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A peptide chain release factor 2a gene regulates maize kernel development by modulating mitochondrial function

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The Crop Journal 2023年第6期11卷 1731-1741页

作者： Hui Zhang Yijian Feng kunyang song Guofang Li Jiao Jin Jingjing Gao Yongtian Qin Hongqiu Wang Jinpeng Cheng Zonghua Liu Jihua Tang Zhiyuan Fu Key Laboratory of Wheat and Maize Crops Science/Collaborative Innovation Center of Henan Grain Crops/College of Agronomy Henan Agricultural UniversityZhengzhou 450046HenanChina The Shennong Laboratory Zhengzhou 450002HenanChina Hebi Academy of Agricultural Sciences Hebi 458030HenanChina Yunnan Tang Jihua Expert Workstation/Zhaotong Academy of Agricultural Sciences Zhaotong 657000YunnanChina

Mitochondrial protein translation that is essential for aerobic energy production includes four essential steps of the mitochondrial ribosome cycle,namely,initiation,elongation,termination of the polypeptide,and ribosome *** termination initiates when a stop codon enters the A site of the mitochondrial ribosome where it is recognized by a dedicated peptide release factor(RF).However,RFs and mechanisms involved in translation in plant mitochondria,especially in monocotyledons,remain largely ***,we identified a crumpled kernel(crk5 allele)mutant,with significantly decreased kernel size,100-kernel weight,and an embryo-lethal *** Crk5 allele was isolated using map-based cloning and found to encode a mitochondrial localization *** it is an ortholog of Arabidopsis mitochondrial RF2a,we named the gene ***2a is missing the 5th–7th exons in the crk5 resulting in deletion of domains containing motifs GGQ and SPF that are essential for release activity of RF,mitochondrial ribosome binding,and stop codon *** blot and qRT-PCR analyses indicate that the crk5 mutation results in abnormal mitochondrion structure and ***,we observed a feedback loop in the crk5 with up-regulated transcript levels detected for several mitochondrial ribosome and mitochondrial-related components,in particular mitochondrial complexes CI,CIV,and a ribosome assembly related ***,our data support a crucial role for ZmmtRF2a in regulation of mitochondrial structure and function in maize.

关键词： Embryo lethality Mitochondrion Peptide release factor 2 Zea mays

植物活性物质调控反刍动物甲烷排放作用机理研究进展

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东北农业科学 2018年第4期43卷 36-40页

作者：宋坤烊闫晓刚吉林省农业科学院畜牧科学分院吉林公主岭136100

植物活性物质作为饲料添加剂,具有天然无害、抑制细菌等作用,被认为是一种新型的甲烷抑制剂。本文综述了植物活性物质作为反刍动物甲烷抑制剂的作用效果、作用机理和存在的问题,旨在为植物活性物质作为甲烷抑制剂在生产中的应用提供参考。

关键词：植物活性物质反刍动物甲烷作用机理

一种用于射频前端的谐波整形低功耗N通道滤波器

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无线电工程 2024年第7期54卷 1795-1802页

作者：宋堃阳蒋品群宋树祥蔡超波岑明灿广西类脑计算与智能芯片重点实验室广西桂林541004 广西高校集成电路与微系统重点实验室广西桂林541004 广西师范大学电子与信息工程学院/集成电路学院广西桂林541004

针对传统射频前端N通道滤波器功耗高,不能根据应用要求选择通过或抑制信号的高次谐波等问题,设计了一款谐波整形低功耗N通道滤波器。该滤波器通过添加具有适当权重的额外路径,可实现谐波抑制和整形的功能。同时,将本振Local Oscillator(... 详细信息

针对传统射频前端N通道滤波器功耗高,不能根据应用要求选择通过或抑制信号的高次谐波等问题,设计了一款谐波整形低功耗N通道滤波器。该滤波器通过添加具有适当权重的额外路径,可实现谐波抑制和整形的功能。同时,将本振Local Oscillator(LO)信号的n次谐波信号作为时钟信号,降低LO信号频率,时钟信号发生器的动态功耗降低到原来的1/n。SMIC 180 nm CMOS工艺的后仿真结果表明,10通道带通滤波器的中心频率在0.1~6.0 GHz可调,增益为0~5 dB,噪声系数小于4.6 dB,功耗为3.5~6.0 mW,输入三阶交调点(Input 3rd-order Intermodulation Point,IIP3)大于10 dBm,3次和5次谐波抑制分别大于65、90 dB,表明该滤波器具有谐波抑制和谐波整形的功能,且具有较低的功耗。

关键词： N通道滤波器带通滤波器谐波整形谐波抑制低功耗

生长期肉鸡玉米和玉米干酒糟及其可溶物的代谢能和净能评定

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动物营养学报 2019年第2期31卷 792-800页

作者：班志彬杨华明于维宋坤烊张莹李立佳郎朝丽梁浩苏斯瑶闫晓刚吉林省农业科学院畜牧科学分院长春130000 延边大学农学院延吉133002

本研究采用禽用开放式呼吸测热装置进行能量代谢试验,共2个试验,分别测定玉米和玉米干酒糟及其可溶物(DDGS)的代谢能和净能。试验1和试验2各选用25～28日龄ROSS308雄鸡180只,采用近似拉丁方设计,配制2种基础饲粮和4种待测饲粮,每种饲粮... 详细信息

本研究采用禽用开放式呼吸测热装置进行能量代谢试验,共2个试验,分别测定玉米和玉米干酒糟及其可溶物(DDGS)的代谢能和净能。试验1和试验2各选用25～28日龄ROSS308雄鸡180只,采用近似拉丁方设计,配制2种基础饲粮和4种待测饲粮,每种饲粮6个重复,每个重复10只鸡。试验1中待测玉米(玉米吉粳511、吉农823)以40%比例替代玉米-豆粕型基础饲粮。试验2中待测玉米DDGS(中粮玉米DDGS、吉燃玉米DDGS)以30%比例替代玉米-豆粕型基础饲粮中能量部分。试验1和试验2各分3期,每期31 d,每期试验中使用雄性鸡雏60只,随机分为3组,每组20只,试验鸡第1～21天在鸡舍内分组饲养,第21天称重后从每组中选择4只试验鸡,分别放入呼吸测热装置的代谢室内,测定气体交换和排泄物总量,适应3d、呼吸测热3 d、绝食测热3 d,呼吸测热的同时进行消化代谢试验。结果表明:1)试验1中,玉米待测饲粮的表观代谢能、代谢能摄入量、沉积能及净能显著高于基础饲粮(P0.05)。2)试验2中,玉米DDGS待测饲粮的平均日采食量、代谢能摄入量及产热量显著高于基础饲粮(P0.05)。干物质基础的玉米吉粳511、吉农823对于ROSS 308雄鸡(25～28日龄)的表观代谢能分别为13.79和13.64 MJ/kg,净能分别为10.77和10.57 MJ/kg。干物质基础的中粮玉米DDGS、吉燃DDGS对于ROSS 308雄鸡(25～28日龄)表观代谢能分别为10.37和10.74 MJ/kg,净能分别为6.43和6.57 MJ/kg。

关键词：肉鸡净能玉米玉米DDGS

A novel tomato SUMO E_3 ligase,SlSIZ_1,confers drought tolerance in transgenic tobacco

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Journal of Integrative Plant Biology 2017年第2期59卷 102-117页

作者： song Zhang kunyang Zhuang Shiju Wang Jinlian Lv Na'na Ma Qingwei Meng State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai'an 271018China

SUMOylation is an impo^ant post-translational modification process that regulates different cellular functions in eukaryotes. SIZ/PIAS-type SAP and Mizl （SIZ0 proteins exhibit SUMO E3 ligase activity, which modulates SUMOylation. However, SIZI in tomato has been rarely investigated. In this study, a tomato SIZI gene （SISIZI） was isolated and its molecular characteristics and role in tolerance to drought stress are described. SISIZI was upregulated by cold, sodium chloride （NaCI）, polyethylene glycol （PEG）, hydrogen peroxide （H20~） and abscisic acid （ABA）, and the corresponding proteins were localized in the nucleus. The expression of SISIZI in Arabidopsis thaliana （Arabidopsis） sizl-2 mutants partially complemented the phenotypes of dwarf, cold sensitivity and ABA hypersensitivity. SISIZI also exhibited the activity of SUMO E3 ligase to promote the accumulation of SUMO conjugates. Under drought stress, the ectopic expression of SISIZl in transgenic tobacco lines enhanced seed germination and reduced the accumulation of reactive oxygen species..SISIZl overexpression conferred the plants with improved growth, high free proline content, minimal malondialdehyde accumulation and increased accumulation of SUMO conjugates. SISIZ1 is a functional homolog of Arabidopsis SIZ1 with SUMO E3 ligase activity. Therefore, overexpression of SISIZ1 enhanced the tolerance of transgenic tobacco to drought stress.

关键词： drought stress SUMO E3 ligase SlSIZ1 SUMO conjugates tomato transgenic tobacco

Intrinsic Kinematics of the Tibiotalar and Subtalar Joints during Human Walking based on Dynamic Biplanar Fluoroscopy

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Journal of Bionic Engineering 2023年第5期20卷 2059-2068页

作者： Shengli Wang Zhihui Qian Xiangyu Liu Guangsheng song kunyang Wang Jianan Wu Jing Liu Lei Ren Luquan Ren Key Laboratory of Bionic Engineering Jilin UniversityChangchun130022China School of Mechanical Aerospace and Civil EngineeringUniversity of ManchesterManchesterM139PLUK

Accurate knowledge of the kinematics of the in vivo Ankle Joint Complex(AJC)is critical for understanding the biomechanical function of the foot and assessing postoperative rehabilitation of ankle disorders,as well as an essential guide to the design of ankle–foot assistant ***,detailed analysis of the continuous 3D motion of the tibiotalar and subtalar joints during normal walking throughout the stance phase is still considered to be *** this study,dynamic radiographs of the hindfoot were acquired from eight subjects during normal *** motions with six Degrees of Freedom(DOF)and the coupled patterns of the two joints were *** was found that the movements of the two joints were mostly in opposite directions(including rotation and translation),mainly in the early and late *** were significant differences in the Range of Motion(ROM)in Dorsiflexion/Plantarflexion(D/P),Inversion/Eversion(In/Ev),and Anterior–Posterior(AP)and Medial–Lateral(ML)translation of the tibiotalar and subtalar joints(p<0.05).Plantarflexion of the tibiotalar joint was coupled with eversion and posterior translation of the subtalar joint during the impact phase(R^(2)=0.87 and 0.86,respectively),and plantarflexion of the tibiotalar joint was coupled with inversion and anterior translation of the subtalar joint during the push-off phase(R^(2)=0.93 and 0.75,respectively).This coordinated coupled motion of the two joints may be a manifestation of the AJC to move flexibly while bearing weight and still have stability.

关键词： Dynamic biplane radiography Tibiotalar joint Subtalar joint In vivo kinematics Coupled motion Bionic design

Design,Testing and Control of a Magnetorheological Damper for Knee Prostheses

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Journal of Bionic Engineering 2024年第4期21卷 1788-1800页

作者： Hounan song Yu Cao Wei Chen Lei Ren Yongxin Ma kunyang Wang Xu Wang Yao Zhang Luquan Ren Key Laboratory of Bionic Engineering Ministry of EducationJilin UniversityChangchun130022People’s Republic of China Department of Mechanical Aerospace and Civil EngineeringThe University of ManchesterManchesterM139PLUK

This study aims to develop a magnetorheological(MR)damper for semi-active knee prostheses to restore the walking ability of transfemoral *** core dimensions of the MR damper were determined via theoretical magnetic field calculations,and the theoretical relationship between current and joint torque was derived through electromagnetic ***,a physical prototype of the semi-active prosthetic knee equipped with the MR damper was *** on the data obtained from angle sensor,pressure sensor(loadcell),and inertial measurement unit(IMU)on the prosthesis,a matching control algorithm is *** joint torque of the MR damper can be adaptively adjusted according to the walking speed of the amputee,allowing the amputee to realize a natural *** effectiveness of the control program was verified by the ADAMS and MATLAB *** results of the test and simulation show that the MR damper can provide sufficient torque needed for normal human activities.

关键词： Semi-active knee prosthesis Magnetorheological damper Theoretical analysis ADAMS and MATLAB co-simulation

CNTNAP2 intracellular domain(CICD)generated byγ-secretase cleavage improves autism-related behaviors

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Signal Transduction and Targeted Therapy 2023年第7期8卷 3415-3426页

作者： Jing Zhang Fang Cai Renbin Lu Xiaoliang Xing Lu Xu kunyang Wu Zishan Gong Qing Zhang Yun Zhang Mengen Xing Weihong song Jia-Da Li Furong Laboratory Center for Medical GeneticsHunan Key Laboratory of Animal Models for Human DiseasesHunan Key Laboratory of Medical GeneticsHunan International Scientific and Technological Cooperation Base of Animal Models for Human DiseasesSchool of Life SciencesCentral South UniversityChangsha 410078 HunanChina Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)WenzhouZhejiang 325000China Townsend Family Laboratories Department of PsychiatryThe University of British Columbia2255 Wesbrook MallVancouverBC V6T 1Z3Canada Institute of Aging Key Laboratory of Alzheimer’s Disease of Zhejiang ProvinceSchool of Mental Health and Kangning HospitalThe Second Affiliated Hospital and Yuying Children’s HospitalWenzhou Medical UniversityWenzhouZhejiang 325035China Advanced Innovation Center for Human Brain Protection The National Clinical Research Center for Geriatric DiseaseXuanwu HospitalCapital Medical UniversityBeijing 100053China

As the most prevalent neurodevelopmental disorders in children,autism spectrum disorders(ASD)are characterized by deficits in language development,social interaction,and repetitive behaviors or inflexible *** associated protein like 2(CNTNAP2),encoding a single transmembrane protein(CNTNAP2)with 1331 amino acid residues,is a widely validated ASD-susceptible gene.

关键词： behaviors cleavage residues

Occurrence, variations, and risk assessment of neonicotinoid insecticides in Harbin section of the songhua River, northeast China

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Environmental Science and Ecotechnology 2021年第4期8卷 85-93页

作者： Zhikun Liu song Cui Leiming Zhang Zulin Zhang Rupert Hough Qiang Fu Yi-Fan Li Lihui An Mingzhi Huang kunyang Li Yuxin Ke Fuxiang Zhang International Joint Research Center for Persistent Toxic Substances(IJRC-PTS) School of Water Conservancy and Civil EngineeringNortheast Agricultural UniversityHarbinHeilongjiang150030China Research Center for Eco-Environment Protection of songhua River Basin Northeast Agricultural UniversityHarbinHeilongjiang 150030China Air Quality Research Division Science and Technology BranchEnvironment and Climate Change CanadaTorontoOntarioM3H 5T4Canada The James Hutton Institute CraigiebucklerAberdeenAB158QHUK State Environmental Protection Key Laboratory of Estuarine and Coastal Research Chinese Research Academy of Environmental SciencesBeijing100012China School of Environment South China Normal UniversityGuangzhou510006China

Neonicotinoid insecticides(NNIs)have been intensively used and exploited,resulting in their presence and accumulation in multiple environmental *** herein investigated the current levels of eight major NNIs in the Harbin section of the songhua River in northeast China,providing the first systematic report on NNIs in this *** least four NNIs in water and three in sediment were detected,with total concentrations ranging from 30.8 to 135 ng L^(-1) and from 0.61 to 14.7 ng g^(-1) dw,*** spatial variations in surface water NNIs concentrations were observed in tributary than mainstream(psonghua River were determined using the species sensitivity distribution(SSD)approach,resulting in a value of 355 ng L^(-1) for acute hazardous concentration for 5%of species(HC5)and 165 ng L^(-1) for chronic *** organisms at low trophic levels were more vulnerable to potential harm from NNIs.

关键词： Neonicotinoid insecticides Sediment-water exchange Human exposure Species sensitivity distribution