rst time,we estimated the heritability of ammonia tolerance and survival of ***,covering 263 full-sib families in multiple *** models including binary linear animal model(BLA),binary linear sire-dam model(BLSD),probit threshold animal model(PTA),and probit threshold sire-dam model(PTSD)were *** estimated heritability of tolerance to ammonia in diff erent models ranged from 0.09 to 0.22,and that of survival rate ranged from 0.06 to *** the tolerance to ammonia,both the PTSD and BLSD models showed comparatively better prediction accuracies(0.48 and 0.47,respectively)than PTA and BLA models(0.40 and 0.26,respectively),while to the survival rate,all four models presented relatively low predictive abilities(0.16-0.26).This study provided important information for the future breeding program of ***.

关键词： heritability Exopalaemon carinicauda tolerance to ammonia survival rate

Recent progress of electrochemical reduction of CO_(2)by single atom catalysts

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Materials Reports(Energy) 2022年第3期2卷 3-21页

作者： Tian Wang Jincheng Zhang fuhua li Bin liu Sibudjing Kawi Department of Chemical&Biomolecular Engineering National University of Singapore117585Singapore School of Chemical and Biomedical Engineering Nanyang Technological University637459Singapore

Powered by electricity from renewable energies,electrochemical reduction of CO_(2)could not only efficiently alleviate the excess emission of CO_(2),but also produce many kinds of valuable chemical *** various catalysts,single atom catalysts(SACs)have attracted much attention due to their high atom utilization efficiency and expressive catalytic ***,SACs serve as an ideal platform for the investigation of complex reaction pathways and mechanisms thanks to their explicit active *** this review,the possible re-action pathways for the generation of various products(mainly C1 products for SACs)were firstly ***,recent progress of SACs for electrochemical reduction of CO_(2)was discussed in aspect of different central metal *** the most popular and efficient coordination modulation strategy,introducing heteroatom was then ***,as an extension of SACs,the development of dual atom catalysts was also briefly *** last,some issues and challenges regarding the SACs for CO_(2)reduction reaction(CO_(2)RR)were listed,followed by corresponding suggestions.

关键词： Single atom catalysts CO_(2)reduction reaction Reaction pathways Coordination modulation Dual atom catalysts

Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis

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Acta Oceanologica Sinica 2008年第2期27卷 81-92页

作者： liU Fengsong li fuhua XIANG Jianhai DONG Bo liU Yichen ZHANG Xiaojun ZHANG liusuo College of life Science Hebei University Baoding 071002 China Experimental Marine Biology Laboratory Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fennero- penaeus chinensis by 3 ′and 5′ RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12. 3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus （ litopenaeus ） setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary elec- trophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen.

关键词： Crustin-like gene Fenneropenaeius chinensis molecular cloning expression analysis microbe challenge

Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus

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Journal of Oceanology and limnology 2018年第6期36卷 2368-2378页

作者： DUAN Hu JIN Songjun li fuhua ZHANG Xiaojun XIANG Jianhai Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology

As in vertebrates, brains play key roles in rhythmic regulation, neuronal maintenance, diff erentiation and function, and control of the release of hormones in arthropods. But the structure and functional domains of the brain are still not very clear in crustaceans. In the present study, we reveal the structural details of the brain in the redclaw crayfish using hematoxylin-eosin staining and microscopic examination, firstly. The brain of crayfish is consist of three main parts, namely, protocerebrum, deutocerebrum, and tritocerebrum, including some tracts and commissures, briefly. Secondly, at least 9 kinds of brain cells were identified on the basis of topology and cell shapes, as well as antibody labeling. We also provide morphological details of most cell types, which were previously un-described. In general, four types of glia and three types of neurosecretory cells were described except cluster 9/11 and cluster 10 cells. Glia were categorized into another three main kinds:(1) surface glia;(2) cortex glia; and(3) neuropile glia in addition to astrocytes identified by GFAP labelling. And neurosecretory cells were categorized into I, Ⅱ and III types based on morphological observation. Finally, cluster 9/11 and 10 cells derived from the brain of crayfish, could be used for primary culture about 7–9 d under the optimized conditions. There results provide a resource for improving the knowledge of the still incompletely defined neuroendocrinology of this species. Using the crayfish as an animal model, we are easy to carry out further research in manipulating their endocrine system, exploring cellular and synaptic mechanisms so much as larval production on a small scale, such as in a cell or tissue.

关键词： Cherax quadricarinatus brain’s structure brain cells primary culture

Stability of potentially toxic elements in municipal sludge biochars modified by MgCl2 and phosphate

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Waste Disposal and Sustainable Energy 2023年第1期5卷 13-23页

作者： Qiqi Huang Siqi Chen Jinhao lin Jingzi Beiyuan Jin Wang Juan liu Yundang Wu Xiaolian Wu fuhua li Wenbing Yuan Chengrong Nie School of Environmental and Chemical Engineering Foshan UniversityFoshan528000China School of Food Science and Technology Foshan UniversityFoshan528000China Foshan Engineering and Technology Research Center for Contaminated Soil Remediation School of Environmental and Chemical EngineeringFoshan UniversityFoshan528000China School of Environmental Science and Engineering Key Laboratory of Water Quality and Conservation in the Pearl River DeltaMinistry of EducationGuangzhou UniversityGuangzhou510006China Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management Institute of Eco-Environmental and Soil SciencesGuangdong Academy of SciencesNational-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South ChinaGuangzhou510650China

Municipal wastewater sludge can be pyrolyzed as biochars to better use nutrients and stabilize carbon compared with other typical technologies,such as landfill and ***,sludge-derived biochars might contain large amounts of potentially toxic elements(PTEs),such as Zn,Cu,Cr,Ni,Pb,and *** stability of PTEs in biochars might be improved by higher pyrolytic temperatures,which can be further improved by different ***,PO4-modification at 300°C and Cl-modification at 700°C were carried out,respectively,to enhance the stability of *** leaching tests have been performed to assess the stability of PTEs in biochars,including the synthetic precipitation leaching procedure(SPLP),toxicity characteristic leaching procedure(TCLP),diethylenetriamine pentaacetate(DTPA)extraction,and in vitro simple bioaccessibility extraction test(SBET).The morphological structure,elemental mapping,and mineral formation of the pristine and modified biochars were studied by scanning electron microscopy–energy-dispersive X-ray spectroscopy(SEM–EDS)and X-ray diffraction(XRD).Our results suggested that the leachability,mobility,plant-availability,and bioaccessibility of most PTEs were decreased by pyrolysis,yet the total contents of PTEs were elevated,especially at 700°***,modification by phosphates and MgCl2 enhanced the stability of PTEs in ***,it should be noted that higher bioaccessibility of PTEs was observed in biochars of P-modification than Cl-modification,which is associated with the dissolution of phosphate precipitates under acidic conditions(pH<2).Additionally,Cl-modification leads to higher plant-available Zn and Cu and bioaccessible Zn compared with the unmodified biochar produced at 700°C.

关键词： Biochar modification Sludge biochar Sewage sludge utilization Chlorination Pyrolysis Heavy metals

论党的教育方针及其在高等学校贯彻落实的主要路径

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中国高教研究 2019年第3期 66-71页

作者：李福华青岛大学师范学院院长教授山东青岛266071

教育方针是国家执政党在一定历史阶段指导教育事业发展的行动纲领和战略原则,决定教育的性质、目的、地位与基本政策。我国教育方针既保持了贯穿始终的"培养全面发展的社会主义事业建设者和接班人"精神,也不断结合时势适当调整着内容。... 详细信息

教育方针是国家执政党在一定历史阶段指导教育事业发展的行动纲领和战略原则,决定教育的性质、目的、地位与基本政策。我国教育方针既保持了贯穿始终的"培养全面发展的社会主义事业建设者和接班人"精神,也不断结合时势适当调整着内容。党的十八大以来,习近平总书记发表的系列重要讲话进一步阐明了我们党的教育方针,高等学校应不断增强对党的教育方针宣传力度,并且在人才培养的全过程、高校管理的各方面和办学考核评估中贯彻落实党的教育方针。

关键词：教育方针高等学校贯彻落实

关于羊子山土台遗址和几件出土文物的历史价值问题

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四川文物 2010年第1期 43-46页

作者：李复华四川博物院

本文认为成都羊子山土台之功效为灵台,是古代帝王得民心得天下不可或缺的重要建筑,并联系到金沙遗址玉琮、玉璧,认为两者可等量齐观,均与杜宇称帝及祭祀有关。文中涉及的东汉养老画像砖不仅再现了汉代对庶老的养老情景,同时亦是对秦前... 详细信息

本文认为成都羊子山土台之功效为灵台,是古代帝王得民心得天下不可或缺的重要建筑,并联系到金沙遗址玉琮、玉璧,认为两者可等量齐观,均与杜宇称帝及祭祀有关。文中涉及的东汉养老画像砖不仅再现了汉代对庶老的养老情景,同时亦是对秦前期庶老礼制的承袭和印证。

关键词：文物历史价值