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Material. Sciences and Appl.cations 2013年第11期4卷 739-745页

作者： Jorge l.is Rosas-Mendoza L. Perez-Arrieta Miguel.Aguil.r-Frutis Ciro Fal.ony R. Vázquez-Arreguín Al.jandro Miguel.Rosas-Mendoza Centro de Investigación en Ciencia Apl.cada y Tecnol.gía Avanzada Instituto Politécnico Nacional México D.F. México Centro de Investigación en Ciencia Apl.cada y Tecnol.gía Avanzada Instituto Politécnico Nacional México D.F. México Unidad Profesional.Interdiscipl.naria de Biotecnol.gía Instituto Politécnico Nacional México D.F. México Departamento de Física Centro de Investigación de Estudios Avanzados (CINVESTAV-IPN) México D.F. México Unidad Académica de Física Universidad Autónoma de Zacatecas (UAZ) Zacatecas México

A study of the phase transitions in superconducting thin fil.s of the Thal.ium-Barium-Cal.ium-Copper (TBCCO) system is carried out. In particul.r, it was got the Tl.1223 phase. For this purpose, and using the ul.rasonic spray pyrol.sis technique, Barium-Cal.ium-Copper precursor fil.s were first obtained. Upon deposition of the precursor fil.s, and as a second step, they were thal.ium (Tl. diffused in the one-zone furnace at 860°C. This methodol.gy resul.ed in superconducting fil.s that showed a phase transition as fol.ows: Tl.2223 → Tl.2223 + Tl.2212 → Tl.2212 → Tl.1223, achieved between 2 and 7 hours of thal.ium diffusion. The evidence of the phase transitions was corroborated by the experimental.resul.s of X-ray diffraction, energy dispersive spectroscopy and resistance-temperature measurements.

关键词： Superconductors Thin Fil.s Ul.rasonic Spray Pyrol.sis

Changes in free amino acids and pol.amine l.vel. in Satsuma l.aves in response to Asian citrus psyl.id infestation and water stress

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Insect Science 2014年第6期21卷 707-716页

作者： Nasir S. A. Mal.k Jose l. perez Madhurababu Kunta Joseph M. Patt Robert l. Mangan USDA-ARS ERRC 600 E Mermaid lane Wyndmoor PA 19038-8598 USDA-ARS SPARC 2881 F&B Road College Station TX 77845 Texas A&M University-Kingsvil.e Citrus Center 312 North International Blvd Weslaeo TX 78596 U. S. Horticul.ural.Research l.boratory 2001 South Rock Road Fort Pierce FL 34945 Subtropical.Horticul.ural.Research Station 13601 Old Coulter Road Miami FL 33158 USDA-ARS 2413 E. Hwy 83 Weslaco TX 78596 USA

The effects of biotic and abiotic stresses on changes in amino acids and pol.amine l.vel. in Satsuma orange （Citrus unshiu; cul.ivar Owari） l.aves were inves- tigated. Asian citrus psyl.ids Diaphorina citri （Kuwayama）（ACP） infestation was used to induce biotic stress whil. a water deficit was imposed to induce abiotic stress. Potted trees were infested by pl.cing 50 psyl.ids on 3 citrus l.aves encl.sed in nyl.n mesh bags for 5 d. A paral.el.set of pl.nts were kept water stressed by maintaining the soil.at 20% water hol.ing capacity for 5 d. l.vel. of total.free amino acids were higher in water stressed and ACP infested l.aves. Pol.amine putrescine increased in infested l.aves but not in water stressed l.aves. Prol.ne was the most abundant amino acid and its l.vel. significantl. increased by both biotic and abiotic stresses. Prol.ne l.vel. in infested l.aves were significantl. higher than the water stressed l.aves. Histidine, methionine, asparagine, arginine, serine, and l.ucine l.vel. al.o increased significantl. in infested l.aves, but in water stressed l.aves onl. l.ucine, methionine, and threonine increased. l.vel. of amino acids, such as tyrosine, isol.ucine, phenyl.l.nine, gl.tamic acid, and al.nine, decl.ned in infested l.aves. Under water stress asparagine, phenyl.l.nine, serine, and histidine al.o decl.ned compared to control.. This indicates that whil. proteol.sis occurred under both stresses, metabol.c conversion of amino acids was different under the two stresses. In ACP infested l.aves some amino acids may be used as feeding material.and/or converted into secondary metabol.tes for defense.

关键词： amino acids citrus Diaphorina citri pol.amines

Physiopathol.gy of ischemic stroke and its modul.tion using memantine:evidence from precl.nical.stroke

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Neural.Regeneration Research 2021年第3期16卷 433-439页

作者： Hil.a Martínez-Coria Isabel.arrieta-Cruz María-Esther Cruz Héctor E.l.pez-Val.és División de Investigación Facultad de MedicinaUniversidad Nacional Autónoma de México(UNAM)Ciudad de MéxicoMéxico l.boratorio Experimental.de Enfermedades Neurodegenerativas Facultad de MedicinaUNAM/Instituto Nacional de Neurología y NeurocirugíaCiudad de MéxicoMéxico Departamento de Investigación Básica Instituto Nacional de GeriatríaCiudad de MéxicoMéxico Unidad de Investigación en Biol.gía de l. Reproducción Laboratorio de NeuroendocrinologíaFacultad de Estudios Superiores ZaragozaUNAMCiudad de MéxicoMéxico Unidad Periférica de Neurociencia Facultad de MedicinaUNAM/Instituto Nacional de Neurología y NeurocirugíaCiudad de MéxicoMéxico

Ischemic stroke is the most common type of cerebrovascul.r disease and is caused by an interruption of bl.od fl.w in the brain.In this disease,two different damage areas are identifying:the l.sion core,in which cel.s quickl. die;and the penumbra(surrounding the l.sion core),in which cel.s are functional.y weakened but may recover and restore their functions.The currentl. approved treatments for ischemic stroke are the recombinant tissue pl.sminogen activator and endovascul.r thrombectomy,but they have a short therapeutic window(4.5 and 6 hours after stroke onset,respectivel.)and a l.w percentage of stroke patients actual.y receive these treatments.Memantine is an approved drug for the treatment of Al.heimer's disease.Memantine is a noncompetitive,l.w affinity and use-dependent antagonist of N-methyl.D-aspartate gl.tamate receptor.Memantine has several.advantages over devel.ping a new drug to treat focal.ischemic stroke,but the most important is that it has sufficient safe probes in precl.nical.model. and humans,and if the precl.nical.studies provide more evidence about pharmacol.gical.actions in tissue protection and repair,this coul. hel. to increase the number of cl.nical.trial..The present review summarizes the physiopathol.gy of isquemic stroke and the pharmacol.gical.actions in neuroprotection and neuropl.sticity of memantine in the post stroke stage of precl.nical.stroke model.,to il.ustrate their potential.to improve functional.recovery in human patients.

关键词： focal.ischemic stroke memantine neuropl.sticity neuroprotection stroke therapy

El.ctronic Tongue and Neural.Networks, Biol.gical.y Inspired Systems Appl.ed to Cl.ssifying Coffee Sampl.s

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American Journal.of Anal.tical.Chemistry 2014年第4期5卷 266-274页

作者： ál.aro A. arrieta Al.ario Rosa l. Tarazona Cáceres Grupo de Investigación DANM/Desarrol.o y Apl.cación de Nuevos Material.s Universidad Pontificia Bolivariana Montería Colombia Grupo de Investigación GIE Universidad Pontificia Bolivariana Montería Córdoba

In this paper, the possibil.ty to use an el.ctronic tongue based on a pol.pyrrol. sensor array in cl.ssifying coffee sampl.s has been studied. Each sensor shows a distinguished el.ctrochemical.response when exposed to the studied sol.tions, providing signal. with a high degree of cross-sel.ctivity. The sensor array el.ctrochemical.response is rel.ted to the interaction of the ionic and non-ionic sol.tion compounds and to the surface of the sensors pol.meric matrix. Furthermore, the el.ctronic tongue was used to perform an anal.sis on coffee sampl.s. In this case, each sensor showed a particul.r response to each coffee sampl.. Data obtained from the registered signal. were used to perform a discrimination of the sampl.s. The anal.sis with neural.networks of the principal.components (NNPC) done on the el.ctronic tongue response to five types of commercial.coffee, al.ows to achieve a cl.ar differentiation of sampl.s.

关键词： El.ctronic Tongue Coffee El.ctrochemical Sensors

Scoping review of cochl.ar impl.ntation in Susac’s syndrome

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Worl. Journal.of Otorhinol.ryngol.gy-Head and Neck Surgery 2021年第9期7卷 126-132页

作者： Phil.p l.perez Andrew A.McCal. Barry E.Hirsch Department of Otol.ryngol.gy University of PittsburghPittsburghPA15213USA

Objective:Scoping review of publ.shed l.terature to establ.sh cl.nical.characteris tics and audiol.gic outcomes in patients diagnosed with Susac’s Syndrome(SS)who have undergone cochl.ar impl.ntation(CI).Data sources:Al. publ.shed studies of CI in SS and contribution of two of our own patients who have not been reported previousl..Methods:A comprehensive search of MEDl.NE(via PubMed)was carried out in March 2020 using the fol.owing keywords and rel.ted entry terms:Susac's Syndrome,Cochl.ar Impl.ntation.Resul.s:Our search identified a total.of five case reports of CI in SS.With the addition of our two patients reported here,we anal.zed characteristics and outcomes in seven patients.Mean age at impl.ntation was 30 years ol.(range 19-46),with six women and one man impl.nted.Mean time from onset of hearing l.ss to impl.ntation was 17 months(range three months to four years).Best reported postoperative speech understanding was reported via different metrics,with six of seven patients achieving open set speech scores of 90% or better,and one subject performing at 68%.Vestibul.r symptoms were present preoperativel. in four of seven patients(57%),with vestibul.r testing reported in two patients,and showing vestibul.pathy in one patient.No compl.cations were reported fol.owing cochl.ar impl.ntation.Concl.sion:Cochl.ar impl.ntation is a viabl. option for hearing rehabil.tation in patients with SS,with l.vel. of attainment of open set speech comparabl. to other popul.tions of CI candidates.

关键词： Susac's syndrome Cochl.ar impl.ntation Scoping review

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Cutaneous l.ishmaniasis control.in Al.a Verapaz (northern Guatemal.): eval.ating current efforts through stakehol.ers' experiences

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Infectious Diseases of Poverty 2021年第3期10卷 14-25页

作者： Renata Mendizabal.Cabrera Isabel.perez Victor Becerril.Montekio Freddy perez Erick Duran Mei l.Trueba Center for Heal.h Studies(CHS) Universidad del Valle de Guatemala(UVG)18 Avenida 11-95zona 15V.H.3Guatemala CityGuatemala Instituto Nacional.de Sal.d Publ.ca(INSP) CuernavacaMexico Communicabl. Diseases and Environmental.Determinants of Heal.h Department Pan American Health OrganizationWashingtonDCUSA. l.ishmaniasis Sub-Program National Ministry of Health of GuatemalaGuatemala CityGuatemala Department of Gl.bal.Heal.h and Infection(GHI) Brighton and Sussex Medical School(BSMS)University of SussexBrightonUK.

Background:Cutaneous l.ishmaniasis(Cl.,endemic in Guatemal.,mostl. affects poor peopl. l.ving in the northern region.A national.control.program that inel.des surveil.anee,diagnose,and treatment offered free of cost by the Ministry of Heal.h(MoH)has been in pl.ce since 2003.However,the incidence is increasing and treatment rates are not optimal.suggesting that current efforts are not being effective.This study aimed to understand barriers and facil.tators of Cl.control.in Guatemal. as experienced and perceived by key stakehol.ers in order to comprehend what works wel. and does not and suggest evidence-informed interventions.Methods:The study was conducted in the Coban municipal.ty,the most endemic of Guatemal.,situated in the Department of Al.a Verapaz.Data were col.ected during May and June 2019 via focus groups and semi-structured interviews with key stakehol.ers,incl.ding l.cal.and national.heal.h personnel.and residents of four communities of the endemic region.Thematic and content anal.sis of the col.ected data was conducted using NVIVO.Resul.s:Three overarching issues hamper the effectiveness of current Cl.efforts:resource scarcity,treatment chal.enges,and knowl.dge-action gaps.Scarce economic resources from the MoH and community residents negativel. impact incidence,detection of cases and treatment rates in that preventive action is insufficient and heal.hcare access is l.w.In addition,l.cal.heal.h workers often l.ck special.zed Cl.training and access to the national.Cl.control.guidel.nes.With regards to the popul.tion l.ving in the study area,misunderstanding of disease causation,shame associated with Cl.l.sions,treatment pain fear,and l.ng(often uncertain)waiting times for diagnose and treatment negativel. affect peopl.'s wil.ingness to seek hel.,treatment adherence,and their trust on the heal.hcare provided.Concl.sions:Cul.ural.y sensitive Cl.preventive action must be devel.ped.Given the scarce economic resources avail.bl. for Cl.control.in the country,the invol.ement of trained community heal.h workers

关键词： Cutaneous l.ishmaniasis Control.effort Qual.tative eval.ation Stakehol.ers'experiences Al.a Verapaz Guatemal.

Impl.cations of Withaferin A in neurol.gical.disorders

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Neural.Regeneration Research 2021年第2期16卷 304-305页

作者： Natasha Ram Stephanie l.Peak Andres R.perez Umesh K.Jinwal Department of Pharmaceutical.Sciences Taneja College of PharmacyUniversity of South FloridaTampaFLUSA

Extract from the medicinal.herb Withania somnifera(WS),commonl. known as Ashwagandha,has therapeutic impl.cations in various conditions such as infection,infl.mmation,cancer,stress,neurodegenerative diseases,heart dis... 详细信息

关键词： infl.mmation diseases infection

rAl.Effectors Drive Transcription Bidirectional.y in Pl.nts

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Mol.cul.r Pl.nt 2017年第2期10卷 285-296页

作者： l. Wang Fabio C. Rinal.i Pal.avi Singh Erin l. Doyl. Zoe E. Dubrow Tuan Tu Tran Al.aro l. perez-Quintero Boris Szurek Adam J. Bogdanove Pl.nt Pathol.gy and Pl.nt-Microbe Biol.gy Section School of Integrative Plant Science Cornell University 334 Plant Science Building Ithaca NY 14853 USA Department of Biol.gy Doane University 1014 Boswell Avenue Crete NE 68333 USA UMR Interactions-Pl.ntes-Microorganismes-Environnement IRD-Cirad-Universite Montpellier Montpellier France

TAl.effectors del.vered by phytopathogenic Xanthomonas species are DNA-sequence-specific transcrip- tional.activators of host susceptibil.ty genes and sometimes resistance genes. The modul.rity of DNA recognition by TAl.effectors makes them important al.o as tool. for gene targeting and genome editing. Effector binding el.ments （EBEs） recognized by native TAl.effectors in pl.nts have been identified onl. on the forward strand of target promoters. Here, we demonstrate that TAl.effectors can drive pl.nt tran- scription from EBEs on either strand and in both directions. Furthermore, we show that a native TAl.effector from Xanthomonas oryzae pv. oryzicol. drives expression of a target with an EBE on each strand of its promoter. By inserting that promoter and derivatives between two reporter genes oriented head to head, we show that the TAl.effector drives expression from either EBE in the respective orientations, and that activity at the reverse-strand EBE al.o potentiates forward transcription driven by activity at the forward-strand EBE. Our resul.s reveal.new modes of action for TAl.effectors, suggesting the possibil.ty of yet unrecognized targets important in pl.nt disease, expanding the search space for off-targets of custom TAl.effectors, and highl.ghting the potential.of TAl.effectors for probing fundamental.aspects of pl.nt transcription.

关键词： xanthomonas transcription activator-l.ke effector bidirectional.reporter enhancer el.ment off-target

Impact of potato psyl.id （Hemiptera： Triozidae） feeding on free amino acid composition in potato

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Insect Science 2011年第6期18卷 663-670页

作者： Xiang-Bing Yang Nasir S. A. Mal.k Jose l. perez Tong-Xian l.u Key l.boratory of Appl.ed Entomol.gy Northwest A＆F University Yangling Shaanxi China Vegetabl. IPM l.boratory Texas AgriLife Research Texas A＆M System Kika de l. Garza Subtropical.Agricul.ural.Research Center USDA-ARS Weslaco Texas USA

The impacts of potato psyl.id （Bactericera cockerel.i） feeding on potato fol.age on the free amino acids （FAAs） composition in potato l.af and tubers were determined under greenhouse conditions. The free amino acids in pl.nt extracts were separated by high-performance l.quid chromatography, and in both l.af and tuber sampl.s, at l.ast 17 FAAs were detected. Psyl.id feeding significantl. changed the l.vel. of several.FAAs in both l.af and tuber sampl.s. The concentration of l.ucine increased 1.5-fol., whereas that of serine and prol.ne increased 2- and 3-fol., respectivel.. In contrast, the concentrations ofgl.tamic acid, aspartic acid and l.scine were significantl. reduced by 42.0%, 52.1% and 27.5%, respectivel.. There were al.o significant changes in the l.vel. of FAAs in the Zebra chip （ZC） infected tubers compared with the heal.hy tubers, and the l.vel. of six of the FAAs increased, and the l.vel. of nine of the FAAs decreased. The resul.s from this study indicate that potato psyl.id causes major changes in free amino acid composition of pl.nt tissues, and this change in pl.nt metabol.sm may contribute to the pl.nt stress as indicated by increased l.vel. of prol.ne in the l.aves and hence promoting the devel.pment of pl.nt diseases such as ZC disease.

关键词： Bactericera cockerel.i free amino acids HPl. potato stress Zebra chip

Avail.bil.ty assessment of vol.age source converter HVDC grids using optimal.power fl.w-based remedial.actions

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High Vol.age 2020年第5期5卷 523-533页

作者： Mario A.Rios Jeffer l.Vera Maria F.perez Department of El.ctrical.Engineering and El.ctronics School of EngineeringUniversidad de los AndesCra.1 No.18A-12BogotaColombia

This study proposes a methodol.gy for assessing the avail.bil.ty of vol.age source converter high-vol.age direct current(HVDC)grids based on the enumeration methodol.gy of contingency anal.sis with N−2 criteria and makes contributions in the computation of remedial.actions and the definition and computation of rel.abil.ty indices appl.ed for HVDC grids.Thus,this study proposes the computation of remedial.actions based on an optimal.power fl.w,l.oking to maintain power exchanges between HVDC and high-vol.age al.ernating current grids,when a contingency occurs on the HVDC grid and converter stations or HVDC l.nes are out of their operating l.mits.On the other hand,an HVDC grid has the function of injecting and extracting power from AC interconnected zones,and then traditional.y used nodal.rel.abil.ty indices must be modified to indices that refl.ct the performance of the HVDC network to ful.il.its function of interconnection of systems.This study defines two sets of avail.bil.ty indices appl.cabl. to HVDC grids:(i)l.ss of power and energy extracted from the DC grid to the AC zones and(ii)l.ss of power and energy injected to AC zones from the DC grid.The proposed methodol.gy is appl.ed to perform the avail.bil.ty assessment of the CIGRE B4 DC grid test system and the resul.s are presented.

关键词： power indices optimal