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我国农村氟中毒地区成人氟暴露、基础代谢率、身体脂肪率与握力的关系研究

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中华地方病学杂志 2024年第1期43卷 29-34页

作者：张宣寅郭萌陈瑞琴冯子臣杨梦刘小雪余方方李志远巴月周郭育郑州大学公共卫生学院劳动卫生与环境卫生学系，郑州450001 郑州市金水区疾病预防控制中心，郑州450053 郑州大学公共卫生学院劳动卫生与环境卫生学系，郑州450001 郑州大学黄河生态保护与区域协调发展研究院，郑州450001

目的探讨我国农村氟中毒地区成人氟暴露与握力的关系以及基础代谢率(BMR)和身体脂肪率(BFP)在氟暴露与握力关联中的作用。方法2017年4-5月,采用整群抽样方法,以河南省开封市饮水型地方性氟中毒流行地区通许县的18~60岁居民作为调查对象... 详细信息

目的探讨我国农村氟中毒地区成人氟暴露与握力的关系以及基础代谢率(BMR)和身体脂肪率(BFP)在氟暴露与握力关联中的作用。方法2017年4-5月,采用整群抽样方法,以河南省开封市饮水型地方性氟中毒流行地区通许县的18~60岁居民作为调查对象,进行问卷调查、体格检查和生物样本采集。共纳入调查对象1168人,其中男性427人、女性741人。采用氟离子选择电极法、苦味酸法分别测定尿氟和尿肌酐浓度,并计算尿肌酐调整后尿氟浓度(CUF);生物电阻抗法测定BMR和BFP;Jamar测力计测量握力。采用广义线性模型分析CUF、BMR、BFP和握力之间的关系,使用中介效应模型分析BMR、BFP在CUF与握力关联中的中介效应。结果CUF每升高1.00 mg/l,女性握力降低0.28 kg(P=0.043),在男性中二者关联无统计学意义(P=0.744)。无论性别分层与否,BMR与握力均呈正向关联(均P<0.001)。BFP每升高1.00%,女性握力降低0.18 kg(P=0.043)。中介效应模型分析结果显示,BMR、BFP在CUF与女性握力关联中的中介效应比分别为65.1%(P<0.001)和8.4%(P=0.111)。结论氟暴露与女性握力改变有关,且BMR改变在氟暴露与女性握力的关联中发挥部分中介作用。

关键词：氟成人握力基础代谢率身体脂肪率

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野生和养殖锦鲤肠道微生物多样性及功能预测

山东农业大学学报（自然科学版） 2024年第2期55卷 218-227页

作者：赵园园古应慧孟金柱梁正其梅杰宋兴超巴家文铜仁学院贵州省梵净山地区生物多样性保护与利用重点实验室贵州铜仁554300

为探究不同生活环境下锦鲤肠道微生物的差异,本研究采用16S rRNA高通量测序技术对野生和养殖锦鲤肠道微生物组成进行分析。结果表明,锦鲤肠道微生物中的优势门为变形菌门(Proteobacteria,29.81%)、蓝细菌门(Cyanobacteria,25.57%)、绿... 详细信息

为探究不同生活环境下锦鲤肠道微生物的差异,本研究采用16S rRNA高通量测序技术对野生和养殖锦鲤肠道微生物组成进行分析。结果表明,锦鲤肠道微生物中的优势门为变形菌门(Proteobacteria,29.81%)、蓝细菌门(Cyanobacteria,25.57%)、绿弯菌门(Chloroflexi,18.16%);不同环境下锦鲤肠道菌群在数量和群落结构上都有明显差异,在属水平上,养殖环境下优势菌属有红细菌属(Rhodobacter)、藤黄色杆菌属(luteolibacter)、泉发菌属(Crenothrix),野生环境下优势菌属有绿丝菌属(Chloronema)、纤发鞘丝蓝细菌属(leptolyngbya)、暖绳菌属(Caldilinea);在种水平上,养殖环境下优势菌有Methylobacterium adhaesivum、琥珀黄杆菌(Flavobacterium succinicans)、胭脂甲杆菌(Methylobacterium komagatae)、湖泊玫瑰单胞菌(Roseomonas lacus);野生环境下优势菌有空腔污水球菌(Defluviicoccus vanus)、Proteocatella sphenisci、湖泊玫瑰单胞菌、Roseomonas massiliensis、索氏鲸杆菌(Cetobacterium somerae);α多样性分析显示养殖环境下锦鲤肠道微生物的多样性以及物种丰富度均比野生样本高;肠道微生物功能预测显示,l1、l2、l3水平最富集的KEGG通路分别是新陈代谢、氨基酸代谢和萜类和类固醇的生物合成,不同环境下差异显著的KEGG通路包括2-氧羧酸添加代谢、甜菜碱生物合成、安莎霉素的生物合成、双酚降解、丁酸盐代谢等。本研究从肠道微生物角度探究了锦鲤的环境适应性,为其健康养殖提供了新思路。

关键词：锦鲤环境肠道微生物功能预测

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Salt effect on molecular orientation at air/liquid methanol interface

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Chinese Chemical letters 2016年第4期27卷 535-539页

作者： Xia li Gang-Hua Deng Rong-Juan Feng Ke lin Zhen Zhang Yu ba Zhou lu Yuan Guo Beijing National laboratory for Molecular Sciences State Key Laboratory of Molecular Reaction Dynamics Institute of Chemistry Chinese Academy of Sciences Hefei National laboratory for Physical Sciences at the Microscale University of Science and Technology of China School of Physics and Optoelectronic Engineering Xidian University

The salt effects on molecular orientation at air/liquid methanol interface were investigated by the polarization-dependent sum frequency generation vibrational spectroscopy（SFG-VS）. We clarified that the average tilting angle of the methyl group to be u = 308 58 at the air/pure methanol surface assuming a d-function orientational distribution. Upon the addition of 3 mol/l Na I, the methyl group tilts further away from the surface normal with a new u = 418 38. This orientational change does not explain the enhancement of the SFG-VS intensities when adding Na I, implying the number density of the methanol molecules with a net polar ordering in the surface region also changed with the Na I concentrations. These spectroscopic findings shed new light on the salt effects on the surfaces structures of the polar organic solutions. It was also shown that the accurate determination of the bulk refractive indices and Raman depolarization ratios for different salt concentrations is crucial to quantitatively interpret the SFG-VS data.

关键词： Surface orientation Air/liquid interface Salt effect Sum frequency generation Methanol

“水城小黄姜”芽茎尖组培脱毒与快繁体系的建立

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分子植物育种 2024年第4期22卷 1170-1178页

作者：吕金丽吕树鸣张冬莲罗凯吴迪李伟贵州大学农学院贵阳550025 六盘水市农业科学研究院六盘水553000 贵州大学蔬菜研究院贵阳550025

为建立“水城小黄姜”芽茎尖组培脱毒快繁体系,本试验以“水城小黄姜”芽茎尖为外植体,研究不同消毒时间、不同诱导分化培养基、不同增殖培养基及不同生根培养基对茎尖消毒、愈伤组织诱导、不定芽增殖、生根的影响,并对组培苗进行脱毒... 详细信息

为建立“水城小黄姜”芽茎尖组培脱毒快繁体系,本试验以“水城小黄姜”芽茎尖为外植体,研究不同消毒时间、不同诱导分化培养基、不同增殖培养基及不同生根培养基对茎尖消毒、愈伤组织诱导、不定芽增殖、生根的影响,并对组培苗进行脱毒效果的检测。结果表明:“水城小黄姜”芽茎尖采用75%医用酒精消毒60 s+10%次氯酸钠(NaClO)消毒20 min处理效果最好,污染率7.7%,成活率达87.2%;不同培养基配比对茎尖诱导分化效果有差异,以MS+6-ba 3.5 mg/l+NAA 0.1 mg/l效果最佳,愈伤诱导率达75.0%;最适增殖培养基为MS+6-ba 3.0 mg/l+NAA 0.3 mg/l,增殖系数大,长势旺盛、茎粗芽壮、叶绿,组培苗生长好,且可与生根协同接续;适宜生根的培养基为MS+NAA 0.10 mg/l,生根数多,平均根长适中。用双抗体夹心酶联免疫法(ElISA)测定组培苗中烟草花叶病毒(TMV)、黄瓜花叶病毒(CMV),获得了“水城小黄姜”脱毒核心苗,且脱毒率达93.75%。因此,本研究认为通过“水城小黄姜”芽茎尖脱毒组培快繁体系的建立,可为“水城小黄姜”种苗的脱毒快繁及工厂化育苗提供参考。

关键词：小黄姜组织培养茎尖脱毒病毒检测

FAM72基因家族与肝细胞癌预后及免疫浸润的相关性分析

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岭南现代临床外科 2024年第1期24卷 14-25页

作者：林坤鹏巴明臣唐云强王嘉康林晓纯广州医科大学附属肿瘤医院肝胆外科广州510095 广州市第一人民医院体检中心广州510180

目的探讨FAM72基因家族在肝细胞癌(HCC)中的表达情况及其与预后、免疫浸润的相关性。方法应用TCGA分析FAM72基因家族在HCC中的表达情况、与不同临床特征间的差异及其预后价值;TCGA和Kaplan⁃Meier plotter分析FAM72基因家族对HCC患者总... 详细信息

目的探讨FAM72基因家族在肝细胞癌(HCC)中的表达情况及其与预后、免疫浸润的相关性。方法应用TCGA分析FAM72基因家族在HCC中的表达情况、与不同临床特征间的差异及其预后价值;TCGA和Kaplan⁃Meier plotter分析FAM72基因家族对HCC患者总生存期的影响;STRING构建FAM72基因家族蛋白相互作用网络;ssGSEA分析FAM72基因家族与免疫浸润细胞间的相关性;GEPIA分析FAM72基因家族与免疫细胞分子标志物及免疫检查点的相关性。结果HCC中FAM72⁃D的表达量显著高于正常肝组织(Pl1、CTlA⁃4)有正相关关系。结论FAM72基因家族在HCC组织中高表达,并与免疫浸润、不良预后相关,可作为HCC预后的预测指标。

关键词：肝细胞癌 FAM72基因家族预后免疫浸润生物信息学分析

Bilharzia Appendicitis: Incidence in a Commune in Mali, about 3CAS

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Surgical Science 2024年第5期15卷 321-329页

作者： Souleymane Bingue Dembélé Yely Dianessy Moussa Samake Abdou Guiré Siaka Konaté Ousmane Sy Boubacar Yoro Sidibé Amadou Maïga Thiorno Nadio Boubacar Karembe Amaguiré Saye Sirama Diarra Dorcas Sodjiné Yede Issiaka Diarra Ibrahima Maiga Mahamane Dicko Moussa Konaté Aliou Bina Diarra bakary Tientigui Dembélé General Surgery Department of the District Hospital of Commune IV of bamako Bamako Mali Kolondié ba Reference Health Centre Sikasso Mali Epidemiology and Clinical Research Bamako Mali General Surgery Department of Gabriel Touré University Hospital Bamako Mali Ophthalmology Department of the District Hospital of Commune IV of bamako Bamako Mali Gynecology and Obstetrics Department of the District Hospital of Commune IV of bamako Bamako Mali Commune I of the District of bamako Reference Health Centre Bamako Mali Imaging Department of the District Hospital of Commune IV of bamako Bamako Mali Commune VI of the District of bamako Reference Health Centre Bamako Mali Anotomy-Pathology Department of the G Point University Hospital Bamako Mali

Appendicitis bilharzia is a very rare condition and we report 3 cases of this pathology that sense clinical and biological similarities with bacterial appendicitis. The etiological diagnosis was exclusively histopathological, this allowed to highlight eggs of Schistosome in the appendicular wall in the three patients. The surgical treatment was supplemented by a specific medical treatment based on praziquantel. The surgical suites were simple for 2 patients, complicated by superficial parietal suppuration in a patient.

关键词： Bilharzial Appendicitis Endemic Histopathology Specific Antiparasitic Treatment

Effect of harvest time on the chemical composition and antioxidant capacity of Gannan navel orange(Citrus sinensis l. Osbeck 'Newhall')juice

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Journal of Integrative Agriculture 2022年第1期21卷 261-272页

作者： ZHANG Jun ZHANG Jing-yi SHAN You-xia GUO Can HE lian ZHANG lin-yan lING Wei lIANG Yan ZHONG ba-lian National Engineering Research Center of Navel Orange Gannan Normal UniversityGanzhou 341000P.R.China South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650P.R.China

The present study investigates the chemical composition and antioxidant capacity of juice from the Gannan navel orange, which is harvested at one-to two-week intervals during the ripening period. The total soluble solid(TSS), total polyphenol content(TPC), total flavonoid content(TFC), sucrose and hesperidin contents gradually increase with the ripening of the fruit, followed by slight declines at the late maturity stage. Contrary to these observations, the contents of titratable acid(TA), vitamin C(Vc), and limonin trend downward throughout the ripening period. However, the contents of fructose, glucose, and narirutin fluctuate throughout the harvest time. Three in vitro antioxidant assays consistently indicate that the harvest time exerts no significant influence(P>0.01) on the antioxidant capacity. Furthermore, principal component analysis(PCA) and Pearson’s correlation test are performed to provide an overview of the complete *** study provides valuable information for evaluating the fruit quality and determining when to harvest the fruit in order to meet the preferences of consumers. Meanwhile, our observations suggest that the fruits subjected to juice processing should be harvested at the late maturity stage to alleviate the "delayed bitterness" problem without compromising the antioxidant capacity and the flavonoid content in the juice.

关键词： navel orange antioxidant harvest time chemical composition

Influence of Constructed Wetland and Soil Filter Systems in the Dynamics of Phytoplankton Functional Groups of Two Subtropical Fish Farm Wastewaters

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Journal of Water Resource and Protection 2014年第1期6卷 8-15页

作者： Rodrigo N.Millan lúcia H.Sipaúba-Tavares Fernanda Travaini-lima Aquaculture Center University of Sao Paulo StateJaboticabalBrazil Federal University of Sao Carlos Sao CarlosBrazil

Environmental pressure, land utilization, and economic feasibility have resulted in the development of alternatives to treatment fish farm wastewater. We examined the influence of two water treatment systems—a constructed wetland (CW) and a soil filter system (SF)—on the constitution of the phytoplankton community analyzed through the functional grouping of species and nutrients removal of aquaculture farm. The CW provided high removal efficiency: 82.9% for ammonia, 87.0% for nitrate, 96.9% for nitrite, 85.5% for total phosphorus, 88.5% for SRP and 71.6% for BOD. Removal efficiency of SF was lower than CW, removing 82.1% of ammonia, 7.2% of total phosphorus, 45.9% of SRP and 39.4% of nitrite, but was satisfactory. The functional group F, made up of Chlorophyceae Dictyosphaerium pulchellum and Kirchneriella lunaris, was the most representative in both systems, followed by functional group P and constituted by Zygnemaphyceae Melosira sp. The number of functional groups decreased in the outlet of the two treatment systems, where S1, H1 and W1, characteristic of eutrophic environment, were retained. Data show that CW and the SF system are potentially applicable to the fish farm wastewater treatment and ensure an improvement in water quality.

关键词： Wastewater Treatment Microalgae Removal Efficiency Functional Groups

2019款迈腾B8l轿车电子节气门组件故障检测与初始化

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汽车实用技术 2024年第16期49卷 172-176页

作者：张家辉王峰鲁亚云薛喜红巴寅亮新疆职业大学智慧交通学院新疆乌鲁木齐830013

在汽车故障检测的实际操作过程中,会发现有时仅仅通过一种检测方法并不能准确确定故障发生的原因和位置,无法排除故障,给维修带来一定的困扰。为能够更好地检测汽车传感器故障,文章针对发动机电子节气门组件的实际检测方法进行了详细介... 详细信息

在汽车故障检测的实际操作过程中,会发现有时仅仅通过一种检测方法并不能准确确定故障发生的原因和位置,无法排除故障,给维修带来一定的困扰。为能够更好地检测汽车传感器故障,文章针对发动机电子节气门组件的实际检测方法进行了详细介绍,并对2019款迈腾B8l轿车的节气门位置传感器故障进行检测和分析,排除故障并进行节气门初始化,为维修行业及高职院校教学提供汽车维修思路。

关键词：节气门位置传感器节气门初始化故障检测