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海水中溶解氨基酸分析技术的研究进展

海洋环境科学 2020年第6期39卷 960-967页

作者：袁蕾张纯超吕彦儒国家海洋局南海环境监测中心广东广州510300

随着对海洋中有机氮生物地球化学循环过程认识的逐步深入,氨基酸作为海洋有机氮库中最易被生物利用的组分之一愈加受到重视。关于海洋中氨基酸含量组成和生物可利用性的相关研究对认识海洋中有机氮的迁移和转化具有重要意义。本文详细... 详细信息

随着对海洋中有机氮生物地球化学循环过程认识的逐步深入,氨基酸作为海洋有机氮库中最易被生物利用的组分之一愈加受到重视。关于海洋中氨基酸含量组成和生物可利用性的相关研究对认识海洋中有机氮的迁移和转化具有重要意义。本文详细阐述了海洋领域分光法和荧光法等溶解氨基酸测定方法及离子色谱和液相色谱等色谱分离方法的发展历程,并对应用最普遍的柱前衍生-反相液相色谱-荧光检测法等技术进行了更为详细的评述,列举了质谱技术、单体同位素分析技术和毛细管电泳等最新发展的技术方法,最后对其在海洋学中的应用进行展望。

关键词：氨基酸荧光色谱法衍生化

Genome-wide identification of key regulatory lncRNAs in esophageal cancer metastasis

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Signal Transduction and Targeted Therapy 2021年第3期6卷 621-623页

作者： Wen Wen Xu Can-Can Zheng Qian Zuo Jun-Qi Li Pan Hong yan-ru Qin Xin-Yuan Guan Qing-Yu He Hua-Xin Liao Bin Li MOE Key Laboratory of Tumor Molecular Biology and Guangdong Provincial Key Laboratory of Bioengineering Medicine National Engineering Research Center of Genetic MedicineInstitute of BiomedicineCollege of Life Science and TechnologyJinan UniversityGuangzhouChina MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health EngineeringCollege of Life Science and TechnologyJinan UniversityGuangzhouChina State Key Laboratory of Esophageal Cancer Prevention and Treatment Department of Clinical OncologyFirst Affiliated HospitalZhengzhou UniversityZhengzhouChina Department of Clinical Oncology Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong SARChina

Dear Editor,Metastasis leads to a poor prognosis of patients with esophageal squamous cell carcinoma(ESCC).1-3 but the study on cancer metastasis has been hampered by a lack of reliable cell and animal *** identification and functional validation of metastasis-associated long non-coding RNAs(lncRNAs)and microRNAs(miRNAs),as well as-their interactions in ESCC are urgently needed.

关键词： metastasis esophageal cancer

Synthesis, Structure Characterization, and Cu^(2+) Recognition of 3-{[3-(Phenylsulfonamido)benzoyl]methylidene}-3,4-dihydroquinoxaline-2(1H)-one

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Chemical Research in Chinese Universities 2006年第6期22卷 747-752页

作者： LI Xue-mei ZENG Cheng-chu NIU Li-ting yan Hong ZHENG Da-wei ZHONG ru-gang College of Life Science ＆ Bioengineering Beijing University of Technology Beijing 100022 P. R. China

Aryl diketo acid derivatives are one of the most promising HIV-1 integrase（IN） inhibitors. With a view to substitute the critical diketo acid pharmacophore with the diketo benzimidazole unit, the coupling reaction of compound 4 with o-phenylenediamine was carried out. However, the reaction product, compound 5, was confirmed to be 3-{ [ 3- （phenylsulfonamido） benzoyl] methylidene t -3,4-dihydroquinoxaline-2 （1H） -one rather than the 2-benzimidazole derivative by using X-ray diffraction. Owing to its low solubility in water, the evaluation of the anti-HIV IN activity of the synthesized compound 5 could not be carried out. Consequently, the ion-binding properties of compound 5 in the absence of HIV-1 IN were investigated with UV-Vis spectroscopy in organic solvents. The results show that such a compound can selectively recognize Cu^2＋.

关键词： Diketo acid Quinoxalone derivative X-ray crystal structure Cu^2+ recognition

Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model

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Horticulture Research 2020年第1期7卷 2328-2341页

作者： Wenfang Zeng Liang Niu Zhaohui Wang Xiaobei Wang yan Wang lei Pan Zhenhua Lu Guochao Cui Weining Weng Mingqiao Wang Xun Meng Zhiqiang Wang Author details 1Zhengzhou Fruit Research Institute Chinese Academy of Agricultural Sciences450009 ZhengzhouChina Abmart 200233 ShanghaiChina Northwest University 710127 Xi’anChina

Peach(Prunus persica)is a typical climacteric fruit that produces ethylene rapidly during ripening,and its fruit softens *** hard peach cultivars,however,do not produce large amounts of ethylene,and the fruit remains firm until fully ripe,thus differing from melting flesh peach *** identify the key proteins involved in peach fruit ripening,an antibody-based proteomic analysis was conducted.A mega-monoclonal antibody(mAb)library was generated and arrayed on a chip(mAbArray)at a high density,covering~4950 different proteins of *** the screening of peach fruit proteins with the mAbArray chip,differentially expressed proteins recognized by 1587 mAbs were identified,and 33 corresponding antigens were ultimately identified by immunoprecipitation and mass *** proteins included not only important enzymes involved in ethylene biosynthesis,such as ACO1,SAHH,SAMS,and MetE,but also novel factors such as ***,protein–protein interaction analysis identified a metabolon containing SAHH and *** combining the antibody-based proteomic data with the transcriptomic and metabolic data,a mathematical model of ethylene biosynthesis in peach was *** results showed that MetE is an important regulator during peach ripening,partially through interaction with SAHH.

关键词： peach cycle screening

基于SBAS-InSAR的开采沉陷特征分析

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陕西煤炭 2024年第6期43卷 34-38页

作者：孙超徐海璐潘磊陕西建新煤化有限责任公司陕西延安727300

针对传统地表沉陷监测方法在开采沉陷影响范围识别中的不足,以建新煤矿为例,采用SBAS-InSAR技术对覆盖研究区域的2021年12月9日至2022年6月2日的30景重访周期为12 d的Sentinel-1A数据进行干涉处理,得到了采空区沉陷范围,并对时空分布特... 详细信息

针对传统地表沉陷监测方法在开采沉陷影响范围识别中的不足,以建新煤矿为例,采用SBAS-InSAR技术对覆盖研究区域的2021年12月9日至2022年6月2日的30景重访周期为12 d的Sentinel-1A数据进行干涉处理,得到了采空区沉陷范围,并对时空分布特征进行分析。结果表明,该区域主要分布3处沉降中心,形变影响范围约为3.77 km^(2);最大累计沉降量为-530 mm,年平均形变速率最大达到-266 mm/a;时序沉降值与GNSS实测值整体趋势一致。该方法证明了短重访SAR数据可动态提取矿区时空分布特征以及时序变化过程,能够为矿区灾害防治提供科学依据。

关键词： SBAS-InSAR 煤矿开采开采沉陷沉降速率形变速率

Molecular Characterization and Correlation with β-lactam Resistance of Streptococcus pneumonia Isolates in Hangzhou,China

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Biomedical and Environmental Sciences 2018年第5期31卷 389-393页

作者： CHU Mei Fen LIU Xiao Xiang ZHANG Shao Ni HUANG yan Ying2 3 DU Peng2 WANG Li Fang2 JI lei2 yan Jie4 5 SUN Ai Hua2 department of laboratory medicine hangzhou medical collegeHangzhou 310053ZhejiangChina faculty of basic medicine hangzhou medical collegeHangzhou 310053ZhejiangChina school of laboratory medical science and life science wenzhou medical universityWenzhou 325035ZhejiangChina division of basic medical microbiology state key laboratory for diagnosis and treatment of infectious diseasesfirst affiliated hospital of zhejiang universityHangzhou 310003ZhejiangChina department of medical microbiology and parasitology college of medicinezhejiang universityHangzhou 310058ZhejiangChina

Penicillin-binding proteins（PBPs） are the target of β-lactam antibiotics（the major treatment for Streptococcus pneumoniae infections）,and mutations in PBPs are considered as a primary mechanism for the development of β-lactam resistance in *** study was conducted to investigate the mutations in the PBPs of clinical *** isolates in Hangzhou,China,in correlation with β-lactam *** showed that 19 F was the predominant serotype（7/27） and 14 of the *** isolates were resistant to both penicillin G and *** results suggested that β-lactam-resistant isolates primarily exhibited single-site mutations in both the STMK and SRNVP motifs of pbp1 a in combination with double-site mutations in the STMK motif of pbp2 x,which might be the primary mechanisms underlying the β-lactam resistance of the isolates in this study.

关键词： PBP lactam Resistance of Streptococcus pneumonia Isolates in Hangzhou,China RSP

Assessment of duodenogastric reflux by combined continuous intragastric pH and bilirubin monitoring

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World Journal of Gastroenterology 2002年第2期8卷 382-384页

作者： Fei Dai Jun Gong ru Zhang Jin-yan Luo You-Ling Zhu Xue-Qin Wang,Department of Gastroenterology,Second Hospital of Xi’ an Jiaotong University,Xi’an 710004,Shaanxi Province,China Department of Gastroenterology Second Hospital of Xi'an Jiaotong University 157 Xiwu LU Xi'an 710004 Shaanxi Province China. jiangdf@***

AIM: To assess the diagnostic value of a combination of continuous intragastric pH and bilirubin monitoring in the detection of duodenogastric reflux (DGR), and the effects of diet on the bilirubin absorbance. METHODS: 30 healthy volunteers were divided into two groups: standard diet group (Group 1) 18 cases, free diet group (Group 2)12 cases. Each subjects were subjected to simultaneous 24-hour intragastric pH and spectrophotometric bilirubin concentration monitoring (Bilitec 2000). RESULTS: There was no difference of preprandial phase bilirubin absorbance between two groups. The absorbance of postprandial phase was significantly increased in group 2 than group 1. There was no difference between preprandial phase and postprandial phase absorbance in group 1. Postprandial phase absorbance was significantly higher in group 2. In a comparison of bile reflux with intragastric pH during night time, there were 4 types of reflux: Simultaneous increase in absorbance and pH in only 19.6%, increase in bilirubin with unchanged pH 33.3%, pH increase with unchanged absorbance 36.3%, and both unchanged in 10.8%. Linear regression analysis showed no correlation between percentage total time of pH0.14, r=0.068 Prubin monitoring separately predict the presence of duodenal (and/or pancreatic) reflux and bile reflux. They can not substitute for each other. The detection of DGR is improved if the two parameters are combined simultaneously.

关键词： Adult Aged Bilirubin Diet Duodenogastric Reflux Female Fiber Optics Humans Hydrogen-Ion Concentration Male Middle Aged Postprandial Period Random Allocation Research Support, Non-U.S. Gov't

Enterococcus faecium NCIMB 10415 administration improves the intestinal health and immunity in neonatal piglets infected by enterotoxigenic Escherichia coli K88

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Journal of Animal Science and Biotechnology 2020年第1期11卷 132-146页

作者： Xie Peng ru Wang Liang Hu Qiang Zhou yang Liu Min yang Zhengfeng Fang yan Lin Shengyu Xu Bin Feng Jian Li Xuemei Jiang Yong Zhuo Hua Li De Wu Lianqiang Che Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China Institute of Animal NutritionSichuan Agricultural UniversityChengduSichuan 611130People’s Republic of China Animal Husbandry and Veterinary Department Chengdu Agricultural CollegeChengduSichuan 611130People’s Republic of China.

Background:This study aimed to investigate the effects of oral administration of Enterococcus faecium NCIMB10415(***)on intestinal development,immunological parameters and gut microbiota of neonatal piglets challenged with enterotoxigenic Escherichia coli K88(ETEC).A total of 961-day-old sow-reared piglets were randomly assigned to 2 groups,with 48 piglets in each *** piglets were from 16 litters(6 piglets each litter),and 3 piglets each litter were allocated to the ***-supplemented(PRO)group,while the other 3 piglets were allocated to the control(CON)*** colostrum intake,piglets in the PRO group were orally administrated with 3×10~9 CFU *** per day for a period of one *** day 8,one piglet per litter from each group was challenged(CON+ETEC,PRO+ETEC)or not(CON-ETEC,PRO-ETEC)with ETEC in a 2×2 factorial arrangement of *** day 10(2 days after challenge),blood and tissue samples were obtained from ***:Before ETEC challenge,there were no significant differences for the average daily gain(ADG)and fecal score between the two groups of *** ETEC challenge,the challenged piglets had greater fecal score compared to the non-challenged piglets,whereas *** administration was able to decrease the fecal *** challenged with ETEC had shorter villous height,deeper crypt depth,and reduced number of goblet cells in the jejunum and decreased m RNA abundance of claudin-1 in the ileum,whereas increased the percentage of lymphocytes,concentrations of IL-1βin the plasma and TNF-αin the ileal mucosa,as well as increased the m RNA abundances of innate immunity-related genes in the ileum *** deleterious effects caused by ETEC were partly alleviated by feeding *** addition,piglets in PRO-ETEC group had decreased the percentage of CD8^+T cells of the peripheral blood when compared to those in CON-ETEC ***,*** administration increased Verrucomicrobia at phylum level and decreased

关键词： Enterococcus faecium Enterotoxigenic Escherichia coli K88 Gut microbiota Immunity Intestine Neonatal piglets

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应用于干涉显微镜的直线压电作动器

光学精密工程 2013年第6期21卷 1524-1530页

作者：朱华曹如意菅磊南京航空航天大学机械结构力学与控制国家重点实验室江苏南京210016

提出了应用于干涉显微镜焦距调节的直线压电叠堆作动器和微动台。介绍了基于三角位移放大原理的压电作动器结构设计,利用ANSYS的APDL语言实现了对作动器钢架结构的建模,并采用Optimus中自带的差分进化算法(DE)对其结构尺寸进行了优化。... 详细信息

提出了应用于干涉显微镜焦距调节的直线压电叠堆作动器和微动台。介绍了基于三角位移放大原理的压电作动器结构设计,利用ANSYS的APDL语言实现了对作动器钢架结构的建模,并采用Optimus中自带的差分进化算法(DE)对其结构尺寸进行了优化。制作了实验样机,激光干涉实验表明:当驱动电压信号幅值为40～100V时,作动器位移放大倍数可以达到7。最后,将设计的直线作动器作为驱动核心安装在自行设计的微动台上,然后将组成的系统用于光学干涉显微镜。实验显示,整个系统在电压为24～40V,阶梯增量电压为0.8V时,步进分辨率可达23nm,满足干涉显微镜细分干涉条纹所需要的直线位移分辨率的要求。

关键词：干涉显微镜三角放大原理步进式压电作动器结构优化干涉条纹