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The host-targeting compound peruvoside has a broad-spectrum antiviral activity against positive-sense RNA viruses

Acta Pharmaceutica Sinica B 2023年第5期13卷 2039-2055页

作者： Kan Xing Wu Thinesshwary Yogarajah Marcus Wing choy Loe Parveen Kaur Regina Ching Hua Lee Chee Keng Mok Yi Hao Wong Patchara Phuektes Li Sze Yeo Vincent T.K.Chow Yong Wah Tan Justin Jang Hann Chu Department of Microbiology and Immunology Yong Loo Lin School of MedicineNational University Health SystemNational University of SingaporeSingapore 117597Singapore Department of Pathobiology Faculty of Veterinary MedicineKhonkaen UniversityKhonkaen 40002Thailand Infectious Disease Programme Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A∗STAR)Singapore 138648Singapore Lee Kong Chian School of Medicine Nanyang Technological UniversitySingapore 308232Singapore NUSMed Biosafety Level 3 Core Facility Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117599Singapore

Positive-sense RNA viruses modify intracellular calcium stores,endoplasmic reticulum and Golgi apparatus(Golgi)to generate membranous replication organelles known as viral *** factories provide a conducive and substantial enclave for essential virus replication via concentrating necessary cellular factors and viral proteins in ***,we identified the vital role of a broadspectrum antiviral,peruvoside in limiting the formation of viral ***,we revealed the pleiotropic cellular effect of Src and PLC kinase signaling via cyclin-dependent kinase 1 signaling leads to Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1(GBF1)phosphorylation and Golgi vesiculation by peruvoside *** ramification of GBF1 phosphorylation fosters GBF1 deprivation consequentially activating downstream antiviral signaling by dampening viral factories *** investigation showed signaling of ERK1/2 pathway via cyclin-dependent kinase 1 activation leading to GBF1 phosphorylation at Threonine 1337(T1337).We also showed 100%of protection in peruvoside-treated mouse model with a significant reduction in viral titre and without measurable cytotoxicity in *** findings highlight the importance of dissecting the broad-spectrum antiviral therapeutics mechanism and pave the way for consideration of peruvoside,host-directed antivirals for positive-sense RNA virus-mediated disease,in the interim where no vaccine is available.

关键词： Positive-sense RNA viruses Peruvoside Antiviral CDKI signaling Viral factories Calcium efflux Broad-spectrum Golgi apparatus

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A 6-digit RSD Analog-to-Quaternary Converter with CMOS Current Mode Quaternary Adders

A 6-digit RSD Analog-to-Quaternary Converter with CMOS Curre...

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2007 7th International Conference on ASIC Electronic Version(ASICON 2007)

作者： Cheong-Fat Chan Chiu-Sing choy Kong-Pang Pun Department of Electronic Engineering The Chinese University of Hong KongShatinHong Kong

This paper presents a 6-digit Analog-to-Quaternary Converter（AQC） using a 0.35μm CMOS *** proposed CMOS AQC uses pipelined architecture with redundant signed digit（RSD） error correction algorithm.A CMOS quaternary adder is proposed to handle quaternary addition in the correction *** converter has simulated SNDR,SFDR and THD of 65.17dB,73.89dB and-73.26dB at 2.5V supply voltage and 50MHz sampling rate.

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腺苷延长肌瓣缺血耐受时间的实验研究

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中华整形烧伤外科杂志 1998年第5期14卷 363-366页

作者：赫伟钟安国章建荣 choy.Pang 中国医学科学院中国协和医科大学整形外科医院北京100041 加拿大多伦多大学儿童医院研究所

目的研究缺血前局部使用腺苷对猪背阔肌瓣缺血耐受的促进作用和机理。方法用不同剂量(0,0.5,2.0mg)的腺苷从腋动脉穿刺注入肌瓣,肌瓣缺血4,48小时后切取肌瓣,染色后测定肌瓣成活率,腺苷注射前、后,缺血后分别采取肌肉标本做三磷酸腺苷(A... 详细信息

目的研究缺血前局部使用腺苷对猪背阔肌瓣缺血耐受的促进作用和机理。方法用不同剂量(0,0.5,2.0mg)的腺苷从腋动脉穿刺注入肌瓣,肌瓣缺血4,48小时后切取肌瓣,染色后测定肌瓣成活率,腺苷注射前、后,缺血后分别采取肌肉标本做三磷酸腺苷(ATP)定量分析。结果腺苷注射组肌肉坏死率明显低于对照组(对照组:40.3％±2.2％),0.5mg 组:20.6％±1.6％,2mg 组:18.2％±1.0％)。腺苷注入组缺血后肌肉 ATP 水平明显高于对照组。结论缺血前局部使用小剂量腺苷对缺血肌瓣有保护作用,并伴随肌肉 ATP 分解水平的减低。

关键词：腺苷肌瓣缺血耐受时间肌移植

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Separation and determination of organic and inorgainc anions in human serum and urine samples by Capillary Zone Electrophoresis

Separation and determination of organic and inorgainc anions...

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第四届国际华夏学者分析化学研讨会

作者： Y.S.Fung Jacqueline T.S.choy Department of Chemistry The University of Hong Kong Pokfulam RoadHong Kong SARChina Department of Chemistry The University of Hong Kong Pokfulam RoadHong Kong SARChina Department of Chemistry The University of Hong Kong Pokfulam RoadHong Kong SARChina

＜正＞The determination of organic acids and inorganic anions in serum and urine provides important information for pathological diagnosis of inborn disorders. The current methods for the determination of organic acids are high performance liquid chromatography （HPLC） and gas chromatography-mass spectrometry （GC-MS）. The procedure requires lengthy sample preparation time and the need of skilled manpower and dedicated instrumentation. With rapid analysis and fast turn around analysis time, procedures based on capillary electrophoresis （CE） have been shown to provide a more suitable method for the identification of acidaemia. To develop a CE procedure capable of simultaneously analyzing a large group of organic, and inorganic anions with physiological interest in human serum and urine, a new buffer system （3mM BTA, 1.5mM TEPA and 15mM Tris） is developed with indirect UV detection at 210 nm upon careful mobility matching with target anions. Under the optimized conditions with pH adjusted to 8.4, a baseline separation is achieved for the following 9 anions （chloride, sulphate, oxalate, succinate, phosphate, carbonate, citrate, pyruvate, and lactate） in a typical serum sample and 10 anions in a urine sample （chloride, sulphate, oxalate, succinate, phosphate, carbonate, citrate, acetate, pyruvate and glucuronate） using an uncoated silica capillary column. Under hydrostatic sampling, working ranges from 0.005-2mM and detection limits from 0.5-4 mM are obtained with satisfactory repeatability for migration time （RSD=0.56-0.83%, n=5） and peak height （4.2-5.5%, n=5） measurement. The CE procedure developed has been applied for real sample analysis, with results showing good repeatability, low detection limit and wide working range. Factors affecting the working conditions and optimization of CE separation of organic, and inorganic anions in human serum and urine samples and the reliability and applicability of the analytical procedure developed will be discussed in the meeting.

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采用微阵列-比较基因组杂交进行产前诊断的局限性和困难

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中华医学遗传学杂志 2011年第1期28卷 47-51页

作者：陈瑛蔡光伟南京医科大学附属苏州医院生殖与遗传中苏州215002 香港中文大学妇产科学系

应用微阵列-比较基因组杂交（microarray comparative genomic hybridization，aCGH）技术检测基因拷贝数变异（copy number variation，CNV）可对染色体亚微结构异常进行定位，在研究领域和临床实验室中迅速成为一个了解基因和患者遗... 详细信息

应用微阵列-比较基因组杂交（microarray comparative genomic hybridization，aCGH）技术检测基因拷贝数变异（copy number variation，CNV）可对染色体亚微结构异常进行定位，在研究领域和临床实验室中迅速成为一个了解基因和患者遗传病因的有力的工具。由于它在产前诊断上的应用时间较短，目前对aCGH是否可以作为产前诊断工具及其潜在的不确定性存在比较大的争议。此外，对于大多数CNV的表型效应还知之甚少，因此，临床医生向患者进行解释的难度很大，导致许多临床医生拒绝以临床诊断为目的使用aCGH。根据已知的与临床疾病密切相关的一些区域定制的寡核苷酸芯片应运而生，可以降低这种不确定性，在过量信息和信息量不足之间寻求到了一个平衡。本综述的目的是从临床医生的角度探讨aCGH技术用于产前诊断所面临的问题和应用前景。

关键词：微阵列-比较基因组杂交产前基因诊断拷贝数变异遗传咨询

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Transient reliability optimization for turbine disk radial deformation

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Journal of Central South University 2016年第2期23卷 344-352页

作者：费成巍白广忱唐文忠蔡逸思高海峰 Department of Mechanical Engineering the Hong Kong Polytechnic University School of Energy and Power Engineering Beihang University School of Computer Science and Engineering Beihang University

The radial deformation design of turbine disk seriously influences the control of gas turbine high pressure turbine(HPT) blade-tip radial running clearance(BTRRC). To improve the design of BTRRC under continuous operation, the nonlinear dynamic reliability optimization of disk radial deformation was implemented based on extremum response surface method(ERSM), including ERSM-based quadratic function(QF-ERSM) and ERSM-based support vector machine of regression(SR-ERSM). The mathematical models of the two methods were established and the framework of reliability-based dynamic design optimization was developed. The numerical experiments demonstrate that the proposed optimization methods have the promising potential in reducing additional design samples and improving computational efficiency with acceptable precision, in which the SR-ERSM emerges more obviously. Through the case study, we find that disk radial deformation is reduced by about 6.5×10–5 m; δ=1.31×10–3 m is optimal for turbine disk radial deformation design and the proposed methods are verified again. The presented efforts provide an effective optimization method for the nonlinear transient design of motion structures for further research, and enrich mechanical reliability design theory.

关键词： turbine disk radial deformation reliability-based transient design optimization extremum response surface method support vector machine regression

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Thermionic emission-based interconnecting layer featuring solvent resistance for monolithic tandem solar cells with solution-processed perovskites

Thermionic emission-based interconnecting layer featuring so...

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第六届新型太阳能电池材料科学与技术学术研讨会

作者： Wallace C.H.choy Can Li Zishuai Wang Department of Electrical and Electronic Engineering The University of Hong Kong

All-perovskite tandem cell has been considered as the potential candidate for bringing the power conversion efficiency(PCE) beyond the Shockley-Queisser limit of single-junction device and yet retaining the advantages of earth-abundant materials and solution processability. However, a challenging issue of realizing such solution-processed devices is the fulfillment of complex and coupled requirements of the interconnecting layer(ICL) including solvent-resistance to protect underlying perovskite film, high electrical properties for carrier transport and recombination, and high optical transmission. In this work, a new thermionic emission-based ICL with enhanced solvent-resistance feature is demonstrated. Fundamentally, the thermionic emission plays a critical role in the electron transport process in the ICL, which is confirmed through both experimental and theoretical studies. Besides achieving high optical transmission and electrical properties, the new ICL chemically protects the underlying perovskite film by introducing a fluoride silane incorporated polyethylenimine ethoxylated(FSIP) hybrid system which also passivates the surface defects to reduce electrical loss. Our monolithic all-perovskite tandem cells demonstrate highest PCE of 17.9% [1]. Consequently, this work contributes to not only understanding the fundamental mechanism of ICL but also to promoting robust and low-cost photovoltaics.

关键词： Perovskite Tandem solar cells Interconnecting layer

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ACR MODULARIZATION AND CONSTRUCTABILITY:ACHIEVING THE CONSTRUCTION SCHEDULE

ACR MODULARIZATION AND CONSTRUCTABILITY:ACHIEVING THE CONSTR...

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第十三届国际核工程大会

作者： Rick Ricciuti Ian Love Medhat Elgohary Ed choy Len Hiebert AECL(Atomic Energy of Canada Limited)

The Advanced CANDU Reactor(ACR) Modularization and Constructability programs,built on the experience of the successful Qinshan PhaseⅢProject,are key components in achieving the cost and project sch

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“包”打天下户外摄影包导购

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数码 2008年第9期 140-145页

作者：树子 choy（摄影）

几乎每个有相机的人都离不开摄影包，而区别摄影发烧友和普通摄影爱好者的方法，有一个就是看看他有多少个摄影包。

关键词：摄影包导购户外摄影爱好者发烧友

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Preparation and Characterization of Compositionally Graded Epitaxial Barium Strontium Titanate Thin Films via Scanning Probe Microscopy

Preparation and Characterization of Compositionally Graded E...

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The 3～〔rd〕International Conference on High-Performance Ceramics(CICC-3)（2）

作者： Haydn Chen C.L.Mak C.L.choy Department of Physics and Materials Science City University of Hong Kong Hong Kong Department of Applied Physics and Materials Research Centre The Hong Kong Polytechnic University Hong Kong Department of Applied Physics and Materials Research Centre The Hong Kong Polytechnic University Hong Kong

＜正＞ Epitaxially graded barium strontium titanate （BaxSr1-x）TiO3 （x = 0.75,0.8,0.9,1.0, abbreviated as BST75, BST80, BST90 and BTO respectively） thin films were fabricated by pulsed laser deposition method on the （La0.7Sr0.3）MnO3 （LSMO）/LaAlO3 （LAO） single crystal substrate. Scanning probe microscopy with a contact mode was used to characterize the temperature dependence of polarization from room temperature to 140℃. Results indicated that the piezo-response signal of the BST graded films had an obvious change with temperature, and that the graded structures had a flatter temperature dependence of permittivity. Furthermore, the contrasts of the SPM images were lower for the ferroelectric - paraelectric （F-P） phase transition temperatures of BST 75, BST 80, and BST90, but higher for the F-P transition temperature of BTO.

关键词： Barium strontium titanate Thin film Scanning force microscopy.

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