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PKD3 promotes metastasis and growth of oral squamous cell carcinoma through positive feedback regulation with PD-l1 and activation of ERK-STAT1/3-EMT signalling

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International Journal of Oral Science 2021年第1期13卷 79-88页

作者： Bomiao Cui Jiao Chen Min luo Yiying liu Hongli Chen Die lü liwei Wang Yingzhu Kang Yun Feng libin Huang Ping Zhang State Key laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology Sichuan UniversityChengduChina Department of Gastroenterology West China HospitalSichuan UniversityChengduChina

Oral squamous cell carcinoma(OSCC)has a high incidence of *** immunotherapy targeting PD-l1 or PD-1 has been revolutionary;however,only a few patients with OSCC respond to this ***,it is essential to gain insights into the molecular mechanisms underlying the growth and metastasis of *** this study,we analysed the expression levels of protein kinase D3(PKD3)and PD-l1 and their correlation with the expression of mesenchymal and epithelial *** found that the expression of PKD3 and PD-l1 in OSCC cells and tissues was significantly increased,which correlated positively with that of mesenchymal markers but negatively with that of epithelial *** PKD3 significantly inhibited the growth,metastasis and invasion of OSCC cells,while its overexpression promoted these *** further analyses revealed that there was positive feedback regulation between PKD3 and PD-l1,which could drive EMT of OSCC cells via the ERK/STAT1/3 pathway,thereby promoting tumour growth and ***,silencing PKD3 significantly inhibited the expression of PD-l1,and lymph node metastasis of OSCC was investigated with a mouse footpad xenograft ***,our findings provide a theoretical basis for targeting PKD3 as an alternative method to block EMT for regulating PD-l1 expression and inhibiting OSCC growth and metastasis.

关键词： metastasis inhibited squamous

Chemical components in yellow mealworm(Tenebrio molitor l.)reared with tea powder

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Journal of Future Foods 2022年第3期2卷 266-269页

作者： Yanxia Wang Hongya li Yu lu Wenyi He Chen Wang litang lü Institute of Entomology Guizhou UniversityGuiyang 550025China College of Tea Science Guizhou UniversityGuiyang 550025China Guizhou Minzu University Guiyang 550025China

The yellow mealworms(Tenebrio molitor l.)were reared by different ratios of tea powder and wheat bran powder mixtures,and weighted every day during the whole 10 days *** content of tea polyphenols,total catechins,catechin(C),epicatechins(EC),epigallocatechins(EGC),epicatechin gallates(ECG),epigallocatechin gallates(EGCG),and nutrition components such as protein and fatty acid in the yellow mealworms were determined after 10 days *** yellow mealworms can put on weight and grow well when the proportion of tea powder belows 80%,while 100%tea powder rearing has no signifi cantly reduced the weight of yellow *** content of tea polyphenols,total catechins,C,EC,EGC,ECG,and EGCG all enhance signifi cantly along with the increase ratio of the tea *** protein content increase more signifi cantly at 50%and 80%ratios treatments than the other *** powder added rearing reduces the content of saturated fatty acid,and has a complex effect on the content of unsaturated fatty *** analysis of the components determined show that 50%ratio is the best for yellow mealworm rearing.

关键词： Yellow mealworm Tea Protein Fatty acid

Callus formation in relation to changes of the membrane Ca^(2+) in the graft unions of Ginkgo biloba l.

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Chinese Science Bulletin 1998年第20期43卷 1732-1736页

作者： YANG Xiong and lI Zhengli (lee C. l.) College of life Sciences, Peking University, Beijing 100871, China College of life Sciences Peking University Beijing China

The relationship between the callus formation and intracellular Ca 2+ at the early stage of development of graft unions in Ginkgo biloba l. was investigated. On the second day after grafting, part of the undamaged living cells at the graft interface of the stock and scion in the regions of the cortex and pith began to dedifferentiate or divide. 8 d after grafting, a great number of callus cells were produced from the cortex and phloem, while callus bridges linking the stock with scion formed between the callus cells of the graft partners from the cortex. A membrane Ca 2+ probe, chlortetracycline (CTC), was used to locate the intracellular Ca 2+ distribution and it was found that the cortical and pith parenchyma cells were the first to show remarkably increased CTC Ca 2+ fluorescence, suggesting that the cortical and other parenchyma cells play a leading role at the early stage of development of graft unions by earlier dedifferentiation and more rapid production of callus cells.

关键词： callus formation membrane Ca 2+ graft union Ginkgo biloba.

Assessment of molecular markers and marker-assisted selection for drought tolerance in barley(Hordeum vulgare l.)

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Journal of Integrative Agriculture 2024年第1期23卷 20-38页

作者： Akmaral Baidyussen Gulmira Khassanova Maral Utebayev Satyvaldy Jatayev Rystay Kushanova Sholpan Khalbayeva Aigul Amangeldiyeva Raushan Yerzhebayeva KulpashBulatova Carly Schramm Peter Anderson Colin l.D.Jenkins Kathleen lSoole Yuri Shavrukov Faculty of Agronomy S.Seifullin Kazakh AgroTechnical Research UniversityAstana 010000Kazakhstan A.I.Barayev Research and Production Centre of Grain Farming Shortandy 021601Kazakhstan Kazakh Research Institute of Agriculture and Plant Growing AlmalybakAlmaty District 040909Kazakhstan College of Science and Engineering Biological SciencesFlinders UniversityAdelaideSA 5042Australia

This review updates the present status of the field of molecular markers and marker-assisted selection(MAS),using the example of drought tolerance in *** accuracy of selected quantitative trait loci(QTls),candidate genes and suggested markers was assessed in the barley genome *** common strategies are described for molecular marker development,candidate gene identification and verification,and their possible applications in MAS to improve the grain yield and yield components in barley under drought *** strategies are based on the following five principles:(1)Molecular markers are designated as genomic‘tags’,and their‘prediction’is strongly dependent on their distance from a candidate gene on genetic or physical maps;(2)plants react differently under favourable and stressful conditions or depending on their stage of development;(3)each candidate gene must be verified by confirming its expression in the relevant conditions,e.g.,drought;(4)the molecular marker identified must be validated for MAS for tolerance to drought stress and improved grain yield;and(5)the small number of molecular markers realized for MAS in breeding,from among the many studies targeting candidate genes,can be explained by the complex nature of drought stress,and multiple stress-responsive genes in each barley genotype that are expressed differentially depending on many other factors.

关键词： barley candidate genes drought tolerance gene verification via expression grain yield marker-assisted selection(MAS) molecular markers quantitative trait loci(QTls) strategy for MAS

Ameliorative effects of potassium on drought-induced decreases in fiber length of cotton(Gossypium hirsutum l.) are associated with osmolyte dynamics during fiber development

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The Crop Journal 2019年第5期7卷 619-634页

作者： Wenqing Zhao Haoran Dong Rizwan Zahoor Zhiguo Zhou John l.Snider Yinglong Chen Kadambot H.M.Siddique Youhua Wang Key laboratory of Crop Ecophysiology and Management of Ministry of Agriculture Nanjing Agricultural UniversityNanjing 210095JiangsuChina Jiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP) Nanjing Agricultural UniversityNanjing 210095JiangsuChina The UWA Institute of Agriculture The University of Western AustraliaPerthWA 6001Australia The Department of Crop and Soil Sciences University of GeorgiaTiftonGA 31794USA State Key laboratory of Soil Erosion and Dryland Farming on the loess Plateau Northwest A&F UniversityYangling 712100ShannxiChina University of the Agriculture Faisalabad Sub-campus DepalpurOkaraPakistan

Fiber length of cotton(Gossypium hirsutum l.)decreases under drought stress,potassium(K)could diminish the decreased caused by drought,but the mechanism associated with this alleviation effect is not *** evaluated the effect of K on fiber elongation using two cotton cultivars,Simian 3 and Siza 3,grown in well-watered and drought-stressed *** fertilizer(K2O)was applied 0,150,or 300 kg ha?1 in each growing *** stress reduced the final fiber length due to a decline in the maximum rate of rapid elongation(Vmax,mmday?1).The application of K alleviated the droughtinduced fiber length reduction by increasing *** 10 and 15 days post-anthesis(DPA),drought significantly reduced osmotic potential(OP)and increased K+and malate contents at all K rates,relative to well-watered conditions,which was associated with increased activities of phosphoenolpyruvate carboxylase(PEPC),V-ATPase,PPase,and PM H+-ATPase in cotton ***,the relative contribution of K+and malate to OP declined under drought in comparison with well-watered *** with control without K,K application decreased OP and increased the accumulation of osmolytes(K+,malate and soluble sugar)as well as the activities of related enzymes in fiber irrespective of water ***,K application increased osmotic adjustment during drought,and improved the contribution of K+and malate to OP,especially under drought *** study showed that drought decreased fiber length by reducing Vmax,and K application ameliorates the decline in fiber elongation due to drought by enhancing osmolytes accumulation and their contribution to OP in fiber cells.

关键词： Cotton(Gossypium hirsutum) Fiber length Drought stress Potassium application Osmolyte

Effects of two medicinal plants Psidium guajava l. (Myrtaceae) and Diospyros mespiliformis l. (Ebenaceae) leaf extracts on rat skeletal muscle cells in primary culture

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第1期7卷 56-63页

作者： BElEMTOUGRI R.G. CONSTANTIN B. COGNARD C. RAYMOND G. SAWADOGO l. laboratory of Physiological Animal University of Ouagadougou BP 7021 Burkina Faso Institute of Physiology & Biology Cell University of Poitiers France

Crude decoction, aqueous and ethanolic extracts of two medicinal plants （Psidiura guajava and Diospyros raespiliformis）, widely used in the central plateau of Burkina Faso to treat many diseases were evaluated for their antagonistic effects on caffeine induced calcium release from sarcoplasmic reticulum of rat skeletal muscle cells. These different extracts showed a decrease of caffeine induced calcium release in a dose dependent manner. Comparison of the results showed that Psidiura guajava leaf extracts are more active than extracts of Diospyros mespiliformis and that crude decoctions show better inhibitory activity. The observed results could explaine their use as antihypertensive and antidiarrhoeal agents in traditional medicine, by inhibiting intracellular calcium release.

关键词： Psidium guajava Diospyros mespiliformis Myrtaceae Ebenaceae Medicinal plants Intracellular calcium Sarcoplasmic reticulum Caffeine

Cannabinoids function in defense against chewing herbivores in Cannabis sativa l.

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Horticulture Research 2023年第11期10卷 131-140页

作者： George M.Stack Stephen I.Snyder Jacob A.Toth Michael A.Quade Jamie l.Crawford John K.McKay John Nicholas Jackowetz Ping Wang Glenn Philippe Julie l.Hansen Virginia M.Moore Jocelyn K.C.Rose lawrence B.Smart Horticulture Section School of Integrative Plant ScienceCornell UniversityCornell AgriTechGenevaNY 14456United States Plant Biology Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853United States Plant Breeding Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853United States Department of Agricultural Biology Colorado State UniversityFort CollinsCO 80523United States Cirona labs GenevaNY 14456United States Department of Entomology Cornell UniversityCornell AgriTechGenevaNY 14456United States

In the decades since the first cannabinoids were identified by scientists,research has focused almost exclusively on the function and capacity of cannabinoids asmedicines and intoxicants for humans and other *** little is knownabout the adaptive value of cannabinoid production,though several hypotheses have been proposed including protection from ultraviolet radiation,pathogens,and *** test the prediction that genotypeswith greater concentrations of cannabinoidswill have reduced herbivory,a segregating F2 population of Cannabis sativa was leveraged to conduct lab-and field-based bioassays investigating the function of cannabinoids in mediating interactions with chewing *** the field,foliar cannabinoid concentration was inversely correlated with chewing herbivore *** detached leaves,Trichoplusia ni larvae consumed less leaf area and grew less when feeding on leaves with greater concentrations of *** electron and light microscopy were used to characterize variation in glandular trichome ***-free genotypes had trichomes that appeared *** isolate cannabinoids from confounding factors,artificial insect diet was amended with cannabinoids in a range of physiologically relevant *** grew less and had lower rates of survival as cannabinoid concentration *** results support the hypothesis that cannabinoids function in defense against chewing herbivores.

关键词： function founding ultraviolet

Michael David Kaulana Ing, Why Confucius Wept： A Review of The Dysfunction of Ritual in Early Confucianism （reviewed by Douglas l. Berger）

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Frontiers of Philosophy in China 2015年第2期10卷 311-314页

作者： Douglas l. Berger Southern Illinois University

Michael David Kaulana Ing, Why Confucius Wept： A Review of The Dysfunction of Ritual in Early Confucianism. New York, NJ： Oxford University Press, 2012, xii ＋ 304 pp., ISBN： *** the centrality of ritual and its relation to ethical conduct in Confucian thought, it is rather remarkable that, apart from Song dynasty-inspired fascination with the Zhongyong （中庸） and Daxue （大学） chapters, very little modern philosophical attention has been paid to the entire corpus of liji （礼记）, Record of Rights. In addition to this lacuna, it is only very recently that scholars of Religious Studies who focus on ritual have turned their attention to early Confucian conceptions of rites with an eye to incorporating these conceptions into alternative theoretical frameworks. While Michael Ing＇s truly outstanding and powerfully argued Dysfunction of Ritual--grounded both in classical Chinese and modern English-language commentary---does extrapolate a ＂tragic theory of ritual＂ which broadens discussions in current Religious Studies debates, this review will focus on the degree to which this ＂tragic theory＂ can deepen our estimation of early Confucian thought.

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Optogenetic inhibition of medial prefrontal cortex projections to the nucleus accumbens core and methyl supplementation via l-Methionine attenuates cocaine-primed reinstatement

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Integrative Zoology 2018年第6期13卷 795-803页

作者： Katherine N.WRIGHT Amanda M.DOSSAT Caroline E.STRONG lindsay l.SAIlER Samantha M.PAVlOCK Mohamed KABBAJ Department of Biomedical Sciences and Program in Neuroscience Florida State UniversityTallahasseeFloridaUSA National Institute on Drug Abuse BaltimoreMarylandUSA Department of Biomedical Sciences College of MedicineFlorida State UniversityTallahasseeFloridaUSA

DNA methylation has been identified as a powerful and activity-dependent regulator of changes in the brain that may underlie neuroadaptations in response to various types of stimuli,including exposure to drugs of ***,the medial prefrontal cortex(mPFC)projections to the nucleus accumbens(NAc)are critically important for reinstated cocaine-seeking in a rodent model of cocaine *** circuitry undergoes several epigenetic modifications following cocaine exposure,including changes in DNA methylation that are associated with drug-seeking *** have previously shown that methyl supplementation via l-Methionine(MET)administration attenuates cocaine-seeking behavior and reverses expression and methylation patterns of the immediate early gene c-fos,suggesting that MET may act by altering the excitability of this circuitry during cocaine *** the current study,male rats were microinjected with an adeno-associated virus overexpressing halorhodopsin in the mPFC,optical fibers were surgically implanted into the NAc,and the rats were given injections of MET *** underwent acquisition of cocaine self-administration(0.75 mg/kg/infusion,2-h sessions)followed by extinction training in the absence of drug-paired *** reinstatement tests were conducted:cue-induced reinstatement without optogenetic manipulations and cocaine-primed reinstatement with optogenetic inhibition of mPFC-to-NAc *** were no group differences before the cocaine-primed reinstatement session,and all groups showed robust cue-induced *** rats treated with MET and rats that received mPFC-to-NAc inhibition showed an abolishment of cocaine-primed reinstatement,suggesting that systemic methyl supplementation may act through this critical circuity.

关键词： cocaine-primed reinstatement corticolimbic DNA methylation methionine optogenetics