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Proteome analysis of alfalfa roots in response to water deficit stress

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Journal of Integrative Agriculture 2016年第6期15卷 1275-1285页

作者： Rahman Md Atikur kim Yong-Goo Alam Iftekhar LIU Gong-she Lee Hyoshin Lee Jeung Joo Lee Byung-Hyun Division of Applied Life Sciences (BK21Plus) Institute of Agriculture & Life Sciences (IALS) Gyeongsang National University Jinju 660-701 Republic of Korea Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China National Institute of Biotechnology Dhaka-1349 Bangladesh Division of Forest Biotechnology Korea Forest Research Institute Suwon 441-847 Republic of Korea Department of Applied Biology Institute of Agriculture & Life Sciences (IALS) Gyeongsang National University Jinju 660-701 Republic of Korea

To evaluate the response of alfalfa to water deficit （WD） stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H202 content, lipid peroxidation, DPPH （1,1-diphenyl-2-picrylhydrazyl）-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis （2-DE）. A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase （APx2） and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid （ABA） biosynthesis, reactive oxygen species （ROS） regulation, transcription/translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.

关键词： alfalfa proteome root water deficit stress

Preliminary Evaluation for Comparative Antioxidant Activity in the Water and Ethanol Extracts of Dried Citrus Fruit (Citrus unshiu) Peel Using Chemical and Biochemical in VitroAssays

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Food and Nutrition Sciences 2013年第2期4卷 177-188页

作者： Joo-Shin kim Department of Convergence Industry Health Industry Program The Seoul Venture University Seoul South Korea.

Comparative antioxidant activities of the water and ethanol extracts obtained from dried citrus fruit (Citrus unshiu) peel were determined using chemical and biochemical in vitro assays. Chemical assays were used for evaluation of 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, hydrogen peroxide scavenging, and reducing power of both extracts and their total phenolic, flavonoid and tannin contents and antioxidant activities were investigated. Biochemical assays were performed to evaluate the inhibition activities of AAPH-induced rat RBC hemolysis and Fe2+-induced lipid peroxidation using rat brain tissue cells. Linear correlation between the antioxidant activities of both extracts were determined by chemical assays, and total phenolic, flavonoid and tannin contents was observed in concentration-dependent mode. Both extracts showed >95% DPPH radical scavenging and >85% hydrogen peroxide scavenging, and higher reducing capacity at the same level of 3200 ppm. In the inhibition activity of AAPH-induced hemolysis, water extracts showed a strong activity in concentration-dependent mode up to 1600 ppm with no statistical difference found between 1600 and 3200 ppm (P > 0.05). In the inhibition activity of Fe2+-induced lipid peroxidation, ethanol extracts showed the higher inhibition percentage of lipid peroxidation than those of water extracts at the same concentration with no significant difference (P > 0.05) found in the range of 800 to 3200 ppm. The extracts of dried Citrus unshiu peel may be considered as potential antioxidant ingredients of functional food depending on the conditions at which reactive oxygen species are implicated.

关键词： Antioxidant Citrus unshiu Peel Total Phenolics Flavonoid Tannin

Engineering drought and salinity tolerance traits in crops through CRISPR-mediated genome editing:Targets,tools,challenges,and perspectives

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Plant Communications 2022年第6期3卷 208-234页

作者： Rahul Mahadev Shelake Ulhas Sopanrao Kadam Ritesh Kumar Dibyajyoti Pramanik Anil Kumar Singh Jae-Yean kim Division of Applied Life Science(BK21 Four Program) Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityJinju 52828Korea Department of Agronomy&Plant Genetics University of MinnesotaSaint PaulMN 55108USA ICAR-National Institute for Plant Biotechnology LBS CentrePusa CampusNew Delhi 110012India Division of Life Science Gyeongsang National University501 Jinju-daeroJinju 52828Korea

Prolonged periods of drought triggered by climate change hamper plant growth and cause substantial agricultural yield losses every *** addition to drought,salinity is one of the major abiotic stresses that severely affect crop health and agricultural *** responses to drought and salinity involve multiple processes that operate in a spatiotemporal manner,such as stress sensing,perception,epigenetic modifications,transcription,post-transcriptional processing,translation,and post-translational ***,drought and salinity stress tolerance are polygenic traits influenced by genomeenvironment *** of the ideal solutions to these challenges is the development of highyielding crop varieties with enhanced stress tolerance,together with improved agricultural ***,genome-editing technologies,especially clustered regularly interspaced short palindromic repeats(CRISPR)tools,have been effectively applied to elucidate how plants deal with drought and saline *** this work,we aim to portray that the combined use of CRISPR-based genome engineering tools and modern genomic-assisted breeding approaches are gaining momentum in identifying genetic determinants of complex traits for crop *** review provides a synopsis of plant responses to drought and salinity stresses at the morphological,physiological,and molecular *** also highlight recent advances in CRISPR-based tools and their use in understanding the multi-level nature of plant adaptations to drought and salinity *** CRISPR tools with modern breeding approaches is ideal for identifying genetic factors that regulate plant stress-response pathways and for the introgression of beneficial traits to develop stress-resilient crops.

关键词： CRISPR/Cas drought tolerance genome editing polygenic traits salt tolerance trait introgression

Weldability evaluation and tensile strength estimation model for aluminum alloy lap joint welding using hybrid system with laser and scanner head

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中国有色金属学会会刊：英文版 2012年第S3期22卷 596-604页

作者： Dong-Yoon kim Young-Whan PARK Department of Mechanical Engineering Pukyong National University

Aluminum welding using a hybrid system with a laser and scanner welding head was performed under various welding conditions to verify the feasibility of applying an aluminum alloy to a car *** experimental material was 5J32 aluminum alloy,and the laser power,welding speed,and laser incidence angle were used as the control *** weld bead shape and the tensile shear strength were evaluated in order to understand the aluminum lap joint weld *** of variance (ANOVA) was conducted to identify the effect of the process variables on the tensile shear *** strength estimation models using three different regression models were also *** input variables were the laser power,welding speed,and laser incidence angle,and the output was the tensile shear *** the models,the second-order polynomial estimation model had the best estimation performance,and the average error rate of this model was 0.058.

关键词： laser welding scanner remote welding 5J32 aluminum alloy regression model

It’s People That Make Places

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China Today 2011年第1期60卷 62-64页

作者： kim ROTT an English teacher-turned-librarian Sentinel High School in Missoula Montana

ON July 9, 2010, I paused on the Great Wall of China on the outskirts of Beijing, trying to imagine all the people who had stood in that spot before, feeling the same breeze and viewing the same horizon.

关键词：旅游经济旅游风光旅游景点旅游市场

Endoscopic treatment for gastrointestinal stromal tumor: Advantages and hurdles

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World Journal of Gastrointestinal Endoscopy 2015年第3期7卷 192-205页

作者： Hyung Hun kim Department of Internal Medicine the Catholic University of Korea College of Medicine

One of the most prominent characteristics of gastrointestinal stromal tumors(GISTs) is their unpredictable and variable behavior. GISTs are not classified as "benign" or "malignant" but are rather stratified by their associated clinical risk of malignancy as determined by tumor size, location, and number of mitoses identified during surgicalhistology. The difficulty in assessing the malignant potential and prognoses of GISTs as well as the increasing incidence of "incidental GISTs" presents challenges to gastroenterologists. Recently, endoscopic enucleation has been actively performed as both a diagnostic and therapeutic intervention for GISTs. Endoscopic enucleation has several advantages, including keeping the stomach intact after the removal of GISTs, a relatively short hospital stay, a conscious sedation procedure, relatively low cost, and fewer human resources required compared with surgery. However, a low complete resection rate and the risk of perforation could reduce the overall advantages of this procedure. Endoscopic full-thickness resection appears to achieve a very high R0 resection rate. However, this technique absolutely requires a very skilled operator. Moreover, there is a risk of peritoneal seeding due to large active perforation. Laparoscopy endoscopy collaborations have been applied for more stable and pathologically acceptable management. These collaborative procedures have produced excellent outcomes. Many procedures have been developed and attempted because they were technically possible. However, we should first consider the theoretical basis for each technique. Until the efficacy and safety of sole endoscopic access are proved, the laparoscopy endoscopy collaborative procedure appears to be an appropriate method for minimally destructive GIST surgery.

关键词： Gastrointestinal stromal tumor Endoscopy Laparoscopy Efficacy Safety

Study of Numerical Analysis on Smoke Exhaust Performance of Portable Smoke Exhaust Fan

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Open Journal of Fluid Dynamics 2017年第2期7卷 205-218页

作者： Jung-Yup kim Multi Disaster Countermeasures Organization Korea Institute of Civil Engineering and Building Technology Goyang Korea

In houses and general buildings of environment where smoke control equipment is not installed, or its operation is difficult, there is a need to develop and utilize a portable smoke exhaust fan that can perform emergency ventilation in case of fire suppression and rescue operations. As a part of the development process for portable smoke exhaust fans, a numerical analysis model of the portable smoke exhaust fan was derived from the building in which a fire occurred, and an analysis on the smoke and toxic gas exhaust effects was performed in this study. The numerical analysis results showed that under a condition where the height of an opening is less than 1.0 m after fire source disappears in a situation where the smoke exhaust fan does not operate, smoke does not come out well, and thus the density of smoke within the target area does not drop significantly. On the other hand, if the smoke exhaust fan operates after the fire source disappears, smoke is directly exhausted to the outside due to the operation of the smoke exhaust fan, and the smoke density within the space drops sharply in all opening height conditions, thereby getting close to zero.

关键词： Smoke Exhaust Portable Fan Smoke Density CO Density Numerical Analysis

ESTABLISHMENT OF ASSESSMENT SYSTEM FOR STRUCTURAL FLOOD CONTROL MEASURES

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Tropical Cyclone Research and Review 2019年第1期8卷 46-52页

作者： CHO-RONG kim Department of Land Water and Environment Research Korea Institute of Civil Engineering and Building Technology

Due to abnormal weather environment such as global warming and greenhouse effect, the risk of flood has been increased with larger scale of flood damages. In this regard, it is needed to establish a comprehensive and integrated system to evaluate the most optimized measures for flood control through eliminating uncertainties of socio-economic impacts. Assessment System of Structural Flood Control Measures(ASFCM) was developed for determining investment priorities of the flood control measures and establishing the social infrastructure projects. ASFCM consists of three modules: 1) the initial setup and inputs module, 2) the flood and damage estimation module, and 3) the socio-economic analysis ***, the D/B for flood damage estimation was constructed, which is the initial and input data about the estimation unit, property, historical flood damages, and topographic and hydrological data in the target area were applied. After that, it is important to classify local characteristic for constructing flood damage data. Five local characteristics(big city, medium size city, small city, farming area, and mountain area) are classified by criterion of application(population density). Next step is the floodplain simulation with HEC-RAS which is selected to simulate inundation. Through inputting the D/B and damage estimation, it is able to estimate the total damage(only direct damage) that is the amount of cost to recover the socio-economic activities back to the safe level before flood did occur. The last module suggests the economic analysis index(B/C ratio) with Multidimensional Flood Damage. Consequently, ASFCM suggests the reference index in constructing flood control measures to reduce water-related damage and decision-makers can choose the proper flood control measures in the socioeconomic aspects.

关键词： ASFCM socio-economic assessment flood damage structural flood control HEC-RAS

Transmission of Avian Influenza Virus by Humpback Whale and Its Stranding along the Atlantic Coast with CO2Emissions

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Journal of Biomedical Science and Engineering 2018年第12期11卷 359-381页

作者： Tai-Jin kim Department of Chemical Engineering University of Suwon Hwaseong City South Korea

Humpback whales are migratory, spending summers in cooler, high-latitude waters and mating and calving in tropical and subtropical waters in 14 identified district population segments. It may be possible that the coastal areas are infected with low pathogenic avian influenza (LPAI) during the release of infected humpback whale feces. Therefore, humpback whales can be an effective reservoir of the avian influenza virus (AIV) from the Poles to the Continents to spread AIV to coastal animals. Strong ultraviolet (UV) exposure amidst CO2 emission increase and minimal sunspot number might cause mutations of aquatic virus and humpback whale in the Antarctic and the Arctic. LPAI or highly pathogenic avian influenza (HPAI) is expressed in the Continents under appropriate environmental factors. Since penguins are birds while humpback whales are marine mammals, the humpback whales infected by the mutant virus might cause interspecies transmission to a new host with evolutionary changes. The migration pattern is seasonally similar between migratory bird and humpback whale except: 1) different species of bird versus whale, 2) different landing area of land versus coast, 3) similar infection means of bird feces versus humpback whale feces. The contribution of AIV transmission by whales was several times larger than that by migratory birds. Therefore, the routes of humpback whales should be considered to prevent AIV outbreaks in addition to the flyways of migratory birds. Humpback whale stranding (y) along the Atlantic Coast of the USA was correlated with CO2 emissions (x) to have y = 0.3515x + 18.595 (R2 = 0.4069) during 1992-2016 while y = 0.0652x + 4.5847, (R2 = 0.6128) during 2016-2018. AIV outbreak in 2010 (y) along the Atlantic Coast was also correlated with humpback whale stranding (2016-2018) (x) as y = 0.1387x + 6.8184 (R2 = 0.3966). Since AIV outbreak was linearly (R2 = 0.9967) correlated with the minimum sunspot number, it was postulated that the unusual mortality event

关键词： Transmission Avian Influenza Virus Humpback Whale Stranding CO2 Emissions