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Profile Analysis of the Proteome of the Egg of the High Royal Jelly Producing Bees (Apis mellifera l.)

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Agricultural Sciences in china 2007年第9期6卷 1138-1148页

作者： ZHANG Ean lI Jian-ke wu li-ming Department of Bioengineering Zhengzhou University Zhengzhou 450001 P.R.China Institute of Apicultural Research Chinese Academy of Agricultural Sciences Beijing 100093 P.R.China

The protein composition of the egg development in the high royal jelly producing bees （Apis mellifera l.） was investigated. This pioneer study was to separate and quantify the proteins in the egg of the high royal jelly producing worker bees （Apis mellifera l.） by using two-dimensional gel electrophoresis along with their three-day development. The results showed that 160, 195, and 176 proteins, with a wide range of molecular weight （17-80 KDa） and relatively narrow scope of pI （4. 00-8.40） could be detected on day 1, day 2, and day 3, respectively, during the developmental process of the egg. Meanwhile 44 protein spots were constantly detected along with the egg development. Among them 36% were in the uptrend along with the egg development, 14% were in the downtrend, and 39% were of the largest expressed volume on day 2. In addition, the specific proteins were expressed on day 1, day 2, and day 3 （89, 77, and 80, respectively）. Besides the coexistent and specific proteins, 24 proteins were expressed on day 1 and day 2, but silenced on day 3, 49 proteins were expressed on day 2 and day 3, but silenced on day 1, only 3 proteins were expressed on day 1 and day 3, but silenced on day 2. The result indicates that egg development is a sequential and complex gene controlled process, where the eggs of day 2 express the most active proteins. The coexistent proteins suggest that it is conservative and indispensable for this event. These specific proteins suggest that the different developmental stage needs specific proteins to regulate it.

关键词： honeybees high royal jelly producing worker bee's egg two-dimensional gel electrophoresis proteome

MPPa-PDT induced apoptosis and autophagy through JNK and p38 MAPK signaling pathways in A549 cells

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BIOcEll 2024年第11期48卷 1603-1612页

作者： PINGHUA TU SHANSHAN WANG KElAN DENG XINJUN lI ZHANlING wu Department of Respiratory and critical care Medicine Xiaogan Hospital Afﬁliated to Wuhan University of Science and TechnologyDepartment of Respiratory and Critical Care MedicineXiaogan432000China

Objectives:The antitumor effects of pyropheophorbide-αmethyl ester-mediated photodynamic therapy(MPPa-PDT)were observed in several *** objective of this investigation was to examine the antineoplastic efﬁcacy of MPPa-PDT acting on lung carcinoma A549 cells and further elaborate ***:The viability of A549 cells was examined with cell counting kit-8 after MPPa-PDT *** 33342 staining,monodansylcadaverine(MDc)staining,and transmission electron microscopy were employed to observe apoptotic bodies and autophagic *** cytometry with Annexin V/propidium iodide(PI)labeling objectively assessed cell *** expression of associated proteins,including caspase-3,Beclin-1,lc-3II,and mitogen-activated protein kinase(MAPK)families concluding c-jun NH2-terminal kinase(JNK),p38 MAPK,and extracellular signal-regulated kinase 1/2(ERK)were identiﬁed by Western ***:Prolonged exposure to MPPa-PDT gradually decreased lung cancer A549 cell *** and autophagy activity were higher in the MPPa-PDT cohort in comparison to the control ***,autophagy inhibition enhanced cell-killing efﬁcacy ***,the JNK and p38 MAPK pathways were implicated in MPPa-PDT-triggered apoptosis and ***:This study demonstrated that JNK and p38 MAPK were engaged in MPPa-PDT-mediated apoptosis and autophagy in lung carcinoma A549 cells.

关键词： Photodynamic therapy lung carcinoma Apoptosis Autophagy

Enhancing the thermostability of D-allulose 3-epimerase from clostridium cellulolyticum H10 via directed evolution

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Systems Microbiology and Biomanufacturing 2022年第4期2卷 685-694页

作者： Shuhan liu Yifan Wang Demin Kong Jing wu Zhanzhi liu State Key laboratory of Food Science and Technology Jiangnan University1800 Lihu AvenueWuxi 214122Jiangsu ProvinceChina School of Biotechnology and Key laboratory of Industrial Biotechnology Ministry of Education Jiangnan University1800 Lihu AvenueWuxi 214122Jiangsu ProvinceChina International Joint laboratory On Food Safety Jiangnan University1800 Lihu AvenueWuxi 214122Jiangsu ProvinceChina

d-allulose,the epimer at c-3 position of d-fructose,is a low-calorie functional rare sugar,which is regarded as one of the most potential *** present,the main production method of d-allulose is epimerization of d-fructose by d-allulose 3-epimerase(DAE).However,industrial applications of DAE are still limited by its poor ***,directed evolution was applied to improve the thermostability of dAE from clostridium cellulolyticum H10(ccDAE).Two optimal mutants D281G and c289R,exhibiting 13.80-fold and 13.88-fold t_(1/2 )values as that of wild type at 65℃,respectively,were *** further enhance the thermostability,the triple mutant A107P/D281G/c289R was constructed after combina-tion of mutants D281G,c289R,and previously identified thermostability-enhanced mutant *** T_(m) and optimal temperature of triple mutant were increased by 14.39℃and 5℃,respectively,compared to the wild type,meanwhile,the half-life of triple mutant was 58.85-fold as that of wild type at 65℃.Furthermore,the conversion rate of triple mutant was increased from 24.76%of wild type to 27.53%using 300 g/l d-fructose as substrate at 70℃.The effectiveness of directed evolution was verified and the triple mutant with enhanced thermostability had great application value in the large-scale production of d-allulose.

关键词： D-allulose 3-epimerase Directed evolution Ultra-high throughput screening Thermostability

Genetic relationship between purebred and crossbred sow longevity

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Journal of Animal Science and Biotechnology 2017年第1期8卷 24-29页

作者： c.E.Abell R.l.Fernando T.V.Serenius M.F.Rothschild K.A.Gray K.J.Stalder Department of Animal Science Iowa State University Ames IA 50011-3150 USA Figen ltd. PO Box 31960101 Seinajoki Finland Smithfield Premium Genetics 316 W. Charity Rd Rose Hill NC 28458 USA

Background： The overall breeding objective for a nucleus swine selection program is to improve crossbred commercial performance. Most genetic improvement programs are based on an assumed high degree of positive relationship between purebred performance in a nucleus herd and their relatives＇ crossbred performance in a commercial herd. The objective of this study was to examine the relationship between purebred and crossbred sow longevity performance. Sow longevity was defined as a binary trait with a success occurring if a sow remained in the herd for a certain number of parities and including the cumulative number born alive as a measure of reproductive success. Heritabilities, genetic correlations, and phenotypic correlations were estimated using ***： Results indicated little to no genetic correlations between crossbred and purebred reproductive *** indicates that selection for longevity or lifetime performance at the nucleus level may not result in improved longevity and lifetime performance at the crossbred level. Early parity performance was highly correlated with lifetime performance indicating that an indicator trait at an early parity could be used to predict lifetime performance. This would allow a sow to have her own record for the selection trait before she has been removed from the ***： Results from this study aid in quantifying the relationship between purebred and crossbred performance and provide information for genetic companies to consider when developing a selection program where the objective is to improve crossbred sow performance. Utilizing crossbred records in a selection program would be the best way to improve crossbred sow productivity.

关键词： Binary traits Sow longevity Threshold model

Antibody and complement levels in patients with hypersplenism associated with cirrhotic portal hypertension and therapeutic principles

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World Journal of clinical cases 2022年第36期10卷 13208-13215页

作者： Kun Zhang Min Zeng Ye-Juan li Hong-Fei wu Jin-cai wu Zhen-Sheng Zhang Jin-Fang Zheng Yun-Fu lv Department of Geriatric center Hainan General HospitalHaikou 570311Hainan ProvinceChina Reproductive Medicine center Maternal and Child Health Care Hospital of Hainan ProvinceHaikou 570311Hainan ProvinceChina Department of General Surgery Hainan General HospitalHaikou 570311Hainan ProvinceChina

BAcKGROUND Hypersplenism associated with cirrhotic portal hypertension is a common condition often resulting from hepatitis B-related ***,the levels of immunoglobulin(Ig)and complement in patients with hypersplenism associated with cirrhotic portal hypertension remain *** study was undertaken to determine the levels of Ig and complement in these patients,the relationship between these levels and child-Pugh class and their clinical *** To investigate the antibody(Ig)and complement levels in patients with hypersplenism associated with cirrhotic portal hypertension and their clinical *** clinical data of 119 patients with hypersplenism associated with cirrhotic portal hypertension were statistically analyzed and compared with those of 128 control *** IgA and IgG levels in patients with hypersplenism were significantly higher than controls(Pcant difference in IgM between the two groups(P=0.109).c3 and c4 levels in patients with hypersplenism were significantly lower than controls(Pliver function decreased,IgA and IgG levels increased(Pc3 and c4 levels decreased(PcONclUSION Patients with hypersplenism associated with cirrhotic portal hypertension have significantly higher antibody(IgA and IgG)levels and significantly lower complement(c3 and c4)levels,which are both related to liver ***,the administration of anti-hepatitis virus agents and protection of liver function should be strengthened.

关键词： Hypersplenism associated with cirrhotic portal hypertension complement Treatment Hepatitis B-immunoglobulin

carbon dioxide exchange processes over the grassland ecosystems in semiarid areas of china

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Science china Earth Sciences 2012年第4期55卷 644-655页

作者： DU Qun lIU HuiZhi WANG lei HUANG JianPing ZHANG wu christian BERNHOFER State Key laboratory of Atmospheric Boundary layer Physics and Atmospheric chemistry Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Graduate University of chinese Academy of Sciences Beijing 100049China college of Atmospheric Sciences Lanzhou UniversityLanzhou 730000China Institute of Hydrology and Meteorology Dresden University of TechnologyDresden 01737Germany

Based on the carbon fluxes measured over the grassland ecosystems in Inner Mongolia （UG79 site）, loess Plateau （SAcOl site）, and Tongyu, Jilin Province （TY site） in the semiarid areas from 2007 to 2008 with the eddy covariance method, we have investigated the carbon exchange processes over semiarid grassland ecosystem and its main affecting environmental variables. The precipitations at UG79 and TY sites in 2007 were below the historical average, especially for TY site, which was 50% be- low the historical average annual precipitation. The precipitation in SAcOl site was close to average in 2007 but below average in 2008. The variation of monthly diurnal average NEE showed that the diurnal mean NEE decreased in the order of TY site, UG79 site, and SAcOl site. However, a longer net carbon uptake period was observed at SAcOl site. The diurnal course of NEE at UG79 site was similar between 2007 and 2008. The diurnal average NEE remained large during July and August in growing sea- son （May to September） at UG79 site, with maximum values approaching 0.08 mg c m^-2 s^-1 in August of 2008. The diurnal av- erage NEE of 2007 was larger than 2008 at SAcOl site, with maximum values of 0.07 mg c m^-2 sq in September of 2007. A shorter carbon uptake period was recorded in 2007 at TY site, lasting from July to August. A larger diurnal average NEE oc- curred in 2008 at TY site, with maximum values of 0.12 mg c m^-2 s^-1. The ecosystem respirations of three sites were controlled by both soil temperature and soil volumetric water content （at a depth of 5 cm below the land surface）. Both UG79 site and SAcOl site acted as a carbon sink during the growing periods of 2007 and 2008. Annual NEE in the growing seasons of 2007 and 2008 ranged from -68 to -50 g c m^-2 at UG79 site and from -109 to -55 g c m^-2at SAcOl site. Alternation between car- bon source and carbon sink was found at TY site, with respective values of annual NEE in the growing seasons of 0.32 g c m^-2 and -73 g c m^-2 i

关键词： semiarid grassland ecosystem carbon flux eddy covariance method

Six novel species of Fusarium from natural ecosystems in Australia

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Fungal Diversity 2016年第2期77卷 349-366页

作者： M.H.laurence J.l.Walsh l.A.Shuttleworth D.M.Robinson R.M.Johansen T.Petrovic T.T.H.Vu l.W.Burgess B.A.Summerell E.c.Y.liew The Royal Botanic Gardens and Domain Trust Mrs Macquaries RdSydneyNSW 2000Australia Faculty of Agriculture and Environment The University of SydneyEveleighNSW 2015Australia

Six new species of Fusarium associated with soil and plant hosts from ecosystems of minimal anthropogenic disturbance in Australia are *** coicis from coix gasteenii,*** from Xanthorrhoea glauca,*** from soil and Mangifera indica,*** from soil,*** from Sorghum interjectum and *** from soil,Triodia microstachya,Sorghum interjectum and Sorghum *** and phylogenetic analysis of EF-1α,RPB1 and RPB2 sequence data were used to delineate species *** new species were phylogenetically distributed in the Fusarium sambucinum,***,and *** species complexes,and two novel species *** six new species have particular phylogeographic significance as not only do they provide further insight into the geographic patterns of Fusarium evolution but also challenge current phylogeographic hypotheses.

关键词： Species diversity Endophyte Phylogenetics Mycoflora Phylogeography

large and anisotropic linear magnetoresistance in bulk stoichiometric cd_3As_2 crystals

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Science china(Physics,Mechanics & Astronomy) 2015年第1期58卷 133-138页

作者： wu DeSheng WANG Xia ZHANG Xu YANG chongli ZHENG Ping lI PeiGang SHI YouGuo Department of Physics Center for Optoelectronics Materials and Devices Zhejiang Sci-Tech University Beijing National laboratory for condensed Matter Physics Institute of Physics Chinese Academy of Sciences

cd3As2 was recently identified as a novel three-dimensional （3D） topological semimetal hosting the long-pursuing 3D Dirac Fermion. crystals of cd3As2 grown preferentially along the [100] and [112] directions were obtained through the modified chemical vapor transfer growth method, thus allowing the examination of transport anisotropy. The resistivity and magnetore- sistance （MR） are basically linear with respect to magnetic field （H） in the measured temperature range of 2-300 K irrespective of the directions. The linear resistivity and MR are significantly anisotropic not only along [100] and [112] directions but also with respect to tilt angle between the growth directions and H, thus providing transport signatures of the 3D Dirac Fermion as well as the possible linear and anisotropic change of Weyl Fermi surface in H. Very large MR along the [100] direction is observed, even approaching 3100% at 2 K and 14 kOe （10e = 79.5775 A m^-l）. The results would be helpful in renewing interest in studying emergent phenomena arising from bulk 3D Dirac Fermion as well as in paving the way for cd3As2 to be used in magnetoelectronic sensors.

关键词： topological semimetal linear magnetoresistance transport anisotropy

Determination of potential management zones from soil electrical conductivity,yield and crop data

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第1期9卷 68-76页

作者： Yan lI Zhou SHI ci-fang wu Hong-yi lI Feng lI college of Southeast land Management Zhejiang University Hangzhou 310029 China Institute of Agricultural Remote Sensing and Information Technology Application Zhejiang University Hangzhou 310029 China Department of Environmental Engineering Zhejiang University Hangzhou 310029 China

One approach to apply precision agriculture to optimize crop production and environmental quality is identifying management zones. In this paper,the variables of soil electrical conductivity (Ec) data,cotton yield data and normalized differ-ence vegetation index (NDVI) data in an about 15 ha field in a coastal saline land were selected as data resources,and their spatial variabilities were firstly analyzed and spatial distribution maps constructed with geostatistics technique. Then fuzzy c-means clustering algorithm was used to define management zones,fuzzy performance index (FPI) and normalized classification entropy (NcE) were used to determine the optimal cluster numbers. Finally one-way variance analysis was performed on 224 georefer-enced soil and yield sampling points to assess how well the defined management zones reflected the soil properties and produc-tivity level. The results reveal that the optimal number of management zones for the present study area was 3 and the defined management zones provided a better description of soil properties and yield variation. Statistical analyses indicate significant differences between the chemical properties of soil samples and crop yield in each management zone,and management zone 3 presented the highest nutrient level and potential crop productivity,whereas management zone 1 the lowest. Based on these findings,we conclude that fuzzy c-means clustering approach can be used to delineate management zones by using the given three variables in the coastal saline soils,and the defined management zones form an objective basis for targeting soil samples for nutrient analysis and development of site-specific application strategies.

关键词： Management zones Fuzzy clustering Spatial variability Saline land Precision agriculture