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Understanding protein translocation across chloroplast membranes:Translocons and motor proteins

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Journal of Integrative Plant Biology 2023年第2期65卷 408-416页

作者： Da Been kim Changhee Na Inhwan Hwang Dong wook Lee Department of Integrative Food Bioscience and BiotechnologyChonnam National UniversityGwangju 61186Korea Department of Life Sciences Pohang University of Science and TechnologyPohang 37673Korea Department of Bioenergy Science and Technology Chonnam National UniversityGwangju 61186Korea Kumho Life Science Laboratory Chonnam National UniversityGwangju 61186Korea

Subcellular organelles in eukaryotes are surrounded by lipid *** an endomembrane system,vesicle trafficking is the primary mechanism for the delivery of organellar proteins to specific ***,organellar proteins for chloroplasts,mitochondria,the nucleus,and peroxisomes that are translated in the cytosol are directly imported into their target *** are a plant-specific organelle with outer and inner envelope membranes,a dual-membrane structure that is similar to *** chloroplast proteins translated by cytosolic ribosomes are thus translocated through TOC and TIC complexes(translocons in the outer and inner envelope of chloroplasts,respectively),with stromal ATPase motor proteins playing a critical role in pulling pre-proteins through these import *** the last three decades,the identity and function of TOC/TIC components and stromal motor proteins have been actively investigated,which has shed light on the action mechanisms at a molecular ***,there remains some disagreement over the exact composition of TIC complexes and genuine stromal motor *** this review,we discuss recent findings on the mechanisms by which proteins are translocated through TOC/TIC complexes and discuss future prospects for this field of research.

关键词： ATPase motor protein chloroplast molecular chaperone protein translocation TOC/TIC translocon

Metastatic liver cancer: A rare case

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World Journal of Gastroenterology 2005年第27期11卷 4281-4284页

作者： Bong-Wan kim Hee-Jung Wang In-Ho Jeong Sang-Ick Ahn Myoung-wook kim Department of Surgery

Hemangioperioltoma is a rare tumor especially when it rises in the peritoneal cavity. We present a case of a 60-year-old woman with an isolated recurrent hemangiopericytoma of the liver. The patient presented with a palpable right upper quadrant abdominal mass, which occurred 7 years after undergoing resection of a malignant hemangiopericytoma arising from the greater omentum. She had not followed up 6 mo after surgery. Various imaging studies showed a single large, well-capsulated liver tumor with central necrosis, accompanied by hypervascularity typical of a vascular tumor. Preoperative laboratory HBsAg and anti-HCV workup were both negative. Under the impression of recurrent malignant hemangiopericytoma, right trisegmentectomy was performed to completely resect the tumor. Pathological examination confirmed the diagnosis of recurrent hemangiopericytoma. Even though the incidence of the hemangiopericytoma is relatively low, malignant hemangiopericytoma has a tendency to recur frequently after a long-term disease-free interval. Also, the recurrent hemangiopericytoma is not easily detected early during follow-up until it becomes symptomatic because there are no specific tumor markers, and because of the diversity with regard to site of recurrence. The authors suggest that Positron Emission Tomogram （PET） may be a useful tool for the detection of recurrent hemangioperioltoma. We describe herein some characteristics and behaviors of malignant hemangiopericytoma, particularly after surgical resection.

关键词： Hemangiopericytoma Greater omentum Hepatic metastasis Follow-up Positron emission tomography

Simultaneous robot-assisted approach in a super-elderly patient with urothelial carcinoma and synchronous contralateral renal cell carcinoma:A case report

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World Journal of Clinical Cases 2022年第20期10卷 7153-7162页

作者： Ji Kang Yun Si Hyun kim Woong Bin kim Hee Kyung kim Sang wook Lee Department of Urology Soonchunhyang University Bucheon HospitalSoonchunhyang University College of MedicineBucheon 14584South Korea Department of Urology Soonchunhyang University Cheonan HospitalSoonchunhyang University College of MedicineCheonan 31151South Korea Department of Pathology Soonchunhyang University Bucheon HospitalSoonchunhyang University College of MedicineBucheon 14584South Korea

BACKGROUND It is rare for urothelial and renal cell carcinomas to coexist in the same patient,and even rarer for them to be detected *** of this rarity,a standard treatment has not been established and studies about overall survival are ***,physicians must modify treatments according to the individual’s situation and the stage of each *** recent years,with advances in the instruments and techniques,minimal invasive robotic surgeries have become available for advanced-stage or high-risk *** SUMMARY An 85-year-old woman with a medical history of hypertension and hyperlipidemia visited our *** had visited her local hospital complaining of intermittent,painless,gross hematuria that had started 3 mo *** computed tomography,a right renal mass and left proximal ureteral mass with hydronephrosis were found *** decided to perform robot-assisted surgery on both sides during one *** renal function and kidney loading,right partial nephrectomy was performed first,followed by left nephroureterectomy with bladder cuff *** the 6-mo follow-up,no specific symptoms were reported and dialysis was not *** were no unusual findings in the imaging study,and regular follow-up and imaging studies are *** This case report assessed the feasibility of simultaneous minimal invasive robotic surgery as an alternative to conventional open or laparoscopic surgery.

关键词： Robot surgical procedure Renal cell carcinoma Transitional cell carcinoma Minimally invasive surgical procedures Case report

Living donor liver transplantation for an adult patient with situs inversus totalis

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World Journal of Gastroenterology 2010年第18期16卷 2311-2313页

作者： Bong-Wan kim Byong-Ku Bae Weiguang Xu Hee-Jung Wang Myung-wook kim Center for Liver Transplantation and Hepatobiliary Surgery Ajou University Hospital Ajou University School of Medicine

This recipient with situs inversus totalis(SIT) was a 60-year-old female who had hepatitis B-related endstage liver *** donor evaluation showed that the right posterior section satisfied graft volume and was space-fitting in the recipient hepatic fossa when it was rotated 180 *** operation and postoperative course progressed *** weeks after living donor liver transplantation(LDLT),the graft function was disturbed by compression of bottom-placed right hepatic *** was treated with a vascular stent and subsequently the graft function was *** present case shows that LDLT for patients with SIT using a right posterior section graft is feasible.

关键词： Living donor liver transplantation Situs inversus totalis Right posterior section graft

Vehicle Positioning Based on Optical Camera Communication in V2I Environments

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Computers, Materials & Continua 2022年第8期72卷 2927-2945页

作者： Pankaj Singh Huijin Jeon Sookeun Yun Byung wook kim Sung-Yoon Jung Department of Electronic Engineering Yeungnam UniversityGyeongsan38541Korea HD Map Development Team Hyundai Autoever Co.Ltd.Seoul06171Korea Department of Information and Communication Engineering Changwon National UniversityChangwon51140Korea

Demand for precise vehicle positioning(VP)increases as autonomous vehicles have recently been drawing *** paper proposes a scheme for positioning vehicles on the move based on optical camera communication(OCC)technology in the vehicle-to-infrastructure(V2I)***-emitting diode(LED)streetlights and vehicle cameras are used as transmitters and receivers *** of streetlights are detected and traced by examining images that are obtained from cameras of ***,a scheme for analyzing visible light data extracted from the images is *** proposed vehicle positioning scheme uses information on angles between vectors that are formed under the collinearity conditions between the absolute coordinates of at least three received streetlights,and the coordinates of an image *** experiments are performed under stationary state and moving state at a speed of 5 and 20 km/*** verify the reliability of the proposed scheme,a comparison is made between the actual and estimated location of the camera in the stationary *** addition,the path of a moving vehicle and the estimated path of the vehicle are compared to check the performance of the *** performance of the proposed technique is analyzed and experimental demonstration confirms that the proposed OCC-based VP scheme achieves positioning accuracy of under 1 m.

关键词： Optical camera communication vehicle-to-infrastructure LED streetlight intelligent transport system vehicle positioning collinearity

Effect of annealing on two different niobium-clad stainless steel PEMFC bipolar plate materials

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中国有色金属学会会刊：英文版 2009年第B9期19卷 56-60页

作者： Sung-Tae HONG Dae-wook kim Yong-Joo YOU K.Scott WEIL School of Mechanical and Automotive Engineering University of Ulsan Department of Material Science and Engineering University of Ulsan Pacific Northwest National Laboratory

Niobium (Nb)-clad stainless steels(SS) produced via roll bonding are being considered for use in the bipolar plates of polymer electrolyte membrane fuel cell(PEMFC) stacks. Because the roll bonding process induces substantial work hardening in the constituent materials, thermal annealing is used to restore ductility to the clad sheet so that it can be subsequently blanked, stamped and dimpled in forming the final plate component. Two roll bonded materials, niobium clad 340L stainless steel (Nb/340L SS) and niobium clad 434 stainless steel (Nb/434 SS) were annealed under optimized conditions prescribed by the cladding manufacturer. Comparative mechanical testing conducted on each material before and after annealing shows significant improvement in ductility in both cases. However, corresponding microstructural analyses indicate an obvious difference between the two heat treated materials. During annealing, an interlayer with thick less than 1 μm forms between the constituent layers in the Nb/340L SS, whereas no interlayer is found in the annealed Nb/434 SS material. Prior work suggests that internal defects potentially can be generated in such an interlayer during metal forming operations. Thus, Nb/434 SS may be the preferred candidate material for this application.

关键词：质子交换膜燃料不锈钢材料双极板材料退火条件复合板铌聚合物电解质微观结构分析

nfluence of paradoxical sleep deprivation and sleep recovery on testosterone level in rats of different ages

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Asian Journal of Andrology 2012年第2期14卷 330-334页

作者： Mi Mi Oh Jin wook kim Myeong Heon Jin Je Jong kim Du Geon Moon Department of Urology Korea University Medical Center Seou1435-766 Korea Department of Urology Korea University Medical Center and Korea University Institute of Regenerative Medicine Seou1435-766 Korea

This study was performed to assess serum testosterone alterations induced by paradoxical sleep deprivation （PSD） and to verify their attenuation during sleep recovery （SR） based on different durations and ages. Wistar male rats aged 12 weeks for the younger group and 20 weeks for the elder group were randomly distributed into one of the following groups： a control group （cage and platform）, 3-day SD, 5-day SD, 7-day SD, 1-day SR, 3-day SR and 5-day SR groups. For PSD, the modified multiple platform method was used to specifically limit rapid eye movement （REM） sleep. Differences in the testosterone and luteinizing hormone levels between the younger group and the elder group according to duration of PSD and SR recovery were analysed. Testosterone continued to fall during the sleep deprivation period in a time-dependent manner in both the younger （P=-0.001, correlation coefficient r=-0.651） and elder groups （P=0.001, correlation coefficient r=-0.840）. The elder group showed a significantly lower level of testosterone compared with the younger group after PSD. Upon SR after 3 days of PSD, the testosterone level continued to rise for 5 days after sleep recovery in the younger group （P=0.013）, whereas testosterone concentrations failed to recover until day 5 in the elder group. PSD caused a more detrimental effect on serum testosterone in the elder group compared to the younger group with respect to decreases in luteinizing hormone （LH） levels. The replenishment of serum testosterone level was prohibited in the elder group suggesting that the effects of SD/SR may be age-dependent. The mechanism by which SD affects serum testosterone and how age may modify the process are still unclear.

关键词： luteinizing hormone paradoxical sleep deprivation recovery testosterone

Recent advances in stem cell therapy for neurodegenerative disease: Three dimensional tracing and its emerging use

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World Journal of Stem Cells 2021年第9期13卷 1215-1230页

作者： Il-Kwon kim Jun-Hee Park Bomi kim Ki-Chul Hwang Byeong-wook Song Institute for Bio-Medical Convergence Catholic Kwandong University International St.Mary’s HospitalIncheon Metropolitan City 22711South Korea Institute for Bio-Medical Convergence College of MedicineCatholic Kwandong UniversityGangwon-do 25601South Korea

Neurodegenerative disease is a brain disorder caused by the loss of structure andfunction of neurons that lowers the quality of human life. Apart from the limitedpotential for endogenous regeneration, stem cell-based therapies hold considerablepromise for maintaining homeostatic tissue regeneration and enhancingplasticity. Despite many studies, there remains insufficient evidence for stem celltracing and its correlation with endogenous neural cells in brain tissue with threedimensionalstructures. Recent advancements in tissue optical clearing techniqueshave been developed to overcome the existing shortcomings of cross-sectionaltissue analysis in thick and complex tissues. This review focuses on recentprogress of stem cell treatments to improve neurodegenerative disease, andintroduces tissue optical clearing techniques that can implement a threedimensionalimage as a proof of concept. This review provides a more comprehensiveunderstanding of stem cell tracing that will play an important role inevaluating therapeutic efficacy and cellular interrelationship for regeneration inneurodegenerative diseases.

关键词： Cell tracing Neurodegenerative disease Stem cells Three-dimensional imaging Tissue clearing

Arthroscopic treatment of iliopsoas tendinitis after total hip arthroplasty with acetabular cup malposition: Two case reports

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World Journal of Clinical Cases 2020年第21期8卷 5326-5333页

作者： Heejae Won kwang-Hwan kim Jae-wook Jung Shin-Yoon kim Seung-Hoon Baek Department of Orthopedic Surgery School of MedicineKyungpook National UniversityKyungpook National University Hospital130 Dongdeok-roJung-GuDaegu 41944South Korea

BACKGROUND Optimal treatment for iliopsoas tendinitis after total hip arthroplasty(THA)with cup malposition,iliopsoas release alone or with cup revision,is controversial,particularly in young,active ***,arthroscopic iliopsoas tendon(IPT)release in these patients has been rarely described,and midterm effects of this procedure on THA longevity and groin pain recurrence remain *** performed arthroscopic IPT release after THA and report midterm outcomes in two young patients with acetabular cup *** SUMMARY In the two patients,groin pain started early after *** examination revealed nonspecific findings,and laboratory tests showed no evidence of *** and computed tomography showed reduced acetabular component anteversion angle and anterior cup prominence of more than 16 *** therapeutic diagnosis,ultrasonography-guided lidocaine with steroid was injected into the IPT *** both patients,groin pain improved initially but worsened after a few ***,the patients underwent arthroscopic IPT release under spinal *** revealed synovitis with fibrous tissues around the IPT and various lesions related to the implants after *** tenotomy and debridement with biopsy were performed;histopathologic studies showed chronic inflammation with synovial *** patients were encouraged to start walking immediately after surgery,and they returned to complete daily function early after *** experienced no recurrence of groin pain or any implant-related problems 5 years *** Arthroscopic IPT release for cup malposition produced excellent midterm outcomes without recurrence of groin pain and implant-related problems.

关键词： Iliopsoas tendinitis Total hip arthroplasty Cup malposition Arthroscopy Tenotomy Case report