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Enhancements of non-specific immune response in Mugil cephlus by seaweed extract against Vibrio alginolyticus(BRTR07)

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Journal of Coastal Life Medicine 2015年第10期 776-786页

作者： Rajasekar Thirunavukkarasu Priyadharshini Pandiyan Deivasigamani Balaraman Ilamathi Jayaraman kumaran subaramaniyan Edward Gnana Jothi George Center for Drug Discovery and Development Sathyabama UniversityJeppiaar NagarOld Mamallapuram RoadChennai-600119India CAS in Marine Biology Annamalai UniversityParangipettaiChidambaramTamil Nadu-608502India Department of Microbiology Sri Sankara Arts and Science College(Affiliated to Madras University)EnathurKanchipuram-631561Tamil NaduIndia

Objective:To focus on the growth rate and feed utilization of fish by using trash fish feeds supplement with marine ***:Selected seaweed was extracted using hot-water and its extract was mixed with trash fish feed at different concentrations(0.5%,1%and 2%for 1-30 days)and the non-specific immune response in fish was studied and challenged with Vibrio alginolyticus at 1×106 CFU/*** hot-water extract of seaweeds was analysed by gas chromatography-mass ***:The average body weight(5.320±0.018),percent weight gain(227.66±0.28),specific growth rate(2.080±0.015),hepatosomatic index(1.197±0.00)and viscerosomatic index(4.421±0.150)were significantly increased in the fish feed with seaweed containing 5%of Sargassum wightii(***)when compared with other seaweeds and control ***-water extract of ***(1%)was significantly enhanced the immune response in fish when compared with other diets(0.5%and 2%).*** showed good immunostimulation *** chromatography-mass spectrometry result showed that the hot-water extract of *** seaweed contained fatty ***:Trash fish feed will reduce the production cost and also provide evidence that aqueous leaf extract of ***(1%)was added to a formulated fish diet which could activate the non-specific immune response and disease resistance against Vibrio alginolyticus in Mugil cephalus.

关键词： Mugil cephlus Vibrio alginolyticus Hot-water extract Sargassum wightii Immunostimulation

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Isolation of bioactive compound from marine seaweeds against fish pathogenic bacteria Vibrio alginolyticus(VA09)and characterisation by FTIR

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Journal of Coastal Life Medicine 2013年第1期 26-33页

作者： Rajasekar Thirunavukkarasu Priyadharshini Pandiyan Deivasigamani Balaraman kumaran subaramaniyan George Edward Gnana Jothi Sakthivel Manikkam Balamurugan Sadaiyappan Center of Advance Study in Marine Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608508Tamil NaduIndia

Objective:Fresh marine seaweeds Gracilaria edulis,Gracillaria verrcosa,Acanthospora spicifera,Ulva facita,Ulva lacta(***),Kappaphycus spicifera,Sargassum ilicifolium,Sargassum wightii(***),Padina tetramatica and Padina gymonospora were collected from Mandapam(Rameshwaram,Tamil Nadu)of South East coast of India and were screened for antibacterial ***:All the collected seaweeds were extracted by using five different solvent(methanol,isopropanol,acetone,chloroform,diethyl ether)to study their extracts against fish pathogenic bacteria ***(VA09)purchased from *** minimum inhibition carried out by using Resazurin micro-titre *** extract of *** analysied by ***:The methanolic extract of *** produced a maximum zone of inhibition(1.95±0.11)cm,isopropanol extract maximum inhibition was produced by ***(1.93±0.78)cm,Acetone extract of Gracilaria verrcosa showed maximum zone of inhibition(1.36±0.05)cm,chloroform extract of *** produced a maximum zone(1.56±0.25)cm and diethyl ether extract of *** produced maximum zone of inhibition(1.86±0.11)*** on the antibacterial activity ***,*** and Padina tetramatica showed best antibacterial activity against Vibrio *** this three seaweeds were taken for MIC *** *** methanolic extract,*** diethyl ether extract and Padina tetramatica methanolic extract showed a higher MIC values,and despectively were 25 mg/mL,50 mg/mL and 50 mg/*** result showed that mostly phenolic compounds were present in the ***:Based on the FTIR result *** have high amount of phenolic *** compound have the good antimicrobial *** results clearly show that *** is an interesting source for biologically active compounds that may be applied for prophylaxis and therapy of bacterial fish diseases and it should be used for immunostimulation activity in aquaculture.

关键词： Antibacterial activity Marine seaweeds Vibrio harveyi Resazurin

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Screening of marine seaweeds for bioactive compound against fish pathogenic bacteria and active fraction analysed by gas chromatography-mass spectrometry

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Journal of Coastal Life Medicine 2014年第5期 367-375页

作者： Rajasekar Thirunavukkarasu Priyadharshini Pandiyan kumaran subaramaniyan Deivasigamani Balaraman Sakthivel Manikkam Balamurugan Sadaiyappan George Edward Gnana Jothi Centre of Advance Study in Marine Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608508Tamil NaduIndia Sri Sankara Arts and Science College EnathurKanchipuram-631561Tamil NaduIndia

Objective:To isolate bioactive molecules from marine seaweeds and check the antimicrobial activity against the fish pathogenic ***:Fresh marine seaweeds Gracilaria edulis,Kappaphycus spicifera,Sargassum wightii(***)were *** seaweed was extracted with different *** the study,test pathogens were collected from microbial type culture *** activity was carried out by using disc diffusion method and minimum inhibition concentration(MIC)was *** seaweed was analysed by fourier transform infrared *** cured extract was separated by thin layer chromatography(TLC).Fraction was collected from TLC to check the antimicrobial *** fraction was analysed by gas chromatography mass spectrometer(GCMS).Results:Based on the disc diffusion method,*** showed a better antimicrobial activity than other seaweed *** on the MIC,methanol extract of *** showed lower MIC than other *** were separated by *** this TLC,plate showed a two *** two fractions were separated in preparative TLC and checked for their antimicrobial *** 2 showed best MIC value against the tested *** 2 was analysed by *** on the GCMS,fraction 2 contains n-hexadecanoic acid(59.44%).Conclusions:From this present study,it can be concluded that *** was potential sources of bioactive compounds.

关键词： Marine seaweeds Vibrio sp.fish pathogenic Thin layer chromatography Gas chromatography-mass spectrometry

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