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Stigma Receptivity Is Controlled by Functionally Redundant MAPK Pathway Components in Arabidopsis

Molecular Plant 2020年第11期13卷 1582-1593页

作者： Muhammad Jamshed Subramanian Sankaranarayanan kumar abhinandan Marcus A.Samuel University of Calgary BI 392Department of Biological Sciences2500 University Dr.NWCalgaryAB T2N 1N4Canada Senior Scientist Frontier Agri-Science Inc98 Ontario StreetPort HopeON L1A2V2Canada Department of Botany and Plant Pathology Purdue UniversityWest LafayetteIN 47907USA

In angiosperms,the process of pollination relies on species-specific interaction and signaling between the male(pollen)and female(pistil)counterparts where the interplay between several pollen and stigma proteins decides the fate of the *** Brassicaceae,the dry stigmatic papillary cells control pollen germination by releasing resources only to compatible pollen thereby allowing pollen to hydrate and *** the identification of a number of stigmatic proteins that facilitate pollination responses,the signaling mechanisms that regulate functions of these proteins have remained ***,we show that,in Arabidopsis,an extremely functionally redundant mitogen-activated protein kinase(MAPK)cascade is required for maintaining stigma receptivity to accept compatible *** genetic analyses demonstrate that in stigmas,five MAPK kinases(MKKs),MKK1/2/3/7/9 are required to transmit upstream signals to two MPKs,MPK3/4,to mediate compatible *** functions of these five MKKs in the quintuple mutant(mkk1/2/3RNAi/mkk7/9)phenocopied pollination defects observed in the m pk4RNAi/m pk3 double *** further show that this MAPK nexus converges on Exo70A1,a previously identified stigma receptivity factor essential for *** that pollination is the crucial initial step during plant reproduction,understanding the mechanisms that govern successful pollination could lead to development of strategies to improve crop yield.

关键词： reproduction pollination stigma receptivity compatibility MAPK redundancy Exo70A1 exocytosis phosphorylation

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Disabling of ARC1 through CRISPR–Cas9 leads to a complete breakdown of self-incompatibility responses in Brassica napus

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Plant Communications 2023年第2期4卷 8-11页

作者： kumar abhinandan Neil M.N.Hickerson Xingguo Lan Marcus A.Samuel University of Calgary Department of Biological Sciences2500 University Drive NWCalgaryAB T2N 1N4Canada 20/20 Seed Labs Inc. NiskuAB T9E 7N5Canada Key Laboratory of Saline-Alkali Vegetation Ecology Restoration Ministry of EducationCollege of Life SciencesNortheast Forestry UniversityHarbin 150040China

Dear Editor,Many flowering plants utilize the self-incompatibility(SI)response as a genetic mechanism to prevent self-pollen from establishing on the stigmas,thereby promoting outcrossing and genetic *** Brassica during SI,recognition of the pollen ligand SP11 by S-locus receptor kinase(SRK)results in activation of the E3 ligase ARM-Repeat-Containing protein(ARC1),which leads to proteasomal degradation of compatibility factors required for successful pollen acceptance.

关键词： utilize breakdown thereby

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Flavonoids and ROS Play Opposing Roles in Mediating Pollination in Ornamental Kale （Brassica oleracea var. acephala）

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Molecular Plant 2017年第10期10卷 1361-1364页

作者： Xingguo Lan Jia Yang kumar abhinandan Yuzhe Nie Xiaoyu Li Yuhua Li Marcus A. Samuel College of Life Sciences Northeast Forestry University Harbin Heilongjiang 150040. P R China Department of Biological Sciences University of Calgary Calgary AB T2N 1N4. Canada

Dear Editor,In most flowering plants, the stigma of the flower represents the barrier that prevents any unrelated or incompatible （genetically similar） pollen from germination and fertilizing the ovule, leading to either defective or genetically unfavorable embryos. In Brassicaceae, self-incompatibility （SI） is a genetic mechanism in the stigmas that can discern compatible versus incompatible mate （pollen）, in turn, rejecting self or incompatible pollen, while allowing non-self or compatible pollen to develop successfully to fertilize the ovules. Thus, stigmas have evolved complex mechanisms and specialized proteins to reject incompatible mates and to recognize and accept compatible pollen （Doucet et al., 2016）. The fact that SI has been shown to exclusively operate in the stigmas temporally before and during anthesis （Kandasamy et al., 1993） indicates that multiple metabolic pathways essential for pollination exist in these stigmatic papillary cells.

关键词： Flavonoids ROS Play Opposing Roles Mediating Pollination Ornamental Kale Brassica oleracea var acephala

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Surface defect engineering of metal oxides photocatalyst for energy application and water treatment

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Journal of Materiomics 2021年第2期7卷 388-418页

作者： Pankaj Raizada Vatika Soni abhinandan kumar Pardeep Singh Aftab Aslam Parwaz Khan Abdullah M.Asiri Vijay kumar Thakur Van-Huy Nguyen Advance School of Chemical Sciences Shoolini UniversitySolanHP173229India Center of Excellence for Advanced Materials Research King Abdulaziz UniversityP.O.Box 80203Jeddah21589Saudi Arabia Chemistry Department Faculty of ScienceKing Abdulaziz UniversityP.O.Box 80203Jeddah21589Saudi Arabia Biorefining and Advanced Materials Research Centre Scotland Rural College(SRUC)EdinburghUnited Kingdom Institute of Research and Development Duy Tan UniversityDa Nang550000Viet Nam

Despite metal oxides offer excellent characteristics in the field of photocatalysis,they often suffer from charge carrier recombination as well as limited visible response,which indeed reduce the charge kinetics process and ultimately reduce the photocatalytic *** engineering is a sophisticated technique to manufacture defects and alter the geometric structure and chemical environment of the *** present study provides an all-inclusive outline of recent developments on the classification of metal oxide defects based on the dimensions of a host crystal ***,surface modification of metal oxides through 0D(point),1D(line),2D(planar),and 3D(volume)defects with their subsequent mechanism and impact on photocatalytic performance are *** wisely amending the morphology(cores along with the shells)and electronic structure of metal oxide photocatalysts(TiO_(2),ZnO,Bi_(2)O_(3),Fe_(2)O_(4) etc.)through different attuned and veritable approaches,their photocatalytic activity can be substantially *** studies on defect engineering not only expose the altered physicochemical features but also modulate the electron-hole pair dynamics,stability,and active radical production for various photoredox *** atomic,as well as electronic configuration,facilitated a photocatalyst material to have different optical features,adsorption properties along with improved carrier transfer as well as isolation ***,the systematic exploration of photocatalytic rudiments of defect rich metal oxide for various applications such as H_(2) evolution,CO_(2) reduction,pollutant degradation,and bacterial disinfection could bring significant research advancement in this field.

关键词： Defect engineering Nanostructured metal oxides Photocatalysis Surface reactions Point defects Dislocations Boundaries Voids

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Sulphur vacancy defects engineered metal sulfides for amended photo(electro)catalytic water splitting: A review

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Journal of Materials Science & Technology 2023年第21期152卷 50-64页

作者： Kusum Sharma abhinandan kumar Tansir Ahamad Quyet Van Le Pankaj Raizada Archana Singh Lan Huong Nguyen Sourbh Thakur Van-Huy Nguyen Pardeep Singh School of Advanced Chemical Sciences Shoolini UniversitySolanHimachal Pradesh 173212India Department of Chemistry College of ScienceKing Saud UniversitySaudi Arabia Department of Materials Science and Engineering Korea University145AnamroSeongbuk-guSeoul 02841Republic of Korea Advanced Materials and Processes Research Institute Hoshangabad RoadBhopalMP 462026India Faculty of Biology and Environment Ho Chi Minh City University of Food Industry(HUFI)140 Le Trong Tan StreetTay Thanh WardTan Phu DistrictHo Chi Minh CityVietnam Department of Organic Chemistry Bioorganic Chemistry and BiotechnologySilesian University of TechnologyB.Krzywoustego 444-100 GliwicePoland Faculty of Allied Health Sciences Chettinad Hospital and Research InstituteChettinad Academy of Research and EducationKelambakkam-603103Tamil NaduIndia

Vacancy engineering in metal sulfides has garnered enormous attention from researchers because of their outstanding ability to modulate the optical and physiochemical properties of ***,in the case of sulfides,the catalytic activity is drastically hindered by the quick reassembly of excitons and the photocorrosion *** designing and generating S-vacancies in metal sulfides has emerged as a potential strategy for attaining adequate water splitting to generate H_(2) and O_(2) because of the simulta-neous improvement in the optoelectronic ***,developing efficient catalysts that manifest optimal photo(electro)catalytic performance for large-scale applicability remains ***,it is of utmost interest to explore the insightful features of creating S-vacancy and study their impact on catalytic *** review article aims to comprehensively highlight the roles of S-vacancy in sulfides for amended overall water-splitting *** photocatalytic features of S-vacancies modulated metal sulfides are deliberated,followed by various advanced synthetic and characterization techniques for effectual generation and identification of vacancy *** specific aspects of S-vacancies in refin-ing the optical absorption range charge carrier dynamics,and photoinduced surface chemical reactions are critically examined for overall water splitting ***,the vouchsafing outlooks and op-portunities confronting the defect-engineered(S-vacancy)metal sulfides-based photocatalysts have been summarized.

关键词： S-vacancy Photo(electro)catalysis Metal sulfides Water splitting Engineering Identification of S-vacancies

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Electrochemical sensor for dopamine detection based on multiwalled carbon nanotube/molybdenum disulfide quantum dots with machine learning integration and anti-interference capability

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Tungsten 2025年第1期7卷 137-151页

作者： Geethukrishnan Onkar Apte Kiran kumar Tadi Energy Electrodics and Electrocatalysis (EEE) Lab Department of Chemistry School of Advanced Sciences Vellore Institute of Technology School of Computer Science and Engineering Vellore Institute of Technology

The precise detection and measurement of dopamine（DA）,a crucial neurotransmitter in the human body,plays a significant role in diagnosing,preventing,and treating neurological diseases associated with its levels.A highly sensitive DA electrochemical sensor was constructed by combining molybdenum disulfide quantum dots（MSQDs） with multiwalled carbon nanotubes（MWCNTs）.The MSQDs were synthesized using the shear exfoliation *** sensors consist of MSQDs with Mo-S edge catalytic centers for the DA redox reaction,and MWCNTs amplify the sensor *** linearity of the sensor for the detection of DA was tested in the presence of ascorbic acid(AA,50 μmol·L-1) and uric acid(UA,200 μmol·L-1),and exhibited linearity from 2 to 966 μmol·L-1of DA with 0.097 μA(mol·L-1)-1sensitivity and a low limit of detection of0.6 μmol·L-1（the ratio between signal and noise,S/N=3）.Moreover,the sensitivity and selectivity of the sensor were also studied using *** is no increase in amperometric current after adding the most potentially interfering *** sensor was successfully applied to recover DA in human blood sera ***,machine learning algorithms were operated to aid in the near-precise detection of DA in the heterogeneous mixture containing AA and *** algorithms facilitate the identification and quantification of DA amidst coexisting interferents,including AA,that are commonly present in biological matrices.

关键词： Biosensor Complex mixture Dopamine Machine learning MoS2 Multiwalled carbon nanotubes

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Translocator protein and neurodegeneration: insights from Alzheimer’s disease

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Neural Regeneration Research 2025年第4期20卷 1090-1091页

作者： Arpit kumar Pradhan Rainer Rupprecht Gerhard Rammes Klinik für Anaesthesiologie und Intensivmedizin der Technischen Universität München Klinikum rechts der IsarMunichGermany Graduate School of Systemic Neurosciences Ludwig-Maximilians-Universität MünchenMartinsriedGermany Department of Psychiatry and Psychotherapy University RegensburgRegensburgGermany

The 18 kDa translocator protein(TSPO)located on the outer mitochondrial membrane regulates several key cellular processes including mitochondrial homeostasis,cholesterol transport,apoptosis,cell proliferation,and maintenance of mitochondrial health(Rupprecht et al.,2022,2023).TSPO is expressed in both peripheral organs and the central nervous system,with a more pronounced expression in tissues that produce *** main reason why TSPO has garnered so much attention is because it plays a key role in neurosteroidogenesis by transferring cholesterol from the outer to the inner mitochondrial membrane,which is the rate-limiting step in neurosteroid synthesis.A cholesterol-recognizing amino acid consensus domain has been identified in the cytosolic C terminus of the TSPO protein by both in vitro and site-directed mutagenesis experiments(Li et al.,2001).However,the role of TSPO in the process of neurosteroid synthesis has been challenged by several studies,particularly TSPO knockout models,which suggest that TSPO removal does not affect the phenotype or the system’s viability(Tu et al.,2014).However,ligands targeting TSPO have been shown to enhance levels of neurosteroids which suggests that neurosteroidogenesis is one of the major functional roles mediated by the TSPO protein.

关键词： cholesterol organs

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Congruent Feature Selection Method to Improve the Efficacy of Machine Learning-Based Classification in Medical Image Processing

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 357-384页

作者： Mohd Anjum Naoufel Kraiem Hong Min Ashit kumar Dutta Yousef Ibrahim Daradkeh Department of Computer Engineering Aligarh Muslim UniversityAligarh202002India College of Computer Science King Khalid UniversityAbha61413Saudi Arabia School of Computing Gachon UniversitySeongnam13120Republic of Korea Department of Computer Science and Information Systems College of Applied SciencesAlMaarefa UniversityRiyadh13713Saudi Arabia Department of Computer Engineering and Information College of Engineering in Wadi AlddawasirPrince Sattam bin Abdulaziz UniversityAl-Kharj16273Saudi Arabia

Machine learning(ML)is increasingly applied for medical image processing with appropriate learning *** applications include analyzing images of various organs,such as the brain,lung,eye,etc.,to identify specific flaws/diseases for *** primary concern of ML applications is the precise selection of flexible image features for pattern detection and region *** of the extracted image features are irrelevant and lead to an increase in computation ***,this article uses an analytical learning paradigm to design a Congruent Feature Selection Method to select the most relevant image *** process trains the learning paradigm using similarity and correlation-based features over different textural intensities and pixel *** similarity between the pixels over the various distribution patterns with high indexes is recommended for disease ***,the correlation based on intensity and distribution is analyzed to improve the feature selection ***,the more congruent pixels are sorted in the descending order of the selection,which identifies better regions than the ***,the learning paradigm is trained using intensity and region-based similarity to maximize the chances of ***,the probability of feature selection,regardless of the textures and medical image patterns,is *** process enhances the performance of ML applications for different medical image *** proposed method improves the accuracy,precision,and training rate by 13.19%,10.69%,and 11.06%,respectively,compared to other models for the selected *** mean error and selection time is also reduced by 12.56%and 13.56%,respectively,compared to the same models and dataset.

关键词： Computer vision feature selection machine learning region detection texture analysis image classification medical images

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Market Drivers in India’s Smart Grid:Responsibilities and Roles of Stakeholders

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Energy Engineering 2025年第1期122卷 101-128页

作者： Abhay Sanatan Satapathy Suresh kumar Sahoo Asit Mohanty Yasser Fouad Manzoore ElahiMohammad Soudagar Erdem Cuce Faculty of Management Studies Sri Sri UniversityCuttack754006India Institute of Power Engineering National Energy UniversityJalan Ikram-UnitenKajangSelangor43000Malaysia Centre of Research Impact and Outcome Chitkara UniversityRajpura140417PunjabIndia Department of Applied Mechanical Engineering College of Applied EngineeringMuzahimiyah BranchKing Saud UniversityRiyadh11421Saudi Arabia College of Engineering Lishui UniversityLishui323000China Department of Mechanical Engineering Graphic Era(Deemed to be University)Dehradun248002UttarakhandIndia Department of Mechanical Engineering Faculty of EngineeringUniversity of MalayaKuala Lumpur50603Malaysia Department of Mechanical Engineering Faculty of Engineering and ArchitectureRecep Tayyip Erdogan UniversityZihni Derin CampusRize53100Turkey Center for Research Impact&Outcome Chitkara UniversityRajpura140401PunjabIndia University Centre for Research and Development Chandigarh UniversityMohali140413PunjabIndia

The emergence of smart grids in India is propelled by an intricate interaction of market dynamics,regulatory structures,and stakeholder *** study analyzes the primary factors that are driving the widespread use of smart grid technologies and outlines the specific roles and obligations of different stakeholders,such as government entities,utility companies,technology suppliers,and *** activities and regulations are crucial in facilitating the implementation of smart grid technology by offering financial incentives,regulatory assistance,and strategic *** firms have the responsibility of implementing and integrating smart grid infrastructure,with an emphasis on improving the dependability of the grid,minimizing losses in transmission and distribution,and integrating renewable energy *** companies offer the essential hardware and software solutions,which stimulate creativity and enhance *** actively engage in the energy ecosystem by participating in demand response,implementing energy saving measures,and adopting distributed energy resources like solar panels and electric *** study examines the difficulties and possibilities in India’s smart grid industry,highlighting the importance of cooperation among stakeholders to build a strong,effective,and environmentally friendly energy future.

关键词： Smart grid stakeholders smart grid technology market drivers

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Nano-Enhanced Dairy Scum Biodiesel Blends:Unleashing the Power of Aluminium Oxide for Sustainable and Efficient Engine Performance

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Journal of Thermal Science 2025年第1期34卷 283-302页

作者： Veeranna MODI Prasad B.RAMPURE Sunil kumar SHETTY Madeva NAGARAL Channa Keshava NAIK N Johar MGM Muhammad Irsyad ABDULLAH Abdullah I.AL-MANSOUR Shamshad ALAM KLE College of Engineering and Technology ChikodiVisvesvaraya Technological University Nitte (Deemed to be University) NMAM Institute of Technology (NMAMIT)Department of Mechanical Engineering Aircraft Research and Design Centre HAL Department of Mechanical Engineering BGS College of Engineering and TechnologyBengaluruAffiliated to Visvesvaraya Technological University Management and Science University Department of Civil Engineering College of EngineeringKing Saud University

This study investigates the effect of aluminium oxide nanoparticlesaddition to biodiesel blends made from dairy milk scum on performance and combustion characteristics of the diesel *** dispersion of Al2O3nanoparticles in B20 blends at different concentrations was done with the help of *** number of blends were prepared for the *** Machine learning algorithms(Random Forest（RF）and CatBoost) was used for the *** results show that in comparison to biodiesel blends without nanoparticles,the kinematic viscosity and density is higher for fuel blends with *** these Fuel blends have higher calorific *** blends exhibited reduced Brake Specific Fuel Consumption（BSFC） of2.85% than the blends without nano particles(Dairy Scum Methyl Ester Biodiesel 20%+Neat Diesel 80%（in volume）,DSMEB20),57.14% less CO,40.8% less hydrocarbon,and increased NOxemissions compared to conventional diesel,contributing to the development of environmentally friendly and renewable biofuel blends with *** B20NP30 is the optimal blend for performance and emission *** study concludes with findings on enhanced Brake Thermal Efficiency（BTE） of 26.29% in 3×10-5（in volume） Al2O3nanoparticle-blended DSME B20 and other DSME B20 fuel blends,emphasizing the importance of optimal nanoparticle *** correlation matrix shows how engine load,efficiency measures（BTE,BSFC）,and emissions（CO,CO2,NOx,Smoke） are connected in complex *** results help us understand the complicated dynamics of engine performance and emission characteristics ***'s diagram for BTE and BSFC shows that CatBoost-based BTE models perform superior to RF-based models during the training as well as testing *** results were obtained for CO and CO2emission results.

关键词： Al2O3 concentration CI engine Dairy Scum Methyl Ester Biodiesel

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