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Modified Da-chai-hu Decoction regulates the expression of occludin and NF-κB to alleviate organ injury in severe acute pancreatitis rats

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Chinese Journal of Natural Medicines 2019年第5期17卷 355-362页

作者： ZHAO Guang ZHUO Yu-Zhen CUI li-hua li Cai-Xia CHEN Sha-Yan li Dan liU Jun-Hong li di-hua CUI Nai-Qiang ZHANG Shu-Kun Hepatobiliary and Pancreatic Surgery Tianjin Nankai Hospital Tianjin Hospital of Integrated Traditional Chinese and Western medicine Nankai Clinical College Tianjin Medical University Institute of Integrated Traditional Chinese and Western medicine Tianjin Nankai Hospital Tianjin Hospital of Integrated Traditional Chinese and Western medicine Department of Clinical Laboratory Tianjin Nankai Hospital Tianjin Hospital of Integrated Traditional Chinese and Western medicine

Modified Da-chai-hu Decoction(MDD), a traditional Chinese medicinal formulation, which was empirically generated from Da-chai-hu decoction, has been utilized to treat severe acute pancreatitis(SAP) for decades. The aim of the present study was to explore its potential organprotective mechanism in SAP. In the present study, rat SAP model was induced by retrograde injection of 3.5% sodium taurocholate into the biliopancreatic duct, MDD(23.35 g/kg body weight, twelve times the clinical dose) were orally given at 2 h before and 10 h after injection. At 12 h after model induction, blood was taken from vena cava for analysis of amylase, diamine oxidase(DAO), pulmonary surfactant protein-A(SP-A), and C-reactive protein(CRP). Histopathological change of pancreas, ileum and lung was assayed by H&E staining, myeloperoxidase(MPO) activity were determinated using colorimetric assay, and the expressions of occludin and nuclear factor-κB(NF-κB) were detected by real-time RT-PCR and western blot, respectively. In addition, the tissue concentrations of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and monocyte chemoattractant protein-1(MCP-1) were measured by enzyme-linked immunosorbent assay(EliSA). The results showed that in SAP rats, MDD significantly alleviated histopathological damage, depressed the MPO activity and the concentrations of TNF-α, IL-1β, and MCP-1 of pancreas, ileum and lung, and reduced the serum levels of amylase [(3283.4±585.5) U·L^(-1) vs(5626.4±795.1) U·L^(-1)], DAO[(1100.1±334.3) U·L^(-1) vs(1666.4±525.3) U·L^(-1)] and CRP [(7.6±1.2) μg·mL^(-1) vs(17.8±3.8) μg·mL^(-1)]. However, the serum SP-A concentration [(106.1±16.6) pg·mL^(-1) vs(90.1±14.9) pg·mL^(-1)] was elevated when treated SAP rats with MDD. Furthermore, MDD increased the occludin expression and reduced the NF-κB expression in pancreas, ileum and lung of SAP rats. Our findings suggested that MDD administration was an effective therapeutic approach for SAP treatment. It could up-regulate o

关键词： Severe acute pancreatitis Modified Da-chai-hu Decoction Occludin Nuclear factor-κB Ileum Lung

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UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells

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The Journal of Biomedical Research 2011年第2期25卷 135-140页

作者： Wei li Wu di lijuan hua Bingrong Zhou ze Guo Dan Luo Department of Dermatology the First Affiliated Hospital of Nanjing Medical University Nanjing Jiansu 210029 China

MicroRNAs （miRNAs） are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B （UVB） radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 （PTEN） expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucleotide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB irradiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.

关键词： MicroRNA-141 PTEN UVB apoptosis HaCaT cells

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Modified Xiaochaihu Decoction(加味小柴胡汤) Promotes Collagen Degradation and Inhibits Pancreatic Fibrosis in Chronic Pancreatitis Rats

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Chinese Journal of Integrative Medicine 2020年第8期26卷 599-603页

作者： ZHANG Shu-kun CUI Nai-qiang ZHUO Yu-zhen HU Jian-gong liU Jun-hong li di-hua CUI li-hua Department of Cell and Molecular Biology Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western MedicineTianjin Nankai HospitalTianjin 300100China Department of the First Biliary and Pancreatic Surgery Tianjin Nankai HospitalTianjin 300100China Department of Pathology the Second Affiliated Hospital of Tianjin University of Traditional Chinese MedicineTianjin 300150China Department of Pharmacological Institute of Acute Abdominal Diseases of Intergrated Traditional Chinese and Western MedicineTianjin Nankai HospitalTianjin 300100China

Objective:To investigate the effect of Modified Xiaochaihu Decoction(MXD,加味小柴胡汤)on collagen degradation in rats with chronic pancreatitis(CP).Methods:Rats were injected dibutyltin dichloride(DBTC,7 mg/kg of body weight)into the right caudal vein to induce CP *** heallhy male Wistar rats were randomly divided into three groups by a random number table:the control,the model and the treatment *** of treatment group were administered MXD(10 g/kg of body weight)orally once daily starting from the day post-model *** tissues were harvested after 28-day feeding and fibrosis was evaluated by picro-sirius red *** contents of collagen typeⅠandⅢwere detected using enzymelinked immunosorbent assay(EliSA),the expression of matrix metalloproteinase 13(MMP13)and tissue inhibitor of metalloproteinase 1(TIMP1)was analyzed by Western blot and real-time polymerase chain reaction(PCR).Results:The fibrosis scoring of pancreatic tissues,the concentrations of collagen typeⅠandⅢ,the expression levels of MMP13 and TIMP1 proteins and mRNA in the model group were all increased compared with the control group(Pdifferences in the expression levels of TIMP1 proteins and m RNA(P>0.05).Conclusion:MXD could promote collagen degradation and reverse pancreatic fibrosis in CP rats via a mechanism involve up-regulation of MMP13 expression.

关键词： Modified Xiaochaihu Decoction collagen degradation chronic pancreatitis rats matrix metalloproteinase 13 tissue inhibitor of metalloproteinase 1

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Low Correspondence of EGFR Mutations in Tumor Tissue And Paired Serum of Non-Small-Cell Lung Cancer Patients

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Chinese Journal of Cancer Research 2010年第1期22卷 27-31页

作者： Guo-hong Song,Jun Ren,li-jian Zhang,li-jun di, Yan-hua Yuan,Jing Yu,Jun Jia Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Medical Oncology,Peking University School of Oncology,Beijing Cancer Hospital & Institute,Beijing 100142,China Key laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Medical Oncology Peking University School of Oncology Beijing Cancer Hospital & Institute Beijing China

Objective： Epidermal growth factor receptor （EGFR） mutations are strong determinants of tumor response to EGFR tyrosine kinase inhibitors in non-small-cell lung cancer （NSCLC） patients. The aim of this study was to evaluate the correspondence between EGFR mutations in non-small-cell lung cancer tissues and in circulating DNA. Methods： The research was conducted in 50 non-small-cell lung cancer patients who had undergone curative surgery, and in whom both serum and neoplastic tissues were available. Meanwhile sera of 33 cases of advanced NSCLC patients were also analyzed. DNA were extracted from each sample. Mutations of EGFR in exonl8-21 were examined by PCR amplification method and direct sequencing. Results： EGFR mutations were detected in 15 （30%） of 50 neoplastic tissue samples, 6 cases were in-frame deletion del E746-A750 in exonl9, 9 cases were substitution in exon 21 （all were L858R except one was L861Q）, but no mutated DNA resulted in paired serum circulating DNA samples of 50 resectable patients. As the 33 advanced NSCLC patients, EGFR mutations were detected in only 2 serum circulating DNA samples, all were L858R mutation in exon 21. Conclusion： These data indicated that it was difficult to identify EGFR mutations in circulating DNA of NSCLC patients. The use of EGFR mutation in serum as a clinical method for decision making of TKI therapy is unsatisfactory.

关键词： Circulating DNA Epidermal growth factor receptor (EGFR) Mutation Non-small-cell lung cancer (NSCLC)

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CIMICIFUGAMIDE AND ISOCIMICIFUGAMIDE,TWO NEW CINNAMAMIDE DERIVATIVES ISOLATED FROM CIMICIFUGA DAHURICA

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Chinese Chemical Letters 1993年第10期4卷 891-892页

作者： Cong Jun li di hua CHEN Pei Gen XIAO Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100094

Two new cinnamamide derivatives designated as cimicifugamide((?))and isocimicifu- gamide((?)),have been isolated from the rhizomes of Cimicifuga dahurica(Turcz.)Maxim..Their structures were established based on spectral evidence as N-(3'-methoxy-4'-hydroxyphenethyl)- 4-0-β-D-galactopyrano syl-ferulamide(E)and N-(3'-methoxy-4'-hydroxyphenetnyl)-4-0-β-D-ga- lactopyranosyl-isoferulamide(Z)respectively.

关键词： CIMICIFUGAMIDE AND ISOCIMICIFUGAMIDE,TWO NEW CINNAMAMIDE DERIVATIVES ISOLATED FROM CIMICIFUGA DAHURICA Ga

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NEW CYCLOARTANE TRITERPENOID CIMISIDE E FROM CIMICIFUGA FOETIDA L.

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Chinese Chemical Letters 1993年第11期4卷 957-958页

作者： Cong Jun liYing He li di hua CHEN Pei Gen XIAO Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100094

This paper relates to the structural elucidation of a new cycloartane triterpene glycoside, namely cimiside E(1), which was newly isolatea from the rnizomes of Cimicifuga foetida L.. Based on spectral evidence, its st... 详细信息

关键词： Chen NEW CYCLOARTANE TRITERPENOID CIMISIDE E FROM CIMICIFUGA FOETIDA L

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Local magnetic moment oscillation around an Anderson impurity on graphene

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 192-201页

作者： Shuai li Zhen Ma Jin-hua Gao School of Science Hebei University of Science and Technology School of Electronics and Information Zhengzhou University of Light Industry School of Physics and Wuhan National High Magnetic Field Center Huazhong University of Science and Technology

We theoretically investigate the spin resolved Friedel oscillation （FO） and quasiparticle interference （QPI） in graphene induced by an Anderson *** the impurity becomes magnetic,the resulted FO becomes spin dependent,which gives rise to a local magnetic moment oscillation with an envelop decaying as r-2in real space in the doping ***,at half filling,the electron density and local magnetic moment will not oscillate but decay as *** spin resolved FO has both sublattice and spin ***,the local magnetic moment decay at half filling only occurs at one sublattice of graphene,which is quite like the phenomenon observed in the STM experiment (***ález-Herrero et al.,Science 352,437 （2016）).We further give an analytic formula about such spin dependent FO based on the stationary phase ***,we study the interference of quasiparticles around the magnetic impurity by calculating the spin dependent Fourier-transformed local density of states （FT-LDOS）.Our work gives a comprehensive understanding about the local magnetic moment oscillation around an Anderson impurity on graphene.

关键词： Friedel oscillation local magnetic moment graphene Anderson impurity

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景观规划中的生态廊道宽度

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生态学报 2005年第9期25卷 2406-2412页

作者：朱强俞孔坚李迪华北京大学景观设计学研究院北京100871

生态廊道具有保护生物多样性、过滤污染物、防止水土流失、防风固沙、调控洪水等多种功能。建立生态廊道是景观生态规划的重要方法,是解决当前人类剧烈活动造成的景观破碎化以及随之而来的众多环境问题的重要措施。按照生态廊道的主要... 详细信息

生态廊道具有保护生物多样性、过滤污染物、防止水土流失、防风固沙、调控洪水等多种功能。建立生态廊道是景观生态规划的重要方法,是解决当前人类剧烈活动造成的景观破碎化以及随之而来的众多环境问题的重要措施。按照生态廊道的主要结构与功能,可将其分为线状生态廊道、带状生态廊道和河流廊道3种类型。生态廊道设计包括的关键问题有廊道数目、本底、宽度、联接度、构成要素、关键点(区)等。由于生态廊道结构与功能的复杂性,使得廊道的宽度具有很大的不确定性。具体的讲,生态廊道的宽度由保护目标、植被情况、廊道功能、周围土地利用,廊道长度等多个因素决定。合适的廊道宽度应该根据对廊道主要生态过程的研究来确定。从景观的结构与功能分析出发,分别从生物保护廊道和河流廊道两方面对生态廊道的宽度及其影响因素进行分析,并对相关研究成果进行综述,总结得出两种类型生态廊道的适宜宽度值范围。最后提出确定宽度时应该注意的相关问题。

关键词：景观规划景观生态生态廊道宽度河流廊道

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镇江海绵城市建设老旧小区改造中的挑战与对策

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中国给水排水 2020年第24期36卷 34-38页

作者：仲笑林李迪华北京大学建筑与景观设计学院北京100871

镇江作为中国海绵城市建设第一批试点城市之一,依托海绵城市建设的建成区户外环境改造是其最大特色。镇江建成区中老旧小区分布范围广,环境极为复杂,与广大居民生活息息相关,改造难度大。探讨这些难题的解决办法是建成区海绵城市建设工... 详细信息

镇江作为中国海绵城市建设第一批试点城市之一,依托海绵城市建设的建成区户外环境改造是其最大特色。镇江建成区中老旧小区分布范围广,环境极为复杂,与广大居民生活息息相关,改造难度大。探讨这些难题的解决办法是建成区海绵城市建设工作的重中之重。通过分析镇江老旧小区建设实例,归纳镇江老旧小区改造当中遇到的特殊挑战,同时从雨水工程与景观建设两个层次提出解决策略,研究可供中国海绵城市建设在老旧小区改造实践中参考借鉴。

关键词：海绵城市建设老旧小区改造低影响开发

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北京市生态安全格局及城市增长预景

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生态学报 2009年第3期29卷 1189-1204页

作者：俞孔坚王思思李迪华李春波北京大学景观设计学研究院北京100871

在快速城市化和城市生态安全面临巨大挑战的时代背景下,构建生态安全格局是实现区域和城市生态安全的基本保障和重要途径。在梳理国内外生态安全格局研究进展的基础上,提出基于景观安全格局理论的北京市生态安全格局网络和城市发展空间... 详细信息

在快速城市化和城市生态安全面临巨大挑战的时代背景下,构建生态安全格局是实现区域和城市生态安全的基本保障和重要途径。在梳理国内外生态安全格局研究进展的基础上,提出基于景观安全格局理论的北京市生态安全格局网络和城市发展空间格局。通过对北京市水文、地质灾害、生物多样性保护、文化遗产和游憩过程的系统分析,运用GIS和空间分析技术,判别出维护上述各种过程安全的关键性空间格局(景观安全格局),进而综合、叠加各单一过程的安全格局,构建具有不同安全水平的综合生态安全格局,形成保障北京城市和区域生态安全的生态基础设施。在此基础上,提出城镇空间发展预景和土地利用空间布局的优化战略。对不同预景进行综合评价,结果显示:基于生态安全格局的城镇空间发展格局可以维持生态过程的连续性并克服常规城市发展模式下的蔓延,基于"满意生态安全格局"(中安全水平)的城市空间格局可以同时满足生态用地、农用地和建设用地的需求,是一个同时实现精明保护与精明增长的有效工具。

关键词：生态安全格局生态基础设施景观安全格局土地利用北京

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