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Recombination and repeat-induced point mutation landscapes reveal trade-offs between the sexual and asexual cycles of Magnaporthe oryzae

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Journal of Genetics and Genomics 2024年第7期51卷 723-734页

作者： Xifang Zong Yaxin Lou Mengshuang Xia kunyang zhao Jingxuan Chen Ju Huang Sihai Yang Long Wang State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjingJiangsu 210000China State Key Laboratory for Crop Genetics and Germplasm Enhancement Bioinformatics CenterAcademy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjingJiangsu 210000China Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjingJiangsu 210000China

The fungal disease caused by Magnaporthe oryzae is one of the most devastating diseases that endanger many crops *** shows that sexual reproduction can be advantageous for fungal diseases as hybridization facilitates ***,the pervasive clonal lineages of *** observed in natural fields contradict this expectation.A better understanding of the roles of recombination and the fungi-specific repeat-induced point mutation(RIP)in shaping its evolutionary trajectory is essential to bridge this knowledge *** we systematically investigate the RIP and recombination landscapes in *** using a whole genome sequencing data from 252 population samples and 92 cross *** data reveal that the RIP can robustly capture the population history of ***,and we provide accurate estimations of the recombination and RIP rates across different *** ***,our results highlight a parent-of-origin bias in both recombination and RIP rates,tightly associating with their sexual potential and variations of effector *** bias suggests a critical trade-off between generating novel allelic combinations in the sexual cycle to facilitate host-jumping and stimulating transposon-associated diversification of effectors in the asexual cycle to facilitate host *** findings provide unique insights into understanding the evolution of blast fungus.

关键词： Magnaporthe oryzae Crossover Repeat-induced point mutation(RiP) Effectors Sexual reproduction

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Experimental study on the evolution of bright-dark pulses in a 2 μm mode-locked thulium/holmium co-doped fiber laser

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Optoelectronics Letters 2024年

作者： Wang Xiaofa Wang Jie zhao kunyang Chongqing Key Laboratory of Photoelectronic Information Sensing and Transmitting Technology School of Optoelectronic Engineering Chongqing University of Posts and Telecommunications

We experimentally demonstrate the effects of pump power and polarization state on the evolution of bright-dark pulses (BDPs) in a figure-eight passively mode-locked thulium/holmium co-doped fiber laser (THDFL) using nonlinear amplifying loop mirror (NALM) technology. As the pump power increases from 0.5 W to 1 W， the BDPs are separated and exhibit a linear increase in the time interval between the bright pulse (BP) and dark pulse (DP)， which roughly corresponds to the reciprocal of the modulation frequency in the radio frequency (RF) spectrum. Additionally， the polarization state in the cavity is altered by adjusting the polarization controller (PC)， the central wavelength of spectrum， pulse waveform， and full width at half maximum (FWHM) of BDPs in different states are presented.

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西南喀斯特地区农村人居环境整治的特殊困难及对策研究——基于贵州省的个案调查

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中国西部 2022年第3期 80-89页

作者：徐彦胜赵昆炀张鸣鸣农业农村部沼气科学研究所成都610041

改善农村人居环境是实施乡村振兴战略的一项重要任务,随着农村人居环境整治三年行动任务的完成,各地农村人居环境得到明显改善。但我国西南喀斯特地区由于特殊的地质条件和脆弱的生态环境,农村人居环境整治面临一些亟待解决的特殊困难... 详细信息

改善农村人居环境是实施乡村振兴战略的一项重要任务,随着农村人居环境整治三年行动任务的完成,各地农村人居环境得到明显改善。但我国西南喀斯特地区由于特殊的地质条件和脆弱的生态环境,农村人居环境整治面临一些亟待解决的特殊困难。文章以贵州省为例,从我国西南喀斯特地区农村人居环境整治现状出发,分析了该类地区农村人居环境整治存在的问题,提出了出台针对性整治策略及支持保障方案、构建多元投入格局、精细化系统化推进农村人居环境整治提升等对策建议。

关键词：喀斯特地区乡村振兴乡村治理生态建设农村人居环境

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欧盟数据保护官制度及对中国的启示——以欧盟《一般数据保护条例》为研究对象

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网络信息法学研究 2020年第2期 -页

作者：赵鲲洋烟台大学法学院

欧盟《一般数据保护条例》于2018年5月25日在欧盟全体成员国正式生效。该条例中的数据保护官制度为全球之前沿实践,值得我国归纳、反思并借鉴。欧盟数据保护官制度主要包括三个方面内容:数据保护官的设立、地位及职责。数据保护官的设... 详细信息

欧盟《一般数据保护条例》于2018年5月25日在欧盟全体成员国正式生效。该条例中的数据保护官制度为全球之前沿实践,值得我国归纳、反思并借鉴。欧盟数据保护官制度主要包括三个方面内容:数据保护官的设立、地位及职责。数据保护官的设立采用强制设立与自愿设立相结合的原则,其是具有独立性的特殊职位,并旨在保证条例在数据控制和处理主体内部有效实施,维护数据主体的合法权益。数据保护官的独立性不仅是其履职的关键,更是条例有序推进的关键。我国未来的数据保护立法应从现有的普遍强制设立原则转向强制与自愿相结合的原则,明确数据保护官以维护数据主体合法权益为主要职责,并进一步强化数据保护官的独立性要求。

关键词：个人数据保护《一般数据保护条例》数据保护官

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Global Optimization‑Based Energy Management Strategy for Series–Parallel Hybrid Electric Vehicles Using Multi‑objective Optimization Algorithm

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Automotive Innovation 2023年第3期6卷 492-507页

作者： Kegang zhao kunyang He Zhihao Liang Maoyu Mai School of Mechanical and Automotive Engineering South China University of TechnologyGuangzhou510610China

The study of series–parallel plug-in hybrid electric vehicles(PHEVs)has become a research hotspot in new energy *** global optimal Pareto solutions of energy management strategy(EMS)play a crucial role in the development of *** paper presents a multi-objective global optimization algorithm for the EMS of *** algorithm combines the Radau Pseudospectral Knotting Method(RPKM)and the Nondominated Sorting Genetic Algorithm(NSGA)-II to optimize both energy conservation and battery lifespan under the suburban driving conditions of the New European Driving *** driving conditions are divided into stages at evident mode switching points and the optimal objectives are computed using *** RPKM results serve as the fitness values in iteration through the NSGA-II *** results of the algorithm applied to a PHEV simulation show a 26.74%–53.87%improvement in both objectives after 20 iterations compared to the solutions obtained using only *** proposed algorithm is evaluated against the weighting dynamic programming and is found to be close to the global optimality,with the added benefits of faster and more uniform solutions.

关键词： Plug-in hybrid electric vehicles Energy management strategy Multi-objective optimization Global optimization NSGA-II Radau pseudospectral knotting method

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Design of a Flexible Bionic Ankle Prosthesis Based on Subject-specific Modeling of the Human Musculoskeletal System

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Journal of Bionic Engineering 2023年第3期20卷 1008-1020页

作者： Jianqiao Jin kunyang Wang Lei Ren Zhihui Qian Wei Liang Xiaohan Xu Shun zhao Xuewei Lu Di zhao Xu Wang Luquan Ren Key Laboratory of Bionic Engineering Ministry of EducationJilin UniversityChangchun130025China Weihai Institute for Bionics Jilin UniversityWeihai264402China School of Mechanical Aerospace and Civil EngineeringUniversity of ManchesterManchesterM139PLUK

A variety of prosthetic ankles have been successfully developed to reproduce the locomotor ability for lower limb amputees in daily lives. However, they have not been shown to sufficiently improve the natural gait mechanics commonly observed in comparison to the able-bodied, perhaps due to over-simplified designs of functional musculoskeletal structures in prostheses. In this study, a flexible bionic ankle prosthesis with joints covered by soft material inclusions is developed on the basis of the human musculoskeletal system. First, the healthy side ankle–foot bones of a below-knee amputee were reconstructed by CT imaging. Three types of polyurethane rubber material configurations were then designed to mimic the soft tissues around the human ankle, providing stability and flexibility. Finite element simulations were conducted to determine the proper design of the rubber materials, evaluate the ankle stiffness under different external conditions, and calculate the rotation axes of the ankle during walking. The results showed that the bionic ankle had variable stiffness properties and could adapt to various road surfaces. It also had rotation axes similar to that of the human ankle, thus restoring the function of the talocrural and subtalar joints. The inclination and deviation angles of the talocrural axis, 86.2° and 75.1°, respectively, as well as the angles of the subtalar axis, 40.1° and 29.9°, were consistent with the literature. Finally, dynamic characteristics were investigated by gait measurements on the same subject, and the flexible bionic ankle prosthesis demonstrated natural gait mechanics during walking in terms of ankle angles and moments.

关键词： Ankle prosthesis Bionic design Soft materials Variable stiffness Gait mechanics

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Fiber-dominated Soft Actuators Inspired by Plant Cell Walls and Skeletal Muscles

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Journal of Bionic Engineering 2023年第3期20卷 982-991页

作者： Luquan Ren Qian Wu Qingping Liu Lei Ren kunyang Wang Xueli Zhou Zhenguo Wang Yulin He Che zhao Bingqian Li Key Laboratory of Bionic Engineering Ministry of EducationJilin UniversityChangchun130022China School of Mechanical Aerospace and Civil EngineeringUniversity of ManchesterManchesterM139PLUK School of Aerospace and Mechanical Engineering Changzhou Institute of TechnologyChangzhou213032China

Morphing botanical tissues and animal muscles are all fiber-mediated composites, in which fibers play a passive and active role, respectively. Herein, inspired by the mechanism of fibers functioning in morphing botanical tissues and animal muscles, we propose two sorts of fiber-dominated composite actuators. First, inspired by the deformation of awned seeds in response to humidity change, we fabricate passive fiber-dominated actuators using non-active aligned carbon fibers via 4D printing method. The effects of process parameters, structural parameters, and fiber angles on the deformation of the printed actuators are examined. The experimental results show that the orientation degree is enhanced, resulting in a better swelling effect as the printing speed increases. Then, motivated by the actuation mechanism of skeletal muscle, we prepare active fiber-dominated actuators using active polyurethane fibers via 4D printing and pre-stretching method. The effect of fiber angle and loading on the actuation mode is experimentally analyzed. The experimental results show that the rotation angle of the actuator gradually decreases with the angle from 45° to 60°. When the fiber angle is 0° and 90°, the driver basically stops rotating while shrinking along the loading direction. Based on the above actuation mechanisms, identical contraction behaviors are realized both in passive and active fiber-dominated soft actuators. This work provides a validation method for biologically actuation mechanisms via 4D printing technique and smart materials and adds further insights to the design of bioinspired soft actuators.

关键词： Bioinspired Biological materials Fiber-mediated composites Actuator 4D printing

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Mach Number Prediction for a Wind Tunnel Based on the CNN-LSTM-Attention Method

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Instrumentation 2023年第4期10卷 64-82页

作者： zhao Luping WU kunyang College of Information Science and Engineering Northeastern UniversityShenyang 11081China

The test section’s Mach number in wind tunnel testing is a significant metric for evaluating system *** quality of the flow field in the wind tunnel is contingent upon the system's capacity to maintain stability across various working *** process flow in wind tunnel testing is inherently complex,resulting in a system characterized by nonlinearity,time lag,and multiple working *** implement the predictive control algorithm,a precise Mach number prediction model must be ***,this report studies the method for Mach number prediction modelling in wind tunnel flow fields with various working ***,this paper introduces a continuous transonic wind *** key physical quantities affecting the flow field of the wind tunnel are determined by analyzing its structure and blowing ***,considering the nonlinear and time-lag characteristics of the wind tunnel system,a CNN-LSTM model is employed to establish the Mach number prediction model by combining the 1D-CNN algorithm with the LSTM model,which has long and short-term memory ***,the attention mechanism is incorporated into the CNN-LSTM prediction model to enable the model to focus more on data with greater information importance,thereby enhancing the model's training *** application results ultimately demonstrate the efficacy of the proposed approach.

关键词： Wind Tunnel Test,Mach Number Prediction CNN-LSTM Attention Mechanism

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结构优化在空间可展开天线中的应用及展望

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电子机械工程 2024年第3期40卷 1-8页

作者：杨军刚马小飞姚培杨钊谢卓林坤阳高彤孟亮西安空间无线电技术研究所陕西西安710199 西北工业大学航宇材料结构一体化设计与增材制造装备技术国际联合研究中心陕西西安710072

空间可展开天线是航天领域发展的重要方向,具有重大战略意义和重要经济社会价值。首先,对结构优化技术、面向增材制造的拓扑优化与点阵填充等先进轻量化设计技术的应用进行综述;然后,根据典型使用环境和设计要求,着重介绍近年来结构优... 详细信息

空间可展开天线是航天领域发展的重要方向,具有重大战略意义和重要经济社会价值。首先,对结构优化技术、面向增材制造的拓扑优化与点阵填充等先进轻量化设计技术的应用进行综述;然后,根据典型使用环境和设计要求,着重介绍近年来结构优化技术在空间可展开天线零部件结构优化和馈源阵支撑结构概念设计中的典型应用案例;最后,考虑未来空间超大口径及高精度可展开天线的设计需求,对先进轻量化设计技术的应用前景进行展望。结构优化技术与设计经验相结合,正在推动设计理念的创新,将成为先进航天器设计的核心工具和必备的标准流程。

关键词：空间可展开天线轻量化设计结构优化

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脾多肽对急性肺损伤大鼠TLR9信号相关因子的影响

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贵州医科大学学报 2017年第3期42卷 296-299页

作者：苏俊孟郭晖夏飞申梓玄马华星赵坤垟贵州医科大学贵州贵阳550000 贵州医科大学附院急诊科贵州贵阳550004

目的:分析脾多肽对急性肺损伤(ALI)大鼠Toll样受体9(TLR9)信号相关因子的影响。方法:60只无特定病原体级(SPF)Wistar大鼠,40只采用BIM-Ⅱ型水平式生物撞击机持续撞击右侧胸部复制ALI大鼠模型,造模成功后的大鼠均分为脾多肽组(n=20,经耳... 详细信息

目的:分析脾多肽对急性肺损伤(ALI)大鼠Toll样受体9(TLR9)信号相关因子的影响。方法:60只无特定病原体级(SPF)Wistar大鼠,40只采用BIM-Ⅱ型水平式生物撞击机持续撞击右侧胸部复制ALI大鼠模型,造模成功后的大鼠均分为脾多肽组(n=20,经耳静脉注射5mg/Kg脾多肽)及ALI组(n=20,经耳静脉注射等量生理盐水);20只作为对照组(经耳静脉注射等量生理盐水),注射6 h后处死大鼠,HE染色观察3组大鼠肺组织病理学改变,ELISA法检测大鼠灌洗液中肿瘤坏死因子(TNF-α)、白细胞介素-8(IL-8)和白细胞介素-6(IL-6)的水平,RT-PCR法检测肺组织中NF-κB、髓样分化因子(Myd88)、Toll样受体(TLR9)mRNA的水平。结果:HE染色显示,ALI与脾多肽组均出现了ALI表现;与对照组比较,ALI组和脾多肽组大鼠TNF-α、IL-8及IL-6蛋白表达水平,NF-κB、Myd88及TLR9 mRNA的表达均增加,脾多肽组低于ALI组,差异有统计学意义(P<0.05)。结论:脾多肽可显著下调ALI大鼠TLR9信号相关因子表达水平,降低ALI程度。

关键词：脾多肽急性肺损伤 Toll样受体9 创伤髓样分化因子

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