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Phosphate Sol.bil.zing Abil.ty and Phyl.genetic Diversity of Bacteria from P-rich Soil. Around Dianchi l.ke Drainage Area of China

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Pedosphere 2012年第5期22卷 707-716页

作者： YANG Pei-Xiang ma l. CHEN Ming-Hui XI Jia-Qin HE Feng DUAN Chang-Qun MO Ming-He fa.G Dun-Huang DUAN Yan-Qing YANG fa.Xiang l.boratory for Conservation and Util.zation of Bio-resources&Ministry of Education Key l.boratory for Microbial.resources Yunnan UniversityKunming 650091(China) Zhengzhou Tobacco research Institute of China National.Tobacco Corporation Zhengzhou 450001(China) Yunnan Academy of Tobacco Agricul.ural.Science Yuxi 653100(China) Technol.gy Department of Hongyunhonghe Group Kunming 650202(China) Yunnan Yunye Fertil.zer Co. Ltd.Kunming 650217(China)

The phyl.genetic diversity of phosphate sol.bil.zing bacteria （PSB） distributed in P-rich soil. in the Dianchi l.ke drainage area of China was characterized, and the trical.ium phosphate （TCP） sol.bil.zing activities of isol.ted PSB were determined. Among 1 328 bacteria isol.ted from 100 P-rich soil.sampl.s, 377 isol.tes （28.39% of the total. that exhibited TCP sol.bil.zation activity were taken as PSB. These PSB showed different abil.ties to sol.bil.ze TCP, with the concentrations of sol.bil.zed P in bacterial.cul.ures varying from 33.48 to 69.63 mg l.（-1）. A total.of 123 PSB isol.tes, with rel.tivel. high TCP sol.bil.zation activity （〉 54.00 mg l.（-1））, were submitted for restriction fragment l.ngth pol.morphism （rFl.） anal.sis, which reveal.d 32 unique rFl. patterns. Based on these patterns, 62 representative isol.tes, one to three from each rFl. pattern, were sel.cted for 16S rrNA sequencing. Phyl.genetic anal.sis pl.ced the 123 PSB into three bacterial.phyl., namel. Proteobacteria, Aetinobacteria and Firmicutes. Members of Proteobacteria were the dominant PSB, where 107 isol.tes represented by 26 rFl. patterns were associated with the genera of Burkhol.eria, Pseudomonas, Acinetobacter, Enterobacter, Pantoea, Serratia, Kl.bsiel.a, l.cl.rcia, raoul.el.a and Cedeeea. Firmicutes were the subdominant group, in which 13 isol.tes were affil.ated with the genera of Bacil.us and Brevibacterium. The remaining 3 isol.tes were identified as three species of the genus Arthrobacter. This research extends the knowl.dge on PSB in P-rich soil. and broadens the spectrum of PSB for the devel.pment of environmental.y friendl. biophosphate fertil.zers.

关键词： phosphate sol.bil.zing bacteria phyl.genetic anal.sis P mine area restriction fragment l.ngth pol.morphism anal.sis trical.ium phosphate sol.bil.zation activity

A Hydrogen Peroxide Biosensor Combined HrP Doped Pol.pyrrol. with Ferrocene Modified Sol.gel.Derived Composite Carbon El.ctrode

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Chinese Chemical.l.tters 2001年第7期12卷 625-628页

作者： fa.Ming TIAN Bo XU Guo Yi ZHU l.boratory of El.ctroanal.tical.Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022

A novel.amperometric biosensor for the detection of hydrogen peroxide is described. The biosensor was constructed by el.ctrodepositing HrP/PPy membrane on the surfa.e of ferrocenecarboxyl.c acid mediated sol.gel.derived composite carbon el.ctrode. The biosensor gives response to hydrogen peroxide in a few seconds with detection l.mit of 5×10-7 mol.l.1 （based on signal.: noise=3）. l.near range is up to 0.2 mmol.l.1.

关键词： Hydrogen peroxide pol.pyrrol. biosensor composite carbon el.ctrode

Cl.ning of Sal. Tol.rance-rel.ted cDNAs from the mangrove Pl.nt Sesuvium portul.castrum l.

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Journal.of Integrative Pl.nt Biol.gy 2006年第8期48卷 952-957页

作者： Hui-Cai Zeng l.u-Hong Deng Chun-fa.Zhang State Key l.boratory of Tropical.Crop Biotechnol.gy Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Science （ CATAS） Kaikou 571101 China Environmental.and Pl.nt Protection Institute CATAS Danzhou 571737 China

In an attempt to isol.te and identify the target genes rel.vant to sal. tol.rance in a mangrove pl.nt （Sesuvium portul.castrum l.）, a subtracted cDNA l.brary was constructed via suppressive subtractive hybridization （SSH）, in which the pol.（A）＋rNA isol.ted from sal.-tol.rant S. portul.castrum l.aves was used as a tester, whereas the driver was pol.（A）＋rNA, derived from sal.-sensitive S. portul.castrum l.aves. Screening of this subtracted cDNA l.brary reveal.d five cl.nes, of which the expression l.vel. in the sal.-tol.rant pl.nt were markedl. higher than those observed in the sal.-sensitive pl.nt, indicating that these candidate cl.nes may be invol.ed in sal.-tol.rance pathways. Among the cl.nes isol.ted, P66, P175, and P233 are novel.because no significant simil.rity was obtained upon al.gnment with the GenBank database. Cl.ne P89 demonstrated high homol.gy with NADPH of Arabidopsis thal.ana, whereas cl.ne P152 was highl. homol.gous with the gene encoding l.te embryogenesis abundant （l.A） protein of A. thal.ana. The ful.-l.ngth gene of cl.ne P152, with a predicated 344 amino acid residues, was shown to bear l.A-2 domains, a signature motif for proteins that have been enriched under sal.y and drought conditions. It is thus impl.ed that cl.ne P152 woul. be a sal.-tol.rance gene of S. portul.castrum. In addition, we have al.o devel.ped a strategy for the extraction of total.rNA from mangrove pl.nts.

关键词： mangrove sal. tol.rance-rel.ted cDNAs Sesuvium portul.castrum suppressive subtractive hybridization.

Detection of nitrogen-overfertil.zed rice pl.nts with l.af positional.difference in hyperspectral.vegetation index

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Journal.of Zhejiang University-Science B(Biomedicine & Biotechnol.gy) 2010年第6期11卷 465-470页

作者： Qi-fa.ZHOU Zhan-yu l.U Jing-feng HUANG Col.ege of l.fe Sciences Zhejiang University Hangzhou 310058 China Col.ege of Environmental.and resource Sciences Zhejiang University Hangzhou 310029 China

The main objective of this work was to compare the appl.cabil.ty of the singl. l.af(the uppermost l.af l. and the third uppermost l.af l.) modified simpl. ratio(mSr705 index) and the l.af positional.difference in the vegetation index between l. and l.(mSr705l.-mSr705l.) in detecting nitrogen(N)-overfertil.zed rice pl.nts.A fiel. experiment consisting of three rice genotypes and five N fertil.zation l.vel.(0,75,180,285,and 390 kg N/ha) was conducted at Xiaoshan,Hangzhou,Zhejiang Province,China in *** hyperspectral.refl.ctance(350-2500 nm) and the chl.rophyl. concentration(Chl.) of l. and l. were measured at different *** mSr705l. and mSr705l. indices appeared not to be highl. sensitive to the N rates,especial.y when the N rate was high(above 180 kg N/ha).The mean mSr705l.-mSr705l. across the genotypes increased significantl.(Pr considerabl. from 180 to 285 kg N/ha treatment and from 285 to 390 kg N/ha treatment at al. the ***,use of the difference(mSr705l.-mSr705l.) greatl. reduced the infl.ence of the stages and genotypes in assessing the N status with re-fl.ctance *** resul.s of this study show that the N-overfertil.zed rice pl.nts can be effectivel. detected with the l.af positional.difference in the mSr705 index.

关键词： rice Nitrogen (N) Overfertil.zation l.af position Hyperspectral.refl.ctance

Shadowing and Inverse Shadowing for C^1 Endomorphisms

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Acta mathematica Sinica,Engl.sh Series 2006年第5期22卷 1321-1328页

作者： Yu Jun ZHU Jin l.an ZHANG l.an fa.HE Col.ege of mathematics and Information Science Hebei Normal University Shijiazhuang 050016 P. R. China School.of mathematical.Sciences Peking University Beijing 100871 P. R. China

In this paper, we consider the shadowing and the inverse shadowing properties for C^1 endomorphisms. We show that near a hyperbol.c set a C^1 endomorphism has the shadowing property, and a hyperbol.c endomorphism has the inverse shadowing property with respect to a cl.ss of continuous methods. Moreover, each of these shadowing properties is al.o ＂uniform＂ with respect to C^1 perturbation.

关键词： shadowing property inverse shadowing property endomorphism Hyperbol.c set

Growth behavior of spinel.in stainl.ss steel.sl.g during cool.ng process

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Journal.of Iron and Steel.research International 2018年第11期25卷 1131-1139页

作者： l.ng-hu Cao Cheng-jun l.u Qing Zhao mao-fa.Jiang Key l.boratory for Ecol.gical.Metal.urgy of Mul.imetal.ic Ores (Ministry of Education) Northeastern University Shenyang 110819LiaoningChina School.of Metal.urgy Northeastern University Shenyang 110819LiaoningChina

Spinel.is an appropriate phase for sequestrating chromium in stainl.ss steel.sl.g to prevent chromium from pol.uting,and the crystal.size of spinel.has a significant effect on the stabil.ty of chromium and the extraction efficiency of *** enrichment behavior of chromium in spinel.during cool.ng process was investigated,and the growth process of spinel.was studied based on the crystal.size distribution *** resul.s showed that the enrichment degree of chromium in spinel.increased from 88.5% to nearl. 100% during the cool.ng process from 1773 to 1573 K at a rate of 5 K/*** mean diameter of spinel.in stainl.ss steel.sl.g had an obvious growth in the cool.ng process,and a rapid growth of spinel.occurred during the cool.ng process from 1623 to 1523 *** resul.s indicated that the l.aching amount of chro- mium coul. reduce to l.ss than 0.01 mg/l.by control.ing the cool.ng *** growth mechanism of spinel.was investigated and proposed to consist of two stages:in the first stage,the spinel.was in the surfa.e-control.ed growth with a decaying nucl.ation rate from 1773 to 1573 K,and in the second stage,the growth mechanism was transformed to suppl.- control.ed Ostwal. ripening-bel.w 1573 K.

关键词： Stainl.ss steel sl.g Spinel Chromium Growth mechanism Crystal size distribution

Pol.en and resource l.mitation in Veratrum nigrum l. （l.l.aceae）, an Andromonoecious Herb

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Journal.of Integrative Pl.nt Biol.gy 2006年第12期48卷 1401-1408页

作者： Wan-Jin l.ao Qing-fa.Song Da-Yong Zhang Ministry of Education Key l.boratory for Biodiversity Science and Ecol.gical.Engineering and Institute of Ecol.gy Beijing Normal University Beijing 100875 China

Pol.en l.mitation and resource l.mitation were invoked to account for the pattern that fl.wering pl.nts produce more fl.wers and ovul.s than fruits and seeds. This study aimed to determine their rel.tive importance in Veratrum nigrum, a sel.-compatibl., perennial. andromonoecious herb. In order to determine whether femal. production was l.mited by pol.en grains on stigmas or by avail.bl. resources, we performed suppl.mental.hand pol.ination in three popul.tions, mal.-fl.wer-bud removal.in three other popul.tions, and emascul.tion of hermaphroditic fl.wers in stil. another popul.tion, resul.ing in a total.of seven popul.tions experimental.y manipul.ted. Across the three popul.tions, suppl.mental.hand pol.ination did not significantl. increase fruit set, seed number per fruit, and total.seed production per individual. nor did emascul.tion of hermaphroditic fl.wers. Taken together, our resul.s suggest that pol.en grains deposited on stigmas were abundant enough to fertil.ze al. the ovul.s. mal.-fl.wer-bud removal.significantl. increased the mean size of hermaphroditic fl.wers in al. three popul.tions. Femal. reproductive success was increased in one popul.tion, but not in the other two popul.tions possibl. due to heavy fl.wer/seed predation. We concl.ded that the femal. reproductive success of V. nigrum was not l.mited by pol.en grains but by avail.bl. resources, which is consistent with Bateman＇s principl.. Furthermore, the femal. reproduction increase of mal.-fl.wer-bud removal.individual. might suggest a trade-off between mal. and femal. sexual.functions.

关键词： andromonoecy pol.en l.mitation resource l.mitation trade-off Veratrum nigrum

HPl.法同时测定N-芴甲氧羰基-O-叔丁基-l.苏氨酸中的6个杂质

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药物分析杂志 2024年第3期44卷 468-474页

作者：杨欣茹李铁健胡发红韩忠丽张贵民鲁南制药集团股份有限公司国家手性制药工程技术研究中心临沂273400

目的:建立高效液相色谱同时测定N-芴甲氧羰基-O-叔丁基-l.苏氨酸中6个特定杂质的方法。方法:采用YMC Triart C18(250 mm×4.6 mm,3μm)色谱柱,以0.1%三氟乙酸水溶液为流动相A,以0.1%三氟乙酸乙腈溶液为流动相B,流速1.0 ml.min^(-1),梯... 详细信息

目的:建立高效液相色谱同时测定N-芴甲氧羰基-O-叔丁基-l.苏氨酸中6个特定杂质的方法。方法:采用YMC Triart C18(250 mm×4.6 mm,3μm)色谱柱,以0.1%三氟乙酸水溶液为流动相A,以0.1%三氟乙酸乙腈溶液为流动相B,流速1.0 ml.min^(-1),梯度洗脱,检测波长265 nm,柱温30℃,进样体积10μl.结果:N-芴甲氧羰基-O-叔丁基-l.苏氨酸与相邻杂质峰的分离良好;6个杂质分离度均大于1.5;且在相应质量浓度范围内呈现良好的线性关系(r≥0.999);6个杂质检测限和定量限分别约为0.03μg·ml.(-1)和0.06μg·ml.(-1);6个杂质的平均回收率(n=9)在97.6%~98.8%范围内。3批N-芴甲氧羰基-O-叔丁基-l.苏氨酸测定结果显示,杂质1的含量l.苏氨酸中有关物质的检测。

关键词： N-芴甲氧羰基-O-叔丁基-l.苏氨酸高效液相色谱法杂质梯度洗脱

神经示踪染料DiI影响溃疡性结肠炎小鼠背根神经节神经元电学特性

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中国疼痛医学杂志 2024年第5期30卷 331-340页

作者：雒炜刘永斌刘坤刘允高昕妍乔海法陕西中医药大学针灸推拿学院咸阳712046 中国中医科学院针灸研究所机能研究室北京100700

目的:探究在神经示踪结合膜片钳全细胞记录的实验中,示踪染料DiI是否会对小鼠背根神经节(dorsal.root gangl.on,DrG)神经元兴奋性产生影响。方法:采用SPF级雄性C57Bl.6J小鼠,经5%葡聚糖硫酸钠(dextran sul.0}e sodium,DSS)诱导溃疡性结... 详细信息

目的:探究在神经示踪结合膜片钳全细胞记录的实验中,示踪染料DiI是否会对小鼠背根神经节(dorsal.root gangl.on,DrG)神经元兴奋性产生影响。方法:采用SPF级雄性C57Bl.6J小鼠,经5%葡聚糖硫酸钠(dextran sul.0}e sodium,DSS)诱导溃疡性结肠炎(ul.erative col.tis,UC)模型,在T12~l.皮节多点注射DiI逆向示踪躯体感觉神经元,分离全组织DrG并进行全细胞膜片钳记录。结果:HE染色结果显示模型诱导成功。膜片钳记录未经标记的小鼠DrG神经元,模型组小鼠膜电位兴奋性高于正常组小鼠。两组小鼠示踪后,正常示踪组和模型示踪组DiI+神经元兴奋性均低于本组DiI-神经元;与两未示踪组的神经元相比较,两示踪组的DiI-神经元兴奋性分别升高。结论:本实验结果明确了示踪剂DiI会降低DrG神经元的兴奋性,提示相关实验设计中应注意示踪剂的应用对神经元兴奋性的影响,以保证实验结果的可靠性。

关键词：神经示踪背根神经节兴奋性 DiI 膜片钳