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Biomedical and Environmental Sciences 2018年第3期31卷 227-232页

作者： HUANG Xiao Xia YAN lei GAO Xiao Yan REN Yu Huan FU Shi Hong CAO Yu Xi HE Ying lei wen wen LIANG Guo Dong WANG Shi wen WANG Huan Yu Office for Disease Control and Prevention National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention Harmacoepidemiology Asia-Pacific unit MSD R&D(China) Co.Ltd. Department of Viral Encephalitis National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and Prevention Institute of Remote Sensing and Digital Earth Chinese Academy of Sciences

Japanese encephalitis（JE）is a serious public health *** study was undertaken to better understand the relationship between JE distribution and environmental factors in *** data from 2005 to 2010 were retrieved from ... 详细信息

关键词： Japanese encephalitis Environmental factors Landscape

A Reverse-transcription Recombinase-aided Amplification Assay for the Rapid Detection of the Far-Eastern Subtype of Tick-borne Encephalitis Virus

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Biomedical and Environmental Sciences 2019年第5期32卷 357-362页

作者： WANG Qian Ying LI Fan SHEN Xin Xin FU Shi Hong HE Ying lei wen wen LIANG Guo Dong WANG Huan Yun MA Xue Jun Department of Viral Encephalitis NHC Key Laboratory of BiosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and PreventionBeijing 102206China NHC Key Laboratory of Medical Virology and Viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease. Methods A reverse-transcription recombinase-aided amplification(RT-RAA) assay was developed. This assay can be completed in one closed tube at 39℃ within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type(WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay. Results The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units(pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay. Conclusion A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype.

关键词： Tick‐borne encephalitis virus Subtype Far‐eastern Detection RT‐RAA

TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus

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Biomedical and Environmental Sciences 2018年第3期31卷 208-214页

作者： SHAO Nan LI Fan NIE Kai FU Shi Hong ZHANG Wei Jia HE Ying lei wen wen WANG Qian Ying LIANG Guo Dong CAO Yu Xi WANG Huan Yu Department of Viral Encephalitis National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China State Key Laboratory of Infectious Disease Prevention and Control Beijing 102206 China State key laboratory for genetic engineering and molecular virology National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206China School of Public Health Shandong University Jinan 250012 Shandong China Office of laboratory management National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective To detect Japanese encephalitis virus（JEV） rapidly and distinguish its genotypes, a TaqMan-based reverse transcriptase quantitative polymerase chain reaction（RT-PCR） detection system was *** By aligning the full-length sequences of JEV（G1-G5）, six sets of highly specific TaqMan real-time RT-PCR primers and probes were designed based on the highly conserved NS1, NS2, and M genes of JEV, which included one set for non-specific JEV detection and five sets for the detection of specific JEV genotypes. Twenty batches of mosquito samples were used to evaluate our quantitative PCR *** With the specific assay, no other flavivirus were detected. The lower limits of detection of the system were 1 pfu/mL for JEV titers and 100 RNA copies/μL. The coefficients of variation of this real-time RT-PCR were all 〈 2.8%. The amplification efficiency of this method was between 90% and 103%.Conclusion A TaqMan real-time RT-PCR detection system was successfully established to detect and differentiate all five JEV genotypes.

关键词： Japanese encephalitis virus Genotype TaqMan real-time RT-PCR

Serological Survey of Zika Virus in Humans and Animals in Dejiang Prefecture, Guizhou Province,China

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Biomedical and Environmental Sciences 2019年第12期32卷 875-880页

作者： LI Fan ZHOU Jing Zhu ZHOU lei FU Shi Hong TIAN Zhen Zao WANG Qi SHAO Nan LI Dan HE Ying lei wen wen TANG Guang Peng LIANG Guo Dong WANG Ding Ming ZHANG Yan Ping WANG Huan Yu NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and PreventionBeijing 102206China Guizhou Center for Disease Control and Prevention Guiyang 550004China Public Health Emergency Center Chinese Center for Disease Control and PreventionBeijing 102206China

Objective The current outbreak of Zika virus(ZIKV)poses a severe threat to human *** ZIKV strains were isolated from mosquitoes collected from the Dejiang prefecture in China in 2016,which was the first isolation of ZIKV in nature in *** In this study,serum samples were collected from 366 healthy individuals and 104 animals from Dejiang prefecture in 2017,and the plaque reduction neutralization test(PRNT)was used to evaluate the seroprevalence of *** None of the 366 residents from whom the samples were collected were seropositive for *** of the 11 pigs from whom the samples were collected were seropositive for ZIKV,while 1 of 63(1.59%)chickens and 2 of 30(6.67%)sheep were seropositive for *** The extremely low seropositivity rate of ZIKV antibodies in animals in the Dejiang prefecture,Guizhou province in this study indicates that ZIKV can infect animals;however,there is a low risk of ZIKV circulating in the local population.

关键词： Zika Virus Serological survey Humans Animals China Plague reduction neutralization test

Development of an Internally Controlled Reverse Transcription Recombinase-aided Amplification Assay for the Rapid and Visual Detection of West Nile Virus

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Biomedical and Environmental Sciences 2019年第12期32卷 926-929页

作者： FAN Guo Hao SHEN Xin Xin LI Fan LI Xin Na BAI Xue Ding ZHANG Rui Qing WANG Rui Huan lei wen wen WANG Huan Yu MA Xue Jun WU Gui Zhen Department of Core Facility Ministry of Health Key Laboratory of Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Biosafety 3 Laboratory Chinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory of Infectious Disease Prevention and Control Chinese Center for Disease Control and PreventionBeijing 102206China Department of Viral Encephalitis NHC Key Laboratory of BiosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan 430071China

West Nile virus(WNV)causes West Nile fever and West Nile *** infection by WNV creates serious public health problems,its simple,rapid,and visual detection is very important in clinical practice,especially in resource-limited *** have developed a rapid,specific,and highly sensitive internally controlled reverse transcription recombinase-aided amplification(RTRAA)assay to detect WNV,using both real-timefluoresce nee and the lateral flow dipstick(LFD)at39.0°C for 30 *** analytical sensitivity of theRT-RAA assay was 10 plasmid copies and 1.6 pfu perreacti on with real-time fluoresce nee,and 1,000plasmid copies per reaction with the *** crossreactionwith other control viruses was *** with the RT-qPCR assay,the RT-RAA assaydemonstrated 100%sensitivity and 100%specificityfor WNV.

关键词： Internal Visual aided

柴油机活塞二阶运动对内冷油腔机油振荡流动与传热的影响

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农业工程学报 2017年第14期33卷 85-92页

作者：邓晰文雷基林文均温志高贾德文昆明理工大学大学云南省内燃机重点实验室昆明650500 成都银河动力有限公司成都610505

柴油机活塞的二阶运动不仅影响活塞侧击力、摩擦磨损、机油耗和漏气量,而且还对活塞内冷油腔内机油的振荡流动与传热性能产生影响。在活塞动力学与运动学分析的基础上,结合活塞内冷油腔内的振荡传热性能模拟试验结果,采用计算流体力学... 详细信息

柴油机活塞的二阶运动不仅影响活塞侧击力、摩擦磨损、机油耗和漏气量,而且还对活塞内冷油腔内机油的振荡流动与传热性能产生影响。在活塞动力学与运动学分析的基础上,结合活塞内冷油腔内的振荡传热性能模拟试验结果,采用计算流体力学仿真方法,建立了包含往复运动与二阶运动的计算流体力学仿真模型,研究了活塞二阶运动对内冷油腔内机油的振荡流动与传热性能的影响规律。研究结果发现,二阶运动的径向运动主要影响内冷油腔中机油的振荡流动,偏摆运动主要影响内冷油腔的瞬时换热性能。二阶运动使内冷油腔的瞬时充油率降低,循环平均降低4.6%。对油腔壁面的瞬时换热性能影响很大,最大的变化幅值为24.9%。对于整个换热过程,虽然充油率降低,但平均换热系数变化不大。因此,二阶运动对内冷油腔综合换热性能的影响可以忽略不计。该研究可为耐高温高强度铝合金活塞的设计提供理论和技术参考。

关键词：柴油机活塞传热二阶运动内冷油腔流动

Construction and Co-expression of Bicistronic Plasmid Encoding Human WEE1 and Stem Cell Factor

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Acta Biochimica et Biophysica Sinica 2005年第2期37卷 107-112页

作者： Ping lei wen-Han LI wen-Jun LIAO Bing YU Hui-Fen ZHU Jing-Fang SHAO Guan-Xin SHEN Laboratory of Molecular and Immuno-Pharmacology,Department of Immunology and Department of Pharmacology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China LaboratoryofMolecularandImmuno-Pharmacology DepartmentofImmunologyandDepartmentofPharmacologyTongjiMedicalCo

To protect the hematopoietic stem cells(HSCs)from apoptosis induced by chemotherapy andpromote HSC proliferation,bi-functional gene delivery systems are increasingly investigated in gene *** the present study,we constructed a bicistronic vector,pWISG,expressing the anti-apoptotic proteinhuman WEE1(WEE1Hu)and the fusion protein of the proliferation-stimulating stem cell factor(SCF)andenhanced green fluorescent protein(EGFP)separately with internal ribosome entry site(IRES).We firstexamined

关键词： internal ribosome entry site (IRES) human WEE1 (WEE1Hu) stem cell factor (SCF)

The potential mechanism and clinical application value of remote ischemic conditioning in stroke

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Neural Regeneration Research 2025年第6期20卷 1613-1627页

作者： Yajun Zhu Xiaoguo Li Xingwei lei Liuyang Tang Daochen wen Bo Zeng Xiaofeng Zhang Zichao Huang Zongduo Guo The First Affiliated Hospital of Chongqing Medical University ChongqingChina

Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.

关键词： Akt apoptosis autophagy cerebral perfusion cerebral vascular stenosis clinical transformation hemorrhagic stroke ischemic stroke neuroinflammation neuroprotection Notch1 PI3K remote ischemic conditioning stroke

韩剧中的悲情艺术——解读电影《婚纱》的修辞策略

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电影评介 2010年第10期 46-46,48页

作者：雷文文暨南大学华文学院

影片《婚纱》讲述的是一个凄美的亲情故事,导演充分利用了象征、反复、对比的修辞策略和表现手法,在一幅幅唯美的画面和一曲曲轻柔的音乐中带给观众最大的触动。悲情艺术是韩剧中经常使用的策略,这种诗化了的艺术正是对生活的感悟和适... 详细信息

影片《婚纱》讲述的是一个凄美的亲情故事,导演充分利用了象征、反复、对比的修辞策略和表现手法,在一幅幅唯美的画面和一曲曲轻柔的音乐中带给观众最大的触动。悲情艺术是韩剧中经常使用的策略,这种诗化了的艺术正是对生活的感悟和适度夸张。

关键词：韩剧亲情修辞策略悲情艺术

Identification of a Newly Isolated Getah Virus in the China-Laos Border,China

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Biomedical and Environmental Sciences 2017年第3期30卷 210-214页

作者： LI Yuan Yuan FU Shi Hong GUO Xiao Fang lei wen wen LI Xiao Long SONG Jing Dong CAO lei GAO Xiao Yan LYU Zhi HE Ying WANG Huan Yu REN Xiao Jie ZHOU Hong Ning WANG Gui Qin LIANG Guo Dong Shanxi Medical University Taiyuan 030001ShanxiChina State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310058ZhejiangChina Yunnan Institute of Parasitic Diseases Pu'er 665000YunnanChina

In this study, we isolated a virus strain （YN12031） from specimens of Armigeres subalbatus collected in the China-Laos border. BHK-21 cells infected with YN12031 exhibited an evident cytopathic effect （CPE） 32 h post-infection. The virus particles were spherical, 70 nm in diameter, and enveloped; they also featured surface fibers.

关键词： Getah virus China-Laos border Phenotypic characteristics Molecular evolution