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Biosynthesis of raw starch degradingβ-cyclodextrin glycosyltransferase by immobilized cells of Bacillus licheniformis using potato wastewater

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BiOCELL 2021年第6期45卷 1661-1672页

作者： YASSER S.MOSTAFA SAAD A.ALAMRi SULiMAN A.ALRUMMAN TAREK H.TAHA MOHAMED HASHEM MAHMOUD MOUSTAFA lamiaa i.fahmy Department of Biology College of ScienceKing Khalid UniversityAbha9004Saudi Arabia Prince Sultan Bin Abdulaziz Center for Environmental and Tourism Research and Studies King Khalid UniversityAbha9004Saudi Arabia Environmental Biotechnology Department Genetic Engineering and Biotechnology Research InstituteCity of Scientific Research&Technological ApplicationsNew Borg El-Arab City21934Egypt Department of Botany and Microbiology Faculty of ScienceAssiut UniversityAssiut71515Egypt Department of Botany and Microbiology Faculty of ScienceSouth Valley UniversityQena83523Egypt Department of Microbiology and immunology Faculty of PharmacyOctober University for Modern Science and ArtGiza12451Egypta

The study was sought to enhance the synthesis of thermal stableβ-cyclodextrin glycosyltransferase(β-CGTase)using potato wastewater as a low-cost medium and assess the degree to which it is efficient for industrial production ofβ-cyclodextrin(β-CD)from raw potato *** bacteria producingβ-CGTase was isolated from Saudi Arabia and the promising strain was identified as Bacillus licheniformis using phylogenetic analysis of the 16S rRNA ***-encapsulated cultures exhibited twice-fold ofβ-CGTase production more than free *** electron microscopy(SEM)of polymeric capsules indicated the potential for a longer shelf-life,which promotes the restoration of activity in bacterial cells across semi-continuous fermentation ofβ-CGTase production for 252 *** optimal conditions forβ-CGTase synthesis using potato wastewater medium were at 36 h,pH of 8.0,and 50°C with 0.4%potato starch and 0.6%yeast extract as carbon and nitrogen sources,*** purified enzyme showed a specific activity of 63.90 U/mg with a molecular weight of∼84.6 kDa as determined by SDS-PAGE *** high enzyme activity was observed up to 60°C,and complete stability was achieved at 75°*** levels of activity and stability were shown at pH 8.0,and the pH range from 7.0–10.0,*** enzyme has an appreciable affinity for raw potato starch with a Km of 5.7×10−6 M and a Vmax of 87.71μmoL/mL/min.β-CD production was effective against 25 U/g of raw potato *** outcomes demonstrated its feasibility to develop a fermentation process by integrating the cost-effective production ofβ-CGTase having distinctive properties forβ-CD production with ecofriendly utilization of potato wastewater.

关键词： Potato wastewater Bacillus licheniformis β-CGTase 16S rDNA gene Semi-continuous fermentation β-cyclodextrin

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