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电离辐射诱导椎间盘纤维环细胞衰老的研究

中国矫形外科杂志 2024年第20期 1875-1881页

作者：李佳福钟炯彪 nam Vo 孟旭东杨帆彭家睿湖南师范大学附属岳阳医院(岳阳市人民医院)骨科美国匹兹堡大学骨科

[目的]探讨电离辐射对大鼠纤维环细胞衰老及衰老相关分泌表型表达的影响。[方法]选取6只成熟SD大鼠，取其腰尾段椎间盘纤维环细胞做体外细胞培养，将同等条件下培养的纤维环细胞随机分为两组，分别接受剂量为0 Gy、15 Gy的X线电离辐射... 详细信息

[目的]探讨电离辐射对大鼠纤维环细胞衰老及衰老相关分泌表型表达的影响。[方法]选取6只成熟SD大鼠，取其腰尾段椎间盘纤维环细胞做体外细胞培养，将同等条件下培养的纤维环细胞随机分为两组，分别接受剂量为0 Gy、15 Gy的X线电离辐射，检测两组纤维环细胞的衰老情况以及细胞衰老相关分泌表型（senescence-associated secretory phenotype, SASP）的表达。[结果]经15 Gy电离辐射处理后，纤维环细胞在形态学上表现出典型的衰老特征，SA-β-Gal测定显示纤维环细胞阳性染色百分比为77%。CCK-8实验显示纤维环细胞9 d内，未辐射组的OD值逐渐增加（P0.05）。IF及PCR检测均显示，辐射组衰老相关基因，包括P16 [(5.2±0.3) vs (1.0±0.1), P<0.001]、P21 [(12.9±0.5) vs (1.0±0.1), P<0.001]的mRNA相对表达量均显著高于未辐射组。此外，辐射组SASP的表达，包括胶原蛋白Col-1 [(1.6±0.1) vs (1.0±0.1), P=0.004]、Col-2 [(7.0±0.4) vs (1.0±0.1), P<0.001]、白细胞介素（interleukin, IL）-6 [(7.9±0.2) vs (1.0±0.1), P<0.001]、IL-8 [(1.8±0.1) vs (1.0±0.1), P<0.001]、基质金属蛋白酶（matrix metalloproteinase, MMP）-1 [(39.2±0.6) vs (1.0±0.1), P<0.001]、MMP-3 [(4.5±0.3) vs (1.0±0.1), P<0.001]的相对表达量均显著高于未辐射组。[结论]电离辐射能诱导大鼠椎间盘纤维环细胞衰老，其衰老相关分泌表型的表达明显增加。

关键词：椎间盘退变大鼠纤维环细胞电离辐射细胞衰老

Selective inhibition of cell growth by activin in SNU-16 cells

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World Journal of Gastroenterology 2006年第19期12卷 3000-3005页

作者： Young Il Kim Hee Joo Lee Inkoo Khang Byung-nam Cho Ha kyu Lee East-West Medical Research Institute kyung Hee University SeoulSouth Korea Department of Laboratory Medicine kyung Hee University College of MedicineSeoulSouth Korea School of Biological Sciences Seoul National University SeoulSouth Korea Department of Life Sciences The Catholic University of Korea PuchonSouth Korea

AIM： To investigate whether activin regulates the cell proliferation of human gastric cancer cell line SNU-16 through the mRNA changes in activin receptors, Smads and p21^CIP1/WAF1. METHODS： The human gastric cancer cell lines were cultured, RNAs were purified, and RT-PCRs were carried out with specifically designed primer for each gene. Among them, the two cell lines SNU-5 and SNU-16 were cultured with activin A for 24, 48 and 72 h. The cell proliferation was measured by MTT assay. For SNU-16, changes in ActRIA, ActRIB, ActRIIA, ActRIIB, Smad2, Smad4, Smad7, and p21^CIP1/WAF1 mRNAs were detected with RT-PCR after the cells were cultured with activin A for 24, 48 and 72 h. RESULTS： The proliferation of SNU-16 cells was down regulated by activin A whereas other cells showed no change. Basal level of inhibin/activin subunits, activin receptors, Smads, and p21^CIP1/WAF1 except for activin βB mRNAs was observed to have differential expression patterns in the human gastric cancer cell lines, AGS, KATO III, SNU-1, SNU-5, SNU-16, SNU-484, SNU-601, SNU-638, SNU-668, and SNU-719. Interestingly, significantly higher expressions of ActR IIA and IIB mRNAs were observed in SNU-16 cells when compared to other cells. After activin treatment, ActR IA, IB, and IIA mRNA levels were decreased whereas ActR IIB mRNA level increased in SNU-16 cells. Smad4 mRNA increased for up to 48 h whereas Smad7 mRNA increased sharply at 24 h and returned to the initial level at 48 h in SNU-16 cells. In addition, expression of the p21^CIP1/WAF1 the mitotic inhibitor, peaked at 72 h after activin treatment in SNU-16 cells. CONCLUSION： Our results suggest that inhibition of cell growth by activin is regulated by the negative feedback effect of Smad7 on the activin signaling pathway, and is mediated through p21^CIP1/WAF1 activation in SNU-16 cells.

关键词： Human gastric cancer cell lines, Activin A Cell proliferation Activin receptors Smads p21^CIP1/WAF1

Machine Learning for Detecting Blood Transfusion Needs Using Biosignals

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Computer Systems Science & Engineering 2023年第8期46卷 2369-2381页

作者： Hoon Ko chul Park Wu Seong Kang Yunyoung nam Dukyong Yoon Jinseok Lee Department of Biomedical Engineering College of Electronics and InformationKyung Hee UniversityYongin17104Korea Department of Internal Medicine Wonkwang University School of MedicineIksan54538Korea Department of Trauma Surgery Jeju Regional Trauma CenterCheju Halla General HospitalJeju63127Korea Department of Computer Science and Engineering Soonchunhyang UniversityAsan31538Korea Department of Biomedical Systems Informatics Yonsei University College of MedicineYongin03722Korea

Adequate oxygen in red blood cells carrying through the body to the heart and brain is important to maintain *** those patients requiring blood,blood transfusion is a common procedure in which donated blood or blood components are given through an intravenous ***,detecting the need for blood transfusion is time-consuming and sometimes not easily diagnosed,such as internal *** study considered physiological signals such as electrocardiogram(ECG),photoplethysmogram(PPG),blood pressure,oxygen saturation(SpO2),and respiration,and proposed the machine learning model to detect the need for blood transfusion *** the model,this study extracted 14 features from the physiological signals and used an ensemble approach combining extreme gradient boosting and random *** model was evaluated by a stratified five-fold crossvalidation:the detection accuracy and area under the receiver operating characteristics were 92.7%and 0.977,respectively.

关键词： Blood transfusion ECG PPG pulse transit time blood pressure machine learning

Wire-Shaped 3D-Hybrid Supercapacitors as Substitutes for Batteries

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Nano-Micro Letters 2020年第2期12卷 211-223页

作者： Kyeong‑nam Kang Ananthakumar Ramadoss Jin‑Wook Min Jong‑chul Yoon Deokjung Lee Seok Ju Kang Ji‑Hyun Jang School of Energy and Chemical Engineering Low Dimensional Carbon Materials CenterUlsan National Institute of Science and TechnologyUlsan 44919Republic of Korea Laboratory for Advanced Research in Polymeric Materials Central Institute of Plastic Engineering and TechnologyBhubaneswar 751024India School of Mechanical Aerospace and Nuclear EngineeringUlsan National Institute of Science and TechnologyUlsan 44919Republic of Korea

We report a wire-shaped three-dimensional(3D)-hybrid supercapacitor with high volumetric capacitance and high energy density due to an interconnected 3D-configuration of the electrode allowing for large number of electrochemical active sites,easy access of electrolyte ions,and facile charge transport for flexible wearable *** interconnected and compact electrode delivers a high volumetric capacitance(gravimetric capacitance)of 73 F cm−3(2446 F g−1),excellent rate capability,and cycle *** 3D-nickel cobalt-layered double hydroxide onto 3D-nickel wire(NiCo LDH/3D-Ni)//the 3D-manganese oxide onto 3D-nickel wire(Mn3O4/3D-Ni)hybrid supercapacitor exhibits energy density of 153.3 Wh kg−1 and power density of 8810 W kg−*** red lightemitting diode powered by the as-prepared hybrid supercapacitor can operate for 80 min after being charged for tens of seconds and exhibit excellent electrochemical stability under various deformation *** results verify that such wire-shaped 3D-hybrid supercapacitors are promising alternatives for batteries with long charge–discharge times,for smart wearable and implantable devices.

关键词： Three-dimensional(3D)metal current collector Flexible hybrid supercapacitor Wire-shaped supercapacitor High energy density device Fast charging energy storage system

Effect of vehicle weight on natural frequencies of bridges measured from traffic-induced vibration

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Earthquake Engineering and Engineering Vibration 2003年第1期2卷 109-116页

作者： chul-Young Kim Dae-Sung Jung nam-Sik Kim Soon-Duck Kwon Maria Q.Feng Dept.of Civil & Envir.Eng. Myongji UniversityKorea Professor Dept.of Civil & Envir.Eng. Myongji UniversityKorea Research Associate Hyundai Institute of Construction Technology Hyundai E&C Co.Ltd.Korea Chief Researcher Dept.of Civil Engineering Chonbuk National UniversityKorea Professor Dept.of Civil & Envir.Eng. University of CaliforniaIrvineCaliforniaUSA Professor

Recently,ambient vibration test(AVT)is widely used tu estimate dynamic characteristics of large civil struc- *** characteristics ean be affected by various envirnnmental factors such as humidity,intensity of wind,and *** these environmental conditions,tire mass of vehicles may change the measured valnes when traffic-in- duced vibration is used as a source of AVT tor *** effect of vehicle mass on dynamic characteristics is investigated through traffic-induced vibration tests on three bridges;(1)three-span suspension bridge(128m+404m+128m),(2) five-span continuous steel box girder bridge(59m+3@ 95m+59m),(3)simply supported plate girder bridge(46m). Acceleration histories of each measurement location under normal traffic are recorded for 30 minutes at *** recor- ded histories are divided into individual vibrations and are combined into two groups aceording to the level of vibration;one by heavy vehicles such as trucks and buses and the other by light vehicles such as passenger *** processing of the two groups of signals shows that,for the middle and long-span bridges,the difference can be hardly detected,but,for the short span bridges whose mass is relatively small,the measured natural frequencies can change up to 5.4%.

关键词： ambient vibration test traffic induced vibration vehicle mass suspension bridge short-span bridge dynamic characteristics natural frequency

Thermo-Mechanical, Physico-Chemical, Morphological, and Fire Characteristics of Eco-Friendly Particleboard Manufactured with Phosphorylated Lignin Addition

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Journal of Renewable Materials 2024年第7期12卷 1311-1341页

作者： Apri Heri Iswanto Harisyah Manurung Asma Sohail Lee Seng Hua Petar Antov Deded Sarip Nawawi Sarah Latifah Dewi Shafa Kayla Sukma Surya Kusumah Muhammad Adly Rahandi Lubis Linda MakovickáOsvaldová MohdHazwan Hussin Rangabhashiyam Selvasembian Lum Wei Chen Puji Rahmawati Nurcahyani nam Hun Kim Widya Fatriasari Department of Forest Products Faculty of ForestryUniversitas Sumatera UtaraKampus USU Padang BulanMedanNorth Sumatra20155Indonesia Department of Chemistry Lahore College for Women UniversityLahore4444Pakistan Department of Wood Industry Faculty of Applied SciencesUniversiti Teknologi MARA(UiTM)Cawangan Pahang Kampus JengkaShah Alam26400Malaysia Faculty of Forest Industry University of ForestrySofia1797Bulgaria Department of Forest Product Faculty of Forestry and EnvironmentIPB UniversityBogor16680Indonesia Research Center for Biomass and Bioproducts National Research and Innovation Agency(BRIN)Cibinong16911Indonesia Department of Forest Engineering Faculty of Security EngineeringUniversity of ZilinaZilina01001Slovakia Materials Technology Research Group(MaTReC) School of Chemical SciencesUniversiti SainsMalaysiaMindenPenang11800Malaysia Department of Environmental Science and Engineering School of Engineering and SciencesSRM University-APAmaravatiAndhra Pradesh522240India Department of Bio and Natural Resources Faculty of Bioengineering and TechnologyUniversitiMalaysia KelantanJeliKelantan17600Malaysia Department of Forest Biomaterials Engineering College of Forest and Environmental SciencesKangwon National UniversityChuncheon24341Republic of Korea

Lignin,lignosulfonate,and synthesized phosphorylated lignosulfonate were introduced as greenﬁllers in citric acid-sucrose adhesives for bonding particleboard fabricated from areca leaf sheath(ALS).The characteristics of particleboards were compared to that of ultralow emitting formaldehyde(ULEF-UF).Theﬁllers derived from Eucalyptus ***-lignin were added forﬂame retardancy enhancement.10%of each lignin and modiﬁed lig-nin was added into the ULEF-UF and citric acid-sucrose bonded *** applied to particle-boards included thermal characteristics,X-ray diffraction analysis(XRD),morphological properties,Fourier transform infrared spectroscopy(FTIR),as well as physical,mechanical,andﬁre resistance characteristics of the laboratory-fabricated *** and modiﬁed lignin resulted in improved thermal stability of the composites bonded with ULEF-UF while the improvement in the particleboard bonded with citric acid-sucrose was not signiﬁ*** introduction ofﬁller exerted a higher inﬂuence on the UF-bonded particleboards compared to composites fabricated with citric ***,the presence of lignin,lignosulfonate,and phosphorylated lignosulfonate enhanced the mechanical strength of the ULEF-bonded particleboards,although their dimensional stability has ***,the use of lignin and lignosulfonate enhanced theﬁre resis-tance of the particleboards produced with lower observed weight *** laboratory particleboards exhibited satisfactoryﬁre resistance,attaining a V-0 rating in according to the UL-94 standard.

关键词： Modiﬁed lignin eco-friendly particleboard ﬁre and thermal properties citric acid-sucrose ultralow-emitting urea-formaldehyde resin

STUDY ON DRILLING CHARACTERISTICS AND MECHANICAL PROPERTIES OF CFRP COMPOSITES

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Acta Mechanica Solida Sinica 2008年第4期21卷 364-368页

作者： Seung-chul Lee Seong-Taek Jeong Jong-nam Park Sun-Jin Kim Gyu-Jae Cho Department of Mechanical Engineering Chosun UniversityGraduate SchoolGwangjuKorea Small Business Corporation GwangjuKorea Department of Computer Mechanic Chosun College of Science & TechnologyGwangjuKorea Department of Fire Safety Management Seokang CollegyGwangjuKorea Department of Mechanical Engineering Chosun UniversityGwangjuKorea

Stacking plates of CFRP composite materials are increasingly used because of their unique characteristics. However, unlike other materials used in metallurgy they have a disadvantage of uneven quality and anisotropy when combined with other composites. Hence, specimens of CFRP stacking plates are manufactured by changing orientation angles throughout three quasiisotropic plies （0°/45°/90°/-45°）68, （03°/453°/903°/-453°）2s, and （06°/456°/906°/-456°）s and throughout three cross plies （0°/90°/0°/90°）68, （03 °/903 °/03°/903°）2s, and （06 °/906 °/06°/906°）8 In this study 3-point bending tests and transverse bending tests have been carried out in order to find out mechanical characteristics according to orientation angles by stacking in 6 different types along with the change of stacking composition method of a CFRP composite.

关键词： composite materials stacking plates

Keystone design perforator island flap in facial defect reconstruction

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World Journal of Clinical Cases 2020年第10期8卷 1832-1847页

作者： Soo Yeon Lim Chi Sun Yoon Hyun Gun Lee kyu nam Kim Department of Plastic and Reconstructive Surgery Konyang University HospitalUniversity of Konyang College of MedicineMyunggok Medical Research CenterDaejeon 35365South Korea Department of Plastic and Reconstructive Surgery Wonkwang University HospitalWonkwang University School of MedicineIksan 54538South Korea

Facial defect coverage is a common subject in the field of reconstructive *** are many methods for facial defect reconstruction,and reconstructive surgeons should choose the most appropriate method on a case-by-case basis to achieve both functional and aesthetic *** various options for facial reconstruction,the local flap technique is considered the best reconstructive modality to provide good tissue matches of color and texture,which is consistent with the ideal goal of reconstruction(replacement of like-with-like).Keystone design perforator island flap(KDPIF),devised by Behan in 2003,has been applied to various fields of reconstructive surgery in the past decade due to its design simplicity,robust vascular supply,and *** studies have reported KDPIF reconstruction of facial defects,such as large parotid defects,small-to-moderate nasal defects,and eyelid ***,KDPIF has been used relatively less in facial defects than in other body regions,such as the trunk and *** purpose of this review is to provide an organized overview of facial KDPIF reconstruction including the classification of KDPIF,modifications,physiology,mechanism of flap movement,consideration of facial relaxed skin tension lines and aesthetics,surgical techniques,clinical applications,and precautions for successful execution of KDPIF reconstruction.

关键词： Keystone design perforator island flap Reconstructive surgery Facial defects Aesthetics Plastic surgery Flap surgery

Mutation of Oryza sativa CORONATINE INSENSITIVE 1b(OsCOI1b) delays leaf senescence

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Journal of Integrative Plant Biology 2015年第6期57卷 562-576页

作者： Sang-Hwa Lee Yasuhito Sakuraba Taeyoung Lee kyu-Won Kim Gynheung An Han Yong Lee nam-Chon Paek Department of Plant Science Plant Genomics and Breeding InstituteResearch Institute of Agriculture and Life SciencesSeoul National University Interdisciplinary Program in Bioinformatics Seoul National University Department of Plant Molecular Systems Biotechnology Crop Biotech InstituteKyung Hee University Department of Agricultural Biotechnology Seoul National University

Jasmonic acid （JA） functions in plant development, including senescence and immunity. Arabidopsis thaliana CORONATINE INSENSITIVE 1 encodes a JA receptor and functions in the JA‐responsive signaling pathway. The Arabidopsis genome harbors a single COI gene, but the rice （Oryza sativa） genome harbors three COI homologs, OsCOI1a, OsCOI1b, and OsCOI2. Thus, it remains unclear whether each OsCOI has distinct, additive, synergistic, or redundant func-tions in development. Here, we use the oscoi1b‐1 knockout mutants to show that OsCOI1b mainly affects leaf senescence under senescence‐promoting conditions. oscoi1b‐1 mutants stayed green during dark‐induced and natural senescence, with substantial retention of chlorophylls and photosyn-thetic capacity. Furthermore, several senescence‐associated genes were downregulated in oscoi1b‐1 mutants, including homologs of Arabidopsis thaliana ETHYLENE INSENSITIVE 3 and ORESARA 1, important regulators of leaf senescence. These results suggest that crosstalk between JA signaling and ethylene signaling affects leaf senescence. The Arabidopsis coi1‐1 plants containing 35S：OsCOI1a or 35S：OsCOI1b rescued the delayed leaf senescence during dark incubation, sug-gesting that both OsCOI1a and OsCOI1b are required for promoting leaf senescence in rice. oscoi1b‐1 mutants showed significant decreases in spikelet fertility and grain weight, leading to severe reduction of grain yield, indicating that OsCOI1‐mediated JA signaling affects spikelet fertility and grain filling.

关键词： Jasmonic acid leaf senescence OsCOI1b rice stay-green