检索结果-南通市图书馆

AMBIO－人类环境杂志 2004年第S1期33卷 39-49,57页

作者： Terry V.Callaghan M.Johansson O.W.Heal N.R.Slthun l.J.Barkved N.Bayfield O.Brandt R.Brooker H.H.Christiansen M.Forchhammer T.T.Hφye O.Humlum A.Jrvinen C.Jonasson J.Kohler B.Magnusson H.Meltofte l.mortensen S.Neuvonen I.Pearce M.Rasch l.Turner B.Hasholt E.Huhta E.leskinen N.Nielsen P.Siikamki 金博文 Abisko Scientific Research Station

北大西洋周边区域(SCANNET区域)涵盖了广泛的气候区、自然环境和可用自然资源范围。除了最北部地区,至少自最后一次冰期结束以来,这里就有人和多种文化存在。然而,该区在大的地理尺度上也是非常重要的,因为它影响着全球气候并支撑着在... 详细信息

北大西洋周边区域(SCANNET区域)涵盖了广泛的气候区、自然环境和可用自然资源范围。除了最北部地区,至少自最后一次冰期结束以来,这里就有人和多种文化存在。然而,该区在大的地理尺度上也是非常重要的,因为它影响着全球气候并支撑着在北极地区和世界其它大陆之间迁徙的动物。这一区域的气候、环境和土地利用正在发生急剧变化,预测显示,全球变暖在这里的表现会更强,而土地利用的增加会使剩余的野地急剧减少。因为本区大部分地方人口稀疏(如果有人居住的话),过去环境变化及其影响方面的观测记录也都是很少而且时段很短的。然而,记录现在正在进行的变化、认识这些变化的驱动力和预测这些变化的后果变得非常重要了。为了促进认识全球变化对北大西洋区域陆地的影响的研究,同时也为了实时监测这种变化,2000年欧共体资助成立了一个研究站点网络及其下设机构,命名为SCANNET——斯堪的纳维亚(半岛)(瑞典、挪威、丹麦、冰岛的泛称)/北欧陆地野外基础网络。SCANNET目前包括9个核心站及当地网络里面的5个站,它们一起覆盖了该区当前气候和预测的变化的广泛区域。气候指标在网络内部是共同的,而每个站点都选择特殊的环境和生态主题进行精细观测。这既保证了主题覆盖面的多样性又保证了专门技术的多样性。我们总结了SCANNET到目前为止的发现,并概述其资料基础,以增加对北大西洋环境变化数据的了解。同时我们也总结我们在理解方面的重要缺陷以及SCANNET已经存在机构和行动在促进今后的研究、监测和野外实证行动方面的作用。

关键词： SCANNET 全球变暖北部地区多年冻土斯瓦尔巴德群岛土地利用基础网络合作途径冻胀丘

Typing of pancreatic cancer-associated fibroblasts identifies different subpopulations

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第41期24卷 4663-4678页

作者： Michael Friberg Bruun Nielsen Michael Bau mortensen Sonke Detlefsen Department of Pathology Odense University HospitalDepartment of Clinical ResearchUniversity of Southern DenmarkOdense Pancreas Center(OPAC)Odense C 5000Denmark Department of Surgery HPB SectionOdense University HospitalDepartment of Clinical ResearchUniversity of Southern DenmarkOdense Pancreas Center(OPAC)Odense C 5000Denmark

AIM To determine whether it is possible to identify different immune phenotypic subpopulations of cancer-associated fibroblasts (CAFs) in pancreatic cancer (PC). METHODS We defined four different stromal compartments in surgical specimens with PC: The juxtatumoural, peripheral, lobular and septal stroma. Tissue microarrays were produced containing all pre-defined PC compartments, and the expression of 37 fibroblast (FB) and 8 extracellular matrix (ECM) markers was evaluated by immunohistochemistry, immunofluorescence (IF), double-IF, and/or in situ hybridization. The compartment-specific mean labelling score was determined for each marker using a four-tiered scoring system. DOG1 gene expression was examined byquantitative reverse transcription PCR (qPCR). RESUlTS CD10, CD271, cytoglobin, DOG1, miR-21, nestin, and tenascin C exhibited significant differences in expression profiles between the juxtatumoural and peripheral compartments. The expression of CD10, cytoglobin, DOG1, nestin, and miR-21 was moderate/strong in juxtatumoural CAFs (j-CAFs) and barely perceptible/weak in peripheral CAFs (p-CAFs). The upregulation of DOG1 gene expression in PC compared to normal pancreas was verified by qPCR. Tenascin C expression was strong in the juxtatumoural ECM and barely perceptible/weak in the peripheral ECM. CD271 expression was barely perceptible in j-CAFs but moderate in the other compartments. Galectin-1 was stronger expressed in j-CAFs vs septal fibroblasts, PDGFRβ, tissue transglutaminase 2, and hyaluronic acid were stronger expressed in lobular fibroblasts vs p-CAFs, and plectin-1 was stronger expressed in j-CAFs vs l-FBs. The expression of the remaining 33 markers did not differ significantly when related to the quantity of CAFs/FBs or the amount of ECM in the respective *** Different immune phenotypic CAF subpopulations can be identified in PC, using markers such as cytoglobin, CD271, and miR-21. Future studies should determine whether CAF subpo

关键词： Pancreatic cancer Tumour stroma Cancerassociated fibroblasts Extracellular matrix Subtyping Immunohistochemistry

Fur glowing under ultraviolet:in situ analysis of porphyrin accumulation in the skin appendages of mammals

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2023年第1期18卷 15-26页

作者： Séverine l.D.TOUSSAINT Jasper PONSTEIN Mathieu THOURY Rémi MÉTIVIER Daniela C.KAlTHOFF Benoît HABERMEYER Roger GUIlARD Steffen BOCK Peter mortensen Sverre SANDBERG Pierre GUERIAU Eli AMSON AG Vergleichende Zoologie Institut für BiologieHumboldt Universität zu BerlinBerlinGermany Museum für Naturkunde Berlin Leibniz Institute for Evolution and Biodiversity ScienceBerlinGermany AG Paläobiologie und Evolution Institut für BiologieHumboldt Universität zu BerlinBerlinGermany IPANEMA CNRSministère de la CultureUVSQMNHNUSR3461UniversitéParis-SaclayGif-sur-YvetteFrance UniversitéParis-Saclay ENS Paris-SaclayCNRSPPSMGif-sur-YvetteFrance Department of Zoology Swedish Museum of Natural HistoryStockholmSweden PorphyChem SAS DijonFrance ICMUB UMR CNRS 6302Universitéde Bourgogne Franche-ComtéFrance Norwegian Porphyria Centre(NAPOS) Haukeland University HospitalNorwegian Organization for Quality Improvement of Laboratory Examinations(Noklus)and Department of Global Public Health and Primary CareFaculty of MedicineUniversity of BergenNorway Institute of Earth Sciences University of LausanneLausanneSwitzerland Staatliches Museum für Naturkunde Stuttgart StuttgartGermany

Examples of photoluminescence(Pl)are being reported with increasing frequency in a wide range of organisms from diverse ***,the chemical basis of this Pl remains poorly deﬁned,and our understanding of its potential ecological function is still superﬁ*** mammals,recent analyses have identiﬁed free-base por-phyrins as the compounds responsible for the reddish ultraviolet-induced photoluminescence(UV-Pl)observed in the pelage of springhares and ***,the localization of the pigments within the hair largely remains to be ***,we use photoluminescence multispectral imaging emission and excitation spec-troscopy to detect,map,and characterize porphyrinic compounds in skin appendages in *** also document new cases of mammalian UV-Pl caused by free-base porphyrins in distantly related *** distribution of the UV-Pl is strongly suggestive of an endogenous origin of the porphyrinic *** argue that reddish UV-Pl is predominantly observed in crepuscular and nocturnal mammals because porphyrins are ***-sequently,this phenomenon may not have a speciﬁc function in intra-or interspeciﬁc communication but rather represents a byproduct of potentially widespread physiological processes.

关键词： in situ analysis mammal multispectral imaging porphyrin UV photoluminescence

The Effect of low Night and High Day Temperatures on Photosynthesis in Tomato

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2013年第12期4卷 2323-2331页

作者： Arne B.Hückstadt A.Suthaparan l.M.mortensen H.R.Gislerod Department of Plant and Environmental Sciences The Norwegian University of Life ScienceAsNorway

If low night temperatures can be combined with high day temperatures, providing optimal growth conditions for plants, a significant energy saving can be achieved in greenhouses. lowering the night temperature from 18°C to 10°C-11°C for 8 h had no negative effect on the CO2 exchange rate (CER) during the following light period in tomato. This was found both in plants grown in artificial light only or in combination with daylight. Allowing the temperature to increase from 20°C to about 40°C, in parallel with an increasing solar photon flux density (PFD) from 0 up to about 800 μmol·m-2·s-1 in the greenhouse during summer, progressively increased CER when the CO2 concentration was maintained at 900 μmol·mol-1. At 400 μmol·mol-1 CO2, maximum CER was reached at about 600 μmol·m-2·s-1 PFD combined with a temperature of 32°C, and leveled out with a further increase in PFD and temperature. Maximum CER at high CO2 concentration was around 100% higher than at low CO2 level. Under early autumn conditions, CER increased up to about 500 μmol·m-2·s-1 PFD/32°C at low CO2 and up to about 600 μmol·m-2·s-1 PFD/35°C at high CO2. An elevated CO2 level doubled the CER in this experiment as well. Measurements of chlorophyll fluorescence showed no effect of low night temperature, high day temperature or CO2 concentration on the quantum yield of photosynthesis, indicating that no treatment negatively affected the efficiency of the photosynthetic apparatus. The results showed that low night temperatures may be combined with very high day temperatures without any loss of daily photosynthesis particularly in a CO2 enriched atmosphere. If this can be combined with normal plant development and no negative effects on the yield, significant energy savings can be achieved in greenhouses.

关键词： Carbon Exchange Rate(CER) Chlorophyll Fluorescence CO2 Concentration Day Temperature Night Temperature Photon Flux Density(PFD) Solanum lycopersicon l.

High-efficiencygenomeediting in plants mediated by a Cas9 gene containing multiple introns

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant Communications 2021年第2期2卷 63-77页

作者： Ramona Grützner Patrick Martin Claudia Horn Samuel.mortensen Erin J.Cram Carolyn W.T.lee-Parsons Johannes Stuttmann Sylvestre Marillonnet Department of Cell and Metabolic Biology Leibniz Institute of Plant BiochemistryWeinberg 306120 Halle(Saale)Germany Institute for Biology Department of Plant GeneticsMartin Luther University Halle-WittenbergWeinbergweg 1006120 Halle(Saale)Germany Department of Biology Northeastern UniversityBostonMAUSA Department of Chemistry and Chemical Biology Northeastern UniversityBostonMAUSA Department of Chemical Engineering Northeastern UniversityBostonMAUSA

The recent discovery of the mode of action of the CRISPR/Cas9 systemhas provided biologists with a useful tool for generating site-specific mutations in genes of *** plants,site-targeted mutations are usually obtained by the stable transformation of a Cas9 expression construct into the plant *** efficiency of introducing mutations in genes of interest can vary considerably depending on the specific features of the constructs,including the source and nature of the promoters and terminators used for the expression of the Cas9 gene and the guide RNA,and the sequence of the Cas9 nuclease *** optimize the efficiency of the Cas9 nuclease in generating mutations in target genes in Arabidopsis thaliana,we investigated several features of its nucleotide and/or amino acid sequence,including the codon usage,the number of nuclear localization signals(NlSs),and the presence or absence of *** found that the Cas9 gene codon usage had some effect on its activity and that two NlSs worked better than ***,the highest efficiency of the constructs was achieved by the addition of 13 introns into the Cas9 coding sequence,which dramatically improved the editing efficiency of the *** of the primary transformants obtained with a Cas9 gene lacking introns displayed a knockout mutant phenotype,whereas between 70%and 100%of the primary transformants generated with the intronized Cas9 gene displayed mutant *** intronized Cas9 gene was also found to be effective in other plants such as Nicotiana benthamiana and Catharanthus roseus.

关键词： CRISPR Cas9 targeted mutagenesis gene targeting

高血压患者血钾水平与短期死亡风险:丹麦全国登记数据的回顾性分析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华高血压杂志 2016年第10期24卷 982-984页

作者： Krogager Ml Torp-Pedersen C mortensen RN Kober l Gislason G Sogaard P Aasbjerg K

欧洲高血压发病率为30%~45%,治疗率为11%~66%。多种降压药,包括利尿剂、β受体阻滞剂、血管紧张素转换酶抑制剂（angiotensin-converting enzyme inhibitor,ACEI）和血管紧张素受体拮抗剂（angiotensin receptor blocker,ARB）均会引起... 详细信息

关键词：高血压患者血钾水平登记数据死亡风险血管紧张素转换酶抑制剂血管紧张素受体拮抗剂 receptor 丹麦

去势难治性前列腺癌骨转移患者223Ra治疗间隔延长对疗效的影响

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中华核医学与分子影像杂志 2018年第7期38卷 515-516页

作者：唐军 Marie ?bro Fosbφl Peter Meidahl Petersen Gedske Daugaard Sφren Holm ANDreas Kjaer Jann mortensen 上海交通大学医学院附属苏州九龙医院核医学科 Department of Clinical Physiology Nuclear Medicine & PET and Cluster for Molecular ImagingRigshospitalet and University of Copenhagen

目的二氯化镭(223Ra)是获批用于治疗去势难治性前列腺癌（mCRPC）骨转移的发射α粒子的亲骨性放射性药物。2014年秋天,由于生产上的原因,全球223Ra缺供了2个月。该研究分析非疾病原因造成的223Ra治疗间隔延长对疗效是否有负面影响。方... 详细信息

目的二氯化镭(223Ra)是获批用于治疗去势难治性前列腺癌（mCRPC）骨转移的发射α粒子的亲骨性放射性药物。2014年秋天,由于生产上的原因,全球223Ra缺供了2个月。该研究分析非疾病原因造成的223Ra治疗间隔延长对疗效是否有负面影响。方法单中心回顾性分析了2014年3月至2015年2月间开始行223Ra治疗的mCRPC患者。记录并计算各患者的疗程数、总生存时间（OS）和放射影像学无进展生存时间（rPFS）。首次治疗前和第3次、第6次治疗后患者行骨显像、胸腹部CT、血常规检查及前列腺特异性抗原（PSA）、碱性磷酸酶测定。随访期为首次223Ra治疗后18个月。结果共50例接受223Ra治疗的mCRPC患者,其中17例（34%）因供货原因治疗间隔延长,中位延长时间为4周（范围3～9周）。治疗间隔延长患者的rPFS也显著增加[rPFS为7.1（95%CI:4.9-9.3）个月],治疗间隔未延长患者的rPFS为4.5（95%CI:2.8～6.3）个月。完成的疗程数或OS间未见明显差异。结论因非疾病相关原因造成的223Ra治疗间隔的延长对OS、rPFS或完成的疗程数均无负面影响。

关键词： 223Ra 前列腺癌骨转移放射性核素治疗

双甲脒、蝇毒磷和氟胺氰菊酯对西方蜜蜂试管幼虫慢性毒性的研究

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

双甲脒、蝇毒磷和氟胺氰菊酯对西方蜜蜂试管幼虫慢性毒性的研究

作者： Dai Pl Jack CJ mortensen AN Bustamante TA Ellis JD

在实验室条件下,测试了常见杀螨剂对西方蜜蜂Apis mellifera生存、发育速度以及幼虫重量的影响。分别给幼虫饲喂含有药物的食物,双甲脒:1.5,11,25和46 mg/l;蝇毒磷:1.8,6,8和25 mg/l或氟胺氰菊酯0.1,1,2.4和6 mg/l。对照为阳性对照、45 ...

在实验室条件下,测试了常见杀螨剂对西方蜜蜂Apis mellifera生存、发育速度以及幼虫重量的影响。分别给幼虫饲喂含有药物的食物,双甲脒:1.5,11,25和46 mg/l;蝇毒磷:1.8,6,8和25 mg/l或氟胺氰菊酯0.1,1,2.4和6 mg/l。对照为阳性对照、45 mg/l乐果;溶剂对照和阴性对照。46 mg/l双甲脒和25 mg/l蝇毒磷降低了蜜蜂的生存,氟胺氰菊酯没有影响蜜蜂生存。此外,46mg/l双甲脒处理后蜜蜂的发育率降低。我们的研究中,幼虫暴露于杀螨剂中的浓度与花粉或蜂蜜中的最大残留量相似,没有发现蜜蜂的存活率和发育速度的变化。鉴于花粉和蜂蜜和花蜜仅代表幼虫饮食的一小部分,我们认为残留的双甲脒,蝇毒磷素和氟胺氰菊酯在我们测试的水平下,不太可能影响野外未成年的工蜂生存,我们的数据并不排除蜜蜂接触这些农药后可能产生的亚致死效应。

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

百菌清和除虫脲对意蜂试管幼虫的影响

百菌清和除虫脲对意蜂试管幼虫的影响