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Comparative Analysis of Transcriptomes among Bombyx mori Strains and Sexes Reveals the Genes Regulating Melanic Morph and the Related Phenotypes

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Comparative Analysis of Transcriptomes among Bombyx mori Str...

中国蚕学会第九届青年学术研讨会

作者： Songzhen He Xiaoling Tong kunpeng lu Yaru lu Jiangwen luo Wenhao Yang Min Chen Min-jin Han Hai Hu Cheng lu Fangyin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural MinistrySouthwest University

As a source of insect polymorphism,melanism plays an important role in ecological adaption and usually endows advantageous phenotypic-effects on ***,due to the mechanistic diversity,there are knowledge gaps in the molecular mechanisms underlying melanism and the related *** silk moths,a recessive melanic mutant（sex-controlled melanism,sml） strain exhibits extended adult *** took a transcriptome approach to perform a comparative analysis between this sml strain and a wild-type strain（Dazao）.Our analysis resulted in the identification of 59 unique differentially expressed genes in the melanic *** key genes（laccase2 and yellow） involved in melanin formation were significantly up-regulated in melanic *** laccase2 B-type isoform（BGIBMGA006746） was found to likely participate in the silkworm cuticular melanism process at late pupal ***,we discovered 22 cuticular protein encoding genes with the possible function in melanin transport and/or *** on our findings,we presume that the longer survival of the melanic sml male moths might be associated with the enhanced antioxidant defense systems and a reduction in the insulin/IGF-1 signaling pathway（IIS）.These findings will facilitate the understanding of the molecular basis underlying melanism and the derived phenotypic-effects.

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Comparative analysis of the integument transcriptomes of the black dilute mutant and the wild type silkworm Bombyx mori

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Comparative analysis of the integument transcriptomes of the...

中国蚕学会第九届青年学术研讨会

作者： Songyuan Wu Xiaoling Tong Chenxing Peng Gao Xiong kunpeng lu Hai hu Duan Tan Chunlin Li Minjin Han Cheng lu Fangyin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural MinistrySouthwest University

The insect cuticle is a critical protective shell that is composed predominantly of chitin and various cuticular proteins and ***,insects often change their surface pigment patterns in response to selective pressures,such as threats from predators,sexual selection and environmental ***,the molecular mechanisms underlying the construction of the epidermis and its pigmentation patterns are not fully *** Lepidoptera,the silkworm is a favorable model for color pattern *** black dilute（bd） mutant of silkworm is the result of a spontaneous mutation;the larval body color is notably *** performed integument transcriptome sequencing of the wild-type strain Dazao and the mutant strains +/bd and bd/*** these experiments,during an early stage of the fourth molt,a stage at which approximately 51% of genes were expressed genome wide（RPKM ≥1） in each strain.A total of 254 novel transcripts were characterized using Cuffompare and BLAST *** of the transcriptome data revealed 28 diffrentially expressed genes（DEGs） that may contribute to bd larval melanism,including 15 cuticular protein genes that were remarkably highly expressed in the bd/bd *** suggest that these signifiantly upregulated cuticular proteins may promote melanism in silkworm larvae.

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The beta-1,4-N-acetylglucosaminidase 1 gene,selected by domestication and breeding,is involved in cocoon construction of Bombyx mori

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The beta-1,4-N-acetylglucosaminidase 1 gene,selected by dome...

中国蚕学会2021年学术年会

作者： Chunlin Li Xiaoling Tong Weidong Zuo Hai Hu Gao Xiong Minjin Han Rui Gao Yue luan kunpeng lu Tingting Gai Zhonghuai Xiang Cheng lu Fangyin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest University

Holometabolous insects have distinct larval,pupal,and adult *** pupal stage is typically immobile and can be subject to predation,but cocoon offers pupal protection for many insect *** cocoon provides a space in which the pupa to adult metamorphosis *** also protects the pupa from weather,predators and *** protein is a precursor of the silk used in cocoon *** used the silkworm as a model species to identify genes affecting silk protein synthesis and cocoon *** used quantitative genetic analysis to demonstrate that-1,4-N-acetylglucosaminidase 1（BmGlcNase1） is associated with synthesis of sericin,the main composite of ***1 has an expression pattern coupled with silk gland development and cocoon shell weight（CSW） variation,and CSW is an index of the ability to synthesize silk ***-regulated expression of BmGlcNase1 increased sericin content by 13.9% and 22.5% while down-regulation reduced sericin content by 41.2% and 27.3% in the cocoons of females and males,*** sequencing revealed that sequence variation upstream of the BmGlcNase1 transcriptional start site（TSS） is associated with the expression of BmGlcNase1 and *** pressure analysis showed that GlcNase1 was differentially selected in insects with and without cocoons（ω1=0.044 vs.ω2=0.154）.This indicates that this gene has a conserved function in the cocooning process of ***1 appears to be involved in sericin synthesis and silkworm cocooning.

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基于实测的海上风电基础局部冲刷及防护措施效果对比研究

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太阳能学报 2023年第12期44卷 230-237页

作者：邱旭马文冠姚中原袁赛杰闫姝卢坤鹏中国华能集团清洁能源技术研究院有限公司北京102209 华能国际电力江苏能源开发有限公司清洁能源分公司南京210015

海上风电单桩基础局部冲刷普遍存在,且极限冲刷深度是开展基础结构设计的重要参数。因此,针对海上风电单桩基础,基于江苏某海上风电场的冲刷实测数据,研究韩海骞公式、王汝凯公式、Qi&Gao公式冲刷计算值与实测值之间的关系;分析对比抛... 详细信息

海上风电单桩基础局部冲刷普遍存在,且极限冲刷深度是开展基础结构设计的重要参数。因此,针对海上风电单桩基础,基于江苏某海上风电场的冲刷实测数据,研究韩海骞公式、王汝凯公式、Qi&Gao公式冲刷计算值与实测值之间的关系;分析对比抛石、固化土及砂被3种冲刷防护措施的防护效果。结果表明:3种计算方法可较为准确预测单桩基础的局部冲刷深度;固化土、砂被、抛石对冲刷防护的效果呈递减趋势。

关键词：海上风电单桩基础局部冲刷冲刷深度冲刷防护冲刷扫测

不同垫块包围角度下砂岩劈裂声发射特性试验研究

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人民长江 2022年第9期53卷 134-139页

作者：许晓通鲁鲲鹏周瑜吴道勇贵州大学资源与环境工程学院贵州贵阳550025 贵州大学喀斯特地质资源与环境教育部重点实验室贵州贵阳550025

为研究岩石劈裂试验中不同垫块包围角度对岩石声发射特性的影响,采用包围度数分别为10°,30°,60°的垫块对砂岩圆盘试样进行单轴循环加卸载试验,对比分析试样的声发射特性。试验结果表明:包围度数的增加对砂岩Kaiser效应几乎没有影响,... 详细信息

为研究岩石劈裂试验中不同垫块包围角度对岩石声发射特性的影响,采用包围度数分别为10°,30°,60°的垫块对砂岩圆盘试样进行单轴循环加卸载试验,对比分析试样的声发射特性。试验结果表明:包围度数的增加对砂岩Kaiser效应几乎没有影响,而试样的累计计数、累计能量计数、峰值强度均显著提高,试样由沿荷载直径附近的单一劈裂破坏逐渐演变为沿垫块端部首先局部失效的复杂破坏。在整个循环加卸载过程中,不同包围度数下砂岩声发射b值演化规律总体一致,不同荷载阶段试样内部损伤的程度不同,b值呈现出一定的演化规律。研究成果可为利用Kaiser效应测量地应力及岩石破裂过程分析提供试验支撑。

关键词：砂岩圆盘试样垫块包围度数 b值声发射 Kaiser效应

Whole-genome Resequencing Reveals Loci under Selection during Silkworm Improvement

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Whole-genome Resequencing Reveals Loci under Selection durin...

中国蚕学会2021年学术年会

作者： Chunlin Li Weidong Zuo Xiaoling Tong Minjin Han Rui Gao Hai Hu kunpeng lu Yue luan Bili Zhang Yanyu Liu Fangyin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest University

＜正＞Breeding or genetic improvement refers to the process of artificial selection following domestication;as such,it has had a major influence on modern agriculture and animal *** generally focuses on traits that gr...

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Deciphering the genetic basis of silkworm cocoon colors provides new insights into biological coloration and henotypic diversification

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Deciphering the genetic basis of silkworm cocoon colors prov...

中国蚕学会2023年学术年会

作者： Yaru lu Jiangwen luo Erxia An Bo lu Yinqiu Wei Xiang Chen kunpeng lu Shubo Liang Hai Hu Minjin Han Songzhen He Jianghong Shen Dongyang Guo Nvping Bu Ling Yang Wenya Xu Cheng lu Zhonghuai Xiang Xiaoling Tong Fangyin Dai State Key Laboratory of Resource Insects Southwest University

The genetic basis of phenotypic variation is a long-standing concern of evolutionary *** has proven to be a visual,easily quantifiable,and highly tractable system for genetic analysis and is an ever-evolving focus of biological *** with the homogenized brownyellow cocoons of wild silkworms,the cocoons of domestic silkworms are spectacularly diverse in color,such as white,green,and yellow-red;this provides an outstanding model for exploring the phenotypic diversification and biological ***,the molecular mechanism underlying silkworm green cocoon formation was investigated,which was not fully *** demonstrated that five of the seven members of a sugar transporter gene cluster were specifically duplicated in the Bombycidae and evolved new spatial expression patterns predominantly expressed in silk glands,accompanying complementary temporal expression;they synergistically facilitate the uptake of flavonoids,thus determining the green ***,polymorphic cocoon coloring landscape involving multiple loci and the evolution of cocoon color from wild to domestic silkworms were analyzed based on the pan-genome sequencing *** was found that cocoon coloration involved epistatic interaction between loci;all the identified cocoon color-related loci existed in wild silkworms;the genetic segregation,recombination,and variation of these loci shaped the multi-colored cocoons of domestic *** study revealed a new mechanism for flavonoids-based biological coloration that highlights the crucial role of gene duplication followed by functional diversification in acquiring new genetic functions;furthermore,the results in this work provide insight into phenotypic innovation during domestication.

关键词： Phenotypic variation Domestication Coloration Flavonoids Sugar transporter

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北洋军阀三大派系的力量对比分析

兰台世界 2017年第3期 92-94页

作者：陆坤鹏东北财经大学马克思主义学院大连116025

时至今日,历史学界对北洋军阀历史的研究可谓汗牛充栋,观之北洋军阀三大派系力量对比的专门研究却鲜有成篇,文章将对直、皖、奉各派军阀的实力作一简单罗列和浅显分析。来新夏先生将北洋军阀时代分为四个阶段,直、皖、奉的分裂混战发生... 详细信息

时至今日,历史学界对北洋军阀历史的研究可谓汗牛充栋,观之北洋军阀三大派系力量对比的专门研究却鲜有成篇,文章将对直、皖、奉各派军阀的实力作一简单罗列和浅显分析。来新夏先生将北洋军阀时代分为四个阶段,直、皖、奉的分裂混战发生在1916至1926年,属第三阶段,三系军阀的力量都经历了各自发展和此消彼长,所以只能分不同时期对各派力量进行纵向的比较分析。三派军阀轮流执政的顺序是皖、直、奉,这也预示着各派的崛起和衰落,文章将以其军事力量的消长为主线叙述这一过程。

关键词：北洋军阀直系皖系奉系军事力量

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突发性聋伴黑棘皮病及格林巴利综合征一例

中华耳鼻咽喉头颈外科杂志 2020年第11期55卷 1072-1074页

作者：鲁鵾鹏熊文萍樊兆民王明明山东大学附属山东省耳鼻喉医院耳内科济南250022 山东大学附属山东省耳鼻喉医院耳科中心济南250022

本文报道了山东大学附属山东省耳鼻喉医院耳内科诊治的1例突发性聋伴黑棘皮病及格林-巴利综合征的患者。患者中年男性,主诉突发右耳听力下降46 d,入院后进行既往病史收集、系统查体、听力学和前庭功能评估、实验室检查及影像学检查,给... 详细信息

本文报道了山东大学附属山东省耳鼻喉医院耳内科诊治的1例突发性聋伴黑棘皮病及格林-巴利综合征的患者。患者中年男性,主诉突发右耳听力下降46 d,入院后进行既往病史收集、系统查体、听力学和前庭功能评估、实验室检查及影像学检查,给予改善内耳微循环、激素和降纤酶等药物治疗和前庭康复训练,治疗12 d,预后差。

关键词：格林巴利综合征突发性聋黑棘皮病前庭功能既往病史系统查体内科诊治耳鼻喉