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同济大学学报（自然科学版） 2014年第2期42卷 272-277页

作者：田富箱徐斌秦朗张天阳同济大学污染控制与资源化研究国家重点实验室上海200092 长江水环境教育部重点实验室上海200092

研究了不同pH下,投加不同浓度的外加溴离子时,NDMA前体物—Chlortoluron的氯化动力学降解情况.实验结果表明,在pH分别为6,7,10,Chlortoluron浓度为9.4μmol·l-1,Cl2浓度为188.0μmol·l-1的条件下,控制投加不同浓度的溴离子时,Chlortol... 详细信息

研究了不同pH下,投加不同浓度的外加溴离子时,NDMA前体物—Chlortoluron的氯化动力学降解情况.实验结果表明,在pH分别为6,7,10,Chlortoluron浓度为9.4μmol·l-1,Cl2浓度为188.0μmol·l-1的条件下,控制投加不同浓度的溴离子时,Chlortoluron的降解仍然符合拟一级动力学模型.当pH分别为6,7时,Chlortoluron的降解速率随着外加溴离子浓度的增加而增大,外加溴离子浓度相同时,偏酸性的环境使Chlortoluron的降解速率更快,这可能与反应体系中HOBr的生成有关.当pH为10时,溴离子浓度的变化对Chlortoluron的降解速率没有显著影响,可能是ClO-与Br-基本不发生反应,从而没有生成HOBr.根据对反应体系的动力学分析,求解了在本实验条件下,不同pH时拟一级反应动力学常数kobs与[Br2]/[Cl2]的线性关系,进而通过求解常微分方程组计算了HOBr与Chlortoluron的反应速率常数(k5=(1.95±0.44)×105).这也从动力学的角度解释了外加溴离子时Chlortoluron氯化速率显著增大的原因.

关键词：溴离子氯化 NDMA 前体物 Chlortoluron

Development of a sensitive and reliable reverse transcription droplet digital PCR assay for the detection of Citrus tristeza virus

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Development of a sensitive and reliable reverse transcriptio...

中国植物病理学会2019年学术年会

作者： Wang Yingli Wang qin Yang Zhen li Ruhui liu Yingjie li Jifen li Zhengwen Zhou Yan National Citrus Engineering Research Center Citrus Research InstituteSouthwest University Academy of Agricultural Sciences Southwest University USDA-ARS National Germplasm Resources Laboratory Xinping Yi-Dai Autonomous County Agricultural Bureau

Citrus tristeza virus(CTV) is one of the most important viruses infecting citrus in the *** digital PCR(ddPCR) is a novel sensitive,accurate method that enables absolute quantitation without the need for a standard *** aim of this study was to develop a reverse transcription droplet digital PCR(RT-ddPCR) method to detect CTV,and to compare its quantitative linearity,sensitivity and accuracy with *** results indicated that both methods showed a high degree of linearity(R=0.991) and quantitative *** assays suggested that the detection limit for RT-ddPCR was approximately 4.0×10 copies/μl,a 100-fold greater sensitivity than *** detection results for heat-treatment citrus samples also showed that the positive detection rate of RTddPCR(73.2%) was higher than that of RT-qPCR(53.6%).In summary,the results demonstrated that RT-ddPCR may contribute to improve CTV diagnosis by its higher sensitivity and specificity.

关键词： RT-ddPCR CTV detection

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考虑广告主投资竞争的关键词拍卖研究

中国管理科学 2019年第5期27卷 184-195页

作者：杨琴黄河王宗军华中科技大学管理学院湖北武汉430074 重庆大学经济与工商管理学院重庆400044

关键词拍卖是搜索引擎盈利手段之一,同时给广告主带来高额回报。在搜索引擎注重质量权重的拍卖规则的推动下和广告主自身利益的驱使下,广告主通过投资来提高自身表现水平,赢得更好排位增加点击量。引入广告主投资,并用连续可变的努力水... 详细信息

关键词拍卖是搜索引擎盈利手段之一,同时给广告主带来高额回报。在搜索引擎注重质量权重的拍卖规则的推动下和广告主自身利益的驱使下,广告主通过投资来提高自身表现水平,赢得更好排位增加点击量。引入广告主投资,并用连续可变的努力水平来表征参与竞价的广告主投资过程中所付出的各种要素和资源投入,考虑投资和竞价两阶段模型,分析高低两类广告主的努力水平决策及均衡竞价策略。研究表明当满足初始投入最低努力水平时的边际成本大于边际收益的条件时,决定低类型广告主类型转换的估价阈值存在且唯一,并且在情形一中,潜力广告主的最优努力水平总是大于高类型广告主,在情形二中,随着估价的增大,两类广告主的最优努力水平趋于一致;同时,搜索引擎拍卖规则会影响广告主投资过程中最优努力水平的决策:搜索引擎给低类型广告主的质量权重越大,转换类型的估价阈值就越高,且潜力广告主取内点解时的最优努力水平和高类型广告主的最优努力水平均随之减少。最后,通过数值算例分析了两类广告主最优努力水平以及拍卖规则对广告主努力水平的影响。

关键词：拍卖投资竞争均衡竞价策略最优努力水平

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异双席夫碱配合物中性载体碘离子电极的研究

分析试验室 2009年第1期28卷 84-87页

作者：徐刚袁若柴雅琴西南大学化学化工学院重庆市现代分析化学重点实验室重庆科技学院

研究了以乙二胺、乙酰丙酮缩水杨醛异双席夫碱合钴（Ⅱ）为载体的PVC膜电极,该电极对I;具有优良的电位响应特性并呈现反Hofmeister选择性行为,其选择性次序为I;>Sal;>SCN;>ClO;>SO;>Br;>NO;>Cl;>Ac;>NO;>SO;。在pH 2.0的磷酸盐缓冲体系... 详细信息

研究了以乙二胺、乙酰丙酮缩水杨醛异双席夫碱合钴（Ⅱ）为载体的PVC膜电极,该电极对I;具有优良的电位响应特性并呈现反Hofmeister选择性行为,其选择性次序为I;>Sal;>SCN;>ClO;>SO;>Br;>NO;>Cl;>Ac;>NO;>SO;。在pH 2.0的磷酸盐缓冲体系中,对I;在1.0×10;～1.0×10;mol/l浓度范围内呈近能斯特响应,斜率为-50.7 mV/dec（26℃）,检出限为3.9×10;mol/l。采用交流阻抗技术和紫外可见光谱技术研究了电极的响应机理,结果表明配合物中心金属原子的结构以及栽体本身的结构与电极的响应行为之间有非常密切的构效关系。将电极初步应用于药物和食盐中碘的分析。

关键词：异双席夫碱合钴(Ⅱ) 中性载体碘离子选择性电极

OBSERVATION OF THE ClINICAl EFFECTS OF ElECTROACUPUNCTURE COMPOSITE REHABIlITATION TRAINING ON THE MOTION FUNCTION OF PATIENTS WITH INCOMPlETE SPINAl CORD INJURY

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Journal of Integrative Medicine 2014年第3期 270-271页

作者： Hui-hui qin An-ren Zhang Wen-chun Wang Ri-zhao Pang Bei li Xiao-hua Ke General Hospital of Chengdu Military Region of PlA

Objective: To testify the effectiveness of electroacupuncture treatment by observing the improved situation of motion function in patients with incomplete spinal cord injury treated by elec troacupuncture composite rehabilitation training, and to provide a new method for the patients with incomplete spinal cord injury Methods: Randomized, double-blind and blank-contro method was ***-two patients with incomplete spina cord injury were assigned into the observation group, in which acupuncture with the electric pulse stimulation was applied to the motor points of the key muscles of the lower extremities, and the control group, in which electroacupuncture was applied a Biguan, Yinshi, Futu, liangqiu, Fenglong, Chongyang, and Jiexie In each group, treatment was given once a day; one-month treat ment made a *** sessions were required totally with two-day rest between each *** and after each session the American Spinal Injury Association(ASIA) impairmen scale, ASIA motor score, modified Barthel index(MBI), and integrated electromyography tests were adapted to the *** differences in the efficacy on the motion function were compared with the results before and after ***: The changes of ASIA impairment scale were not statisti cally different(P>0.05) at 1 h, 2 and 3 months after treatment While the changes of ASIA motor score, MBI, and integrated electromyography were statistically different when tested afte treatment for 1, 2 and 3 *** meant the motion function of the subjects was improved in both *** ASIA motoscore, MBI, and integrated electromyography changes were of no statistical significance(P>0.05) at 1 and 2 months after treatment, while were of statistical difference at 3 *** effect of the treatment group was ***: Both the electroacupuncture and traditional body acupuncture could improve the motion function of patients with incomplete spinal cord ***, concerning in the l

关键词： ASIA MBI OBSERVATION OF THE ClINICAl EFFECTS OF ElECTROACUPUNCTURE COMPOSITE REHABIlITATION TRAINING ON THE MOTION FUNCTION OF PATIENTS WITH INCOMPlETE SPINAl CORD INJURY

瑟南古的东方视野——《中国历史概述》中的华夏文明镜像

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华文文学 2017年第4期 29-34页

作者：陈沁钱林森南京大学巴黎索邦大学南京大学比较文学与比较文化研究所

《中国历史概述》是法国浪漫主义运动先驱瑟南古的重要作品。成书于欧洲汉学盛世尾声的过渡时期,这部中国历史著作上承中法关系盛世,下接近代历史战争年代,对欧洲汉学研究具有不可替代的文献价值与历史影响力。本文以《中国历史概述》... 详细信息

《中国历史概述》是法国浪漫主义运动先驱瑟南古的重要作品。成书于欧洲汉学盛世尾声的过渡时期,这部中国历史著作上承中法关系盛世,下接近代历史战争年代,对欧洲汉学研究具有不可替代的文献价值与历史影响力。本文以《中国历史概述》为考察对象,结合译文与相关史料,就作者的书写内容与意图、成书宗旨与论证方式、对中欧文化差异的关键词解读与西学东渐的传教策略做一探讨,力图解读中国形象"乌托邦化"与"去乌托邦化"历史博弈的语境下所呈现的"文化他者"形象。

关键词：瑟南古《中国历史概述》东方视野文化差异传教策略

龙胆酸对B-Z化学振荡反应的影响及其分析测定

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安徽大学学报（自然科学版） 2012年第3期36卷 79-84页

作者：王勤胡刚孟敏安徽大学化学化工学院安徽合肥230039

利用龙胆酸对四氮杂大环铜催化的B-Z化学振荡体系(NaBrO3-H2SO4-苹果酸-[Cul](ClO4)2)的扰动建立测定龙胆酸的新方法.其分析结果表明龙胆酸的浓度在1.25×10-6～1×10-4mol.l-1之间时,振幅改变值与加入龙胆酸的浓度对数有良好的线性关系... 详细信息

利用龙胆酸对四氮杂大环铜催化的B-Z化学振荡体系(NaBrO3-H2SO4-苹果酸-[Cul](ClO4)2)的扰动建立测定龙胆酸的新方法.其分析结果表明龙胆酸的浓度在1.25×10-6～1×10-4mol.l-1之间时,振幅改变值与加入龙胆酸的浓度对数有良好的线性关系,其相关系数为0.99821.同时对各组分浓度的影响及其扰动机理进行了探讨.

关键词：龙胆酸化学振荡反应杂大环配合物

A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)

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Chinese Science Bulletin 2003年第10期48卷 941-948页

作者： qin E'de ZHU qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao lIU Bohua DENG Yongqiang lIU Hong ZHANG Yu WANG Cui lI Yuquan GAN Yonghua lI Xiaoyu l Fushuang TAN Gang CAO Wuchun YANG Ruifu WANG Jian lI Wei XU Zuyuan lI Yan WU qingfa lIN Wei CHEN Weijun TANG lin DENG Yajun HAN Yujun lI Changfeng lEI Meng lI Guoqing lI Wenjie l Hong SHI Jianping TONG Zongzhong ZHANG Feng lI Songgang lIU Bin lIU Siqi DONG Wei WANG Jun Gane K-S Wong YU Jun YANG Huanming Institute of Microbiology and Epidemiology Chinese Academy of Military Medical Sciences Beijing 100071 China Beijing Genomics Institute Chinese Academy of Sciences Beijing 101300 National Center for Genome Information Beijing 101300 China

The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s).

关键词：比较分析 SARS 非典型肺炎冠状病毒病因学发病机理诊断学疫苗

应用二温式PCR检测家蚕核型多角体病毒的方法

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蚕业科学 2015年第3期41卷 565-570页

作者：覃玥陈保善河池学院化学与生物工程学院广西宜州546300 广西大学生命科学与技术学院南宁530004 广西亚热带生物资源保护利用重点实验室南宁530004

为了简便、快速、准确地检测家蚕核型多角体病毒(Bm NPV),以Bm NPV广西分离株的多角体蛋白基因(polh)序列(Gen Bank登录号:JQ991011.1)设计引物,以感染Bm NPV(广西宜州分离株)2 h的5龄家蚕幼虫中肠组织提取的DNA为模板,建立二温式PCR检... 详细信息

为了简便、快速、准确地检测家蚕核型多角体病毒(Bm NPV),以Bm NPV广西分离株的多角体蛋白基因(polh)序列(Gen Bank登录号:JQ991011.1)设计引物,以感染Bm NPV(广西宜州分离株)2 h的5龄家蚕幼虫中肠组织提取的DNA为模板,建立二温式PCR检测Bm NPV的方法,最终确定的最佳反应体系为:10×PCR buffer 1.5μl,5 mmol/l Mg Cl22μl,2.5mmol/l d NTP 0.5μl,10μmol/l上、下游引物各1μl,5 U/μl Taq DNA聚合酶0.1μl,18 ng/μl模板DNA 1μl,加水至总体积15μl。确定的最佳反应条件为:94℃预变性3 min;95℃15 s,延伸和退火温度合并为62℃30 s,共25个循环。按上述条件PCR扩增得到大小约309 bp的特异性片段,测序结果与已知polh基因序列的相似度为100%。与普通PCR检测方法比较,采用二温式PCR方法对样本的检测时间节省1.5 h,反应特异性强,灵敏度高(能被检测的最小感病组织基因组DNA的质量浓度为1.8 pg/μl),并且选择最初2 h感病的幼虫中肠组织为检测材料,因而可用于家蚕血液型脓病的早期诊断。

关键词：家蚕核型多角体病毒多角体蛋白基因二温式PCR 反应条件