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Native and exotic seed dispersal by the stone marten(Martes foina):implications for the regeneration of a relict climactic forest in central Portugal

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Integrative Zoology 2019年第3期14卷 280-292页

作者： Lúcia pereira Ana VASQUES Paula MAIA Maria João RAMOS pereira Carlos FONSECA Milena MATOS CESAM-Centre for Environmental and Marine Studies-Wildlife Research Unit Department of BiologyUniversity of AveiroAveiroPortugal CESAM-Centre for Environmental and Marine Studies-Department of Environment and Planning University of AveiroAveiroPortugal Erasmus University College Life Sciences DepartmentRotterdamthe Netherlands Institute of Biosciences PPGBANDepartment of ZoologyUniversidade Federal do Rio Grande do SulBrazil BioLiving Association FrossosAlbergaria-a-VelhaPortugal

The stone marten(Martes foina)is a carnivorous mammal that often consumes fleshy fruits,thus potentially promoting seed *** present study was developed in Bussaco National Forest,central Portugal,and aimed to assess the potential role of the stone marten in dispersing native and exotic plants in different forest landscape *** from stone marten scats and fleshy fruits were collected monthly and were thereafter identified and sowed in a nursery,following a randomized experimental *** emergence was monitored *** linear models were used to test for differences in time and success of emergence between seeds from scats and fruits of 3 native species(Rubus ulmifolius,Arbutus unedo and Celtis australis)and 1 exotic plant species with invasive behavior(Prunus laurocerasus).Fruit consumption by the stone martens significantly increased and accelerated the germination of the native *** but had no effect on the other 2 native species or on the invasive *** suggested that stone martens contribute to gene flow and forest regeneration by dispersing native plant ***,although the germination was not enhanced in the invasive species,the preference of stone martens for these fruits may potentially contribute to the proliferation of *** study represents a contribution to better understanding the fauna and flora interactions,enabling for a more conscious and effective decision-making in forest management.

关键词： germination invasive species Martes foina Prunus laurocerasus relict climactic forest

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Clinical/Behavioral Monitoring of Rodents and Rabbits Undergoing Scientific Experiments

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Open Journal of Veterinary Medicine 2024年第5期14卷 91-109页

作者： Luiz Cesar Cavalcanti pereira Jhônata Willy Rocha Coelho Hyago da Silva Medeiros Elidio Rita de Cássia dos Passos Ferraz Da Silva João Gabriel Regis Sobral Bárbara Alves de Brito Soledade Leandro Thomaz Vilela Isabele Barbieri Dos Santos Institute of Science and Technology in Biomodels (ICTB) Oswaldo Cruz Foundation (FIOCRUZ) Rio de Janeiro Brazil Laboratory Animal Facility of the Carlos Chagas Pavilion of the Animal Experimentation Center (CEA) Oswaldo Cruz Institute (IOC) Oswaldo Cruz Foundation (FIOCRUZ) Rio de Janeiro Brazil

Background: We currently have international and national guidelines regarding the assessment and monitoring of clinical signs and humane endpoints in animals used in teaching and research, which make the performance of these activities mandatory for any experiment and professional working in this area. Assigning the severity of a research experiment is the result of an analysis of records of observations of the animal’s behavior, and clinical signs. The aim of this study was to describe the importance of carrying out a severity assessment associated with clinical and behavioral monitoring of rodents and rabbits during experimentation to maintain the welfare of these animals undergoing scientific research. Methods: The literature search was carried out using the following terms: “Monitoring”;“Humane endpoints”;“Animal welfare”, “Rodents”;“Rabbits”, and as connectors “and”;“or”, in the following databases: PubMed;LILACS/BIREME and SciELO. Results: A total of 987 articles were identified in the databases, and 20 of these studies were included in this review. Conclusions: Humane endpoint protocols and procedure severity tables are of the utmost importance, both from an ethical point and to refine the results of research conducted on laboratory animals. They should be drawn up jointly by the teams responsible for the project and the maintenance of the animals during the research period, and the data obtained should be published so that the scientific community can have access to it, helping to disseminate these practices, as well as helping to draw up new procedures. Monitoring and evaluating the welfare and clinical condition of animals undergoing scientific research procedures is the responsibility of the professors, researchers, veterinarians, and animal facility coordinators. The Ethics Committee on the Use of Animals must monitor all the activities conducted with the animals, by inspecting the experimental procedures and the physical environment of the laboratory an

关键词： Monitoring Humane Endpoints Animal Welfare Experimental Design Laboratory Animals’ Investigations

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Role of Helicobacter pylori in gastric mucosa-associated lymphoid tissue lymphomas

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World Journal of Gastroenterology 2014年第3期20卷 684-698页

作者： Marta-Isabel pereira José Augusto Medeiros Clinical Hematology Department Coimbra University Hospital Center Institute of Physiology Faculty of MedicineUniversity of CoimbraAzinhaga de Santa Comba

Mucosa-associated lymphoid tissue (MALT) lymphoma is an indolent extranodal marginal zone B-cell lymphoma, originating in acquired MALT that is induced in mucosal barriers as part of a normal adaptive immune response to a chronic immunoinflammatory stimulus, most notably chronic infection by Helicobacter pylori (H. pylori). This antigenic stimulation initially leads to lymphoid hyperplasia; the acquisition of additional genetic aberrations culminates in the activation of intracellular survival pathways, with disease progression due to proliferation and resistance to apoptosis, and the emergence of a malignant clone. There are descriptions of MALT lymphomas affecting practically every organ and system, with a marked geographic variability partially attributable to the epidemiology of the underlying risk factors; nevertheless, the digestive system (and predominantly the stomach) is the most frequently involved location, reflecting the gastrointestinal tract’s unique characteristics of contact with foreign antigens, high mucosal permeability, large extension and intrinsic lymphoid system. While early-stage gastric MALT lymphoma can frequently regress after the therapeutic reversal of the chronic immune stimulus through antibiotic eradication of H. pylori infection, the presence of immortalizing genetic abnormalities, of advanced disease or of eradication-refractoriness requires a more aggressive approach which is, presently, not consensual. The fact that MALT lymphomas are rare neoplasms, with a worldwide incidence of 1-1.5 cases per 105 population, per year, limits the ease of accrual of representative series of patients for robust clinical trials that could sustain informed evidence-based therapeutic decisions to optimize the quality of patient care.

关键词： Mucosa-associated lymphoid tissue lymphoma Marginal zone lymphoma Helicobacter pylori Gastric lymphoma Eradication therapy Nuclear factor-kappa B pathway

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Fluidization of binary mixtures of sisal residue and sand:A new model for deriving the final fluidization velocity

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Particuology 2018年第5期16卷 10-22页

作者： David Vasconcelos Gabriele Batalha Luis Gabriel pereira Carlos Augusto Pires Chemical Reaction Engineering Laboratory Federal University of Bahia40.210-910SalvadorBABrazil

The influence of different factors on the fiuidization of a binary mixture of sisal residue and sand was *** particle sizes of the sand and sisal residue were varied from 0.2to 0.8mm and the biomass mass fractions from 2% to 9%.Some segregation was noted,and a significant relationship was found among the final fluidization velocity (Uff),the biomass and sand sizes,and the biomass mass fraction.A novel model was developed for predicting Uff,leading to an average discrepancy of 12.69% between the measured and predicted Uff compared with the best match of 15.32% when using a model from a previous *** new model was applied to data from studies using other biomass and a broad range of particle *** average divergences from measured values when using the new model were 7.9% for corn cob and walnut shell,and 20.5% for sweet sorghum bagasse,tobacco residue, and soy *** were superior to the values derived using other *** results confirm the accuracy of the model developed in this work and show that it represents a viable alternative way to calculate Uff for a binary mixture of sand and biomass.

关键词： Fluidization Binary mixture Sisal residue Final fluidization velocity model

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Production of bio-fertilizer from Ascophyllum nodosum and Sargassum muticum (Phaeophyceae)

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Journal of Oceanology and Limnology 2019年第3期37卷 918-927页

作者： SILVA Luís Daniel BAHCEVANDZIEV Kiril pereira Leonel Faculty of Science and Technology University of CoimbraCoimbra 3000-456Portugal Polytechnic Institute of Coimbra Agricultural CollegeⅡA-Institute of Applied ResearchCERNAS-Research Centre for Natural ResourcesEnvironmentand SocietyBencantaCoimbra 3045-601Portugal Faculty of Science and Technology University of CoimbraCoimbra 3000-548Portugal

Oceans and seas form a large body of water that contains a natural *** humans,represents a resource,which makes this a point of interest,from researches to improve the *** produce many compounds and secondary metabolites that can be used in different fi elds of industry such as food,agricultural,pharmaceutical and *** though seaweeds are ancestral resources,recently it was notorious a global interest in knowing more about its potentials,where biotechnology plays an important role in *** showed that seaweed has many bioactive compounds benefi cial to plant development,giving them a great potential as an agricultural *** seaweeds to the soil provides organic matter,minerals,trace elements,growth plant regulator,metabolites,vitamins,and amino acids and it can work as a soil *** Portugal,the use of seaweeds for agriculture is important since long time *** the past,populations that lived near coastal zone depended on the seaweeds as a family subsistence but,throughout the years,synthetic fertilizers replaced *** work aimed to assess the potential of the extracts obtained from Ascophyllum nodosum and from Sargassum muticum as an agricultural *** evaluation was carried out with rice plants(Oryza sativa)and lettuce(Lactuca sativa),in germination bioassays,the culture of rice and lettuce plants in pots,and culture of lettuce plants in *** that,seaweed liquid extracts were used in different concentrations in different *** show that extracts obtained from two seaweeds,*** and ***,can be promissory plant biofertilizer at a concentration of 25% and had a positive effect on seed germination,plant development,and production.

关键词： fertilizer bioactive compounds Ascophyllum nodosum Sargassum muticum Oryza sativa Lactuca sativa

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The effect of salinity on Fucus ceranoides (Ochrophyta,Phaeophyceae) in the Mondego River(Portugal)

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Journal of Oceanology and Limnology 2019年第3期37卷 881-891页

作者： COTAS Joao FIGUEIRINHA Artur pereira Leonel BATISTA Teresa Department of Life Sciences University of CoimbraCoimbra 3001-456Portugal MARE-Marine and Environmental Sciences Centre Faculty of Science and TechnologyUniversity of CoimbraCoimbra 3000-548Portugal Laboratory of Pharmacognosy Faculty of PharmacyUniversity of CoimbraCoimbra 3000-548Portugal LAQV REQUIMTEFaculty of PharmacyUniversity of CoimbraCoimbra 3000-548Portugal CIEPQPF Department of Chemical EngineeringFaculty of Science and TechnologyUniversity of CoimbraCoimbra 3030-790Portugal

Algae(and their extracts)are increasingly important for pharmaceutical applications due to the diversity of useful compounds they *** genus Fucus contains one of the most studied species,Fucus *** species *** differs from the others of the genus by presenting longitudinal air-vesicles and a capacity to survive at low *** is an alga that inhabits the Mondego River estuary(Portugal),at the southern limit of its distribution,and can serve as a role model to understand the effect of a salt gradient on the production of bioactive *** assessed the phenolic content and antioxidant activity of different *** extracts(***,aqueous and polysaccharide)prepared from samples harvested from two different zones to evaluate if the adaptation of *** to different salinity levels influenced its chemical *** antioxidant activity of the extracts was determined using 1,2-diphenyl-picrylhydrazyl(DPPH)and 2.2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)*** assays demonstrated that the methanolic extract of lyophilized *** that grew at low salinities was the most bioactive,***(IC 50=50.39μg/mL)and ABTS(TEAC=2.42).The total phenolic content(Folin-Ciocalteu method)and the methanolic extract of the lyophilized *** collected from a low salinity habitat exhibited the highest phenolic content(PGE=49.48μg/mg of lyophilized extract)amongst those *** layer chromatography(TLC)and Fourier transform infrared spectroscopy(FTIR)were used for the identification of compounds in the *** characterization allowed confirmation that the various extracts contained almost the same compounds but with notable quantitative *** on these results,we conclude that there were differences in the quantity of the compounds due to the effect of *** drying methods used were also found to have influenced the quality of the extracted compounds.

关键词： Fucus ceranoides salinity gradient sequential extractions phenolic content dependent bioactivities spectroscopic analysis

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Major regulatory mechanisms involved in sperm motility

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Asian Journal of Andrology 2017年第1期19卷 5-14页

作者： Rute pereira Rosalia Sa Alberto Barros Mario Sousa Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar University of Porto (ICBAS-UP) Rua Jorge Viterbo Ferreira 2284050-313 Porto Portugal and Multidisciplinary Unit for Biomedical Research-UMIB ICBAS-UP Centre for Reproductive Genetics Alberto Barros Av. do Bessa 2401° Dto. Frente 4100-012 Porto Portugal Department of Genetics Faculty of Medicine University of Porto. Alameda Prof. Hernani Monteiro 4200-319 Porto Portugal and Institute of Health Research an Innovation (13S) University of Porto Portugal.

The genetic bases and molecular mechanisms involved in the assembly and function of the flagellum components as well as in the regulation of the flagellar movement are not fully understood, especially in humans. There are several causes for sperm immotility, of which some can be avoided and corrected, whereas other are related to genetic defects and deserve full investigation to give a diagnosis to patients. This review was performed after an extensive literature search on the online databases PubMed, ScienceDirect, and Web of Science. Here, we review the involvement of regulatory pathways responsible for sperm motility, indicating possible causes for sperm immotility. These included the calcium pathway, the cAMP-dependent protein kinase pathway, the importance of kinases and phosphatases, the function of reactive oxygen species, and how the regulation of cell volume and osmolarity are also fundamental components. We then discuss main gene defects associated with specific morphological abnormalities. Finally, we slightly discuss some preventive and treatments approaches to avoid development of conditions that are associated with unspecified sperm immotility. We believe that in the near future, with the development of more powerful techniques, the genetic causes of sperm immotility and the regulatory mechanisms of sperm motility will be better understand, thus enabling to perform a full diagnosis and uncover new therapies.

关键词： antioxidants calcium membrane channels protein kinases sperm genetic abnormalities sperm motility

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Synthesis and functionalization of carbon xerogels to be used as supports for fuel cell catalysts

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Journal of Energy Chemistry 2013年第2期22卷 195-201页

作者： Jos L. Figueiredo Manuel F. R. pereira Laboratory of Catalysis and Materials Associate Laboratory LSRE/LCM Faculdade de Engenharia Universidade do Porto Rua Dr. Roberto Frias 4200-465 Porto Portugal

The synthesis and properties of carbon xerogels are briefly described in this mini-review, emphasizing the methods used for tuning their surface chemistry and textural properties in order to design efficient electrocatalysts for fuel cells. In particular, the role played by the surface functional groups in determining the loading, dispersion, oxidation state and stability of the metal phases is addressed.

关键词： carbon xerogels fuel cells electrocatalysts surface chemistry catalyst supports

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Iron toxicity resistance strategies in tropical grasses:The role of apoplastic radicular barriers

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Journal of Environmental Sciences 2019年第4期31卷 257-266页

作者： Advanio Inacio Siqueira-Silva Camilla Oliveira Rios Eduardo Gusmao pereira Institute of Biological and Health Sciences Federal University of Vicosa (UFV)Campus Florestal Minas Gerais Brazil Federal University of Western Para (UFOPA) University Campus of JurutiPara Brazil Postgradute Program in Management and Conservation of Natural and Agrarian Ecosystems UFV Campus Florestal Florestal Minas Gerais Brazil

The revegetation of mined areas poses a great challenge to the iron ore mining *** initial recovery process in degraded areas might rely on the use of Fe-resistant *** grasses, such as Paspalum densum and Echinochloa crus-galli, show different resistance strategies to iron toxicity; however, these mechanisms are poorly *** Fe-resistance mechanisms and direct iron toxicity as a function of root apex removal were investigated. To achieve this purpose, both grass species were grown for up to 480 hr in a nutrient solution containing 0.019 or 7 mmol/L Fe-EDTA after the root apices had been removed or maintained. Cultivation in the presence of excess iron-induced leaf bronzing and the formation of iron plaque on the root surfaces of both grass species, but was more significant on those plants whose root apex had been removed. Iron accumulation was higher in the roots, but reached phytotoxic levels in the aerial parts as well. It did not hinder the biosynthesis of chloroplastidic pigments. No significant changes in gas exchange and chlorophyll a fluorescence occurred in either grass when their roots were kept intact; the contrary was true for plants with excised root apices. In both studied grasses, the root apoplastic barriers had an important function in the restriction of iron translocation from the root to the aerial plant parts, especially in E. crus-galli. Root apex removal negatively influenced the iron toxicity resistance mechanisms(tolerance in P. densum and avoidance in E. crus-galli).

关键词： Paspalum densum Echinochloa crus-galli Apoplastic barrier Iron toxicity

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Caring to Teach: Exploring the Affective Economies of English Teachers in Singapore

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Chinese Journal of Applied Linguistics 2018年第4期41卷 488-505,552页

作者： Andrew J.pereira Nanyang Technological University National Institute of Education(NTU/NIE)Singapore

The ethical project of education hinges on the ideal of caring relations between teachers and students, an ideal that entails deep emotional commitments on the part of teachers. Drawing on interview data from a larger study of teachers’ lived experiences in Singapore’s secondary schools,this paper examines the cultural politics of caring as an emotional practice in teaching. The ethic of care serves to construct normative accounts of good teaching based on "feeling rules," and becomes a disciplinary technology for evaluating the professional, social and emotional competencies of teachers. I suggest that this project in turn entails an ideological effort to mobilize teachers’ emotional attachment to this ethical ideal. The ethic of care shapes the subjectivities, beliefs, and practices of English teachers, particularly as they circulate through the neoliberal imperatives of educational accountability regimes.

关键词： neoliberalism governmentality caring emotions affect cultural politics

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