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砧木对元帅和金冠苹果25年中产量、产量效率和树体生长的影响

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国外农学（果树） 1992年第3期 1-4页

作者： F.e.larsen 董启凤

本文评价了9种砧木对金矮生(Gs)、好矮生(Ws)和8种砧木对红王(RK)、金冠(GD)苹果生长和产量的影响。短枝型品种(Ws和Gs),以实生砧、MM104和MM109为砧的树体最大,以M26作砧的树体最小。多数的组合年产量表现为平稳且25年后未显示明显下降... 详细信息

本文评价了9种砧木对金矮生(Gs)、好矮生(Ws)和8种砧木对红王(RK)、金冠(GD)苹果生长和产量的影响。短枝型品种(Ws和Gs),以实生砧、MM104和MM109为砧的树体最大,以M26作砧的树体最小。多数的组合年产量表现为平稳且25年后未显示明显下降。Ws的累积产量不明显高于RK(非短枝型)。GD的累积产量大于Gs。比较短枝型品种9种砧木组合的累积产量,以Gs/MM106和MM104及Ws/MM109和MM106组合最高。而产量效率以M26砧、短枝型品种和无性系砧木较高。

关键词：元帅苹果金冠苹果产量生长砧木

A new approach for the treatment of CLL using chlorambucil/hydroxychloroquine-loaded anti-CD20 nanoparticles

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Nano Research 2016年第2期9卷 537-548页

作者： sara Capolla Nelly Mezzaroba sonia Zorzet Claudio Tripodo Ramiro Mendoza-Maldonado Marilena Granzotto Francesca Vita Ruben spretz Gustavo larsen sandra Noriega eduardo Mansilla Michele Dal Bo Valter Gattei Gabriele Pozzato Luis Nunez Paolo Macor Department of Life sciences University of Trieste 34127 Trieste Italy Department of Human Pathology University of Palermo 90133 Sicilia Italy Molecular Oncology Unit National Laboratory Consorzio Interuniversitatio per le Biotecnologie (CIB) 34012 Trieste Italy Dipartimento Universitario Clinico di scienze mediche Chirurgiche e della Salute University of Trieste 34149 Trieste Italy LNK Chemsolutions LLC Lincoln NE 68521 USA Bio-Target Inc. Chicago IL 60637 USA Centro Unico Coordinador de Ablacion e Implante Provincia de Buenos Aires (C.U.CA.I.B.A.) Ministry of Health La Plata Buenos AiresArgentina Clinical and experimental Onco-Hematology Unit Centro di Riferimento Oncologico Istituto di Ricerca e Cura a Carattere Scientifico(I.R.C.C.S.) 33081 Aviano Italy Callerio Foundation Onlus Institutes of Biological Researches 34127 Trieste Italy

Current approaches for the treatment of chronic lymphocytic leukemia （CLL） have greatly improved the prognosis for survival, but some patients remain refractive to these therapeutic regimens. Hence, in addition to reducing the long-term side- effects of therapeutics for all leukemia patients, there is an urgent need for novel therapeutic strategies for difficult-to-treat leukemia cases. Due to the cytotoxicity of drugs, the major challenge currently is to deliver the therapeutic agents to neoplastic cells while preserving the viability of non-malignant ceils. In this study, we propose a therapeutic approach in which high doses of hydroxychloroquine and chlorambucil were loaded into biodegradable polymeric nanopartides coated with an anti-CD20 *** first demonstrated the ability of the nanoparticles to target and internalize in tumor B-cells. Moreover, these nanoparticles could kill not only p53-mutated]deleted leukemia cells expressing a low amount of CD20, but also circulating primary ceils isolated from chronic lymphocytic leukemia patients. The safety of these nanoparticles was also demonstrated in healthy mice, and their therapeutic effects were shown in a new model of aggressive leukemia. These results showed that anti-CD20 nanoparticles containing hydroxychloroquine and chlorambucil can be effective in controlling aggressive leukemia and provided a rationale for adopting this approach for the treatment of other B-cell disorders.

关键词： chronic lymphocytic leukemia immune targeted nanoparticles treatment xenograft model

Tailoring Properties of Biocompatible PeG-DMA Hydrogels with UV Light

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Materials sciences and Applications 2012年第6期3卷 425-431页

作者： sania Backstrom Juana Benavente Rolf W.Berg Karin stibius Marianne s.larsen Henrik Bohr Claus Hélix-Nielsen The Biomimetic Membrane Group DTU PhysicsTechnical University of DenmarkLyngbyDenmark Aquaporin A/s Ole Maaloes Vej 3CopenhagenDenmark Grupo de Caracterización electrocinética en Membranas e Interfases Departamento de Física Aplicada IFacultad de CienciasUniversidad de MálagaMálagaSpain DTU Chemistry Technical University of DenmarkLyngbyDenmark Quantum Protein Center DTU PhysicsTechnical University of DenmarkLyngbyDenmark

Hydrogels are highly water-absorbent hydrophilic polymer networks, which show potential in many biocompatible applications. In previous work, we demonstrated the feasibility of using poly(ethylene glycol) dimethacrylate (PeG-DMA) gels polymerized with a photoinitiator for encapsulation and stabilization of painted biomimetic membrane arrays for novel separation technologies or biosensor applications. These gels were formed from PeG-DMA monomers suspended in phosphate buffered saline (PBs) solution and gelated by radical polymerization in the presence of the photoinitiator Darocur 1173. In this work, we show that the properties of a PeG-DMA hydrogel formed by photoinitiated polymerizetion can be tailored by varying the photocrosslinking time. Fourier Transform Infrared spectroscopy (FTIR) and Raman spectroscopy (Rs) showed that the optimal crosslinking time for the gel was 6 - 10 minutes and that the water content of the gels could be tuned in the range of 50 - 90 wt%. The resistivity was between 0.8 - 3.5 Ωm, which is comparable to that of PBs. The low resistivity of the gel makes it compatible for encapsulating membranes for (ion channel based) biosensor applications. With FTIR and Rs we identified spectral features of the hydrogel, which may serve as a diagnostic tool to monitor changes in the gels due to variation in parameters such as time, pH, temperature, aging or exposure to chemicals or biological material.

关键词： Hydrogel swelling UV-Induced Polymerization FTIR Raman spectroscopy Impedance spectroscopy

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吡喹酮抗犬细粒棘球绦虫的效果

中国兽医科学 1981年第1期 60-61页

作者：李明忠 R.e.larsen 四川农学院畜牧兽医系

由于缺乏一种对犬细粒棘球绦虫的有效驱虫药,使包虫病的防治未曾取得应有的进展。一种对未成熟的和成熟的细粒棘球绦虫都能驱杀的药物,并且具有杀灭虫卵的效力,是用于治疗和防治的理想药物。泛美兽毒感染病研究中心,为了发展防治计划,... 详细信息

由于缺乏一种对犬细粒棘球绦虫的有效驱虫药,使包虫病的防治未曾取得应有的进展。一种对未成熟的和成熟的细粒棘球绦虫都能驱杀的药物,并且具有杀灭虫卵的效力,是用于治疗和防治的理想药物。泛美兽毒感染病研究中心,为了发展防治计划,几年来开展了抗细粒棘球绦虫药物的研究。包虫病是地方流行病。此病在阿根廷、智利、乌拉圭、秘鲁的sierra和巴西的Rio Grandedosul州;造成严重的经济损失。所以在这些国家的一些地方开始执行防治计划,决定研究南美洲宿主与寄生虫的关系,研究吡喹酮抗犬的细粒棘球绦虫的效果,以决定它能否被推荐

关键词：细粒棘球绦虫药物未成熟犬绦虫防治计划寄生虫寄生物

enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease

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Open Journal of Animal sciences 2017年第3期7卷 241-253页

作者： Alejandra larsen santiago Corva Javier Panei Christoph Geisler eduardo Mortola Faculty of Veterinary science National University of La Plata La Plata Argentina CONICeT (CCT-La Plata) La Plata Argentina Department of Immunology Faculty of Veterinary Sciences National University of La Plata La Plata Argentina d Department of Molecular Biology University of Wyoming Laramie WY USA

enzyme-linked immunosorbent assay (eLIsA) is often used to test bovine leukemia virus (BLV) infection. However, commercially available kits test in south America detect only antibodies against the gp51 protein. With the aim to improve the sensitivity of the test, we developed here a two-step indirect dual eLIsA test that included both proteins p24 and gp51, expressed and produced in e. coli and baculovirus expression system respectively. Two hundred ten BLV sera, stated as double positive or double negative by the combination of commercial agar gel immunodiffusion (AGID) assay and a gp51-eLIsA test, were tested with our in house dual rp24/rgp51 eLIsA. Firstly, we checked the purified, optimized and standardized proteins as antigen by the checkerboard technique, and set up our in house eLIsA test. The concordance correlation coefficient (CCC) and coefficient of variation (CV) intraplate repeatability levels were within the values established by the international standards. The statistical analysis demonstrated the value of sera correctly ranked highest (93.48%), and for 0.3 cutoff, the sensitivity was 95.65% and the specificity 91.30%. In conclusion, the rp24/rgp51 eLIsA developed and standardized here demonstrated to have good analytical characteristics to be considered for screening of BLV.

关键词： Bovine Leukemia Virus Indirect Dual eLIsA Recombinant Antigens

Wastewater surveillance provides 10-days forecasting of COVID-19 hospitalizations superior to cases and test positivity:A prediction study

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Infectious Disease Modelling 2023年第4期8卷 1138-1150页

作者： Dustin T.Hill Mohammed A.Alazawi e.Joe Moran Lydia J.Bennett Ian Bradley Mary B.Collins Christopher J.Gobler Hyatt Green Tabassum Z.Insaf Brittany Kmush Dana Neigel shailla Raymond Mian Wang Yinyin Ye David A.larsen Department of Public Health Syracuse UniversitySyracuseNY13244USA Center for environmental Health New York State Department of HealthAlbanyNYUSA CDC Foundation AtlantaGAUSA Department of Civil Structural and Environmental EngineeringUniversity at BuffaloBuffaloNYUSA school of Marine and Atmospheric sciences Sustainability Studies DivisionStony Brook UniversityStony BrookNYUSA Institute for Advanced Computational science Stony Brook UniversityStony BrookNYUSA New York state Center for Clean Water Technology Stony Brook UniversityStony BrookNYUSA school of Marine and Atmospheric sciences Stony Brook UniversityStony BrookNYUSA Department of environmental Biology State University of New York College of Environmental Science and ForestrySyracuseNYUSA Department of epidemiology and Biostatistics School of Public HealthUniversity at AlbanyRensselaerNYUSA Department of Civil engineering College of Engineering and Applied SciencesStony Brook UniversityStony BrookNYUSA

Background:The public health response to COVID-19 has shifted to reducing deaths and hospitalizations to prevent overwhelming health *** amount of sARs-CoV-2 RNA fragments in wastewater are known to correlate with clinical data including cases and hospital admissions for *** developed and tested a predictive model for incident COVID-19 hospital admissions in New York state using wastewater ***:Using county-level COVID-19 hospital admissions and wastewater surveillance covering 13.8 million people across 56 counties,we fit a generalized linear mixed model predicting new hospital admissions from wastewater concentrations of sARs-CoV-2 RNA from April 29,2020 to June 30,*** included covariates such as COVID-19 vaccine coverage in the county,comorbidities,demographic variables,and holiday ***:Wastewater concentrations of sARs-CoV-2 RNA correlated with new hospital admissions per 100,000 up to ten days prior to *** that included wastewater had higher predictive power than models that included clinical cases only,increasing the accuracy of the model by 15%.Predicted hospital admissions correlated highly with observed admissions(r¼0.77)with an average difference of 0.013 hospitalizations per 100,000(95%CI¼[0.002,0.025])Interpretation:Using wastewater to predict future hospital admissions from COVID-19 is accurate and effective with superior results to using case data *** lead time of ten days could alert the public to take precautions and improve resource allocation for seasonal surges.

关键词： COVID-19 hospitalizations Wastewater-based epidemiology Forecasting Prediction sARs-CoV-2

分子吸附再循环系统对肝性脑病患者动脉氨基酸水平及大脑氨基酸代谢的影响

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世界核心医学期刊文摘（胃肠病学分册） 2005年第3期1卷 61-62页

作者： schmidt L.e. Tofteng F. strauss G.I. larsen F.s. 程欣 Dept. of Hepatology A. 2.12.1 Rigshospitalet Blegdamsvej 9 DK-2100 CopenhagenФ Denmark

Background: Liver failure is associated with low concentrations of branched chain amino acids and high concentrations of most other amino acids. In this study the effect of treatment with the Molecular Adsorbents Recirculating system (MARs) on arterial amino acid levels and cerebral amino acid metabolism was examined in patients with severe hepatic encephalopathy. Methods: The study included seven patients with hepatic encephalopathy from fulminant hepatic failure (FHF) and five patients with hepatic encephalopathy from acute on chronic liver failure (AoCLF). Cerebral blood flow and cerebral arteriovenous differences in amino acids were measured before and after 6 h of treatment with MARs. Results: During MARs treatment, the total arterial amino acid concentration decreased by 20%from 8.92 ±7.79 mmol/L to 7.16 ±5.64 mmol/L (P e concentration decreased in all amino acids with the exception of the branched chain amino acids. Fischer’s ratio of branched chain to aromatic amino acids increased from 0.73 ±0.47 to 0.91 ±0.54 (P et cerebral efflux of amino acids in patients with FHF (8.94 ±8.34 μmol;/100 g/min) as well as AoCLF (7.35 ±24.97 μmol/100 g/min) was not affected by the MARs treatment. MARs had no effect on the cerebral metabolic rate of any single amino acid in either group. Conclusions: MARs treatment tends to normalize the arterial amino acid concentrations in patients with hepatic encephalopathy. even though the overall reduction in plasma amino acids and improvement in amino acid dysbalance may well be beneficial, it was not accompanied by any immediate improvement in cerebral amino acid metabolism in patients with FHF or AoCLF.

关键词：肝性脑病氨基酸水平氨基酸代谢暴发性肝衰竭支链氨基酸血流量慢性肝衰竭动静脉急性发作 Fischer

超声内镜引导细针穿刺和支气管内超声引导的经支气管针吸:在纵隔病变中的联合检测

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世界核心医学期刊文摘（胃肠病学分册） 2006年第1期2卷 29-30页

作者： Vilmann P. Krasnik M. larsen s.s. 赵萌 Dept. of surgical Gastroenterology D Gentofte University Hospital Niels Andersensvej 65 2900 Hellerup Denmark Dr.

Background and study aims: It would be desirable to develop minimally invasive methods of tissue diagnosis from lymph nodes as well as solid lesions in the mediastinum. The aim of the present study was to test the combined method of transesophageal endoscopic ultrasound-guided fine-needle aspiration (eUs-FNA)and endobronchial ultrasound-guided transbronchial needle aspiration (eBUs-TBNA) in the evaluation of mediastinal lesions. Patients and methods: eUs-FNA and eBUs-TBNA were compared in 33 patients, for the staging of lung cancer in patients with an established diagnosis of nonsmall-cell lung cancer (n = 20) or for diagnosis of a suspicious mediastinal lesion in patients with suspected lung cancer (n = 13). e-BUs-TBNA and eUs-FNA were unsuccessful in one patient each. The diagnoses were verified in 28 of the remaining 31 patients either at thoracotomy (n = 9) or during the clinical follow-up (n = 19). Results: A total of 119 lesions were sampled by eUs-FNA (n = 59) and eBUs-TBNA (n = 60). eUs-FNA and eBUs-TBNA demonstrated cancer in 26 and 28 lesions, respectively, and benign cytology in 30 and 28 lesions, respectively. suspicious cells were found in three and four lesions by eUs-FNA and eBUs-TBNA, respectively. When the 60 eBUs-TBNA samples were compared with the 59 eUs-FNA samples, 11 additional cancer diagnoses and three samples with suspicious cells were obtained by eBUs-TBNA that had not been obtained by eUs-FNA. Conversely, eUsFNA diagnosed 12 additional cancer diagnoses, one suspicious and one specific benign diagnosis (sarcoidosis) in addition to *** a combined approach (eUs-FNA+ eBUs-TBNA) in 28 of the 31 patients in whom a final diagnosis was obtained in the evaluation of mediastinal cancer, 20 patients were found to have mediastinal involvement, whereas no mediastinal metastases were found in eight patients. The accuracy of eUs-FNA and eBUs-TBNA, in combination, for the diagnosis of mediastinal cancer was 100% (95% CI, 83-100% ). Conclusions:

关键词：经支气管针吸纵隔病变超声内镜支气管内细针穿刺非小细胞肺癌临床诊断病变诊断微创性良性

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克罗恩病的小肠磁共振成像

世界核心医学期刊文摘（胃肠病学分册） 2005年第12期1卷 53-54页

作者： Frφkjr J.B. larsen e. steffensen e. 樊菁 Centre for Visceral Biomechanics and Pain Department of Gastroenterology Aalborg Hospital DK- 9000 Aalborg Denmark Dr.

Objective. There may be advantages in using magnetic resonance imaging (MRI) in small- bowel disease. The aims of this study were to optimize the MRI examination technique and to evaluate the capabilities of MRI compared with those of conventional enteroclysis (Ce). Material and methods. MRI and Ce were performed in 36 patients suspected of Crohn’ s disease. Based on 26 pilot studies optimal oral administration of plum juice and bulk fibre laxative was found. T2- weighted and gadolinium enhanced T1- weighted images were obtained using a breath- holding technique and butylscopolamine. Virtual endoscopy was performed. Conventional enteroclysis entailed duodenal intubation and administration of barium and air. Two radiologists evaluated the examinations independently. Finally, each patient scored the degree of discomfort, and preference for either MRI or Ce was found. Results. The MRI technique ensured sufficient distension of the small bowel and small- bowel changes were found in 12 patients. In 3 patients this was not seen on conventional enteroclysis, which did not reveal any pathology that was not already seen on MRI. Pathological abdominal changes were found in 70% more patients during MRI than during conventional enteroclysis (p endoscopic examination corresponded with the MRI findings. The examination quality decreased with increasing age (p = 0.002) and the interobserver agreement of the pathological changes was high (p endoscopy resulted in excellent demonstration of the mucosal surface. The examination discomfort scores obtained during the MRI were lower than those during conventional enteroclysis (psions. MRI using the current technique is preferable to conventional enteroclysis because of superior demonstration of the entire small- bowel pathology, low level of patient discomfort and absence of radiation exposure.

关键词：克罗恩病小肠疾病磁共振成像加权像肠造影不适程度缓泻剂显示能力影像学医师膨胀度